36 results about "Nucleic acid test" patented technology

Provided are a double-cavity micro-fluidic chip having a reaction cavity and a test cavity and used for nucleic acid test, and a test method thereof. A fluid channel is provided between the reaction cavity and the test cavity, and only one exhaust channel is provided. The combination of the fluid channel and the exhaust channel can automatically provide resistance for a sample entering the test cavity from the reaction cavity, thereby achieving a controllable transfer of the sample between the reaction cavity and the test cavity. A gravity micro-fluidic chip and a centrifugal micro-fluidic chip are designed. The gravity micro-fluidic chip has a multi-turning circuitous fluid channel of which the diameter is smaller than that of a sample injection channel, such that under the action of gravity, the sample can flow into a reaction cavity from a sample adding region but cannot flow into the fluid channel, and then under pressurization by a simple pressurization device, the sample can enter a test cavity from the reaction cavity. The centrifugal micro-fluidic chip provides an exhaust channel branched from a fluid channel, and makes the exhaust direction opposite to a rotary centrifugal direction and the position of an exhaust opening higher than that of the fluid channel; therefore, by combining the principle of internal and external balance of the atmospheric pressure and the centrifugal driving performance, the centrifugal micro-fluidic chip effectively controls the flowing of a gas and a liquid in reaction cavities and test cavities, so as to effectively control the amount of the sample entering each cavity, so that multiple nucleic acid tests can be completed simply by means of one or two times of centrifugation. In addition, for a chip which only has a reaction cavity, an exhaust channel is connected to a liquid storage cavity, and a gravity-driving chip and an asymmetric pressure-driving chip are designed, thereby effectively avoiding aerosol pollution.

The present invention belongs to the technical field of immunochromatography. Provided are a streptavidin multimer, and a preparation method therefor and the use thereof. The prepared streptavidin multimer has a strong affinity for a biotin / fluorescein nucleic acid probe. A nucleic acid test strip prepared with the prepared streptavidin multimer can realize rapid and high-sensitivity detection of a sample to be detected. Results can be obtained in 5 minutes, the false positive rate can be reduced, and the cost of the nucleic acid probe is reduced, which is convenient for non-professional technicians to easily and accurately judge the results.

The invention belongs to the technical field of meat product detection, particularly relates to a kit for detecting animal meat-derived components based on RPA-CRISPR-Cas12a and a detection method, and provides a combination of a primer pair suitable for isothermal amplification and a CRISPR / Cas12a nucleic acid detection system and a gene conserved sequence capable of specifically recognizing common meat-derived components. According to the method, RPA and CRISPR technologies are combined, the RPA primer and the crRNA sequence for detection are screened and optimized, the nucleic acid detection test strip and naked eye fluorescence color development are combined, meat-derived components in a sample can be rapidly identified without special professional equipment in the field detection process, and the method is suitable for rapid field detection of a base layer.

The invention relates to a streptavidin polymer as well as a preparation method and application thereof, and belongs to the technical field of immunochromatography. The prepared streptavidin polymer has strong affinity with a biotin / fluorescein nucleic acid probe, nucleic acid test paper prepared from the prepared streptavidin polymer can realize rapid and high-sensitivity detection on a detected sample, a result can be obtained within 5 minutes, the false positive rate can be reduced, the cost of the nucleic acid probe is reduced, and the method is suitable for popularization and application. And non-professional technicians can conveniently, easily and accurately judge the result.

1. The name of this design product: Nucleic Acid Detection Card Box. 2. Purpose of this design product: used for nucleic acid testing. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The present invention discloses a method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers, which comprises the following steps: a. test plate design; b. test number design; c. sample collection; d. sample collection with plate number replacement; e. generation of original nucleic acid test reports. In the sample collection link of the nucleic acid detection test tube laboratory of the present invention, the test tube holes in 96 plates on the test plate correspond one by one to the test tube numbers, and in the system, a nucleic acid detection sample number is automatically and uniquely corresponding to the experimental plate number, effectively reducing the error rate in the sample detection link, and at the same time, it is possible to clearly distinguish between personnel nucleic acid samples and environmental samples on the same test plate.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The invention provides an isothermal amplification heating device and method based on a phase change material, and relates to the technical field of biological detection.The device comprises a supersaturated solution container used for containing a supersaturated hydrated salt solution, and the hydrated salt solution releases heat when triggering crystallization; the phase change material layer wraps the supersaturated solution container, the phase change temperature of a phase change material is matched with the target isothermal amplification temperature, and the phase change material layer is used for absorbing heat released by hydration salt solution crystallization and maintaining the constant temperature through solid-liquid phase change; the reaction container is arranged in the phase change material layer and is used for loading a nucleic acid amplification reagent, and constant-temperature heating is realized through heat conduction of a phase change material; and the triggering device is used for triggering crystallization heat release of the supersaturated hydrated salt solution. By combining isothermal amplification and the nucleic acid detection test strip, the purpose of nucleic acid visual detection without a power supply and an instrument is achieved, and the purposes of no external power supply, no thermal sensor, low cost and portability of the device are achieved.

This invention provides a nucleic acid detection device and a nucleic acid detection method. The nucleic acid detection device comprises a bearing body, a detection body, and a motion body. The bearing body comprises a substrate part and a bridge-shaped part, the bridge-shaped part is arranged on the upper surface of the substrate part, and a sample pad is arranged on the bridge-shaped part. A channel is formed between the bridge-shaped part and the substrate part, the motion body is detachably arranged in the channel, and the motion body comprises at least one liquid absorption area and non-liquid absorption area. The nucleic acid detection method is a method for nucleic acid amplification using the sample pad and nucleic acid detection using the nucleic acid detection device. This invention solves the problem that currently lacking a device for use at home and having nucleic acid amplification and detection functions.

The invention relates to the field of nucleic acid test cards, and discloses a nucleic acid detection test card for storing and releasing reagents, the nucleic acid detection test card comprises a reagent card body, the left side surface of the reagent card body is provided with a liquid dropping port, and the right side surface of the reagent card body is provided with an observation window. An independent cavity mixing opening and closing mechanism is arranged on the inner wall of the left side of the reagent card body. Accurate control of'isolation-on-demand mixing 'of reagents is realized through an independent chamber mixing opening and closing mechanism: three groups of independent storage chambers are arranged in the test card and are used for respectively storing a lysis solution, an amplification reagent and the like, and complete isolation is realized through a shielding cover in an initial state, so that interaction among the reagents is avoided; during detection, the rotating block is rotated to drive the one-way tooth block to be meshed with the shielding cover tooth block, so that the shielding cover notch is aligned with each storage chamber in sequence, and the reagent is released in batches; meanwhile, a one-way limiting structure formed by the wedge block and the reset spring ensures that the shielding cover only rotates in the fixed direction.

The invention discloses a method for detecting bluetongue virus based on RAA-CRISPR / Cas13a, and belongs to the technical field of virus detection. According to the detection method, isothermal amplification is carried out on a non-structural protein NS3 gene of BTV by using an RAA technology, and the BTV is detected by combining the specific nucleic acid cutting capability of CRISPR / Cas13a. The sequence of the upstream primer for detecting the bluetongue virus is as shown in SEQ ID NO: 1, the sequence of the downstream primer is as shown in SEQ ID NO: 2, and the sequence of the specific crRNA is as shown in SEQ ID NO: 3. By utilizing the primer and the specific crRNA, accurate detection of bluetongue virus can be realized, and the primer and the specific crRNA are simple to operate, high in sensitivity and strong in specificity, are suitable for being used in mechanisms or epidemic situations with relatively simple instrument conditions, and are crucial to detection of BTV and prevention of bluetongue.

1. The name of this design product: Nucleic Acid Detection Card Box. 2. Purpose of this design product: used for nucleic acid testing. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention discloses a nucleic acid test strip for detecting bovine leukemia virus and a preparation and use method thereof, and belongs to the technical field of biological detection. In order to solve the problem that a technical means for rapidly, efficiently and sensitively bedside rapid detection of BLV is lacked in the prior art, the invention provides a nucleic acid test strip for detecting bovine leukemia virus, the nucleic acid test strip takes BLV-miRNAs as a bedside rapid detection target, the visual detection threshold of the nucleic acid test strip is 5.0 pM, and the detection limit is 50 pM. The detection window period of the nucleic acid test strip is earlier than that of antibody detection, and the nucleic acid test strip is high in specificity, good in repeatability, low in cost and low in false positive rate and can be used for in-situ detection; the nucleic acid test strip is combined with the using method provided by the invention, so that the bovine leukemia virus can be rapidly, efficiently and sensitively detected, the nucleic acid test strip has the characteristics of simplicity in operation, high specificity, safety, no pollution and the like, a reaction result is easy to observe, and the nucleic acid test strip can be applied to bedside rapid detection of the bovine leukemia virus.

A nucleic acid testing device includes: a stage on which is placed a tissue section to which a solution has been added, in which the solution contains a labeling substance of a target nucleic acid and an amplification reagent for the target nucleic acid; a temperature adjuster that adjusts the temperature of the tissue section on the stage; a temperature controller that controls the temperature adjuster to advance nucleic acid amplification reaction in the tissue section; an intensity detector that detects label intensity in the tissue section over time; and a storage unit that stores detection information generated by the intensity detector.

