47 results about "Nucleic acid test" patented technology

Provided are a double-cavity micro-fluidic chip having a reaction cavity and a test cavity and used for nucleic acid test, and a test method thereof. A fluid channel is provided between the reaction cavity and the test cavity, and only one exhaust channel is provided. The combination of the fluid channel and the exhaust channel can automatically provide resistance for a sample entering the test cavity from the reaction cavity, thereby achieving a controllable transfer of the sample between the reaction cavity and the test cavity. A gravity micro-fluidic chip and a centrifugal micro-fluidic chip are designed. The gravity micro-fluidic chip has a multi-turning circuitous fluid channel of which the diameter is smaller than that of a sample injection channel, such that under the action of gravity, the sample can flow into a reaction cavity from a sample adding region but cannot flow into the fluid channel, and then under pressurization by a simple pressurization device, the sample can enter a test cavity from the reaction cavity. The centrifugal micro-fluidic chip provides an exhaust channel branched from a fluid channel, and makes the exhaust direction opposite to a rotary centrifugal direction and the position of an exhaust opening higher than that of the fluid channel; therefore, by combining the principle of internal and external balance of the atmospheric pressure and the centrifugal driving performance, the centrifugal micro-fluidic chip effectively controls the flowing of a gas and a liquid in reaction cavities and test cavities, so as to effectively control the amount of the sample entering each cavity, so that multiple nucleic acid tests can be completed simply by means of one or two times of centrifugation. In addition, for a chip which only has a reaction cavity, an exhaust channel is connected to a liquid storage cavity, and a gravity-driving chip and an asymmetric pressure-driving chip are designed, thereby effectively avoiding aerosol pollution.

The invention belongs to the field of miRNA detection, and particularly relates to a non-diagnostic-purpose miRNA detection method based on dual cascade amplification of a Pt nanoprobe and a kit. Comprising the following steps: 1) obtaining a Pt NPs (at) Ab nanoprobe; (2) carrying out an ISAR-CRISPR / Cas12a reaction; and (3) dripping on a nucleic acid test strip for capillary action, adding a quenching substrate into the T line for catalysis after the chromatographic reaction is finished, and judging the concentration of miRNA by observing the T line. According to the invention, a Pt nanoprobe is introduced as a fluorescence quenching probe, and after TMB is dropwise added, double-amplification cascade fluorescence quenching is generated. Due to the adoption of a double-amplification cascade fluorescence quenching strategy, the fluorescence quenching efficiency can be greatly improved, so that an ultra-low background fluorescence signal is ensured. The method has a good application prospect, can be used as a general detection method for detecting a specific nucleic acid target, and is widely applied to the fields of nucleic acid detection and the like.

The invention discloses a detection kit and a detection method for visually detecting citrus brown spot bacteria based on RPA-CRISPR / Cas12a. According to the scheme, an A.alternana visual rapid detection system based on an RPA-CRISPR / Cas12a technology is provided, the system can be carried out under the constant temperature condition of 37 DEG C, the detection lower limit is 10 <-3 > ng / mu L, the reaction time is only 40 min, the detection result can be visually reflected by means of a nucleic acid test strip, and the detection result can be compared with the prior art. The method has the advantages of high specificity, high sensitivity, short detection time, visualization and the like, is not limited by materials, sites and instruments, can detect target pathogenic bacteria from complex samples of various tissues of plants, and is simple and easy to use for farmer households and grassroots plant protection workers.

The present invention belongs to the technical field of immunochromatography. Provided are a streptavidin multimer, and a preparation method therefor and the use thereof. The prepared streptavidin multimer has a strong affinity for a biotin / fluorescein nucleic acid probe. A nucleic acid test strip prepared with the prepared streptavidin multimer can realize rapid and high-sensitivity detection of a sample to be detected. Results can be obtained in 5 minutes, the false positive rate can be reduced, and the cost of the nucleic acid probe is reduced, which is convenient for non-professional technicians to easily and accurately judge the results.

A test container, in which a sample solution is fed from a first chamber to a second chamber in order by a centrifugal force, includes: a liquid reservoir portion that stores the sample solution; a valve that includes a liquid storage portion temporarily storing the sample solution to be fed from the first chamber to the second chamber and is capable of switching between a first state where the first chamber and the liquid storage portion communicate with each other, a second state where the second chamber and the liquid storage portion communicate with each other, and a third state where both of the first chamber and the second chamber do not communicate with the liquid storage portion; and a sealing part that prevents a back flow of the sample solution after the sample solution is fed from the liquid reservoir portion to the first chamber.

The invention belongs to the technical field of meat product detection, particularly relates to a kit for detecting animal meat-derived components based on RPA-CRISPR-Cas12a and a detection method, and provides a combination of a primer pair suitable for isothermal amplification and a CRISPR / Cas12a nucleic acid detection system and a gene conserved sequence capable of specifically recognizing common meat-derived components. According to the method, RPA and CRISPR technologies are combined, the RPA primer and the crRNA sequence for detection are screened and optimized, the nucleic acid detection test strip and naked eye fluorescence color development are combined, meat-derived components in a sample can be rapidly identified without special professional equipment in the field detection process, and the method is suitable for rapid field detection of a base layer.

The invention relates to a streptavidin polymer as well as a preparation method and application thereof, and belongs to the technical field of immunochromatography. The prepared streptavidin polymer has strong affinity with a biotin / fluorescein nucleic acid probe, nucleic acid test paper prepared from the prepared streptavidin polymer can realize rapid and high-sensitivity detection on a detected sample, a result can be obtained within 5 minutes, the false positive rate can be reduced, the cost of the nucleic acid probe is reduced, and the method is suitable for popularization and application. And non-professional technicians can conveniently, easily and accurately judge the result.

1. The name of this design product: Nucleic Acid Detection Card Box. 2. Purpose of this design product: used for nucleic acid testing. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The present invention discloses a method for automatically and uniquely corresponding nucleic acid detection sample numbers to experimental plate numbers, which comprises the following steps: a. test plate design; b. test number design; c. sample collection; d. sample collection with plate number replacement; e. generation of original nucleic acid test reports. In the sample collection link of the nucleic acid detection test tube laboratory of the present invention, the test tube holes in 96 plates on the test plate correspond one by one to the test tube numbers, and in the system, a nucleic acid detection sample number is automatically and uniquely corresponding to the experimental plate number, effectively reducing the error rate in the sample detection link, and at the same time, it is possible to clearly distinguish between personnel nucleic acid samples and environmental samples on the same test plate.

The application provides a nucleic acid test strip and a detection method for detecting smut genes, and belongs to the technical field of detection. The application comprises a bottom plate, a sample pad and a water absorption pad; an NC film is attached to the bottom plate, the NC film is provided with a T line and a C line, the T line is sprayed with a coupling agent of a capture probe and streptavidin; the C line is sprayed with a coupling agent of a quality control probe and streptavidin; the quality control probe is complementary to a DNA sequence of a DNA1-AgInS2 / ZnS fluorescent probe. The application utilizes the advantages of AgInS2 / ZnS quantum dots (AgInS2 / ZnS QDs), such as good biocompatibility, high fluorescence intensity and wide spectral range. The test strip based on AgInS2 / ZnS quantum dots has higher sensitivity and stronger quantitative capacity than traditional test strips. The fluorescence ratio of the T line and the C line is detected by using a fluorescence immunoassay instrument for quantitative detection, the fluorescence immunoassay instrument can quickly read out the value, and has the advantages of rapidness, simple operation, low sensitivity, good selectivity and the like.

