44 results about "Immuno gold" patented technology

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an second side; a conjugate pad or membrane disposed on said first side of said laminate; a hinge region connecting sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; wherein said laminating material isolates the conjugate pad from the sample pad such as to slow the fluid path into the fibrous sample pad, and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said first side of said laminate. The more complete mixing permitted by this temporary obstruction provides an important feature of the invention that gives this 2-step test its superior performance. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

The invention relates to a primer pair for rapidly detecting silkworm microsporidia and a reagent kit and a detection method thereof. The nucleotide sequence of the primer pair is shown as SEQ ID NO.7 and SEQ ID NO.8. By the utilization of the primer pair, different nucleic acid molecular markers are added into the upstream primer and the downstream primer respectively, PCR products of the marked primers can be directly determined through immuno-gold test strips, the operation steps are simplified, convenience is achieved, specificity is good, and sensitivity is high. Compared with a traditional gel electrophoresis method, the sensitiveness is increased by 10 times, and the primer pair can serve as an ideal detection means for silkworm microsporidia in silkworm finished product eggs and has good application prospects.

A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay by dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

The invention discloses a preparation method and an application method of a nucleic acid rapid detection kit for Bostaurus components in feeds and belongs to the field of molecular biology and immunology. According to the preparation method and the application method, a high-sensitivity and high-specificity method of a polymerase chain reaction in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunology detection; a unique primer is designed and the primer is labeled; an extracted target DNA is subjected to specific amplification and an amplified product is combined with a labeled antibody immobilized on a test strip in a developing solution to form a stable and visible detection strip and a quality control strip, so as to realize rapid and accurate detection on the bovine-derived components in food and feed.

The invention provides a pair of hybridoma cell strains. The hybridoma cell strains comprise the first hybridoma cell strain and the second hybridoma cell strain which are stored independently. The preservation number of the first hybridoma cell strain is CGMCC NO.10495. The preservation number of the second hybridoma cell strain is CGMCC NO.10494. The invention further provides a pair of paired monoclonal antibodies excreted by the two hybridoma cell strains, an application of the paired monoclonal antibodies in detecting G2-EPSPS protein and immuno gold staining test paper. According to the technical scheme, the sensitivity of 1 ng/mL can be obtained through detection of the immuno gold staining test paper on G2-EPSPS protein, no cross reaction is carried out on CP4-EPSPS protein, no cross reaction is carried out on 27 non-GMO crops and 14 G2-EPSPS transgenic crops with different sources, and high sensitivity and specificity are achieved.

A chromatographic lateral-flow assay system for rapid, high sensitivity method of detecting low levels of ligands in body fluids, with few false positives and few false negatives. The lateral-flow assay may have a membrane strip in ribbon form, which increases detection on the order of 2 to 10 fold over the conventional chromatographic specific binding assay techniques by placing a dried or lyophilized conjugate in colloidal spheres opposite side of the lateral flow membrane strip. A chromatographic specific binding assay strip device, comprising: a laminate strip having a first side and an opposite second side; a conjugate pad or membrane disposed on said first side of said laminate; a sample receiving pad or membrane strip and reservoir pad or membrane disposed on said second side of said laminate; and a detection pad or membrane strip disposed between the sample pad or membrane and the reservoir pad or membrane on said second side of said laminate. The assay system comprises a housing device, such as a test tube or cassettes to facilitate the mixing of a sample solution with the dried or lyophilized conjugate, and kits.

The invention discloses a method for detecting a foot and mouth disease (FMD) non-structural protein 3AB antibody by using dot immuno-gold filtration assay (DIGFA). The method specifically comprises the following steps of: a) synthesizing a foot and mouth disease(FMD) non-structural protein 3AB; b) preparing SPA colloidal gold; c) manufacturing a reaction kit; and d) applying the reaction kit as a carrier, placing antigen sites of the 3AB non-structural protein synthesized in the step a) on an NC membrane, sealing the NC membrane and adding a sample to be detected, and after washing the NC membrane, using the SPA prepared in the step b) as the colloidal gold to mark the protein for detecting the FMD non-structural protein 3AB antibody. The method has the characteristics of simple, convenient and fast operation, no need of special instrument, bright spot color, strong specificity, high sensitivity, easy judgment of results and preservable detected samples.

