61 results about "Nano composites" patented technology

The invention relates to a screening method and an activity evaluation method of a natural antioxidant, and the screening method is characterized in that a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant reaction system is constructed, and the activity of the natural antioxidant is evaluated through a high performance liquid chromatography-ultraviolet detector-tandem mass spectrometry analysis technology. Screening of the natural antioxidant is realized; according to the activity evaluation method, a cerium dioxide-based nano composite material with a specific structure is used as oxidase, a nano oxidase + natural antioxidant + TMB reaction system is constructed, and the activity evaluation of the natural antioxidant is realized through an oxidized TMB photo-thermal detection technology. According to the method disclosed by the invention, the used reaction system is high in applicability, high in accuracy and good in selectivity.

The invention belongs to the technical field of biomedical materials, and particularly relates to application of a HAp-MXene nano composite material in regulation and control of an osteogenesis immune microenvironment. The HAp and MXene nanocomposite is obtained by combining hydroxyapatite nanorods and MXene nanosheets, the HAp and MXene nanocomposite is interfered by adjusting an external magnetic field, so that the HAp and MXene nanocomposite shows one effect of regulating and controlling polarization of macrophage M1 or macrophage M2; then, the HAp-MXene nanocomposite is implanted into a bone defect area, and in the initial stage of the operation, the magnetoelectric effect of MXene is excited by applying a rotating magnetic field, polarization of M1 type macrophages is promoted, proinflammatory factors are generated, and bacterial infection is inhibited; then, in the middle stage, through removing a magnetic field, releasing calcium ions, promoting polarization of the M2 type macrophages, relieving inflammatory response and promoting bone regeneration, programmed regulation and control of the macrophages are achieved in different repair stages.

The invention belongs to the technical field of photoacoustic response antibacterial dressings, and discloses PDA (at) Ag (at) Ag2S / SA / F68 / F127 antibacterial temperature-sensitive hydrogel as well as a preparation method and application thereof.The PDA (at) Ag (at) Ag2S / SA / F68 / F127 antibacterial temperature-sensitive hydrogel can complete conversion of gel and sol under the condition of 4-60 DEG C, PDA (at) Ag (at) Ag2S nano-enzyme is constructed by means of an in-situ growth method, a PDA (at) Ag (at) Ag2S nano-composite material can generate electron-hole pairs under photoacoustic combination, then reactive oxygen species (ROS) is generated, and the antibacterial temperature-sensitive hydrogel can be used for preparing antibacterial temperature-sensitive hydrogel. The photoacoustic response antibacterial effect is realized. Meanwhile, PDA (at) Ag (at) Ag2S has peroxidase (POD)-like activity, and the material can catalyze endogenous hydrogen peroxide (H2O2) in a common wound microenvironment with the pH value of 7-8 to generate reactive oxygen species (ROS) with high toxicity, such as hydroxyl free radicals (. OH), so that efficient killing of various bacteria under photoacoustic combination is realized. Synthesis conditions are mild, and the process is simple, economical and feasible. The hydrogel has thermosensitivity, can resist inflammation, stop bleeding and relieve wound pain, does not loosen after absorbing seepage, is free of residues and does not adhere to a wound surface when being removed, and a new way is provided for developing novel medical dressings for treating drug-resistant bacterial infection wounds.

The present invention belongs to the fields of novel nanocomposites, immunoassays, and biosensor technologies, and provides a method for preparing a sandwich-type electrochemical immunosensor for cardiac troponin I. The present invention uses highly conductive, gold-particle-loaded, hierarchically porous nitrogen-doped carbon nanotubes (Au NPs / N-CNTs) as a substrate and mesoporous palladium nitrogen nanocubes (meso-PdN NCs) as an electrochemical signal amplification platform to achieve quantitative detection of cardiac troponin I (CTnI) antigen. This method has the advantages of strong specificity, high sensitivity, and a low detection limit, and has important scientific significance and application value for the detection of acute myocardial infarction (AMI).

