Sidewise-flow test strip detection kit for detecting geese origin component in food and feed and application of kit

A test strip and feed technology, applied in the fields of molecular biology and immunology, can solve the problems of increasing the complexity of the detection process and losing the convenience of the test strip method, and achieve the effects of easy promotion, high specificity, and intuitive technology

Inactive Publication Date: 2014-05-07
ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
View PDF15 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In terms of primer optimization, the invention patent with the publication number CN 102146432 A - "A method for reducing the dimer of a pair of partially homogeneous primers" describes a primer design method with a short palindromic sequence at the 5' end of the primer, The primer self-cyclizes at room temperature to avoid the formation of heterodimers. The invention patent with the publication number CN 102719547 A - "Reagent for Real-time Fluorescent Quantitative PCR for Detecting HER2 Gene Expression Level" also uses a similar method for real-time quantitative PCR Amplification; Publication No. CN 101842494 A invention patent - "use chimeric primers to reduce heterodimer formation" describes a method of using chimeric primers to amplify; in the optimization of the reaction substrate, The invention patent of publication number CN 101171343A "Containing 3' modified oligonucleotides of pseudo-isocytosine nucleic acid base derivatives and...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Sidewise-flow test strip detection kit for detecting geese origin component in food and feed and application of kit
  • Sidewise-flow test strip detection kit for detecting geese origin component in food and feed and application of kit
  • Sidewise-flow test strip detection kit for detecting geese origin component in food and feed and application of kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0050] 1 Materials and methods

[0051] Goose mtDNA

[0052] 1.2 Primer design

[0053] 1.3 PCR amplification system:

[0054] 1.3 PCR amplification system:

[0055]

[0056] Reaction conditions:

[0057]

[0058] At the same time, 5 μl was taken for nucleic acid test strip detection, 5 μL of amplification product was measured, added to 95 μL of developing solution, and detected on the sample pad, and the results were observed after 5 minutes.

[0059] 1.4 PCR specificity experiment

[0060] Use the established PCR reaction system for 1: mouse; 2: cow; 3: rabbit; 4: horse; 5: sheep; 6: dog; 7: pig; 8: cat; 9: chicken; 10: duck; 11: Goose; 12: NTC blank control (water) to verify its specificity.

[0061] 2 results

[0062] 2.1 PCR reaction system and conditions

[0063] HS Taq DNA polymerase from TaKaRa Company was used, and the total reaction system was 20 μl. Detection was performed with a Bio-Rad PCR instrument, and the reaction parameters were: 94°C for 5 min...

Embodiment 2

[0067] The sensitivity of the nucleic acid test strip detection method, the PCR template is 1 / 10, 1 / 10 2 , 1 / 10 3 , 1 / 10 4 , 1 / 10 5 , 1 / 10 6 , 1 / 10 7 , 1 / 10 8 , 1 / 10 9 , 1 / 10 10 After dilution, the PCR system was established for amplification.

[0068] 1 Materials and methods

[0069] goose mtDNA

[0070] 1.2 Primer design

[0071] 1.3 PCR amplification system:

[0072]

[0073] Reaction conditions:

[0074]

[0075] Take 5μl respectively for agarose gel electrophoresis. The gel electrophoresis conditions are: 1X TBE buffer, voltage 100V, electrophoresis time 30 minutes. At the same time, 5 μl was taken for nucleic acid test strip detection, and 5 μl of sample was spotted on the sample pad, added to 95 μL of developing solution for detection, and the results were observed after 5 minutes.

[0076] 2 results

[0077] 2.1 PCR reaction system and conditions

[0078] HS Taq DNA polymerase from TaKaRa Company was used, and the total reaction system was 20 μl. D...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a nucleic acid rapid detection kit for a geese origin component in food and feed and an application method of the kit, belonging to the fields of molecular biology and immunology. According to the invention, a high-sensitivity high-specificity method of PCR in nucleic acid detection is combined with an immuno gold staining rapid detection technology in immunological detection, extracted target DNA is subjected to specific amplification through designing a specific primer and labeling the primer and the amplified product is combined with a gold labeled antibody immobilized on the test strip in a developing liquid so as to form stable and visible detection zone and quality control zone, thereby realizing the rapid and accurate detection of geese origin component in food and feed.

Description

technical field [0001] The invention belongs to the fields of molecular biology and immunology, and relates to PCR nucleic acid amplification of goose-derived components in food and feed, and a preparation and application method of a lateral flow immune colloidal gold test strip kit. Background technique [0002] In order to prevent the spread of bovine spongiform encephalopathy (BSE) through beef and beef bones added to animal feed, the European Union and the United States in 1994 and 1997 respectively enacted a ban on adding ruminants to ruminant feed Regulations for protein of animal origin, the European Union in 2000 extended this ban to prohibit feeding processed mammalian, bird and fish protein ingredients to animals intended for food. The "Administrative Measures for the Safety and Hygiene of Animal-derived Feed Products" promulgated by the Ministry of Agriculture of my country in 2004 stipulates that the use of animal-derived feed products (except milk and dairy prod...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12Q1/68
CPCC12Q1/6804C12Q2531/113C12Q2563/131C12Q2565/625
Inventor 郑文杰贺艳陈其勇程瑜张宏伟张灿奚文辉杜敬韩宇宁尹长城刘斯奇李宏虹张亚莲
Owner ANIMAL & PLANT & FOOD INSPECTION CENT OF TIANJIN ENTRY EXIT INSPECTION & QUARANTINE BUREAU
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap