Detection method for evaluating curative effect and residual condition of heparin drugs

A detection method, heparin technology, applied in the direction of biological testing, material inspection products, etc., can solve the problems of short validity period, poor stability, poor detection accuracy, etc., and achieve the effect of simple operation, improved stability, improved stability and accuracy

Inactive Publication Date: 2018-01-09
北京乐普诊断科技股份有限公司
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Problems solved by technology

[0005] The purpose of the present invention is to provide a detection method for detecting and evaluating the curative effect and residue of heparin, low molecular weight heparin and heparin-like drugs, so as to solve the problems of poor stability, short validity period and poor detection accuracy existing in the existing detection

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  • Detection method for evaluating curative effect and residual condition of heparin drugs

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[0028] The specific implementation manners of the present invention will be further described in detail below in conjunction with the accompanying drawings and examples. The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

[0029] As shown in the figure, a detection method for evaluating the curative effect and residual situation of heparin drugs includes the following steps:

[0030] S1. Prepare a heparinase solution and a protective agent solution in a volume ratio of 1:100-300, more preferably 1:100-150.

[0031] Wherein, the enzyme activity of the heparinase solution in step S1 is 12,000 U / ml, and the protective agent solution mainly contains trehalose, mannitol, sucrose, lactose, polyethylene glycol, sodium acetate, ammonium sulfate, sodium chloride , calcium chloride, and one or more of them, the mass ratio of the protective agent in the mixed solvent is 1-15%. One of sodium azide, genta...

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Abstract

The invention relates to the field of blood detection, in particular to a detection method for evaluating the curative effect and the residual condition of heparin drugs. The heparin drugs mainly comprise heparin, low-molecular-weight heparin and some heparinoid drugs. The detection method mainly comprises steps as follows: heparinase, a freeze-drying protective agent and an adhesive are mixed, then a mixed reagent in appropriate proportion is prepared from an obtained mixture by the aid of a buffer solution, the mixed reagent is enveloped in a test-cup and subjected to freezing and vacuum pumping treatment, and a heparinase cup is obtained. The detection method has a plurality of advantages that the operation is simple, the guarantee period is long, the stability is good and the like. Theheparinase cup and a common cup (a blank sample cup) are put in a thrombelastogram, an anticoagulant and a chelating agent are added to the cups respectively, endogenously activated whole blood samples are added to the test-cups, and thromboelastography is started for detection; values R of starting time of blood clot formation in the heparinase cup and the common cup are compared, and whether heparin exists in the blood can be judged; if heparin exists, the value R of the starting time of blood clot formation in the heparinase cup is smaller than that of the starting time of blood clot formation in the common cup. The method is simple to operate, a detection result is good in repeatability, and the accuracy is high.

Description

technical field [0001] The invention relates to the technical field of blood detection, in particular to a detection method for assessing curative effect and residue of heparin, low molecular weight heparin and heparin-like drugs by thromboelastography. Background technique [0002] Heparanase refers to a type of polysaccharide lyase that can specifically cleave the glycosidic bonds of heparin and heparin-like main chains. It was first discovered and isolated from Flavobacterium heparinus, and later found in some microorganisms and animal tissues. Heparanase is present. At present, there are three kinds of heparanases that have been isolated and purified, namely heparanase I, II and III, and the enzymatic properties of these three enzymes are different. Among them, heparinase I is widely used, such as: removing residual heparin in blood, preventing blood coagulation, producing low molecular weight heparin, studying the structure of heparin, etc. In 1948, German Harter inve...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/49
Inventor 李阳魏明明邱笑违董飒英
Owner 北京乐普诊断科技股份有限公司
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