The invention discloses a nucleic acid rapid detection kit for a geese origin component in food and feed and an application method of the kit, belonging to the fields of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method of PCR in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, extracted target DNA is subjected to specific amplification through designing a specific primer and labeling the primer and the amplified product is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form stable and visible detection zone and quality control zone, thereby realizing the rapid and accurate detection of geese origin component in food and feed.

The invention discloses a composition for detecting a rheumatoid arthritis immune antibody by a dot immuno-gold filtration assay, comprising CCP (Casein Calcium Peptide), a high polymer, a microfiltration membrane, a water-absorbing medium and a vector medium, wherein the CCP is covalently combined with the high polymer; and then the high polymer is combined to the microfiltration membrane in a covalence or covalent mode. The composition not only can realize the quick in vitro detection of the rheumatoid arthritis immune antibody, but also is convenient for self-help diagnosis of a patient and provides the reference to knowing the development of disease condition in time.

The invention discloses a detection method of a new coronavirus SARS-CoV-2 antigen, and belongs to the technical field of biology. The method comprises the following steps of capturing nanoparticles by virtue of new coronavirus SARS-CoV-2S protein immunomagnetic beads, carrying out specific immunization on the nanoparticles and S protein, and incubating with immune gold signal nanoparticles so as to construct a sandwich structure; detecting an SERS signal through a Raman spectrometer, specifically detecting the new coronavirus SARS-CoV-2 protein within 5 minutes, and specifically detecting the pseudovirus and variant pseudovirus of the SARS-CoV-2 and the pseudovirus diluted in human saliva. Through the above mode, nucleic acid extraction or antibody-dependent detection is not needed, meanwhile, the method has the advantages of being good in stability, higher in specificity and affinity, low in immunogenicity, easy to chemically modify, simple and convenient to operate and the like, the rapid detection of the new coronavirus SARS-CoV-2 antigen and the variant virus can be achieved within five minutes, and a condition is created for the early detection of the 2019SARS-CoV-2 pathogen.

A chromatographic specific binding assay strip device, comprising: a non-permeable platform strip; a permeable membrane testing strip positioned on top of said non-permeable platform strip, with the testing strip comprising at least one capture reagent site containing a capture reagent for at least one specific analyte, a sample receiving pad positioned on top of and at a proximal end of the non-permeable platform strip, with the sample receiving pad having contact with a proximal end of said permeable membrane testing strip, a reservoir pad positioned on top of and at a distal end of said non-permeable membrane testing strip, with the reservoir pad having contact with a proximal end of said permeable membrane test strip; a supporting strip attached to and extending from the proximal end of said non-permeable platform strip; and a conjugate pad positioned on said supporting strip, said conjugate pad comprising a semi-permeable membrane containing a colorant conjugate. The semi-permeable membrane acts as a barrier between the conjugate pad and the sample receiving pad, regulating the flow through the semi-permeable membrane and overall flow of the assay. By dipping the conjugate pad into a sample solution, there will be increased binding between the analyte in the sample and the conjugate (preferably colloidal gold), thereby giving improved results on the lateral flow assay.

The invention relates to a method for labeling a typhoid salmonella recombinant antigen by colloidal gold. The method comprises the following steps: (1) preparing a colloidal gold solution; (2) adjusting the pH value of the prepared colloidal gold solution to be lower than the optimal pH value by 1.5-2.0; (3) adding the labelled typhoid salmonella recombinant antigen at one time, wherein the adding amount is 70%-80% lower than the optimal use amount of the labelled typhoid salmonella recombinant antigen; and (4) adding a sealing agent and a stabilizing agent, and finishing labeling. The safety range is broken, the color development intensity and sensitivity of the product can be obviously improved by placing immune gold under a relatively unstable and controllable condition, the 'relatively unstable and controllable condition' means that the pH value labelled by colloidal gold is slightly lower than the isoelectric point labelled by typhoid salmonella recombinant antigen, and the use amount of the labelled typhoid salmonella recombinant antigen is unsaturated; and the sealing agent and the stabilizer are added at the same time, so that the color development intensity of the product is obviously deepened, and the sensitivity is obviously improved.

The invention discloses a comprehensive buffer solution for detecting brucellosis by means of a colloidal immuno-gold immuno-filtration assay. The comprehensive buffer solution is characterized by comprising a hypotonic buffer solution, a non-ionic surfactant, an ampholytic surfactant, univalent salt ions, a metal ion chelating agent and inert occludin which is negatively charged in the hypotonic buffer solution. Compared with the prior art, the comprehensive buffer solution has the advantages of being capable of detecting a whole-blood sample, convenient to operate, accurate in result and the like.