The application discloses a method for treating antibiotic wastewater by using a graphdiyne / ferrous indium sulfide nanocomposite to activate persulfate, and the method is characterized by using the graphdiyne / ferrous indium sulfide nanocomposite as a catalyst to activate persulfate for degrading and treating antibiotic wastewater, wherein the graphdiyne / ferrous indium sulfide nanocomposite comprises ferrous indium sulfide nanoparticles, and the nanoparticles are loaded with graphdiyne nanosheets. The method for treating antibiotic wastewater by using the graphdiyne / ferrous indium sulfide nanocomposite to activate persulfate uses the graphdiyne / ferrous indium sulfide nanocomposite as a catalyst, wherein the nanocomposite has many reactive sites, high catalytic activity and good stability, can efficiently activate persulfate with a smaller amount of catalyst, can efficiently remove antibiotics in wastewater, and has the advantages of low cost, high treatment efficiency, good removal effect, green environmental protection and the like, and therefore, the method has important significance for effectively purifying antibiotic wastewater, has high use value and good application prospect.

The present application provides the use of a nanocomposite with a core-shell structure. Specifically, the present application provides the use of a nanocomposite with a core-shell structure in the preparation of a drug for preventing and / or treating Clostridium difficile infection and recurrent diseases caused thereby, wherein the nanocomposite comprises: ZnO nanoparticles as a core; mesoporous silica as a shell coated on the surface of the ZnO nanoparticles; an antibiotic loaded in the pore channel of the mesoporous silica; hyperbranched polylysine modified on the surface of the mesoporous silica; and a pH-responsive coating material as the outermost layer. The nanocomposite provided by the present application can achieve precise targeted release of the drug in the colon, and through the sequential and synergistic mechanism of "membrane breaking-sterilization-spore inhibition-repair", it can simultaneously solve multiple factors leading to CDI recurrence such as biofilm protection, spore storage, bacterial population destruction, mucosal damage, etc., and improve the treatment effect.

An electrochemical aptamer sensor for detecting GP73 is constructed based on a reducing graphene oxide-ferrocene-polyaniline rGO-Fc-PANi nanocomposite. The rGO-Fc-PANi has a large specific surface area, high conductivity and good in-situ electrochemical activity, and is used as a carrier to combine with GP73 Apt The signal probe is formed by coupling, and the cDNA is supplemented by the aptamer to combine with GP73 Apt The cDNA-GP73 is formed by hybridization Apt The double-stranded GP73 protein specifically combines with GP73 Apt A competitive relationship is formed with the cDNA, and the change in the redox peak current of Fc in the rGO-Fc-PANi is recorded by a DPV method, so that the detection of GP73 is realized. The method has low cost, good selectivity and high sensitivity, and the minimum detection limit is 0.15 pg / mL.

The invention discloses a lactic acid-responsive nano composite material for antitumor chemotherapy-immunotherapy. The structure of the nano composite material is a nano structure formed by compounding PEGylated viral vaccine protein, lactate oxidase and hollow manganese dioxide loaded with a hypoxia-sensitive chemotherapeutic drug; the nano composite material provided by the invention can simultaneously induce a tumor to release a large amount of antigens, promote an antigen cell presentation process and improve the tolerance of the tumor to immunotherapy, can efficiently activate natural immune response of a tumor part, realizes a tumor inhibition effect, can regulate and control natural immunity of the tumor from multiple aspects, and has a wide application prospect. And safe and efficient anti-tumor chemotherapy-immunotherapy is realized.

The application discloses a preparation method of a multi-charge electrostatic self-assembled nanocomposite and application thereof, and relates to the technical field of nanomaterials. The method comprises the following steps: (1) synthesizing CAT-Ce6; (2) synthesizing negative electric Ag2S-3MPA QDs; (3) synthesizing positive electric Ag2S-NH2 QDs; and (4) preparing Ag2S@CAT-Ce6@Oxa nanocomposite: ultrasonic mixing of Ag2S-NH2 QDs solution and CAT-Ce6 solution to obtain a mixed solution, dropwise adding Oxa dissolved in a mixture of methanol and DMSO into the mixed solution, stirring in a dark environment at room temperature to obtain Ag2S@CAT-Ce6@Oxa crude product, and purifying the Ag2S@CAT-Ce6@Oxa crude product to obtain Ag2S@CAT-Ce6@Oxa nanoparticle pure product. The application is helpful to realize controllable release of drugs, improve curative effect, effectively relieve tumor hypoxia in vivo, provide a better environment for photodynamic therapy and other treatments, realize accurate positioning and observation of tumors, effectively inhibit tumor growth and prolong survival time, improve curative effect and reduce side effects, and has good biocompatibility and wide application prospect in vivo.

