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Pleurotus geesteranus strain Qian Xiu No.3 and application thereof

A technology of Xiuzhen mushrooms and strains, applied in the application, fungi, mushroom cultivation and other directions, can solve the problems of few Xiuzhen mushroom varieties and long production cycle, and achieve the effects of short cultivation cycle, simple detection method and fast speed.

Active Publication Date: 2022-03-08
GUIZHOU INST OF SOIL & FERTILIZER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems that there are few varieties of chrysanthemum chrysanthemum in current production, and the production cycle is long, the purpose of the present invention is to provide a kind of chrysanthemum chrysanthemum strain

Method used

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  • Pleurotus geesteranus strain Qian Xiu No.3 and application thereof
  • Pleurotus geesteranus strain Qian Xiu No.3 and application thereof
  • Pleurotus geesteranus strain Qian Xiu No.3 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 The collection, separation and domestication process of bacterial strain Qianxiu No. 3 of the present invention

[0041] In August 2019, the inventor discovered and collected a wild fruiting body similar to Pleurotus osmanthus in the Aha Lake Reservoir area of ​​Huaxi District, Guiyang City, Guizhou Province. The sporocarp is separated from tissue to obtain mycelia (bacteria); the bacterial strain obtained after tissue separation is subjected to a cultivation fruiting test; then the sporocarp with a good flower shape is separated from the tissue, inoculated on a PDA medium for cultivation, The strains with fast mycelial growth and uniform growth were screened and continued to be cultivated and domesticated. After several generations of cultivation, a strain with stable genetic properties and short fruiting cycle was screened out, named: Qianxiu No. 3.

Embodiment 2

[0042] The taxonomic identification of embodiment 2 bacterial strain of the present invention Qianxiu No. 3

[0043] (1) Morphological identification of Qianxiu No. 3 strain:

[0044] The fruiting body is clustered, the cap is light brown and fan-shaped, the cross section of the cap is flat, the middle part is slightly concave, the diameter of the cap is 4.3-10.2cm, an average of 7.5cm; the thickness of the cap is moderate, 0.7-1.1cm, an average of 0.8cm; the length of the stipe is 0.9 ~2.1cm; gills without reticulation, gills extending to the top of the stipe; fruiting bodies dense in texture. According to the photos and corresponding feature descriptions on page 990 of "China's Large-scale Fungal Resources Illustrated Book" (edited by Li Yu, Zhongyuan Farmers Publishing House, published in 2015), Qianxiu No. 3 and Xiuzhen mushroom (Pleurotus lungus) The characteristics are consistent, and it is preliminarily determined that Qianxiu 3 belongs to Pleurotus pulmonarius in the cl...

Embodiment 3

[0050] Embodiment 3 The PAPD comparative analysis test of bacterial strain Qianxiu No. 3 of the present invention and other pear mushroom bacterial classifications is carried out according to the following method:

[0051] Plant Genome DNA Extraction Kit CW0531 (Beijing Kangwei Century Company) was used to extract Qianxiu No. 3, Pteris cerevisiae (light gray Xiuzhen mushroom selected from Beijing Jiji Garden), Jingxiu No. 1 (selected from Beijing Jijiyuan) 20 RAPD primers were selected from the genome DNA of strains bred from light brown Pleurotus genii), Xiu 03 (the gray Pleurotus edodes in Taijiang, Qiandongnan Chuangjian), and Xiaxiu 004 (brown Pleurotus edodes bred by Jiangsu Tianlong Fungus Industry) strains (See Table 1) for PCR amplification.

[0052] Table 1 RAPD random primer sequence

[0053]

[0054]

[0055] The RAPD-PCR amplification reaction system is: total volume 20 μL: 20-50ng / μL DNA template 1 μL, 10×Buffer (with Mg 2+ ) 2μL, 2.5mM dNTP 0.5μL, 5U / μl E...

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Abstract

The invention belongs to the field of edible mushrooms, and particularly discloses a pleurotus geesteranus strain Qian Xiu No.3, and the preservation number of the pleurotus geesteranus strain Qian Xiu No.3 is CCTCC (China Center for Type Culture Collection) NO: M20211210. The invention further discloses application of the strain in the aspects of food, health care products or beverages and the like. The pileus of the Qian Xiu No.3 strain is light brown, the flower shape is full, and the commodity value is high; secondly, the cultivation period is short, and compared with the production period of the existing pleurotus geesteranus main cultivated variety, the production period of the Qian Xiu No.3 is shortened by at least 15-20 days; in addition, the yield of the Qian Xiu No.3 is high, the average yield of each bag is 352.3 g, and the economic benefit is high. The invention also discloses a molecular marker with specificity to the strain, and when the molecular marker is used for identifying the Qianxiu No.3, the result is accurate and reliable; the detection method is simple and high in speed; and the method is suitable for on-site or real-time detection in authenticity or infringement identification of the Qian Xiu No.3.

Description

technical field [0001] The invention belongs to the field of edible fungi, and in particular relates to a strain of Pleurotus chinensis, and also relates to the application of the strain of Pleurotus pruriens. Background technique [0002] Xiuzhen mushroom (scientific name: Pleurotus pulmonarius. Alias: Pleurotus pleurotus, Pleurotus phoenix tail, Indian oyster mushroom or Himalayan oyster mushroom, etc.; its Latin name: Pleurotus pulmonarius) belongs to the species of Pleurotus genus Pleurotus in the family Pleurotus. Xiuzhen mushrooms are small and beautiful in appearance, not only tender in texture, delicious in taste, but also rich in nutrition, rich in protein, sugar, unsaturated fatty acids, vitamins, folic acid and various trace elements; the protein content of Xiuzhen mushrooms is higher than that of shiitake mushrooms and straw mushrooms. High, its fruiting body contains 18 kinds of amino acids, of which the content of aspartic acid and glutamic acid is relatively h...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N1/15C12Q1/6895C12Q1/686C12N15/11A01G18/00A01G18/20A61K36/07A61P37/04A23L31/00A23L33/10A23L2/52C12R1/645
CPCC12Q1/6895C12Q1/686A01G18/00A01G18/20A61K36/07A61P37/04A23L31/00A23L33/10A23L2/52A23V2002/00C12Q2565/125A23V2200/324A23V2250/208
Inventor 王晓敏张邦喜杜慕云陈旭朱森林杨珍陈波张钦语唐玲杨仁德
Owner GUIZHOU INST OF SOIL & FERTILIZER
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