47 results about "Immuno gold" patented technology

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an opposite second side; a conjugate pad or membrane disposed on said first side of said laminate; a sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said second side of said laminate. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay by dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an second side; a conjugate pad or membrane disposed on said first side of said laminate; a hinge region connecting sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; wherein said laminating material isolates the conjugate pad from the sample pad such as to slow the fluid path into the fibrous sample pad, and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said first side of said laminate. The more complete mixing permitted by this temporary obstruction provides an important feature of the invention that gives this 2-step test its superior performance. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

The invention relates to a primer pair for rapidly detecting silkworm microsporidia and a reagent kit and a detection method thereof. The nucleotide sequence of the primer pair is shown as SEQ ID NO.7 and SEQ ID NO.8. By the utilization of the primer pair, different nucleic acid molecular markers are added into the upstream primer and the downstream primer respectively, PCR products of the marked primers can be directly determined through immuno-gold test strips, the operation steps are simplified, convenience is achieved, specificity is good, and sensitivity is high. Compared with a traditional gel electrophoresis method, the sensitiveness is increased by 10 times, and the primer pair can serve as an ideal detection means for silkworm microsporidia in silkworm finished product eggs and has good application prospects.

A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay by dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

The invention discloses a preparation method of a rapid nucleic acid detection kit for detecting canis familiaris components in feed and an application method thereof, and belongs to the fields of molecular biology and immunology. According to the invention, a high sensitivity and high specificity method of polymerase chain reaction in nucleic acid detection and an immuno gold staining rapid detection technology in an immunological detection are combined, a specific primer is designed and labeled, the extracted target DNA is subjected to specific amplification, and amplified product is combined with a gold labeled antibody immobilized on a test paper tape, so as to form a stable and visible detection band and quality control band, thereby achieving the rapid and correct detection of the canis familiaris components in the main feed.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention discloses a preparation method of immunogold rapid test paper for vibrio parahaemolyticus. The method comprises the following steps of: (a) preparing a vibrio parahaemolyticus antigen; (b) preparing a vibrio parahaemolyticus inactivated vaccine; (c) preparing a polyvalent antiserum with a vaccine immune experimental rabbit; (d) purifying the antiserum; (e) marking with colloidal gold; and (f) preparing an immunogold test strip. A detection technology in which the immunogold rapid test paper for vibrio parahaemolyticus is adopted has the advantages of easiness, sensitivity, rapidness, specificity and the like; the customs clearance speed of port imported and exported goods can be increased greatly; and meanwhile, the test paper has the advantages of low price, no need of instrument, easiness and rapidness for operationing and easiness in judging results.

The invention provides a pair of hybridoma cell strains. The hybridoma cell strains comprise the first hybridoma cell strain and the second hybridoma cell strain which are stored independently. The preservation number of the first hybridoma cell strain is CGMCC NO.10495. The preservation number of the second hybridoma cell strain is CGMCC NO.10494. The invention further provides a pair of paired monoclonal antibodies excreted by the two hybridoma cell strains, an application of the paired monoclonal antibodies in detecting G2-EPSPS protein and immuno gold staining test paper. According to the technical scheme, the sensitivity of 1 ng / mL can be obtained through detection of the immuno gold staining test paper on G2-EPSPS protein, no cross reaction is carried out on CP4-EPSPS protein, no cross reaction is carried out on 27 non-GMO crops and 14 G2-EPSPS transgenic crops with different sources, and high sensitivity and specificity are achieved.

The invention relates to a colloidal gold test paper strip for carrying out the quick detection of the O-shaped foot and mouth disease antibody level of artiodactyls, the preparing method of the test paper strip and a specific detection method. A sample application absorption cushion made of porous fiber material, an immune gold label layer which is arranged on the tail end of the sample application absorption cushion and is connected with the sample application absorption cushion, a detection section made of nitrocellulose film, and an absorption section which is made of water absorption material and is arranged on the tail end of the detection section and is connected with the detection section are fixed on the base plate of the test paper strip. A quality control line is an O-shaped foot and mouth disease resistant rabbit or guineapig IgG antibody, a detection line is coated with O-shaped foot and mouth disease virus, and the O-shaped foot and mouth disease virus of the detection line of the detection section and the colloidal gold label attached to the immune gold label layer is natural virus, or DNA recombination expression foot and mouth disease virus, or the decomposed antigen of the foot and mouth disease.

