2 results about "Heparinase" patented technology

The application belongs to the technical field of heparin extraction, and particularly relates to a method for preparing low-molecular heparan sulfate by using an enzyme method. The method comprises the following steps: (1) dissolving heparan sulfate in deionized water, adjusting the pH to 8-8.5 by using a NaOH solution at 20-25 DEG C, adding modified carbon nanotubes loaded with heparinase, then performing enzymolysis for 3.0-3.5 h under stirring at 35-45 DEG C, and obtaining an enzymolysis solution; (2) when the specific absorbance of the enzymolysis solution in step (1) is 1.3-1.5 at a wavelength of 230 nm, rapidly heating the enzymolysis solution to 95-98 DEG C, and terminating the reaction after 0.5-1.0 h; and (3) cooling the enzymolysis solution after the reaction to 20-30 DEG C, adding anhydrous ethanol, stirring for 1-2 h, centrifuging to obtain a precipitate, adding deionized water, filtering by using a 0.1-0.22 mu m filter membrane, and vacuum freeze-drying to obtain low-molecular heparan sulfate. The application has a mild reaction and a high yield.