17 results about "Larix gmelini" patented technology

Association comprising or consisting of a dry extract of Polypodium leucotomos and a dry extract of Larixdecidua bark for use in the prevention of damage from exposure to UVA, UVB, UVC and / or IR rays and relative oral nutraceutical or pharmaceutical compositions.

The invention belongs to the technical field of gene engineering, and particularly relates to a larch drought response gene LgLEA10, a protein and application thereof. The nucleotide sequence of the larch drought response gene LgLEA10 provided by the invention is as shown in SEQ ID NO.1, and the amino acid sequence of the protein expressed by the larch drought response gene LgLEA10 is as shown in SEQ ID NO.2. It is proved for the first time that the LgLEA10 gene of dahurian larch is a drought response regulation gene of dahurian larch. Tests prove that overexpression of the LgLEA10 gene can improve the drought resistance of larch by remarkably improving the activity of antioxidant enzyme and osmotic adjusting substances. The discovery provides important gene resources and theoretical basis for cultivating a new variety of larch with strong drought resistance.

The invention provides a larch pine caterpillar resistance gene mining method based on GWAS and multi-omics data, and belongs to the technical field of gene mining. The method comprises the following steps: S1, carrying out insect-resistant phenotype identification on larch individuals, and carrying out variation identification on the larch individuals based on a liquid-phase gene chip sequencing technology; s2, using a mixed linear model to perform correlation analysis by taking a plot position and phenotype observation time as an interaction environment, taking population density at different observation time as a covariable and taking a larch anti-deciduous pine caterpillar index as a phenotype, and identifying a candidate QTL region; and S3, carrying out transcriptome sequencing on resistance extreme individuals, carrying out variation recognition, comparing read segments to the candidate QTL region, and manually analyzing and annotating a gene structure in the QTL region by using IGV. According to the method disclosed by the invention, low-cost variation recognition, environment noise weakening whole genome association analysis and gene structure accurate annotation on complex genome species are realized.

The invention belongs to the technical field of plant biology, and particularly relates to a protein coded by a larch LarGRF1 gene and application of the protein to plant growth promotion. The invention aims to provide a new choice for plant genetic breeding by exploring growth regulatory factors (GRFs) of larch. The technical scheme of the invention is that the protein coded by the larch LarGRF1 gene has an amino acid sequence as shown in SEQ ID No. 2. Through overexpression of the MarGRF1 gene in dicotyledonous plants and monocotyledonous plants, it is found that an overexpression vector can promote proliferation of plant cells, especially proliferation of calluses and accelerate regeneration and growth.

The invention discloses a nucleotide sequence of a larch endogenous strong promoter Pro-LarGRP1A and an application of the larch endogenous strong promoter Pro-LarGRP1A. The invention relates to a method for creating and cultivating woody gymnosperm plants such as larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like through genetic transformation of a carrier expressed by a target gene driven by a Pro-LarGRP1A promoter sequence and a method for creating and cultivating the woody gymnosperm plants such as the larch with precisely improved target agronomic characters such as biomass, disease resistance, insect resistance, stress resistance and the like. The implementation effect shows that compared with the known conventional plant expression vector, the expression vector constructed by taking the nucleotide sequence of the Pro-LarGRP1A promoter region disclosed by the invention as the promoter element is used for transforming a larch receptor material through an agrobacterium-mediated genetic transformation method, a target gene in a positive transformed strain can be efficiently expressed, and the expression vector has the advantages that the expression vector is simple and convenient to construct, and the application prospect is wide. And the gene expression level is more than 8 times that of the conventional expression vector. The nucleotide sequence of the larch endogenous strong promoter Pro-LarGRP1A and the application of the larch endogenous strong promoter Pro-LarGRP1A disclosed by the invention have an important application value in efficient and low-cost cultivation of new germplasm, new materials and new varieties of woody gymnosperm plants such as high-biomass, high-volume, high-stress-resistance and high-quality larch.

The invention relates to the technical field of tissue culture, in particular to a method for cultivating larch tissue culture seedlings and combining the larch tissue culture seedlings with soil-planted seedlings to establish vascular bundle communication to promote regeneration and growth of the tissue culture seedlings. Unrooted larch tissue culture seedlings are used as in-vitro culture seedling jointing units, one-year-old larch seedlings are used as root system supply units and the like, the in-vitro culture seedling jointing units and the root system supply units are tightly attached, then upper openings and lower openings of rubber pipes are sealed, and combined plants are obtained; and putting the combined plant into a sealed box, culturing for 40 days, moving out of the sealed box, and continuously culturing for 40 days. The limitation that rooting and transplanting are difficult in the larch tissue culture regeneration system process is broken through, the regeneration system is more perfect, and the culture time is saved. A feasible scheme is provided for breeding of a precious new variety of larch, and the method is also a breakthrough in a larch breeding technology level. Meanwhile, a technical support is provided for subsequent development of genetic improvement and industrial application of larch trees.

This invention belongs to the field of genetic engineering technology, specifically relating to improving the drought resistance of larch trees. LgERF4 Genes and their applications. (The following is a separate section...) LgERF4 The nucleotide sequence of the gene is shown in SEQ ID NO.1. This invention is the first to experimentally demonstrate... LgERF4 Gene overexpression can enhance the drought resistance of larch by significantly increasing the activity of antioxidant enzymes and the content of osmotic regulators. This discovery provides important genetic resources and theoretical basis for breeding new larch varieties with strong drought resistance.

The invention provides a larch callus ultralow-temperature storage and rapid growth recovery method, and belongs to the technical field of forest tree tissue culture breeding. The method comprises the following steps: (1) pretreating and pre-culturing calluses: taking the larch calluses, and inoculating the calluses into a pretreatment culture medium for culturing; 2) pre-culture treatment with a cryoprotectant: transferring the pre-cultured calluses into a composite cryoprotectant for soaking treatment; (3) performing segmented cryopreservation: putting the callus subjected to pre-culture treatment by a cryoprotectant into a sterile cryopreservation tube, adding a composite cryoprotectant, and performing segmented cryopreservation; 4) gradient heating unfreezing: taking out a cryopreservation tube for cryopreservation, carrying out gradient heating unfreezing, and after unfreezing, transferring the callus to a BM2 washing culture medium for washing, and 5) rapid recovery culture: inoculating the washed callus to a recovery culture medium for culture.The method provided by the invention can effectively avoid damage to the callus caused by cryopreservation.

This invention relates to the field of carbon density measurement and sampling technology, specifically a rapid carbon density measurement and sampling device for Dahurian larch trunks. The device includes a drive unit, a sampling rod connected to the output end of the drive unit, a retaining seat connected between the sampling rod and the drive unit, a protective cover slidably connected to the outer side of the sampling rod, a housing fixed to one side of the drive unit, a telescopic component fixed to the inner side of the housing, an impeller connected to one side of the meshing wheel, a filter on the air inlet side of the impeller, and two gear sets coaxially connected to the outer side of the output end of the drive unit. The impeller, by utilizing the force of the drive unit driving the sampling rod to rotate and drill, generates pressure to draw in cold air from the outside. After filtering out carbon-containing gases through the filter, the air is sent into the inner side of the sampling rod to cool it. Carbon-free gases are discharged from inside the sampling rod and flow out through the gap between the sampling rod and the trunk to the inner side of the protective cover, creating positive pressure inside the protective cover.

The invention belongs to the technical field of plant biology, and particularly relates to a larch LkWox2 gene coded protein and application thereof in plant type improvement. The technical problem to be solved by the invention is to provide a new choice for larch breeding by exploring a Wox transcription factor in larch. The technical scheme of the invention is as follows: the protein coded by the larch LkWox2 gene has an amino acid sequence as shown in SEQ ID No.2. Through overexpression of the LkWox2 gene, it is found that the gene can promote differentiation and regeneration of plant leaf disc calluses and growth of buds; growth of lateral roots and adventitious roots of plants is promoted; the plant height is also reduced, and the internode is shortened. The invention provides a new breeding direction and method for obtaining plant varieties with fast growth, high survival rate and excellent plant types.

The invention relates to the technical field of tissue culture, in particular to a regeneration method of larix olgensis. According to the method, mature zygotic embryos of larix olgensis are subjected to callus induction, adventitious bud induction, elongation culture and transplanting. According to the method, the limitation that rooting and transplanting are difficult in the process of a larix olgensis tissue culture regeneration system is broken through, an efficient and rapid tissue culture regeneration system is achieved, the period is only about 6 months, a complete regenerated plant can be obtained, and callus induction, adventitious bud induction and elongation, the rooting rate and the transplanting rate are all improved. Technical support is provided for follow-up development of genetic improvement and industrial application of Larix olgensis trees, a feasible scheme reference is provided for cultivation of a precious new variety of Larix olgensis, and the method is also a breakthrough in the propagation technology level of Larix olgensis.

The invention discloses a CEEA-exo kit and a detection method for rapidly detecting dahurian larch blight. The kit comprises a fluorescent probe NYT-1, a first constant-temperature amplification primer NYF-4 and a second constant-temperature amplification primer NYR-1, wherein nucleotide sequences of the fluorescent probe NYT-1, the first constant-temperature amplification primer NYF-4 and the second constant-temperature amplification primer NYR-1 are sequentially shown as SEQ ID NO.1, SEQ ID NO.2 and SEQ ID NO.3. A fluorescent probe NYT-1 is designed on the basis of a specific fragment of a larix gmelinii genome, the fluorescent probe NYT-1, a first constant-temperature amplification primer NYF-4, a second constant-temperature amplification primer NYR-1 and an enzyme are prepared into freeze-dried powder, A Buffer and B Buffer are prepared into microspheres, and the CEEA-exo kit is obtained through assembling. Based on the kit, the invention establishes a rapid detection method for the larix gmelinii in combination with a digital isothermal amplification technology, the method can rapidly and accurately detect target pathogenic bacteria from diseased branches, and has the advantages of strong specificity, high sensitivity, good applicability and the like; therefore, the method is of great significance to early warning of the larch shoot blight and monitoring of pathogens in quarantine areas.

This invention belongs to the field of genetic engineering technology, and more specifically, relates to a drought-responsive gene in larch trees. LgLEA10 Proteins and their applications. This invention provides a larch drought response gene. LgLEA10 The nucleotide sequence of the protein is shown in SEQ ID NO.1, and the amino acid sequence of the protein it expresses is shown in SEQ ID NO.2. This invention is the first to demonstrate that the Dahurian larch... LgLEA10 The gene is a regulatory gene for the larch's response to drought. This invention demonstrates through experiments that... LgLEA10 Gene overexpression can enhance the drought resistance of larch by significantly increasing the activity of antioxidant enzymes and osmotic regulators. This discovery provides important genetic resources and theoretical basis for breeding new larch varieties with strong drought resistance.

The invention discloses an SNP (Single Nucleotide Polymorphism) molecular marker combination and a chip for larch in North China and application of the SNP molecular marker combination and the chip. The larix gmelini SNP molecular marker combination comprises 125 SNP molecular markers and 283 SNP molecular markers, and the physical positions of the 125 SNP molecular markers and the 283 SNP molecular markers are determined on the basis of sequence alignment of a larix gmelini reference genome GWHHIXQ00000000.1. The larch SNP molecular marker combination provided by the invention has the characteristics of good representativeness, strong specificity, high polymorphism and the like, and can be widely applied to detection of different varieties and materials of larch.

The present application relates to a kind of efficient regeneration method with the larch of north China as explant, the method comprises the following steps: sowing, disinfection, induction culture, elongation culture, rooting culture, seedling and transplanting.According to the technical scheme provided by the present application, the medium with specific formula is used, so that the larch of north China shows significant advantages in the process of propagation: high differentiation efficiency, large proliferation coefficient, good rooting rate.In addition, the method also has the characteristics of short plant growth cycle, strong adaptability after transplanting and the like.The seedling after cultivation presents the characteristics of robust and straight, good growth and uniformity.The technical scheme of the present application significantly reduces the seedling culture period, effectively reduces the production cost.By establishing the adventitious bud induction system with the larch of north China as starting material, and successfully obtaining regenerated plant, a new technical approach is provided for the rapid propagation of the larch of north China.Meanwhile, the present application also lays the foundation for the future establishment of efficient larch of north China transgenic system.

The invention discloses application of a Cry13Aa1-1 gene in larch callus cultivation and pine wood nematode resistance evaluation of larch callus. The invention belongs to the technical field of biology, and relates to application of a Cry13Aa1-1 gene in larch callus culture and pine wood nematode resistance evaluation. The invention provides an agrobacterium tumefaciens-mediated plant genetic transformation method. The method comprises the following steps: infecting a plant callus by using agrobacterium tumefaciens containing a target vector to obtain an infected callus; the target vector is used for expressing the Cry13Aa1 protein. The reseparation number of the pine wood nematodes of cell lines C14 and C25 of the Cry13Aa1 protein transgenic callus is obviously lower than the reseparation number of wild callus and other transgenic cell lines, and is 3667 + / -382 and 3825 + / -865, so that the propagation of the pine wood nematodes in the calluses of the two cell lines is obviously limited, and the pine wood nematode resistance is relatively outstanding.

The invention relates to the technical field of tissue culture, in particular to a method for producing S-type lignin in coniferous trees through efficient genetic transformation. The efficient genetic transformation system research of the species is developed by taking mature zygotic embryos of larix olgensis as initial explants, a brand-new genetic transformation system of larix olgensis is established, and a foundation is laid for research and application of genetic engineering breeding, gene editing, high-quality seedling breeding and the like of the species; meanwhile, the enzyme gene CALd5H for specifically synthesizing the S-type lignin is inserted into a larch genome by utilizing an efficient genetic transformation means, so that the larch genome has an S-type lignin biosynthesis pathway, and an effective strategy is created for increasing the pulping and papermaking utilization rate of the larch.