90 results about "Canker disease" patented technology

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention discloses a citrus growth promoting fertilizer and a fertilization method thereof, and relates to the technical field of chemical fertilizer production, the citrus growth promoting fertilizer comprises 40-60 parts of macroelements, 15-25 parts of medium elements, 8-12 parts of trace elements, 10-18 parts of an organic active agent, and 5-10 parts of a slow release agent. Therefore, firstly, the nano calcium carbonate is used for replacing a traditional calcium source, and a polylactic acid-fucoidan slow-release membrane technology is combined, so that the dissolved state keeping time of calcium ions in the soil is prolonged by 30-50%, the calcium absorption rate is increased to 35% or above from the average industrial 20-30%, and the fruit cracking rate of citrus is effectively reduced; the bacillus subtilis and the seaweed extract in the organic active agent have a synergistic effect, through biological antagonism and induced resistance, the canker incidence rate is reduced to 1 / 4 of that of a control group, meanwhile, the soil microenvironment is improved through humic acid, and the root system health degree is improved by 40%.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

The application discloses a pesticide composition containing zhongshengmycin and kanamycin and application thereof, and belongs to the technical field of pesticides. Effective components of the pesticide composition are zhongshengmycin and kanamycin. The mass ratio of the kanamycin to the zhongshengmycin is 1:1-9. The two effective components of the application have different action sites for killing pathogenic bacteria, and can effectively alleviate the generation of drug resistance of the pathogenic bacteria. After the zhongshengmycin and the kanamycin are compounded, synergistic effect is obvious, the control effect of the pesticide on bacterial diseases, such as citrus canker, is improved, the pesticide dosage can be reduced, and agricultural residues are reduced. Meanwhile, the prepared soluble granules do not use any organic solvent, are safe to crops, and have no residues and pollution to the environment.

The application discloses a primer probe composition which is composed of a forward primer, a reverse primer and a probe primer. The sequence of the forward primer is shown as SEQ ID No. 1, the sequence of the reverse primer is shown as SEQ ID No. 2, and the sequence of the probe is shown as SEQ ID No. 3. The application establishes a detection method for Pseudomonas syringae pv. actidii, which comprises the following steps: extracting genomic DNA of a sample to be detected, taking the genomic DNA as a template, and performing RAA detection by using the primer composition; and determining whether the sample to be detected contains Pseudomonas syringae pv. actidii according to a strip of a LFD test paper. The detection method provided by the application has a minimum detection sensitivity of 1 pg / µL for Pseudomonas syringae pv. actidii DNA, and has a minimum detection limit of 1×10 3 cfu / mL for a bacterial suspension, and can provide a new technical means for early diagnosis and disease prevention and control of bacterial canker disease of kiwifruit.

A new cherry tree particularly distinguished dark red-purple fruit with a kidney to flat-round shape, a precocious fruiting habit, self-fertility, good resistance to rain crack and moderate resistance to bacterial canker, is disclosed.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The invention discloses application of phenylpyruvic acid in prevention and control of citrus canker, and belongs to the field of treatment of agricultural canker. The invention discloses a new application of phenylpyruvic acid in treating and / or preventing citrus canker, and the effective dosage of phenylpyruvic acid is 0.01-1.5 M. The phenylpyruvic acid is externally applied, so that the scab area of the citrus canker can be reduced to a great extent, and the attack degree of the canker is reduced. In addition, the content of phenylpyruvic acid in citrus can be increased through multiple technologies, and the phenylpyruvic acid can be used for canker-resistant molecular breeding.

The application provides a method for improving the resistance of citrus to canker disease based on gene interference, which comprises the following steps: introducing a long-chain fatty alcohol oxidase gene in a citrus plant into an interference vector as an interference sequence, and integrating the interference sequence into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. CsFAO3 The part of the coding sequence of the gene, i.e., the sequence as shown in SEQ ID NO: 3, is used as an interference sequence, introduced into an interference vector, and integrated into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. The method can directly inhibit most of the pathogenic bacteria causing the canker disease of the citrus, the gene to be interfered with can greatly improve the resistance of the citrus to the canker disease while reducing the adverse effects on the known quality and performance of the citrus to the greatest extent, a new way for the breeding of the citrus resistant to the canker disease is opened up, and the development of the breeding of the citrus resistant to the canker disease is promoted.

