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Unhairing enzyme preparation and unhairing method using same

A depilatory enzyme and preparation technology, applied in the field of tanning enzyme depilation technology, can solve the problems of loose surface, rotten surface, affecting the mechanical properties of finished leather, etc., achieve strong removal effect, reduce pollution, and realize the effect of clean production

Active Publication Date: 2019-11-05
QILU UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Enzymatic depilation is a major research hotspot in the tanning industry in recent years, but no substantial progress has been made. The reason is that when the enzyme acts on the hair follicle or hair root, it will cause problems such as loose surface and rotten surface, and will also damage the skin. Collagen fibers have a certain impact, thereby affecting the mechanical properties of the finished leather. In addition, the existing enzymes have strict requirements on temperature and pH, which limits their application.

Method used

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  • Unhairing enzyme preparation and unhairing method using same
  • Unhairing enzyme preparation and unhairing method using same
  • Unhairing enzyme preparation and unhairing method using same

Examples

Experimental program
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Effect test

Embodiment 1

[0034] Example 1, a depilatory enzyme preparation prepared by compounding heparanase, lipase and WB 600 / KerT keratinase at an enzyme activity ratio of 3:3:5.

[0035] The WB 600 / KerT keratinase is derived from Bacillus, and its activity is 5000-100000 U / g; WB600 / KerT keratinase acts on goat leather at an enzyme concentration of 30-1000 U / mL, and the enzyme can also act on cowhide and pigskin.

[0036] The heparinase is derived from Bacillus, which has a high activity of degrading heparin, and its heparin activity is 100-3000 U / L; it acts on goat leather at an enzyme concentration of 100-1000 U / L, and the enzyme can also act on cowhide and pigskin.

[0037] In addition to WB600 / KerT keratinase, heparanase and lipase, trace amounts of sodium sulfide can also be selectively added in its application process. The amount of sodium sulfide added is 0.05%-0.5% of the skin weight, and it is added 3-8 hours after the action of the enzyme preparation.

[0038] The application method o...

Embodiment 2

[0039] Embodiment 2, a kind of method of applying this enzyme preparation to remove goat fur, comprises the following steps:

[0040] (1) Choose goat skin, conduct routine immersion and de-flesh treatment.

[0041] (2) Hair removal treatment: carried out in a drum, the water consumption is 60% of the tare weight, and the hair removal enzyme contains 20 U / mL of heparanase, 50 U / mL of keratinase, and 70 U / mL of lipase. After reacting for 3 hours, add 0.05% sodium sulfide by tare, and react for 1 hour.

[0042] (3) Conventional liming and pickling operations, and weighing of pickled skins.

[0043] (4) The weight of the pickled leather is increased by 30%, and the aldehyde tanning (ie glutaraldehyde) and vegetable tanning are carried out. The order of adding the two tanning agents can be changed mutually.

Embodiment 3

[0044] Embodiment 3: a kind of method of applying this enzyme preparation to go off yellow cattle fur, comprises the following steps:

[0045] (1) Select yellow cowhide for conventional soaking and de-fleshing treatment.

[0046] Hair removal treatment: carried out in a drum, the amount of water used is 40% of the skin weight, and the hair removal enzyme contains 30 U / mL of heparanase, 80 U / mL of keratinase, and 50 U / mL of lipase. After drum depilation for 5 hours, add 0.5% lime and let stand overnight.

[0047] (2) Routine liming and pickling operations, and weighing of pickled skins.

[0048] (3) The weight of the pickled leather is increased by 30%, and the aldehyde tanning (ie glutaraldehyde) and vegetable tanning are carried out. The order of adding the two tanning agents can be changed mutually.

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Abstract

The invention discloses an unhairing enzyme preparation and an unhairing method using the same, and the unhairing enzyme preparation is prepared by compounding heparinase, lipase and WB 600 / KerT keratinase according to an enzyme activity ratio of 3: 3: 5. The unhairing enzyme preparation has the following technical effects: the unhairing enzyme preparation can effectively solve the pollution caused by traditional sulfide unhairing, effectively acts on original fur hair through heparinase, keratinase and lipase, greatly reduces the dosage of sodium sulfide in the unhairing process, and reducesthe treatment cost of wastewater, and reduces sulfide emissions, thereby reducing environmental pollution and further realizing cleaner production in depilation process.

Description

technical field [0001] The invention relates to the field of tanning enzyme dehairing technology, in particular to the preparation of a depilatory enzyme preparation, a preparation method and its application, which can be used for depilatory treatment of sheepskin, cowhide and pigskin. Background technique [0002] In the process of tanning, sulfide and lime are often used for hair removal, but sulfide and lime cause serious harm to the environment. [0003] The hydrolysis of sulfide to hair causes a substantial increase in the content of organic matter in wastewater, which not only causes damage to water resources, but also wastes keratin resources due to the difficulty in recycling the hair generated during the depilation process. In addition, a large amount of organic matter in the water also increases Enterprises' treatment costs for wastewater. Therefore, a series of enzymatic hair removal methods have been developed and utilized. [0004] Enzymatic depilation is a ma...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C14C1/06
CPCC14C1/065
Inventor 曹珊宋金枝李彦春
Owner QILU UNIV OF TECH
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