143 results about "Canker disease" patented technology

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention discloses a Burkholderia cepacia MZ-8 which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on April 8, 2025, and the preservation number of the Burkholderia cepacia MZ-8 is GDMCC No: 66106. The research finds that the burkholderia cepacia MZ-8 can efficiently inhibit the growth of pathogenic bacteria of citrus canker, the inhibition rate of the burkholderia cepacia MZ-8 to the pathogenic bacteria of citrus canker reaches 98.27% in a pot culture test, and meanwhile, the burkholderia cepacia MZ-8 can dissolve organic phosphorus, generate iron carriers and fix nitrogen and promote plant growth. Therefore, the burkholderia cepacia MZ-8 disclosed by the invention has good application potential in the aspects of preventing and treating citrus canker, promoting absorption and growth of phosphorus, iron and nitrogen of plants and the like. The discovery of the bacterial strain enriches beneficial microbial resources for biocontrol and growth promotion, and provides a bacterial strain which is stable in antibacterial effect, efficient and environment-friendly for green biological control.

The invention relates to the technical field of agricultural biological genes, and discloses a method for improving citrus canker resistance based on a CsKCS2 gene, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsKCS2 gene in citrus plants. The method specifically comprises the following steps: (1) cloning a citrus CsKCS2 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsKCS2 gene expression level. By cloning the coding sequence of the citrus CsKCS2 gene, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsKCS2 overexpression transgenic plant can be reduced to 73.3% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the application prospect is broad. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention belongs to the technical field of genetic engineering, and relates to application of a transcription factor AcWRKY20 in resisting kiwifruit bacterial canker. Through gene cloning, phylogenetic analysis, subcellular localization, transgenic function verification and other technologies, it is proved for the first time that the transcription factor AcWRKY20 has the molecular characteristic of remarkably enhancing the disease resistance of kiwi fruits. According to the invention, the AcWRKY20 gene is respectively silenced and overexpressed and then is inoculated with Psa, and phenotypic observation shows that the gene is a disease-resistant gene and is suitable for canker-resistant breeding of kiwi fruit.

The invention discloses a citrus growth promoting fertilizer and a fertilization method thereof, and relates to the technical field of chemical fertilizer production, the citrus growth promoting fertilizer comprises 40-60 parts of macroelements, 15-25 parts of medium elements, 8-12 parts of trace elements, 10-18 parts of an organic active agent, and 5-10 parts of a slow release agent. Therefore, firstly, the nano calcium carbonate is used for replacing a traditional calcium source, and a polylactic acid-fucoidan slow-release membrane technology is combined, so that the dissolved state keeping time of calcium ions in the soil is prolonged by 30-50%, the calcium absorption rate is increased to 35% or above from the average industrial 20-30%, and the fruit cracking rate of citrus is effectively reduced; the bacillus subtilis and the seaweed extract in the organic active agent have a synergistic effect, through biological antagonism and induced resistance, the canker incidence rate is reduced to 1 / 4 of that of a control group, meanwhile, the soil microenvironment is improved through humic acid, and the root system health degree is improved by 40%.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The application discloses a kiwi bacterial canker disease susceptible gene AcADC1 and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the AcADC1 gene is shown in SEQ ID NO:1, and the arginine decarboxylase (ADC) synthesized by the gene is encoded, and the amino acid sequence is shown in SEQ ID NO:2, and the arginine (Arg) is responsible for catalyzing the formation of putrescine. The resistance of the kiwi plant with the silenced AcADC1 gene to the bacterial canker disease is improved, the resistance of the kiwi plant with the overexpressed AcADC1 gene to the bacterial canker disease is reduced, the AcADC1 gene plays a negative regulation role in the immune response of the kiwi to the bacterial canker disease, the gene editing technology can be used for editing the AcADC1 gene, and a theoretical basis and gene reserve are provided for kiwi disease-resistant breeding.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