The application provides a Yunnan golden camellia root endophytic fungus and application thereof, and relates to the technical field of crop disease prevention and treatment. The Yunnan golden camellia root endophytic fungus is Lecanicillium attenuatum JHC098, which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 2025749. The liquid fermentation broth extract of the fungus can be applied to crop disease prevention and treatment. The application overcomes the defects of the prior art. The liquid fermentation broth extract of the Yunnan golden camellia root endophytic fungus Lecanicillium attenuatum JHC098 can effectively inhibit the growth of citrus canker and tobacco bacterial wilt pathogenic bacteria, so that the purpose of biological prevention is achieved.

This novel nonpathogenic bacterial strain exhibiting a controlling effect on crown gall disease in plants and tomato canker disease is the Allorhizobium vitis A3 strain (accession number: NITE BP-041154).

The invention provides a carpet grass xanthomonas phage, a compound phage preparation and application of the carpet grass xanthomonas phage and the compound phage preparation. The carpet grass xanthomonas phage is carpet grass xanthomonas phage XAC17P1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241062. The compound bacteriophage preparation provided by the invention has higher sterilization efficiency and better application stability, belongs to a green and efficient plant disease biological prevention and control means, and can effectively prevent and treat citrus canker.

A new cherry tree particularly distinguished dark red fruit with a flat-round shape, a very precocious fruiting habit, self-fertility, very tolerant to rain crack and moderate resistance to bacterial canker, is disclosed.

This invention relates to a Bacillus tequilensis microbial agent, its preparation method, and its application, belonging to the field of agricultural microbiology. A Bacillus tequilensis YP131 microbial agent, with accession number CGMCC No. 26340, was deposited at the China General Microbiological Culture Collection Center on December 27, 2022. The Bacillus tequilensis YP131 (CGMCC No. 26340) microbial agent of this invention can significantly reduce the occurrence of summer canker in kiwifruit. Spraying with the Bacillus tequilensis YP131 microbial agent showed a control efficacy of 75.01% against summer canker in kiwifruit.

A new cherry tree particularly distinguished self-fertility, resistance to rain crack and resistance to bacterial canker, is disclosed.

The invention discloses a bacillus amyloliquefaciens strain and application thereof. The strain is bacillus amyloliquefaciens A16, and the preservation number of the strain is CGMCC (China General Microbiological Culture Collection Center) NO. 33988. The strain is separated from diseased rhizosphere soil of wine grape cabernet sauvignon in Ningxia Yongning County, and has a remarkable antagonistic effect on grape canker pathogenic bacteria (Lasiodiplodia theobroma). Researches show that the strain can secrete siderophore and protease, and the inhibition rates of thalli, volatile organic compounds (VOCs) and fermentation filtrate on the growth of pathogenic hypha are 75.43%, 66.25% and 45.33% respectively. An in-vitro branch test determines that the optimal application concentration is 1 * 10 < 7 > CFU / mL, and the prevention and treatment effects are better than those of a chemical agent carbendazim. Pot experiment results show that the prevention and control effect of the strain on grape canker reaches 81.48%, and the strain shows good biocontrol application potential.

The invention discloses application of any compound of 8-substituted berberine derivatives in prevention and treatment of agricultural diseases. A biological activity test shows that the derivative has a significant effect on four plant pathogenic bacteria such as Xanthomonas oryzae pv. Oryzae, Xanthomonas citri, pseudomonas solanacearum and Ralstonia solanacearum, and four plant pathogenic fungi such as rhizoctonia solanacearum, sclerotinia sclerotiorum, botrytis cinerea and fusarium graminearum. The compound shows certain inhibitory activity on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes, and has a good inhibitory effect on pathogenic bacteria of rice bacterial leaf blight, pathogenic bacteria of citrus canker, rhizoctonia solani, sclerotinia sclerotiorum, botrytis cinerea and pine wood nematodes. The compound is low in preparation difficulty, raw materials are cheap and easy to obtain, and the compound is expected to be developed into a novel plant disease resistant medicine.

