Kit for detecting immuno-gold synchronous scattering spectrum of human serum complement 3 and use method thereof

Abstract

The invention discloses a kit for detecting immuno-gold synchronous scattering spectrum of human serum complement 3 and a use method thereof. The kit comprises three reagents, wherein the reagent 1 is a calibrator containing the frozen dry plasma protein reference serum of the complement 3, the reagent 2 contains 111.5-164.5mM of Na2HPO4, 17.5-45.0mM of citric acid solution and 50-70g / ml of PEG6000, and the reagent 3 contains gold-labeled goat anti-human C3 antibody and 0.03-0.06 g / L of PEG20000. The use method comprises the following steps: firstly preparing a C3 standard series, adding the reagent 1, reagent 2 and reagent 3 according to a defined ratio, performing constant volume process, incubating for 15min in a ultrasonic reactor, scanning the synchronous scattering spectrum with a fluorescence spectrophotometer to detect the synchronous scattering value, and calculating the C3 content according to the standard curve. The kit of the invention can be used to accurately and qualitatively detect the complement C3, be suitable for the clinical and scientific research analysis of complement 3 in samples such as serum and plasma, and have the advantages of convenient, fast and sensitive operation, low detection limit, wide linear range, simple phase separation process and low sample consumption.

Description

technical field [0001] The invention relates to a biochemical analysis technology, in particular to an immunogold synchronous scattering spectrometry kit for detecting human serum complement 3 and a use method thereof. Background technique [0002] The human complement 3 (complement component 3, referred to as C3) component is the intersection of the classical pathway, the alternative pathway and the mannan-binding clusterin pathway of complement activation, and is also the core of the complement system. Acute and chronic glomerulonephritis, systemic lupus erythematosus, rheumatoid arthritis and other diseases often cause the decrease of human serum complement. Low complement C3 content often leads to severe suppurative infection, pneumonia, meningitis, and sepsis, etc., which may be related to the greatly reduced ability of the body to resist infection, and the loss of C3-mediated dissolution and clearance of circulating immune complexes (CIC); and Excessive C3 content can...

AI Technical Summary

Problems solved by technology

At present, among the many methods for detecting complement C3, only the immunoturbidimetric method has a commercial kit, which is easy to use, but the linear range is narrow and the sensitivity is not high, which limits its application in the detection of low-concentration samples. application