The application discloses a pesticide composition containing zhongshengmycin and kanamycin and application thereof, and belongs to the technical field of pesticides. Effective components of the pesticide composition are zhongshengmycin and kanamycin. The mass ratio of the kanamycin to the zhongshengmycin is 1:1-9. The two effective components of the application have different action sites for killing pathogenic bacteria, and can effectively alleviate the generation of drug resistance of the pathogenic bacteria. After the zhongshengmycin and the kanamycin are compounded, synergistic effect is obvious, the control effect of the pesticide on bacterial diseases, such as citrus canker, is improved, the pesticide dosage can be reduced, and agricultural residues are reduced. Meanwhile, the prepared soluble granules do not use any organic solvent, are safe to crops, and have no residues and pollution to the environment.

This invention relates to the field of agricultural pest control, and particularly to a compound nanogel sustained-release agent, its preparation method, and its application. This invention provides a compound nanogel sustained-release agent comprising bromonitol, a synergist, a carrier material, and a cross-linking agent, wherein the synergist includes one or more of kasugamycin, amino oligosaccharides, thiazolium zinc, and copper hydroxide. This invention prepares a nanogel sustained-release agent by compounding bromonitol and the synergist, which has significant preventive effects against plant bacterial diseases such as bacterial wilt, bacterial angular leaf spot, canker, and soft rot. Furthermore, the compound nanogel sustained-release agent of this invention has small particle size, stable physicochemical properties under cold and heat, good dispersibility in water, no particle agglomeration, no particle size increase, a decomposition rate of less than 5%, a long-lasting effect, good safety, is not prone to inducing drug resistance, requires a small dosage, and simultaneously increases yield and efficiency.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

The invention relates to the technical field of microbial compositions, in particular to a microbial composition for preventing and treating citrus canker and application of the microbial composition. According to the microbial composition provided by the invention, the pseudomonas Qingdao microbial agent, sodium lignin sulfonate, trehalose, glutathione, ascorbic acid, lecithin and trace elements (Zn, B and Mn) are taken as main raw materials, and the effects of efficiently inhibiting bacteria, remarkably inducing and improving the activity of key defensive enzymes of leaves, promoting synthesis and accumulation of disease-resistant substances and improving the disease resistance of the leaves are achieved through the matching effect of all the components. The diversity and richness of citrus phyllospheric bacterial communities are improved, and the purpose of constructing a stable community dominated by beneficial bacteria is achieved. In other words, the basic stress resistance of the plants can be enhanced, and meanwhile, the growth and metabolism activity of beneficial bacteria is also promoted, so that effective, eco-friendly and sustainable prevention and control of the citrus canker are achieved.

The invention belongs to the technical field of prevention and control of plant bacterial diseases, and particularly relates to an amide derivative containing benzoxazole sulfone and a preparation method and application thereof.The amide compound containing benzoxazole sulfone has good antibacterial activity on plant pathogenic bacteria of rice bacterial leaf blight, rice bacterial streak and citrus canker, and can be used for preventing and controlling bacterial diseases of plants such as rice bacterial leaf blight, rice bacterial streak and citrus canker. The method has the advantages of simple preparation process, excellent reaction yield and low raw material cost; the structural formula of the derivative is shown as I,

The application discloses a primer probe composition which is composed of a forward primer, a reverse primer and a probe primer. The sequence of the forward primer is shown as SEQ ID No. 1, the sequence of the reverse primer is shown as SEQ ID No. 2, and the sequence of the probe is shown as SEQ ID No. 3. The application establishes a detection method for Pseudomonas syringae pv. actidii, which comprises the following steps: extracting genomic DNA of a sample to be detected, taking the genomic DNA as a template, and performing RAA detection by using the primer composition; and determining whether the sample to be detected contains Pseudomonas syringae pv. actidii according to a strip of a LFD test paper. The detection method provided by the application has a minimum detection sensitivity of 1 pg / µL for Pseudomonas syringae pv. actidii DNA, and has a minimum detection limit of 1×10 3 cfu / mL for a bacterial suspension, and can provide a new technical means for early diagnosis and disease prevention and control of bacterial canker disease of kiwifruit.