The invention provides rhodopseudomonas palustris PSB-S, a biocontrol microbial agent thereof and application of the rhodopseudomonas palustris PSB-S in prevention and treatment of kiwifruit canker, and belongs to the technical field of biopesticides. The strain is preserved in the China General Microbiological Culture Collection Center (CGMCC) on November 17, 2025, and the preservation number is CGMCC No.36662. The invention further discloses a preparation method of the strain. According to the method, the biocontrol inoculant is obtained by culturing the rhodopseudomonas palustris PSB-S, and the kiwifruit canker is prevented and controlled by spraying the whole plant. The application of the biocontrol inoculant can induce the increase of the activity of defensive enzymes of kiwi fruit plants, and the defensive enzymes comprise at least one of peroxidase (POD), superoxide dismutase (SOD), phenylalanine ammonialyase (PAL) and catalase (CAT). By applying the biocontrol inoculant, the expression of immune response genes NPR1 and FLS2 of kiwi fruit plants can be up-regulated, and the biocontrol inoculant is environment-friendly and has no acute toxic harm. The invention is expected to provide a direction for prevention and control of kiwifruit canker and sustainable development of agriculture in China.

This divisional application discloses the use of a 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative in the control or treatment of agricultural pathogenic bacteria. The structural formula of the 2,8-bis(trifluoromethyl)-4-hydroxyquinoline derivative is as follows: Bioactivity tests show that this derivative exhibits excellent inhibitory activity against two plant bacteria: rice bacterial blight pathogen and citrus canker pathogen. Compound Q-29 shows an MIC90 value of 1.56 μg / mL against both rice bacterial blight pathogen and citrus canker pathogen, significantly outperforming the control drugs thiabendazole and thiabendazole copper. The compounds involved in this invention are easy to prepare, use inexpensive and readily available raw materials, and are expected to be developed into a novel agricultural antibacterial agent.

This invention belongs to the field of biotechnology. To improve the effectiveness of biological control of plant diseases, this invention discloses *Bacillus velezensis* ZF516 and its application in controlling various soil-borne diseases. The *Bacillus velezensis* strain number is ZF516, and its registration number at the China General Microbiological Culture Collection Center is CGMCC No. 30136. This *Bacillus velezensis* strain can prevent and / or treat tomato bacterial canker, tomato bacterial wilt, or cowpea root rot, or can be used to prepare products for the prevention and / or treatment of these diseases. This strain also exhibits good inhibitory effects against *Fusarium oxysporum*, *Diplostomum spp.*, *Rhizoctonia solani*, *Fusarium solanum*, *Corynebacterium multiflorum*, *Botrytis cinerea*, *Agrobacterium tumefaciens*, *Pectinobacter brasiliensis*, *Ralstonia solanacearum*, *Streptomyces spp.*, etc.

A new cherry tree particularly distinguished self-fertility, resistance to rain crack and resistance to bacterial canker, is disclosed.

The invention relates to the technical field of agriculture, and particularly discloses a method for preventing citrus canker and phylloxera and application. According to the method, different prevention agents are sprayed in the spring shoot period, the flowering period, the late summer shoot period and the autumn shoot period of plants respectively, so that the purpose of efficiently preventing and treating citrus canker and phylloxera is achieved. By means of the method for preventing the citrus canker and the phylloxera, the citrus canker and the phylloxera can be effectively prevented, plants suffering from the citrus canker and the phylloxera can be treated, and the method is easy and convenient to operate and free of toxic and side effects. The invention effectively solves the problems that a single chemical agent is easy to generate drug resistance in the prior art, the research and development cost of a biological agent is high, and the prevention and treatment effects of the chemical agent and the biological agent are limited.