The invention relates to a dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and a preparation method thereof. The detection card is composed of a sample layer, a colloidal gold labeled layer, a detection layer, a water absorption layer, a plastic lining plate and a plastic cover plate. The preparation method comprises the following steps: preparing a colloidal gold labeled leather hydrolyzed protein solution and a colloidal gold labeled layer; preparing a leather hydrolyzed protein multi-clone antibody solution and detection lines on the detection layer; and assembling the detection card. The detection card has the advantages of simple operation and accurate detection result, can be interpreted by naked eyes, does not need complex operation, special equipment and the like, is suitable for on-site casual detection of leather hydrolyzate in milk for milk product enterprises, and inspection and sanitary departments, and has wide application prospects.

The invention discloses an immuno-gold labeling card for the general detection of fluoroquinolones and a preparation method thereof. The immuno-gold labeling card comprises a colloidal gold pad and a chromatography membrane. The colloidal gold pad is sprayed with a monoclonal antibody protein generated by a hybridoma cell strain, wherein the hybridoma cell strain is labeled by colloidal gold and the preservation number of the hybridoma cell strain is CCTCC NO.C2015221. A detection line on the chromatography membrane is sprayed with a conjugate of enrofloxacin and cationized bovine serum albumin. A control line on the chromatography membrane is sprayed with a Goat Anti-Mouse secondary antibody IgG(H+L). According to the invention, a monoclonal antibody, which is high in sensitivity and strong in universality for ciprofloxacin, enrofloxacin, norfloxacin, pefloxacin, enoxacin and the like when measured through the indirect competition ELISA assay, is adopted. After that, the monoclonal antibody is applied to the immuno-gold labeling card for the detection of fluoroquinolones. Therefore, the prepared immuno-gold labeling card is still high in sensitivity and strong in universality for target drugs. In this way, the immuno-gold labeling card can be used for the rapid and qualitative screening of mainly fluoroquinolones in various fields.

The invention provides a hybridoma cell strain and application thereof. The hybridoma cell strain can secrete a monoclonal antibody of a tomato spotted wilt virus, and the monoclonal antibody can be applied to preparation of an immuno-gold label rapid-test card. A method comprises the following steps: 1. purifying a recombinant protein of the tomato spotted wilt virus; 2. immunizing a rabbit withthe purified recombinant protein of the tomato spotted wilt virus, so as to obtain a polyclonal antibody; 3. immunizing a mouse with the purified recombinant protein of the tomato spotted wilt virus,then, preparing the monoclonal antibody through cell fusion and cloning screening, finally, preparing ascitic fluid of the mouse, and carrying out purification, so as to obtain a tomato spotted wilt virus monoclonal antibody of the mouse; and 4. carrying out paired screening, so as to obtain paired monoclonal antibodies and polyclonal antibodies which are applied to the immuno-gold label rapid-test card. Therefore, the hybridoma cell strain has the advantage that the immuno-gold label rapid-test card can be applied to rapid, on-site and sensitive detection on the tomato spotted wilt virus.

The invention discloses a quantitative detection method for heavy metal cadmium ions, and belongs to the technical field of biological detection. The quantitative detection method comprises the following steps: S1, preparing a cadmium ion immunogold-labeled rapid detection card, specifically, wherein preparation of the cadmium ion immunogold-labeled rapid detection card comprises the following steps: (1), preparing a nitrocellulose membrane containing a detection line T and a quality control line C; (2) preparing a gold-labeled pad; (3) assembling an immunogold-labeled rapid test card; and S2, setting a standard curve for quantitative detection of a cadmium ion immunogold-labeled rapid detection card; S3, quantitatively detecting a sample by using the cadmium ion immunogold-labeled rapid detection card. According to the present invention, the immunogold labeling rapid detection card is prepared by using the gold labeling technology, combining the antigen-antibody immunoreaction and using the lateral chromatography principle, can be used for rapid immunodetection, and can be combined with the color generation analyzer, the color generation analyzer determines the depth of the C line and the T line, the T/C is calculated, and then linear fitting is carried out, and the corresponding parameters is set in the color generation analyzer, the color generation analyzer is applied, and a standard curve is set to realize quantitative detection.