The present disclosure provides a nanocomposite and a preparation method and use thereof. In the present disclosure, the nanocomposite is wrapped with Prussian blue nanoparticles (PB) using a platelet membrane (PM) as a shell; and a surface of the PM is modified with an aptamer of cancer cells and horseradish peroxidase (HRP). An ability of platelets (PLTs) to specifically target cancer cells and inflammatory sites can effectively enhance the accumulation of nanoparticles at tumor sites, and help PB better achieve a desirable photothermal therapy (PTT) under near-infrared light irradiation. In addition, hydrogen peroxide is highly expressed in the tumor microenvironment; the HRP modified on a surface of the nanocomposite can decompose the hydrogen peroxide to generate oxygen bubbles, which drive active transport of the nanocomposite, thereby enhancing the accumulation in cancer cells. Modification with the aptamer of cancer cells on a platelet membrane surface enhances cancer cell targeting.

The application belongs to the field of biosensors, and provides a construction method and application of an electrochemical biosensor for quantifying AFP-L3% (AFP-L3 / AFP), and the specific process is as follows: Au@UiO-66 nanocomposites are obtained by using a hydrothermal method and an immersion reduction method, TCEP can open S-S in alpha-fetoprotein to obtain sulfydryl (-SH) and connect to Au@UiO-66, in-situ synthesis of AFP aptamer-copper clusters (Apt-CuNCs) is carried out by using DNA as a template, Apt-CuNCs are used to recognize total AFP, the content of total AFP is determined by using the generated Cu electrochemical signal, then, the Apt-CuNCs are subjected to enzymatic cleavage and stripping by using exonuclease I, alpha-fetoprotein heterogeneity (AFP-L3) is labeled by using lentil agglutinin (LCA) functionalized silver nanoparticles LCA@AgNPs, the content of AFP-L3 is determined by using the generated Ag electrochemical signal, and AFP-L3% can be calculated by combining the data of the two, and the electrochemical biosensor constructed by the method can be used for determining AFP-L3% in human serum.

The invention relates to the technical field of antibacterial materials, in particular to an antibacterial nano composite material and a preparation method thereof. The method comprises the following steps: mixing a cerium source solution and an organic ligand solution for reaction, and adding a buffer solution after the reaction is finished to obtain a cerium metal organic framework; mixing the cerium metal organic framework and vancomycin, and reacting to obtain a composite material; a substrate is soaked in a solution containing a coupling agent and a composite material, and the antibacterial nano composite material is formed on the surface of the substrate through deposition. Through the synergistic effect of the cerium metal organic framework and vancomycin, common pathogenic bacteria can be efficiently inhibited. As a carrier, the organic framework can realize slow release of vancomycin, so that the antibacterial action time is prolonged, and the antibacterial effect reduction caused by too fast release of vancomycin in a short time is also avoided. In addition, the antibacterial nano composite material has good biological safety and corrosion resistance.

The invention relates to the technical field of crossing of bone tissue engineering and intelligent antibacterial materials, in particular to a heterojunction nanocomposite and a preparation method thereof, and a heterojunction stent and a preparation method and application thereof. The preparation method comprises the following steps: by taking pCu-BTC and MoS2 as raw materials, carrying out ball milling in a dispersion medium at room temperature, then carrying out intermittent ultrasonic treatment under an ice bath condition to form a type II heterojunction interface so as to obtain a heterojunction nano composite material, compounding the heterojunction nano composite material with poly-L-lactic acid, and then carrying out high-pressure homogenization-3D printing forming, thereby obtaining the ultrasonic response type MoS2 / pCu-BTC / PLLA heterojunction scaffold. The stent has the sonodynamic / chemical dynamic dual-mode antibacterial and osteogenesis promoting functions, is good in biocompatibility and controllable in degradation, can be widely applied to the field of infectious bone defect repair, and provides a novel material solution for antibacterial-bone regeneration synergistic treatment.

The invention relates to a drug-eluting nanoengineered medical implant / contact device. The device comprises a nanocomposite and a drug, wherein the nanocomposite comprises a hydrophilic polymer domain, a hydrophobic polymer domain, a water pore, and a boundary charged double layer; wherein when the drug is hydrophilic, at least 80% of the drug partition in the boundary charged double layer is formed at the boundary interface of the hydrophilic polymer domain and the water pore, and when the drug is hydrophobic, at least 80% of the drug partition in the boundary charged double layer is formed at the boundary interface of the hydrophobic polymer domain and the water pore. The device is configured to maintain release of the drug with high precision and long duration.

The invention relates to the technical field of preparation of antitumor drugs, in particular to a drug delivery system based on mesoporous polydopamine and a preparation method and application thereof. The mesoporous polydopamine and the substrate are used for loading hydrophobic simvastatin, cholesterol oxidase and manganese acetate at the same time, and the nano composite material coated with hyaluronic acid has good drug loading capacity, realizes targeted release of drugs, and kills tumor cells and inhibits proliferation of the tumor cells through consumption of the drugs on tumor cell cholesterol.

The present invention relates to antimicrobial compositions comprising graphene-silver cation nanocomposites and uses for same as a broad-spectrum antimicrobial agent, and uses for same for treating microbial infections, including infections by antimicrobial resistant and / or multidrug resistant pathogens.

The present invention relates to a drug-eluting nanoengineered medical implant / contact device. The device comprises a nanocomposite and a drug, wherein the nanocomposite comprises hydrophilic polymer domains, hydrophobic polymer domains, water pores, and boundary charged double layers; wherein when the drug is hydrophilic, at least 80% of the drug partitions in the boundary charged double layers formed at the boundary interface of the hydrophilic polymer domains and water pores, and when the drug is hydrophobic, at least 80% of the dmg partitions in the boundary charged double layers formed at the boundary interface of the hydrophobic polymer domains and water pores. The device is configured to sustain the release of the drug at high precision and long duration.

The invention discloses a preparation method of novel medical polymethyl methacrylate bone cement, and relates to the field of medical materials. Comprising the following steps: (1) dissolving PMMA powder in dimethyl sulfoxide to obtain a PMMA solution; and (2) adding the MoS2 nanosheets into the PMMA solution, stirring the obtained mixture at 78-82 DEG C for 22-26 hours, and after the stirring process is finished, drying the mixture to obtain the MoS2-PMMA nano composite material. And (3) uniformly mixing the MoS2-PMMA nano composite material with MMA liquid, filling the obtained mixture into a mold, and carrying out curing molding, so as to obtain the novel medical polymethyl methacrylate bone cement. According to the invention, the molybdenum disulfide nanosheet is used as a modified substance, and the prepared MoS2-PMMA nano composite material has obviously improved mechanical properties, osteogenic activity and antibacterial properties.

The invention belongs to the technical field of biomedicine, and particularly relates to a diagnosis and treatment integrated nano composite material for targeting tumor blood vessels as well as a preparation method and application of the nano composite material. The invention aims to provide the diagnosis and treatment integrated nano composite material for targeting tumor blood vessels, so that organic integration of multi-mode molecular imaging and targeted therapy is successfully realized, an innovative solution is provided for constructing a novel diagnosis and treatment integrated platform, the technical blank of existing blood vessel targeting drugs is filled, and the application prospect is wide. And meanwhile, the treatment and monitoring functions are innovatively integrated to meet the urgent clinical requirements. According to the present invention, based on the precise evaluation requirement of tumor blood vessel targeting treatment, the MRI imaging agent and the blood vessel targeting drug are grafted and integrated on the POSS through the chemical bond for the first time, such that the treatment-monitoring integration is achieved, the targeting property and the synergistic treatment effect of the nanoparticles are systematically evaluated, the pain point that the action process cannot be evaluated in real time by using the traditional drug is solved, and the application prospect is broad. And a basis is provided for clinical individualized treatment.