The present application relates to a chip, a preparation method therefor, and an application thereof. The chip of embodiments of the present application comprises: a substrate having a surface grafted with an amino group, the amino group being a primary amino group or a secondary amino group; and a first compound linked to the surface via the amino group, the first compound comprising the following structure, i.e., formula (1) or formula (2), wherein R1 and R2 are each independently selected from —OH or C1-C5 alkoxy, and n is 1 or 2. The chip can be used for a nucleic acid test, such as single-molecule sequencing and next-generation sequencing.

This invention provides an automated nucleic acid extraction and sample preparation device, including a frame; a working platform mounted on the frame; a sample application platform capable of moving along the Y direction; sample application deep-well plate placement positions arranged sequentially along the X direction on the sample application platform; multiple deep-well plate placement positions also arranged along the X direction on the sample application platform; a gripping and transfer module, a sample application module, and a nucleic acid extraction module mounted on the frame; the gripping and transfer module and the sample application module are movable relative to the frame along the X, Y, and Z directions, and the nucleic acid extraction module is movable relative to the frame along the X and Z directions; the gripping and transfer module moves a deep-well plate containing sample liquid to the nucleic acid extraction placement position; the nucleic acid extraction module extracts nucleic acid from the sample liquid in the deep-well plate; the gripping and transfer module grips the nucleic acid-extracted deep-well plate to the sample application deep-well plate placement position; and the sample application module extracts a predetermined amount of sample liquid from the deep-well plate to the sample application plate, forming a nucleic acid test sample.

The invention discloses a one-pot nucleic acid detection system as well as a detection method and application thereof. The system comprises Cas12a protein, crRNA, an amplification primer and PS-DNA, the PS-DNA comprises a nucleic acid fragment and a photodegradable connecting group located in the nucleic acid fragment, and phosphate ester groups of 20-24 nucleotide units in the PS-DNA are sulfated; wherein the number of nucleotide units in the nucleic acid fragment is 40nt-46nt. According to the one-pot nucleic acid detection system, the activity of CRISPR / Cas12a can be regulated and controlled by introducing photosensitive PS-DNA, and RPA amplification and a CRISPR / Cas12a detection system are successfully integrated into a one-pot reaction system. Furthermore, by using the one-pot nucleic acid detection system for nucleic acid detection, not only are the problems of sensitivity, tedious operation steps, amplification interference and the like of the CRISPR technology solved, but also the detection process is simplified, and the detection sensitivity and accuracy are remarkably improved; a new thought and a new solution are provided for popularization and popularization of a CRISPR molecular diagnosis technology in various application scenes.

The invention provides establishment and application of a rapid detection method for Aspergillus fumigatus ERA / CRISPR-Cas12a, and also provides a corresponding kit and a method for detecting Aspergillus fumigatus. According to the present invention, the rapid detection system combining ERA with CRISPR / Cas12a is established for the first time, the reaction system is optimized, the sensitivity of the one-pot method is improved, the fluorescence detection limit is 1 fg / [mu] L, the detection sensitivity of the test strip can achieve 10 fg / [mu] L, and the cross reaction with other bacteria and fungi does not occur. The aspergillus fumigatus can be detected within 60 minutes, and a detection result is intuitively reflected through fluorescence and a nucleic acid test strip.

The application provides a rapid detection method of Aspergillus fumigatus ERA / CRISPR-Cas12a, and application thereof, and further provides a corresponding kit and a method for detecting Aspergillus fumigatus. The application first establishes a rapid detection system of ERA (Enzymatic Recombinase Amplification) combined with CRISPR / Cas12a, optimizes the reaction system, and improves the sensitivity of one-pot method, the fluorescence detection limit is 1 fg / μL, the detection sensitivity of the test strip can reach 10 fg / μL, and no cross reaction occurs with other bacteria and fungi. The detection of Aspergillus fumigatus can be completed within 60 min, and the detection result is directly reflected through fluorescence and nucleic acid test strips.

A nucleic acid test kit includes a lower circular fixing cover, a circular kit arranged on the lower circular fixing cover, a sealing film arranged at a top of the circular kit, and a lower lid arranged at a lower end of the sealing film. The bottom of the lower lid is pressed against the lower end of the sealing film, and a bottom of the sealing film is pressed against the top of the circular kit; and first bolts are rotatably connected to the lower circular fixing cover, and the first bolt is in threaded connection with the lower lid through first threaded hole. Air leakage detection can be performed after encapsulation is completed, which significantly improves the quality of encapsulation compared to traditional detection kits and increases encapsulation efficiency. The sealing film can block aerosol leakage from the kit, effectively preventing cross-contamination.

1. Name of the product of this design: Graphical user interface for genetic testing for a fully automatic testing workstation (1). 2. Purpose of this design product: graphical interface interaction. 3. The key design point of this product is the graphical interface. 4. The picture or photo that best illustrates the key points of the design: main view. 5. The fully automatic inspection workstation is of existing design, and the rear view, left view, right view, top view, bottom view, and stereoscopic view are omitted. 6. Purpose of the graphical user interface: When the user is using the product, the page enters the main view. In the main view, click the "Detect" button to enter the interface change state diagram 1. In the interface change state diagram 1, click the "Next" button to enter the interface change state diagram 2. In the interface change state diagram 2, click the "Next" button to enter the interface change state diagram 3. In the interface change state diagram 3, click the "Next" button to enter the interface change state diagram 4. After the nucleic acid extraction is completed, it automatically jumps to the interface change state diagram 5. After the nucleic acid test is completed, it automatically jumps to the interface change state diagram 6. In the interface change state diagram 6, click the "Review" button in the lower right corner to enter the interface change state diagram 7. In the interface change state diagram 7, click the arc button in the lower right corner to enter the interface change state diagram 8.

The present invention provides a prevention and control method and device for cross-infection of the tested population at nucleic acid testing sampling points. The technical concept of the present invention is to aspirate the exhaled gas of the tested person through a filter membrane for filtration, so that pathogens adhere to the surface or pores of the filter membrane, and then let the filter membrane circulate and turn into the disinfection chamber, where the disinfection device inactivates the pathogens, and the filter membrane is reused; the forehead of the tested person is positioned by the sensor assembly and the isolation cloth at intervals, and information such as the body temperature and head image of the tested person is collected. When the tested person leaves, the isolation cloth and the filter membrane move into the disinfection chamber for disinfection. The isolation cloth is located between the sensor assembly and the tested person to avoid contact infection; when the tested person is sampled, the air deflector and the swing sealing plate narrow the gaps between the isolation cloth and the filter membrane entering and leaving the disinfection chamber, improving the airtightness of the disinfection chamber. The present invention avoids the problems of large volume of disinfection equipment and high disinfection energy consumption caused by the complete disinfection of the exhaled gas of the tested person, and has the advantages of good disinfection effect and high sampling efficiency.

The disclosure relates to the technical field of nucleic acid test, particularly to a nucleic acid isothermal amplification colloidal gold test device. The device comprises a lower seat and a reagent reaction module slideably arranged in the lower seat and comprising a reaction module shell, a reagent collection tube, a reagent reaction box, a solution box and a test paper installation assembly, the solution box is arranged at the bottom of the reaction module shell and located under the test paper installation assembly, the reagent reaction box is arranged on the solution box and partially located in the solution box, the reagent collection tube is arranged in the reaction module shell and the lower end is slideably matched with the reagent reaction box, and the test paper installation assembly is arranged in the reaction module shell and located outside the reagent collection tube.

The invention belongs to the field of molecular detection, and discloses a specific detection target Ps54945515 of phytophthora syringae as well as a PCR (Polymerase Chain Reaction) detection method, a fluorescence detection method and a nucleic acid test strip detection method which are established based on the target. The PCR detection method provided by the invention can amplify a 770 bp specific band aiming at the target Ps54945515, the real-time fluorescence detection method provided by the invention can realize the specific detection of the target Ps54945515, and meanwhile, the invention also establishes a rapid, specific, sensitive and portable phytophthora syringae detection method based on recombinase polymerase amplification and a nucleic acid test strip by taking the Ps54945515 as the target. Sensitivity experiments show that the genomic DNA containing 1.28 pg / mu L of phytophthora syringae generates obvious detection strips, and the sensitivity of real-time fluorescent PCR is also 1.28 pg / mu L, which indicates that the sensitivity of a new detection target is high. Therefore, the rapid detection method for the phytophthora syringae established by the invention plays an important role in developing early diagnosis of diseases caused by the phytophthora syringae through specificity and sensitivity evaluation.

A nucleic acid test cartridge, provided with a plurality of chambers (10) and at least one air vent (20). The air vent (20) is in communication with the chambers (10). The air vent (20) is used for driving a fluid in a cartridge (100) to flow among the plurality of chambers (10) when a positive pressure or a negative pressure is introduced. In addition, a water-absorbing air-permeable membrane (30) is disposed between the air vent (20) and the chambers (10) in communication therewith, and the water-absorbing air-permeable membrane (30) is used for preventing moisture from entering the cartridge (100) and eliminating air bubbles. By means of the described method, in one aspect, external moisture can be prevented from entering the cartridge (100) by means of the air vent (20) to affect test results, and in another aspect, air bubbles generated in the ultrasonic mixing process can be prevented from entering the external environment by means of the air vent (20) to cause environmental pollution.