This invention relates to the field of pesticides, specifically to a fungicide composition for foliar spraying, its preparation method, and application scheme. The active ingredients of the fungicide composition include quinoline copper and phosphite A. Phosphite A is selected from at least one of phosphorous acid, alkali metal salts or alkaline earth metal salts of phosphorous acid, alkylphosphonic acids containing C1-C6 groups of phosphorous acid or alkali metal salts of alkylphosphonic acids, alkaline earth metal salts, and aluminum salts. In the fungicide composition, the weight ratio of quinoline copper to phosphite A is 99:1 to 1:99. The fungicide composition of this invention has high activity against bacterial and fungal diseases occurring in various crops, such as canker and downy mildew, and also exhibits significant synergistic effects. It not only improves the antibacterial or bacteriostatic effect but also broadens the control spectrum. Simultaneously, it can reduce the number of applications and alleviate environmental pressure, thereby achieving the effect of prevention-oriented and integrated pest management.

The invention relates to application of a metabolite of bacillus methylotrophicus GBAIL-9 in prevention and treatment of kiwifruit canker, the classification name of the bacillus methylotrophicus GBAIL-9 is bacillus methylotrophicus, and the preservation number of the bacillus methylotrophicus GBAIL-9 is CGMCC (China General Microbiological Culture Collection Center) No: 13337.

This invention discloses the application of the natural product santalol as an agricultural fungicide in plant disease control, belonging to the field of plant disease prevention and control. It shows good inhibitory effects against bacterial diseases such as rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwifruit canker, tobacco bacterial wilt, cucumber bacterial angular leaf spot, and potato bacterial wilt. It also shows good inhibitory effects against viral diseases such as tobacco mosaic virus; and against fungal diseases such as rice rhizome blight, wheat scab, pepper wilt, *Botrytis cinerea*, or *Colletotrichum sorghum*. It can be used in the preparation of plant fungicides. Santalol is a natural plant extract and is environmentally friendly. This invention provides a new use for the natural product santalol, enabling its application in the agricultural field for plant disease control and providing a new avenue for the research and development of new pesticides.

The invention discloses a cubilose fruit grafting seedling raising method, and belongs to the technical field of fruit tree cultivation. The method comprises six aspects of stock planting field establishment, stock seedling raising, stock water and fertilizer management, grafting, grafted seedling water and fertilizer management and grafted seedling field management. A special cutter tool for cubilose fruit grafting is adopted, an L-shaped section can be formed, the section is smooth and good in integrating degree, and the grafting survival rate and the grafting efficiency can be improved. According to the invention, the seedling raising grids are used for stock seedling raising, so that the seedling raising cost can be saved, and the space utilization rate can be improved. According to the method, the thorn-free huanglong and the argentodoxa cuneata are adopted as the stocks, the root systems are developed, the morbidity of root-knot nematodes can be reduced, the grafting survival rate and the growth vigor are improved, and meanwhile the canker resistance of plants is improved; the quality of the rootstock is guaranteed, the grafting growth vigor is improved, bud germination is promoted, and fruit bearing is carried out in advance by optimizing the length, the period and the number of bud points of the rootstock.

The invention relates to the field of pesticides, and provides an agricultural composition as well as a preparation method and application thereof. The invention provides application of an agricultural composition in preparation of a pesticide bactericide and / or a plant growth regulator. The active ingredients of the agricultural composition comprise an aloe extract and rhamnolipid. According to the present invention, the aloe extract and the rhamnolipid are adopted as the active components of the agricultural composition, and through the specific ratio, the two active components can achieve the synergistic interaction, and particularly have the excellent prevention and control effect on bacterial infected plant diseases, such as the significant effect on the prevention and control of rice bacterial streak, citrus canker and cucumber bacterial angular leaf spot, meanwhile, the growth of new shoots and new leaves of the plants can be promoted, and the method has excellent commercial value.

The invention discloses pantoea for efficiently preventing and treating citrus canker and application of the pantoea. The pantoea is named as Pantoea cypripedii Q210, the preservation number is CGMCC NO.36920, the preservation date is December 8, 2025, and the preservation site is the China General Microbiological Culture Collection Center. The bacterial strain is separated and screened from soil of a Gannan navel orange orchard, and it is found that the bacterial strain has the capacity of inhibiting growth of citrus canker pathogen Xcc, has an obvious prevention and treatment effect on citrus canker and can provide a new biological prevention and treatment means for comprehensive prevention and treatment of citrus canker.