The present invention discloses a kind of bacterial vesicle-coated core-shell structure ZnO2-Fe3O4 nanocomposite material, preparation method and application thereof. The core-shell structure ZnO2-Fe3O4 nanocomposite material disclosed by the present invention is ZnO2-Fe3O4@MVs, which is composed of Streptococcus gordonii (S.gordonii) vesicle-coated ZnO2-Fe3O4 nanoparticles; the ZnO2-Fe3O4 nanoparticles are assembled by Fe3O4 nanoparticles with surface modification of 3,4-dihydroxyphenylpropionic acid and ZnO2 nanoparticles with surface modification of polyvinylpyrrolidone. It has homologous targeting to enhance the effect of selective uptake for Streptococcus gordonii (S.gordonii), and preferentially removes Streptococcus gordonii (S.gordonii), a basal bacteria of the symbiotic biofilm in the oral cavity, thereby causing the structural collapse of the symbiotic biofilm, thereby removing the main pathogenic bacteria of periodontitis (Porphyromonas gingivalis), providing a new strategy for treating periodontitis.

The invention discloses a method, a system and a device for screening a conductive network structure of a polymer composite, and relates to the technical field of conductive polymer nano composites.The method comprises the steps that molecular dynamics simulation trajectory data of a polymer composite system of carbon nano tubes with different concentrations is obtained, particle nodes are sequentially coded, and the particle nodes are obtained; carrying out one-hot coding according to particle types and molecule IDs to which the particles belong, generating a node attribute matrix, and meanwhile, generating an adjacent matrix according to a bonding relationship among the particles so as to construct a corresponding graph structure data set; inputting the graph structure data set into a prediction model based on a graph attention network, and training by adopting an incremental learning method; according to the trained prediction model, the optimal polymer composite conductive network structure is screened out, and the method can capture complex multilevel structure information and better predict the polymer properties affected by the complex multilevel structure information.

A system for the synthesis and production of a cermolybdate graphitic carbon nitride (Ce2 (Mo4)3@g-C3N4) nanocomposite for the detection of uric acid in sweat, comprising: a) a cermolybdate synthesis unit for the production of Ce2 (Mo4)3 nanoparticles by hydrothermal treatment, wherein the cermolybdate synthesis unit comprises: • a reaction vessel containing cerium nitrate and ammonium molybdate in a molar ratio of 2:3 in a solvent mixture of ethanol and deionized water, • a surfactant additive system configured to add cetyltrimethylammonium bromide (CTAB) for stabilization, and • a hydrothermal reactor configured to heat the mixture to 180°C for 24 hours; b) a plant for the synthesis of graphitic carbon nitride, configured to produce g-C3N4 by thermal polymerization of melamine under inert atmospheric conditions at temperatures of 400 °C to 600 °C; and c) a nanocomposite formation unit configured to combine Ce2(Mo4)3 and g-C3N4 to form the Ce2(Mo4)3@g-C3N4 nanocomposite, wherein the nanocomposite formation unit comprises: • an ultrasound system configured to disperse the components at the molecular level and allow them to interact with each other, and • a drying system for removing residual solvents.

The application discloses a cell membrane modified nanocomposite as well as a preparation method and application thereof. The nanocomposite comprises, from outside to inside, a membrane structure outer layer, a piezoelectric material middle layer and a magnetostrictive material core structure, wherein the outer surface of the membrane structure contains protein molecules. Under the stimulation of an external magnetic field, the nanocomposite can regulate Th1 / Th2 balance, promote Th2 cell polarization and enhance T cell proliferation, thereby reducing T cell-mediated autoimmune reactions and maintaining host immune homeostasis, and has a wide application prospect in the treatment of arthritis.

