11 results about "Nucleic acid test" patented technology

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The invention relates to the field of nucleic acid test cards, and discloses a nucleic acid detection test card for storing and releasing reagents, the nucleic acid detection test card comprises a reagent card body, the left side surface of the reagent card body is provided with a liquid dropping port, and the right side surface of the reagent card body is provided with an observation window. An independent cavity mixing opening and closing mechanism is arranged on the inner wall of the left side of the reagent card body. Accurate control of'isolation-on-demand mixing 'of reagents is realized through an independent chamber mixing opening and closing mechanism: three groups of independent storage chambers are arranged in the test card and are used for respectively storing a lysis solution, an amplification reagent and the like, and complete isolation is realized through a shielding cover in an initial state, so that interaction among the reagents is avoided; during detection, the rotating block is rotated to drive the one-way tooth block to be meshed with the shielding cover tooth block, so that the shielding cover notch is aligned with each storage chamber in sequence, and the reagent is released in batches; meanwhile, a one-way limiting structure formed by the wedge block and the reset spring ensures that the shielding cover only rotates in the fixed direction.

The present application relates to a chip, a preparation method therefor, and an application thereof. The chip of embodiments of the present application comprises: a substrate having a surface grafted with an amino group, the amino group being a primary amino group or a secondary amino group; and a first compound linked to the surface via the amino group, the first compound comprising the following structure, i.e., formula (1) or formula (2), wherein R1 and R2 are each independently selected from —OH or C1-C5 alkoxy, and n is 1 or 2. The chip can be used for a nucleic acid test, such as single-molecule sequencing and next-generation sequencing.

The application provides a rapid detection method of Aspergillus fumigatus ERA / CRISPR-Cas12a, and application thereof, and further provides a corresponding kit and a method for detecting Aspergillus fumigatus. The application first establishes a rapid detection system of ERA (Enzymatic Recombinase Amplification) combined with CRISPR / Cas12a, optimizes the reaction system, and improves the sensitivity of one-pot method, the fluorescence detection limit is 1 fg / μL, the detection sensitivity of the test strip can reach 10 fg / μL, and no cross reaction occurs with other bacteria and fungi. The detection of Aspergillus fumigatus can be completed within 60 min, and the detection result is directly reflected through fluorescence and nucleic acid test strips.

A nucleic acid test cartridge, provided with a plurality of chambers (10) and at least one air vent (20). The air vent (20) is in communication with the chambers (10). The air vent (20) is used for driving a fluid in a cartridge (100) to flow among the plurality of chambers (10) when a positive pressure or a negative pressure is introduced. In addition, a water-absorbing air-permeable membrane (30) is disposed between the air vent (20) and the chambers (10) in communication therewith, and the water-absorbing air-permeable membrane (30) is used for preventing moisture from entering the cartridge (100) and eliminating air bubbles. By means of the described method, in one aspect, external moisture can be prevented from entering the cartridge (100) by means of the air vent (20) to affect test results, and in another aspect, air bubbles generated in the ultrasonic mixing process can be prevented from entering the external environment by means of the air vent (20) to cause environmental pollution.

The present application relates to the technical field of biotechnology and plant disease detection, in particular, the present application relates to a visual detection kit and detection method for pantoea ananatis based on RPA-CRISPR / Cas12a.The present application constructs specific RPA-CRISPR / Cas12a detection primers for pantoea ananatis through research and screening, and combines the method with an immunolateral flow test paper, the detection result can be directly reflected by means of nucleic acid test paper, which has the advantages of strong specificity, high sensitivity, short detection time, visualization, etc., and is not limited by materials, site and instrument, and can directly detect the target pathogenic bacteria from the disease sample, providing a simple and easy-to-use detection method for planters and primary plant protection workers.

The invention relates to the technical field of hepatitis A virus detection, and provides an application of a hepatitis A virus detection technology based on an ultra-fast reverse transcription-free exponential amplification reaction (RTF-EXPAR), which comprises a primer composition, a fragment containing HAVRNA, a conjugate DNA (BinderDNA), a trigger X (TriggerX) and a template DNA (Template X '-X'), the reagent combination is divided into a part A, a part B and a part C, the total system is 33 microliters, and the detection process is as follows: the reagents are mixed according to the sequence of the part B, the part C and the part A, and the result is interpreted through a triple nucleic acid test strip (LFD) after isothermal amplification. Through the reverse transcription-free step, the total consumed time is shortened; according to the method, expensive instruments are not needed, a base layer is adapted, the HAVRNA detection limit is as low as 1.2 * 10 <-2 > nmol, early-stage trace viruses can be detected, cross reaction is avoided, and the method is adapted to multi-scene detection.

Provided are a nucleic acid extraction module and a nucleic acid test system including the same. A nucleic acid extraction module according to an embodiment of the present invention includes an extraction base in which a nucleic acid attachment member is disposed and a first flow path connected to one side of the nucleic acid attachment member and a second flow path connected to the other side of the nucleic acid attachment member are formed, an injection needle having a hollow shape, protruding from one side of the extraction base, and connected to the first flow path, and a discharge needle having a hollow shape, protruding from the other side of the extraction base, and connected to the second flow path.

A nucleic acid test cartridge and a nucleic acid test method. The cartridge is provided with a sample inlet and a sample chamber in communication with the sample inlet. A lysis chamber, an elution chamber, and a reaction chamber, which are sequentially in communication by means of a flow channel, are arranged inside the cartridge. The sample chamber is in communication with the lysis chamber by means of a flow channel. A magnetic bead chamber is further arranged inside the cartridge. The magnetic bead chamber is in communication with the lysis chamber by means of a flow channel. A waste liquid chamber is further arranged inside the cartridge. The waste liquid chamber is in communication with the lysis chamber and the elution chamber by means of flow channels. The cartridge is provided with a first air vent in communication with at least one of the sample chamber and the lysis chamber, a second air vent in communication with the elution chamber, and a reagent inlet in communication with the lysis chamber and the elution chamber.

The invention discloses a nucleic acid test strip for detecting HPV (human papillomavirus) as well as a preparation method and a use method thereof, and belongs to the technical field of biological detection. The problem that in the prior art, a technical means for rapidly, efficiently, sensitively and quantitatively detecting HPV is lacked is solved, efficient concentration and signal amplification of target viruses in a sample are achieved through the integrated paper chip technology and the high-sensitivity fluorescence detection module, detection can be rapidly completed without enrichment culture, the advantage of rapid response is achieved, and the application prospect is wide. The on-site quick response capability and the high-throughput screening efficiency of the HPV are improved; the streptavidin detection kit is high in detection sensitivity and detection flux, specific detection DNA probes d-DNA and f-DNA and a quality control DNA probe con-DNA are designed, and the specific probe adding amount, the conjugate adding amount, NC membrane surface improvement and the mixed molar ratio of streptavidin to the probes are combined; the sensitivity detection threshold value of the nucleic acid test strip prepared by the invention is reduced to 100 CFU; and the specificity of the nucleic acid test strip provided by the invention is improved.