A kit for dot immunogold directed filtration assay including a dot immunogold directed filtration card, a detection probe labeled by nano colloidal gold or latex beads, a negative standard, a positive standard, and a cleaning solution.

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an opposite second side; a conjugate pad or membrane disposed on said first side of said laminate; a sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said second side of said laminate. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

The invention discloses a method for detecting a foot and mouth disease (FMD) non-structural protein 3AB antibody by using dot immuno-gold filtration assay (DIGFA). The method specifically comprises the following steps of: a) synthesizing a foot and mouth disease(FMD) non-structural protein 3AB; b) preparing SPA colloidal gold; c) manufacturing a reaction kit; and d) applying the reaction kit as a carrier, placing antigen sites of the 3AB non-structural protein synthesized in the step a) on an NC membrane, sealing the NC membrane and adding a sample to be detected, and after washing the NC membrane, using the SPA prepared in the step b) as the colloidal gold to mark the protein for detecting the FMD non-structural protein 3AB antibody. The method has the characteristics of simple, convenient and fast operation, no need of special instrument, bright spot color, strong specificity, high sensitivity, easy judgment of results and preservable detected samples.

The invention discloses a nucleic acid rapid detection kit for a geese origin component in food and feed and an application method of the kit, belonging to the fields of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method of PCR in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, extracted target DNA is subjected to specific amplification through designing a specific primer and labeling the primer and the amplified product is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form stable and visible detection zone and quality control zone, thereby realizing the rapid and accurate detection of geese origin component in food and feed.

The invention discloses a composition for detecting a rheumatoid arthritis immune antibody by a dot immuno-gold filtration assay, comprising CCP (Casein Calcium Peptide), a high polymer, a microfiltration membrane, a water-absorbing medium and a vector medium, wherein the CCP is covalently combined with the high polymer; and then the high polymer is combined to the microfiltration membrane in a covalence or covalent mode. The composition not only can realize the quick in vitro detection of the rheumatoid arthritis immune antibody, but also is convenient for self-help diagnosis of a patient and provides the reference to knowing the development of disease condition in time.

The invention discloses a detection method of a new coronavirus SARS-CoV-2 antigen, and belongs to the technical field of biology. The method comprises the following steps of capturing nanoparticles by virtue of new coronavirus SARS-CoV-2S protein immunomagnetic beads, carrying out specific immunization on the nanoparticles and S protein, and incubating with immune gold signal nanoparticles so as to construct a sandwich structure; detecting an SERS signal through a Raman spectrometer, specifically detecting the new coronavirus SARS-CoV-2 protein within 5 minutes, and specifically detecting the pseudovirus and variant pseudovirus of the SARS-CoV-2 and the pseudovirus diluted in human saliva. Through the above mode, nucleic acid extraction or antibody-dependent detection is not needed, meanwhile, the method has the advantages of being good in stability, higher in specificity and affinity, low in immunogenicity, easy to chemically modify, simple and convenient to operate and the like, the rapid detection of the new coronavirus SARS-CoV-2 antigen and the variant virus can be achieved within five minutes, and a condition is created for the early detection of the 2019SARS-CoV-2 pathogen.

A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay. By dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

The invention discloses a kit for detecting immuno-gold synchronous scattering spectrum of human serum complement 3 and a use method thereof. The kit comprises three reagents, wherein the reagent 1 is a calibrator containing the frozen dry plasma protein reference serum of the complement 3, the reagent 2 contains 111.5-164.5mM of Na2HPO4, 17.5-45.0mM of citric acid solution and 50-70g / ml of PEG6000, and the reagent 3 contains gold-labeled goat anti-human C3 antibody and 0.03-0.06 g / L of PEG20000. The use method comprises the following steps: firstly preparing a C3 standard series, adding the reagent 1, reagent 2 and reagent 3 according to a defined ratio, performing constant volume process, incubating for 15min in a ultrasonic reactor, scanning the synchronous scattering spectrum with a fluorescence spectrophotometer to detect the synchronous scattering value, and calculating the C3 content according to the standard curve. The kit of the invention can be used to accurately and qualitatively detect the complement C3, be suitable for the clinical and scientific research analysis of complement 3 in samples such as serum and plasma, and have the advantages of convenient, fast and sensitive operation, low detection limit, wide linear range, simple phase separation process and low sample consumption.

A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay. By dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

The invention discloses a preparation method of an electrochemical sensing element based on immunogold. A common nitroimidazole type structure hapten is prepared, and the nitroimidazole hapten is coupled with carrier proteins (BSA and OVA) by adopting an active ester method to prepare an immunogen and a coating antigen, wherein the mol ratio of coupling molecules is (11 to 1) and (10 to 1). A nitroimidazole antibody is simultaneously prepared and the valence is (1 to 100000), and immune nano gold is prepared; and a monomolecular film is formed on a gold electrode by using a monomolecular film self-assembling technology, and is connected with the immune nano gold through DCC and NHS to prepare the sensing element. The sensing element has the good responses on metronidazole, tinidazole and dimetridazole; the cross reaction rate is 90% or more; metronidazole, tinidazole and dimetridazole and metabolites of metronidazole, tinidazole and dimetridazole in a concentration range of 0.001-100mg / L can be determined; and the detection limit is about 0.0019mg / L and the recycling rate is about 90%.

A reagent kit using flow closing technique for immuno-gold label detection features that after the specimen to be tested is added to the specimen hole on detection board or the test strip is insertedin the specimen bath, the chromatographic moving of specimen drives the closing agent of specimen pad or colloidal gold pad to nitrocellulose film, so performing seal in flow procedure. Its advantages include simplified process, low cost, no damage to nitrocellulose film and high sensitivity.

The invention relates to a method for labeling a typhoid salmonella recombinant antigen by colloidal gold. The method comprises the following steps: (1) preparing a colloidal gold solution; (2) adjusting the pH value of the prepared colloidal gold solution to be lower than the optimal pH value by 1.5-2.0; (3) adding the labelled typhoid salmonella recombinant antigen at one time, wherein the adding amount is 70%-80% lower than the optimal use amount of the labelled typhoid salmonella recombinant antigen; and (4) adding a sealing agent and a stabilizing agent, and finishing labeling. The safety range is broken, the color development intensity and sensitivity of the product can be obviously improved by placing immune gold under a relatively unstable and controllable condition, the 'relatively unstable and controllable condition' means that the pH value labelled by colloidal gold is slightly lower than the isoelectric point labelled by typhoid salmonella recombinant antigen, and the use amount of the labelled typhoid salmonella recombinant antigen is unsaturated; and the sealing agent and the stabilizer are added at the same time, so that the color development intensity of the product is obviously deepened, and the sensitivity is obviously improved.

The invention discloses a comprehensive buffer solution for detecting brucellosis by means of a colloidal immuno-gold immuno-filtration assay. The comprehensive buffer solution is characterized by comprising a hypotonic buffer solution, a non-ionic surfactant, an ampholytic surfactant, univalent salt ions, a metal ion chelating agent and inert occludin which is negatively charged in the hypotonic buffer solution. Compared with the prior art, the comprehensive buffer solution has the advantages of being capable of detecting a whole-blood sample, convenient to operate, accurate in result and the like.

The invention provides a protein immunogold labeling method for a cryoelectron microscope. The protein immunogold labeling method comprises the following steps of (1) respectively pretreating a grid of the frozen electron microscope by utilizing a poly-L-lysine solution and PBS (Phosphate Buffer Solution); (2) putting the grid in a culture dish with the front surface facing upwards, dripping a sample onto the grid, standing, incubating, and eluting with PBS (Phosphate Buffer Solution); 3) adding a sealing agent into the culture dish, standing, incubating, and eluting with PBS; 4) adding a primary antibody, namely a specific antibody of the target protein, into the culture dish, standing, incubating, and eluting with PBS; 5) adding a secondary antibody, namely an immune gold antibody selected according to the species of the primary antibody, into the culture dish, standing, incubating, and eluting with PBS; and 6) taking out the grid, imaging by using a freezing electron microscope, andanalyzing image data to obtain a target protein structure. The method is advantaged in that an immunolabelled protein technology and a freezing electron microscope tomography technology are combined,so the target protein is observed in the freezing electron microscope imaging, and the structure is analyzed at the same time.