The invention provides an isothermal amplification heating device and method based on a phase change material, and relates to the technical field of biological detection.The device comprises a supersaturated solution container used for containing a supersaturated hydrated salt solution, and the hydrated salt solution releases heat when triggering crystallization; the phase change material layer wraps the supersaturated solution container, the phase change temperature of a phase change material is matched with the target isothermal amplification temperature, and the phase change material layer is used for absorbing heat released by hydration salt solution crystallization and maintaining the constant temperature through solid-liquid phase change; the reaction container is arranged in the phase change material layer and is used for loading a nucleic acid amplification reagent, and constant-temperature heating is realized through heat conduction of a phase change material; and the triggering device is used for triggering crystallization heat release of the supersaturated hydrated salt solution. By combining isothermal amplification and the nucleic acid detection test strip, the purpose of nucleic acid visual detection without a power supply and an instrument is achieved, and the purposes of no external power supply, no thermal sensor, low cost and portability of the device are achieved.

This invention provides a nucleic acid detection device and a nucleic acid detection method. The nucleic acid detection device comprises a bearing body, a detection body, and a motion body. The bearing body comprises a substrate part and a bridge-shaped part, the bridge-shaped part is arranged on the upper surface of the substrate part, and a sample pad is arranged on the bridge-shaped part. A channel is formed between the bridge-shaped part and the substrate part, the motion body is detachably arranged in the channel, and the motion body comprises at least one liquid absorption area and non-liquid absorption area. The nucleic acid detection method is a method for nucleic acid amplification using the sample pad and nucleic acid detection using the nucleic acid detection device. This invention solves the problem that currently lacking a device for use at home and having nucleic acid amplification and detection functions.

The invention relates to the field of nucleic acid test cards, and discloses a nucleic acid detection test card for storing and releasing reagents, the nucleic acid detection test card comprises a reagent card body, the left side surface of the reagent card body is provided with a liquid dropping port, and the right side surface of the reagent card body is provided with an observation window. An independent cavity mixing opening and closing mechanism is arranged on the inner wall of the left side of the reagent card body. Accurate control of'isolation-on-demand mixing 'of reagents is realized through an independent chamber mixing opening and closing mechanism: three groups of independent storage chambers are arranged in the test card and are used for respectively storing a lysis solution, an amplification reagent and the like, and complete isolation is realized through a shielding cover in an initial state, so that interaction among the reagents is avoided; during detection, the rotating block is rotated to drive the one-way tooth block to be meshed with the shielding cover tooth block, so that the shielding cover notch is aligned with each storage chamber in sequence, and the reagent is released in batches; meanwhile, a one-way limiting structure formed by the wedge block and the reset spring ensures that the shielding cover only rotates in the fixed direction.

The present invention relates to a multifunctional nucleic acid test tube taking and placing device and a working method thereof, comprising a support frame and a test tube rack below the support frame, wherein a nucleic acid test tube in the test tube rack is taken and placed by a nucleic acid test tube taking and placing device, a nucleic acid test tube code scanning device is provided at a side end of the test tube rack, a test tube cover opening device is provided above one end of the nucleic acid test tube code scanning device away from the test tube rack, and the test tube cover opening device is fixedly connected to the support frame, and the test tube cover opening device comprises a clamping mechanism for facilitating the clamping of the nucleic acid test tube from the nucleic acid test tube code scanning device to the test tube cover opening device, a cover opening mechanism for facilitating the twisting of the nucleic acid test tube cover, and a cotton swab cutting mechanism for cutting off excess cotton swabs, thereby realizing highly integrated taking and placing, cover opening, throat swab cotton swab cutting and test tube code scanning of nucleic acid test tubes, and effectively solving the problems of insufficient space utilization, low operating efficiency and high cost of joint mechanical arms caused by the scattered taking and placing, cover opening, throat swab cotton swab cutting and nucleic acid test tube code scanning.

The invention discloses a method for detecting bluetongue virus based on RAA-CRISPR / Cas13a, and belongs to the technical field of virus detection. According to the detection method, isothermal amplification is carried out on a non-structural protein NS3 gene of BTV by using an RAA technology, and the BTV is detected by combining the specific nucleic acid cutting capability of CRISPR / Cas13a. The sequence of the upstream primer for detecting the bluetongue virus is as shown in SEQ ID NO: 1, the sequence of the downstream primer is as shown in SEQ ID NO: 2, and the sequence of the specific crRNA is as shown in SEQ ID NO: 3. By utilizing the primer and the specific crRNA, accurate detection of bluetongue virus can be realized, and the primer and the specific crRNA are simple to operate, high in sensitivity and strong in specificity, are suitable for being used in mechanisms or epidemic situations with relatively simple instrument conditions, and are crucial to detection of BTV and prevention of bluetongue.

1. The name of this design product: Nucleic Acid Detection Card Box. 2. Purpose of this design product: used for nucleic acid testing. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: Stereoscopic drawing 1.

The invention discloses a single-tube nested PCR (Polymerase Chain Reaction) specific primer probe for detecting southern corn rust disease and a detection method of the single-tube nested PCR specific primer probe. The single-tube nested PCR specific detection primer probe is used as an inner primer pair of puccinia multiflora, the nucleotide sequence of an upstream primer of the inner primer pair is as shown in SEQ ID NO.3, and the nucleotide sequence of a downstream primer of the inner primer pair is as shown in SEQ ID NO.4. The method comprises the following steps: carrying out single-tube nested PCR (Polymerase Chain Reaction) amplification by taking puccinia multiflora genome DNA or positive plasmid DNA as a template, then carrying out immunochromatography nucleic acid test strip detection on an amplification product, and carrying out result visual judgment through a test strip. The method has high sensitivity, meanwhile, the identification time of puccinia multiflora can be shortened through the detection system, and the problems that a traditional identification method is tedious in step, long in identification period and the like are solved.

The invention discloses a nucleic acid test strip for detecting bovine leukemia virus and a preparation and use method thereof, and belongs to the technical field of biological detection. In order to solve the problem that a technical means for rapidly, efficiently and sensitively bedside rapid detection of BLV is lacked in the prior art, the invention provides a nucleic acid test strip for detecting bovine leukemia virus, the nucleic acid test strip takes BLV-miRNAs as a bedside rapid detection target, the visual detection threshold of the nucleic acid test strip is 5.0 pM, and the detection limit is 50 pM. The detection window period of the nucleic acid test strip is earlier than that of antibody detection, and the nucleic acid test strip is high in specificity, good in repeatability, low in cost and low in false positive rate and can be used for in-situ detection; the nucleic acid test strip is combined with the using method provided by the invention, so that the bovine leukemia virus can be rapidly, efficiently and sensitively detected, the nucleic acid test strip has the characteristics of simplicity in operation, high specificity, safety, no pollution and the like, a reaction result is easy to observe, and the nucleic acid test strip can be applied to bedside rapid detection of the bovine leukemia virus.

A nucleic acid testing device includes: a stage on which is placed a tissue section to which a solution has been added, in which the solution contains a labeling substance of a target nucleic acid and an amplification reagent for the target nucleic acid; a temperature adjuster that adjusts the temperature of the tissue section on the stage; a temperature controller that controls the temperature adjuster to advance nucleic acid amplification reaction in the tissue section; an intensity detector that detects label intensity in the tissue section over time; and a storage unit that stores detection information generated by the intensity detector.

The present application relates to a chip, a preparation method therefor, and an application thereof. The chip of embodiments of the present application comprises: a substrate having a surface grafted with an amino group, the amino group being a primary amino group or a secondary amino group; and a first compound linked to the surface via the amino group, the first compound comprising the following structure, i.e., formula (1) or formula (2), wherein R1 and R2 are each independently selected from —OH or C1-C5 alkoxy, and n is 1 or 2. The chip can be used for a nucleic acid test, such as single-molecule sequencing and next-generation sequencing.

A nucleic acid test kit includes a lower circular fixing cover, a circular kit arranged on the lower circular fixing cover, a sealing film arranged at a top of the circular kit, and a lower lid arranged at a lower end of the sealing film. The bottom of the lower lid is pressed against the lower end of the sealing film, and a bottom of the sealing film is pressed against the top of the circular kit; and first bolts are rotatably connected to the lower circular fixing cover, and the first bolt is in threaded connection with the lower lid through first threaded hole. Air leakage detection can be performed after encapsulation is completed, which significantly improves the quality of encapsulation compared to traditional detection kits and increases encapsulation efficiency. The sealing film can block aerosol leakage from the kit, effectively preventing cross-contamination.

This invention provides an automated nucleic acid extraction and sample preparation device, including a frame; a working platform mounted on the frame; a sample application platform capable of moving along the Y direction; sample application deep-well plate placement positions arranged sequentially along the X direction on the sample application platform; multiple deep-well plate placement positions also arranged along the X direction on the sample application platform; a gripping and transfer module, a sample application module, and a nucleic acid extraction module mounted on the frame; the gripping and transfer module and the sample application module are movable relative to the frame along the X, Y, and Z directions, and the nucleic acid extraction module is movable relative to the frame along the X and Z directions; the gripping and transfer module moves a deep-well plate containing sample liquid to the nucleic acid extraction placement position; the nucleic acid extraction module extracts nucleic acid from the sample liquid in the deep-well plate; the gripping and transfer module grips the nucleic acid-extracted deep-well plate to the sample application deep-well plate placement position; and the sample application module extracts a predetermined amount of sample liquid from the deep-well plate to the sample application plate, forming a nucleic acid test sample.

The invention discloses a one-pot nucleic acid detection system as well as a detection method and application thereof. The system comprises Cas12a protein, crRNA, an amplification primer and PS-DNA, the PS-DNA comprises a nucleic acid fragment and a photodegradable connecting group located in the nucleic acid fragment, and phosphate ester groups of 20-24 nucleotide units in the PS-DNA are sulfated; wherein the number of nucleotide units in the nucleic acid fragment is 40nt-46nt. According to the one-pot nucleic acid detection system, the activity of CRISPR / Cas12a can be regulated and controlled by introducing photosensitive PS-DNA, and RPA amplification and a CRISPR / Cas12a detection system are successfully integrated into a one-pot reaction system. Furthermore, by using the one-pot nucleic acid detection system for nucleic acid detection, not only are the problems of sensitivity, tedious operation steps, amplification interference and the like of the CRISPR technology solved, but also the detection process is simplified, and the detection sensitivity and accuracy are remarkably improved; a new thought and a new solution are provided for popularization and popularization of a CRISPR molecular diagnosis technology in various application scenes.

The invention provides establishment and application of a rapid detection method for Aspergillus fumigatus ERA / CRISPR-Cas12a, and also provides a corresponding kit and a method for detecting Aspergillus fumigatus. According to the present invention, the rapid detection system combining ERA with CRISPR / Cas12a is established for the first time, the reaction system is optimized, the sensitivity of the one-pot method is improved, the fluorescence detection limit is 1 fg / [mu] L, the detection sensitivity of the test strip can achieve 10 fg / [mu] L, and the cross reaction with other bacteria and fungi does not occur. The aspergillus fumigatus can be detected within 60 minutes, and a detection result is intuitively reflected through fluorescence and a nucleic acid test strip.

The application provides a rapid detection method of Aspergillus fumigatus ERA / CRISPR-Cas12a, and application thereof, and further provides a corresponding kit and a method for detecting Aspergillus fumigatus. The application first establishes a rapid detection system of ERA (Enzymatic Recombinase Amplification) combined with CRISPR / Cas12a, optimizes the reaction system, and improves the sensitivity of one-pot method, the fluorescence detection limit is 1 fg / μL, the detection sensitivity of the test strip can reach 10 fg / μL, and no cross reaction occurs with other bacteria and fungi. The detection of Aspergillus fumigatus can be completed within 60 min, and the detection result is directly reflected through fluorescence and nucleic acid test strips.

A test container, in which a sample solution is transferred from a first chamber to a second chamber in order by a centrifugal, includes a liquid reservoir portion that stores the sample solution in front of the first chamber, and a valve that includes a liquid storage portion temporarily storing the sample solution to be fed from the first chamber to the second chamber, in which a volume of the liquid reservoir portion is larger than a volume of the first chamber, the first chamber and the liquid reservoir portion are connected to each other only by a liquid flow channel through which the sample solution is fed, the first chamber and the second chamber are connected to each other only by a liquid flow channel through which a sample solution is fed, and a vent flow channel through which gas is released from the first chamber is not provided.

A nucleic acid test kit includes a lower circular fixing cover, a circular kit arranged on the lower circular fixing cover, a sealing film arranged at a top of the circular kit, and a lower lid arranged at a lower end of the sealing film. The bottom of the lower lid is pressed against the lower end of the sealing film, and a bottom of the sealing film is pressed against the top of the circular kit; and first bolts are rotatably connected to the lower circular fixing cover, and the first bolt is in threaded connection with the lower lid through first threaded hole. Air leakage detection can be performed after encapsulation is completed, which significantly improves the quality of encapsulation compared to traditional detection kits and increases encapsulation efficiency. The sealing film can block aerosol leakage from the kit, effectively preventing cross-contamination.

1. Name of the product of this design: Graphical user interface for genetic testing for a fully automatic testing workstation (1). 2. Purpose of this design product: graphical interface interaction. 3. The key design point of this product is the graphical interface. 4. The picture or photo that best illustrates the key points of the design: main view. 5. The fully automatic inspection workstation is of existing design, and the rear view, left view, right view, top view, bottom view, and stereoscopic view are omitted. 6. Purpose of the graphical user interface: When the user is using the product, the page enters the main view. In the main view, click the "Detect" button to enter the interface change state diagram 1. In the interface change state diagram 1, click the "Next" button to enter the interface change state diagram 2. In the interface change state diagram 2, click the "Next" button to enter the interface change state diagram 3. In the interface change state diagram 3, click the "Next" button to enter the interface change state diagram 4. After the nucleic acid extraction is completed, it automatically jumps to the interface change state diagram 5. After the nucleic acid test is completed, it automatically jumps to the interface change state diagram 6. In the interface change state diagram 6, click the "Review" button in the lower right corner to enter the interface change state diagram 7. In the interface change state diagram 7, click the arc button in the lower right corner to enter the interface change state diagram 8.

The present invention provides a prevention and control method and device for cross-infection of the tested population at nucleic acid testing sampling points. The technical concept of the present invention is to aspirate the exhaled gas of the tested person through a filter membrane for filtration, so that pathogens adhere to the surface or pores of the filter membrane, and then let the filter membrane circulate and turn into the disinfection chamber, where the disinfection device inactivates the pathogens, and the filter membrane is reused; the forehead of the tested person is positioned by the sensor assembly and the isolation cloth at intervals, and information such as the body temperature and head image of the tested person is collected. When the tested person leaves, the isolation cloth and the filter membrane move into the disinfection chamber for disinfection. The isolation cloth is located between the sensor assembly and the tested person to avoid contact infection; when the tested person is sampled, the air deflector and the swing sealing plate narrow the gaps between the isolation cloth and the filter membrane entering and leaving the disinfection chamber, improving the airtightness of the disinfection chamber. The present invention avoids the problems of large volume of disinfection equipment and high disinfection energy consumption caused by the complete disinfection of the exhaled gas of the tested person, and has the advantages of good disinfection effect and high sampling efficiency.

The disclosure relates to the technical field of nucleic acid test, particularly to a nucleic acid isothermal amplification colloidal gold test device. The device comprises a lower seat and a reagent reaction module slideably arranged in the lower seat and comprising a reaction module shell, a reagent collection tube, a reagent reaction box, a solution box and a test paper installation assembly, the solution box is arranged at the bottom of the reaction module shell and located under the test paper installation assembly, the reagent reaction box is arranged on the solution box and partially located in the solution box, the reagent collection tube is arranged in the reaction module shell and the lower end is slideably matched with the reagent reaction box, and the test paper installation assembly is arranged in the reaction module shell and located outside the reagent collection tube.