The present application relates to the technical field of biotechnology and plant disease detection, in particular, the present application relates to a visual detection kit and detection method for pantoea ananatis based on RPA-CRISPR / Cas12a.The present application constructs specific RPA-CRISPR / Cas12a detection primers for pantoea ananatis through research and screening, and combines the method with an immunolateral flow test paper, the detection result can be directly reflected by means of nucleic acid test paper, which has the advantages of strong specificity, high sensitivity, short detection time, visualization, etc., and is not limited by materials, site and instrument, and can directly detect the target pathogenic bacteria from the disease sample, providing a simple and easy-to-use detection method for planters and primary plant protection workers.

The invention relates to the technical field of hepatitis A virus detection, and provides an application of a hepatitis A virus detection technology based on an ultra-fast reverse transcription-free exponential amplification reaction (RTF-EXPAR), which comprises a primer composition, a fragment containing HAVRNA, a conjugate DNA (BinderDNA), a trigger X (TriggerX) and a template DNA (Template X '-X'), the reagent combination is divided into a part A, a part B and a part C, the total system is 33 microliters, and the detection process is as follows: the reagents are mixed according to the sequence of the part B, the part C and the part A, and the result is interpreted through a triple nucleic acid test strip (LFD) after isothermal amplification. Through the reverse transcription-free step, the total consumed time is shortened; according to the method, expensive instruments are not needed, a base layer is adapted, the HAVRNA detection limit is as low as 1.2 * 10 <-2 > nmol, early-stage trace viruses can be detected, cross reaction is avoided, and the method is adapted to multi-scene detection.

The invention relates to the technical field of biotechnology and plant disease detection, in particular to a detection kit and a detection method for visual detection of pantoea ananatis based on RPA-CRISPR / Cas12a. A specific RPA-CRISPR / Cas12a detection primer aiming at pantoea ananatis is constructed through research and screening, the method is combined with immune lateral flow test paper, a detection result can be visually reflected by means of a nucleic acid test strip, and the method has the advantages of being high in specificity and sensitivity, short in detection time, visual and the like, is not limited by materials, sites and instruments, and can be widely applied to detection of pantoea ananatis. Target pathogenic bacteria can be directly detected from disease samples, and a simple and easy detection method is provided for growers and grass-roots plant protection workers.

The invention belongs to the technical field of nucleic acid detection, and particularly relates to an integrated nucleic acid test tube device and a using method thereof.The integrated nucleic acid test tube device comprises a reagent operation tube, and a spacing tube is arranged in the reagent operation tube and divides the reagent operation tube into an inner cavity and an annular mounting cavity; a cross-shaped spacing plate is fixed in the inner cavity, the end part of the cross-shaped spacing plate is in contact with the spacing pipe and divides the inner cavity into a plurality of treatment chambers which are respectively used for treating and cleaning a sample, a nucleic acid elution chamber is arranged at the bottom of one of the treatment chambers, and a nucleic acid elution chamber is arranged at the bottom of the other treatment chamber. Wherein silicon dioxide hydroxyl magnetic beads are preloaded in one treatment chamber and are used for adsorbing nucleic acid in a sample, and a guide structure is also mounted in the reagent operation tube. The whole process of nucleic acid detection can be completed in the same reaction system, the operation steps are greatly simplified, the detection efficiency is remarkably improved, and the pollution risk is reduced.

The utility model discloses a kit for a large-volume nucleic acid extractor, which belongs to the technical field of nucleic acid extraction and comprises a kit body provided with a locking groove component convenient for marking. The locking groove assembly comprises a cover, a frame, a locking block, a label and a locking groove, the frame is fixedly connected to the left side of the front end of the kit, the cover is rotatably connected to the right end of the frame through a hinge, the label is in contact connection with the interior of the frame, the locking groove is formed in the left end of the frame, the locking block is fixedly connected to the left side of the cover, and the locking block is in locking connection with the locking groove; the kit is provided with a kit assembly which is convenient to disassemble and assemble quickly; a reagent storage assembly is mounted on the kit. According to the mode, the labels for recording the information are placed in the frame, so that the situation that the detection content cannot be distinguished due to confusion of a plurality of groups of nucleic acid test samples is avoided, and meanwhile, the detected information is recorded, so that a worker can quickly know the sample information.