The invention discloses a rapid nucleic acid test strip detection kit of food-borne pathogenic microorganism Bibrio parahemolyticus and an application method of the kit, belonging to the technical field of bacteria classification inspection. According to the invention, a high-sensitivity high-specificity method of PCR reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, specific amplification is carried out on target DNA by using a labeled specific primer through design of a specific primer, the amplified primer is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form a stable and visible detection zone and a quality control zone, thereby realizing the rapid and accurate detection of the Bibrio parahemolyticus.

The invention relates to a dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and a preparation method thereof. The detection card is composed of a sample layer, a colloidal gold labeled layer, a detection layer, a water absorption layer, a plastic lining plate and a plastic cover plate. The preparation method comprises the following steps: preparing a colloidal gold labeled leather hydrolyzed protein solution and a colloidal gold labeled layer; preparing a leather hydrolyzed protein multi-clone antibody solution and detection lines on the detection layer; and assembling the detection card. The detection card has the advantages of simple operation and accurate detection result, can be interpreted by naked eyes, does not need complex operation, special equipment and the like, is suitable for on-site casual detection of leather hydrolyzate in milk for milk product enterprises, and inspection and sanitary departments, and has wide application prospects.

The invention discloses an immuno-gold labeling card for the general detection of fluoroquinolones and a preparation method thereof. The immuno-gold labeling card comprises a colloidal gold pad and a chromatography membrane. The colloidal gold pad is sprayed with a monoclonal antibody protein generated by a hybridoma cell strain, wherein the hybridoma cell strain is labeled by colloidal gold and the preservation number of the hybridoma cell strain is CCTCC NO.C2015221. A detection line on the chromatography membrane is sprayed with a conjugate of enrofloxacin and cationized bovine serum albumin. A control line on the chromatography membrane is sprayed with a Goat Anti-Mouse secondary antibody IgG(H+L). According to the invention, a monoclonal antibody, which is high in sensitivity and strong in universality for ciprofloxacin, enrofloxacin, norfloxacin, pefloxacin, enoxacin and the like when measured through the indirect competition ELISA assay, is adopted. After that, the monoclonal antibody is applied to the immuno-gold labeling card for the detection of fluoroquinolones. Therefore, the prepared immuno-gold labeling card is still high in sensitivity and strong in universality for target drugs. In this way, the immuno-gold labeling card can be used for the rapid and qualitative screening of mainly fluoroquinolones in various fields.

The invention discloses a hybridoma cell strain which can stably secrete a high-titer anti-human HFABP (heart fatty acid binding protein). The Preservation No. of the hybridoma cell strain is CCTCC NO: C2014196; and by taking a purified recombinant expressed HFABP as an antigen for immunizing a female BALB / c mouse, lymphocytes of the immunized mouse are fused with sp2 / 0 cells, and through indirect ELISA (enzyme-linked immuno sorbent assay) and Western blotting detection, the titers respectively reach 1:104 and 1:4000. A monoclonal antibody secreted by the cell strain has good specificity and relative affinity, can be used as a key raw material for the early detection of HFABP, and provides an important raw material for the establishment of a clinical diagnostic reagent which is applied to the rapid and high-sensitivity detection of HFABP and comprises an immune-gold label, an immune test strip, an enzyme-linked immunoreagent, immunoturbidimetry, and the like; and the monoclonal antibody can also be used as an important raw material of a diagnostic reagent which is used for detecting the HFABP of the cerebrospinal fluid, and monitoring and predicting the transformation, from mild cognitive impairment to paraphrenia, of old people.

The invention discloses an antibody dispersing agent for homogeneous labeling of immuno gold staining and application of the antibody dispersing agent, and belongs to the technical field of immuno gold staining. The antibody dispersing agent for homogeneous labeling of immuno gold staining comprises the following components in percentage by mass of 0-30% of bovine serum albumin, 0-30% of polyvinylpyrrolidone, 0-30% of polyethylene glycol and the balance of solvent. The antibody dispersing agent can be applied to an immune colloidal gold homogeneous labeled antibody. According to the technicalscheme, the consistency of the labeling effect can be remarkably improved, and the feeding amount of the to-be-labeled sample is reduced.