This application relates to the field of cell preservation, and discloses a long-term preservation medium for stem cells based on a three-dimensional biomimetic microenvironment, which comprises the following component materials in parts by mass: flexible skeleton, benzoyl peroxide, superoxide dismutase, retinoic acid, ferulic acid, mixed buffer, nucleic acid aptamer targeting stem cells, serum-free medium, bFGF and TGF-β. Its construction method comprises the following steps: S1, three-dimensional scaffold construction; S2, antioxidant loading; S3, buffer preparation; S4, aptamer modification; S5, medium integration. The three-dimensional scaffold structure constructed by natural polysaccharides and nanocomposites, combined with the biomimetic pore and flexible network design, effectively simulates the extracellular matrix microenvironment. At the same time, by improving the structural stability and chemical compatibility of the cell adhesion interface, the cell morphology maintenance and adhesion force maintenance during the freezing process are realized, so that the scaffold provides a dynamically adaptable stable environment for cells structurally.

The application provides cytosine-rich ssDNA (C-rich ssDNA) and application thereof in improving peroxidase-like activity of Fe3O4@Au, and belongs to the technical field of nanocomposites; the C-rich ssDNA is obtained by connecting 20 continuous cytosine nucleotides to an aptamer; the sequence of the 20 continuous cytosine nucleotides is shown in SEQ ID NO. 1. Fe3O4@Au shows peroxidase-like activity, and after the C-rich ssDNA is used to modify Fe3O4@Au, the peroxidase-like activity is significantly enhanced, and then the detection limit of a sensing method based on Fe3O4@Au is reduced.

An electrochemical immunosensor device for the early detection of Orientia tsutsugamushi-specific TSA antigens, consisting of a carbon screen-printed paper electrode (SPPE) modified with AuNPs@rGO nanocomposites, an antibody immobilization interface and an electrochemical detection circuit.

The present invention discloses an active maintenance process for cryopreserving autologous bone at deep low temperature, belonging to the technical field of autologous bone preservation. The raw materials by weight include: 1-2 parts of L-proline, 5-8 parts of PVP / WS2 nanocomposite, 0.1-0.5 part of matrix metalloproteinase (MMPs) inhibitor, 1-2 parts of antifreeze protein (AFPs), 1-2 parts of non-permeating protective agent, 1-2 parts of trimethylammonium lauryl chloride, and 2-3 parts of oxygen carrier. This autologous bone cryopreservation medium can reduce the use of the traditional cryoprotectant DMSO, reduce its toxicity to cells and tissues, effectively slow down the damage of ice crystals to cells during long-term deep low temperature cryopreservation, maintain the integrity of collagen fibers in bone tissue, and inhibit the decrease in the activity of growth factors.

A biodegradable and biocompatible barrier membrane of piezoelectric nano composites of Metallic Oxide (MO) (e.g., Magnesium oxide, Zinc oxide and iron oxide)-PLLA (Poly-L-lactide), which can be subjected to acoustic pressure from ultrasound, to generate useful electrical charge for enhanced bone regeneration and enhanced antibacterial effects for guided bone regeneration to treat dental diseases, such as periodontitis.

The application discloses a lung-targeted nanocomposite and a preparation method and application thereof, wherein the nanocomposite comprises carbon nitride two-dimensional nanosheets and AIE photosensitizers loaded on surfaces of the carbon nitride two-dimensional nanosheets. The nanocomposite has excellent aggregation-induced emission properties and active oxygen generation capacity, can play a diagnostic role of the photosensitizer, and can produce a better tumor elimination effect, so that the diagnosis and treatment of lung cancer can be simultaneously realized. In addition, the nanocomposite has a suitable size, can enhance the retention of drugs in the lung, prolong the treatment time, and reduce the administration frequency; the nanocomposite can also be administered through tracheal spray, has the advantages of rapid onset, high local drug concentration, small drug dosage, convenient use, less systemic adverse reactions, easy monitoring and adjustment of the dosage, and the like.

The present invention relates to: a cerium oxide nanocomposite in which a shell layer comprising a cationic amino acid and PVP is formed on a core layer of cerium oxide nanoparticles; and a composition comprising same as an active ingredient for improving skin conditions. Employing lysine, which is an optimal amino acid capable of functioning as a multifunctional ligand among various amino acids, the nanocomposite of the present invention exhibits both excellent particle properties and maximized reactive oxygen species scavenging activity within skin tissue. Therefore, it can reduce and alleviate various skin tissue damages caused by oxidative stress including skin aging.