A new cherry tree particularly distinguished dark red-purple fruit with a kidney to flat-round shape, a precocious fruiting habit, self-fertility, good resistance to rain crack and moderate resistance to bacterial canker, is disclosed.

The invention discloses a method for preventing and treating kiwifruit canker by using a traditional Chinese medicine fertilizer. According to the method, the occurrence and development of the kiwifruit canker are effectively controlled through disease monitoring and evaluation, agricultural prevention and treatment measures and application of a comprehensive prevention and treatment strategy of matching traditional Chinese medicine fertilizers and other medicaments based on a traditional Chinese medicine agricultural technology. Wherein the traditional Chinese medicine fertilizer takes a Chinese herbal medicine plant-derived traditional Chinese medicine fertilizer with sterilization and bacteriostasis effects as a core, and is precisely applied in different growth stages in combination with a plurality of prevention and treatment medicaments. The application concentration, frequency and opportunity of the traditional Chinese medicine fertilizer and other medicaments as well as disease monitoring and local treatment measures are specified in detail. By implementing the method, the disease resistance and fruit quality of the kiwi fruits can be remarkably improved, meanwhile, the use amount of chemical pesticides is reduced, and the ecological environment is protected. The method has the advantages of being environmentally friendly, efficient, sustainable and the like, and a new technical approach is provided for green prevention and treatment of the kiwifruit canker.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of tryptophanone in prevention and treatment of plant bacteria and part of plant fungi. The present application discloses that natural product tryptophanone can effectively prevent and treat plant bacterial and part of plant fungal diseases. The application of tryptophanone as an agricultural bactericide in plant bacterial diseases has excellent inhibitory effect on rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwi canker, tobacco bacterial wilt, cucumber bacterial angular spot, potato bacterial wilt, soybean bacterial spot, tomato canker and the like. The tryptophanone also shows good inhibitory activity on plant pathogenic fungi such as grapevine Eutypa lata, tobacco alternaria alternata, eggplant verticillium wilt, pepper fusarium wilt, colletotrichum gloeosporioides, tea tree colletotrichum gloeosporioides and sorghum zylosporium.

This invention discloses the antimicrobial peptide PJX13 and its application in the control of citrus canker. The sequence of the antimicrobial peptide PJX13 is AcKKFWKLRRWVKWIGNH2. It can effectively inhibit and kill *Xanthomonas citrus* by disrupting the cell membrane integrity of pathogens, leading to leakage of intracellular substances. It effectively inhibits the expansion of lesions on both detached citrus leaves and living plants, and can be formulated into an antimicrobial product against *Xanthomonas citrus* for the control of citrus canker. Furthermore, the antimicrobial peptide PJX13 can also inhibit *Escherichia coli*, *Staphylococcus aureus*, and *Bacillus oryzae*, and can be used to prepare broad-spectrum antimicrobial products. This invention is beneficial for the control of citrus canker, and has advantages such as being environmentally friendly and unlikely to induce drug resistance.

The invention discloses application of phenylpyruvic acid in prevention and control of citrus canker, and belongs to the field of treatment of agricultural canker. The invention discloses a new application of phenylpyruvic acid in treating and / or preventing citrus canker, and the effective dosage of phenylpyruvic acid is 0.01-1.5 M. The phenylpyruvic acid is externally applied, so that the scab area of the citrus canker can be reduced to a great extent, and the attack degree of the canker is reduced. In addition, the content of phenylpyruvic acid in citrus can be increased through multiple technologies, and the phenylpyruvic acid can be used for canker-resistant molecular breeding.

The application provides a method for improving the resistance of citrus to canker disease based on gene interference, which comprises the following steps: introducing a long-chain fatty alcohol oxidase gene in a citrus plant into an interference vector as an interference sequence, and integrating the interference sequence into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. CsFAO3 The part of the coding sequence of the gene, i.e., the sequence as shown in SEQ ID NO: 3, is used as an interference sequence, introduced into an interference vector, and integrated into the citrus genome by means of agrobacterium-mediated genetic transformation, so as to effectively improve the resistance of the citrus to canker disease. The method can directly inhibit most of the pathogenic bacteria causing the canker disease of the citrus, the gene to be interfered with can greatly improve the resistance of the citrus to the canker disease while reducing the adverse effects on the known quality and performance of the citrus to the greatest extent, a new way for the breeding of the citrus resistant to the canker disease is opened up, and the development of the breeding of the citrus resistant to the canker disease is promoted.

The application provides a Yunnan golden camellia root endophytic fungus and application thereof, and relates to the technical field of crop disease prevention and treatment. The Yunnan golden camellia root endophytic fungus is Lecanicillium attenuatum JHC098, which is preserved in the China Center for Type Culture Collection and has a preservation number of CCTCC NO: M 2025749. The liquid fermentation broth extract of the fungus can be applied to crop disease prevention and treatment. The application overcomes the defects of the prior art. The liquid fermentation broth extract of the Yunnan golden camellia root endophytic fungus Lecanicillium attenuatum JHC098 can effectively inhibit the growth of citrus canker and tobacco bacterial wilt pathogenic bacteria, so that the purpose of biological prevention is achieved.

This novel nonpathogenic bacterial strain exhibiting a controlling effect on crown gall disease in plants and tomato canker disease is the Allorhizobium vitis A3 strain (accession number: NITE BP-041154).

This invention discloses a method and application for enhancing citrus canker resistance using the CsMBL1 gene, relating to the field of agricultural biotechnology. The method for enhancing citrus canker resistance using the CsMBL1 gene includes the following steps: (1) cloning the citrus CsMBL1 coding sequence, as shown in SEQ ID No. 1; (2) constructing a CsMBL1 overexpression vector; (3) transforming citrus with the CsMBL1 overexpression vector, and after cultivation, obtaining transgenic plants with enhanced canker resistance. This invention effectively enhances citrus resistance to canker by integrating a citrus globular mannose-specific lectin binding gene into citrus through an expression vector, which has significant application value for citrus canker resistance breeding.

The invention provides a carpet grass xanthomonas phage, a compound phage preparation and application of the carpet grass xanthomonas phage and the compound phage preparation. The carpet grass xanthomonas phage is carpet grass xanthomonas phage XAC17P1, and the preservation number is CCTCC (China Center for Type Culture Collection) NO: M 20241062. The compound bacteriophage preparation provided by the invention has higher sterilization efficiency and better application stability, belongs to a green and efficient plant disease biological prevention and control means, and can effectively prevent and treat citrus canker.

A new cherry tree particularly distinguished dark red fruit with a flat-round shape, a very precocious fruiting habit, self-fertility, very tolerant to rain crack and moderate resistance to bacterial canker, is disclosed.

The present invention discloses a traditional Chinese medicine extract for preventing and treating kiwifruit canker, as well as its preparation method and application. The extract comprises the following components: an extract of Radix Isatidis and / or an extract of Rhizoma Cibotii. The extract has the potential to prevent and treat kiwifruit canker pathogens and has the potential to be developed into a new botanical fungicide. Furthermore, the extract is made from traditional Chinese medicinal materials and has less impact on humans, animals, and the environment than chemical pesticides.

The application discloses application of a carbazole derivative in prevention and treatment of plant diseases and relates to the technical field of medicinal chemistry. The structure of the carbazole derivative is as follows: The carbazole derivative has excellent inhibiting effect on plant pathogenic bacteria such as Xanthomonas oryzae ACCC 11602, Xanthomonas axonopodis pv.Citri, Pseudomonas soli maceraum and Erwinia aroideae, and has the advantages of simple structure, cheap raw materials and easy synthesis, and has the value of further research and development.