195 results about "Canker disease" patented technology

The invention provides Yunnan golden camellia root endophytic fungi and application thereof, and relates to the technical field of crop disease control. The Yunnan camellia nitidissima root endophytic fungus is Lecanicillium vase JHC098 and is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2025749, and a liquid fermentation liquor crude extract of the Lecanicillium vase can be applied to prevention and treatment of crop diseases. The invention overcomes the defects in the prior art, and the crude extract of the liquid fermentation liquor of the endophytic fungus Lecanicillium viculare JHC098 at the root of Yunnan golden camellia can effectively inhibit the growth of pathogenic bacteria such as citrus canker and tobacco bacterial wilt, thereby achieving the purpose of certain biological control.

The invention discloses a method for improving citrus canker resistance through a CsAHL15 gene and application of the CsAHL15 gene, and relates to the field of agricultural biological genes. A method for improving citrus canker resistance through CsAHL15 comprises the following steps that 1, a citrus CsAHL15 coding sequence is cloned, and the CsAHL15 coding sequence is shown as SEQ ID No.1; (2) constructing a CsAHL15 overexpression vector; and (3) CsAHL15 overexpression vector transformation citrus, and identification is carried out to obtain a transgenic plant with improved canker resistance. According to the invention, the citrus CsAHL15 coding sequence is cloned, the overexpression vector is constructed, and then the citrus is transformed, so that the canker attack degree of the obtained transgenic plant can be maximally reduced to 40.09% of that of the existing citrus, the canker attack degree can be obviously reduced, and the scab area can be reduced. In conclusion, the method is a potential biological engineering technology for improving the citrus canker resistance, and has a great value for citrus canker resistance molecular breeding.

The invention belongs to the technical field of genetic engineering, and particularly relates to a CsECT protein related to plant disease resistance, a recombinant vector and application of the CsECT protein. The invention provides a CsECT protein related to plant disease resistance, the CsECT protein is synthesized by gene CsECT coding, it is speculated that the gene CsECT is used as an m6A reader, metabolism of target mRNA of the gene CsECT is affected, the stability of disease resistance related target genes is improved, expression of disease resistance genes is promoted, and then the disease resistance of plants is improved. The result of the embodiment shows that after the CsECT gene is cloned, the CsECT transgenic citrus is constructed, the CsECT gene is excessively expressed by the transgenic citrus, the canker resistance of the citrus can be remarkably improved, and then the disease resistance of the citrus is improved. Therefore, the CsECT protein synthesized by the gene CsECT disclosed by the invention can be used for improving the disease resistance of plants.

The invention discloses Delftia tsuhuensis and an application of the Delftia tsuhuensis in prevention and treatment of plant diseases. The Delftia tsuhuensis CDJ2 is preserved in the Guangdong Microbial Culture Collection Center on October 18, 2024, and the preservation number is GDMCC (China General Microbiological Culture Collection Center) NO: 65290. The strain has a good prevention and treatment effect on citrus canker, and the prevention and treatment effect can reach 84%. Meanwhile, the strain can also effectively prevent and treat soft rot of taro, bacterial basal rot of rice, bacterial wilt of crops, kiwifruit canker, angular leaf spot of mango, black rot of brassicaceous vegetables, tomato scab, gummosis of sugarcane, bacterial wilt of walnuts and bacterial leaf spot of plums. Wide application prospects are realized in the aspects of preventing and treating diseases of crops and commercial crops and promoting healthy growth of plants.

The invention discloses an application of Burkholderia gladioli XC-1 in prevention and treatment of plant diseases, the 16S rRNA sequence of the Burkholderia gladioli XC-1 is as shown in SEQ ID NO: 3, and the plant diseases are litsea cubeba shoot blight, okra root rot, durian leaf spot disease, cubilose fruit canker, agilawood root rot or cassava leaf spot disease. The invention finds that the Burkholderia gladioli XC-1 has better development value and application potential in the prevention and treatment of various plant diseases, and a new microbial strain resource is provided for the prevention and treatment of various plant diseases.

The invention relates to mortierella alpina and application thereof. The strain name is Mortierella alpina RS-01, the preservation number is CGMCC (China General Microbiological Culture Collection Center) No.41944, the preservation time is May 21, 2025, and the preservation location is CGMCC (China General Microbiological Culture Collection Center), No. 3, No.1 yard, West Beichen Road, Chaoyang District, Beijing, China. The mortierella alpina provided by the invention can be used for pathogenic bacteria of ginseng rust rot, pathogenic bacteria of muskmelon fusarium wilt, pathogenic bacteria of pythium aphanidermatum, pathogenic bacteria of apple fruit rot, pathogenic bacteria of northern corn leaf blight, pathogenic bacteria of seedling blight, pathogenic bacteria of walnut canker, pathogenic bacteria of poplar canker, pathogenic bacteria of pepper anthracnose and pathogenic bacteria of blueberry stem canker; the compound has the best antibacterial effect on pathogenic bacteria of apple shell monochromatic alternaria, pathogenic bacteria of tobacco brown spot, and especially pathogenic bacteria of ginseng rust rot.

The invention provides a kiwi fruit transcription factor and application thereof in kiwi fruit canker, and belongs to the technical field of genetic engineering. The invention provides a transcription factor AcWRKY50, the nucleotide sequence of the transcription factor AcWRKY50 is shown as SEQ ID No.1, and the AcWRKY50 is positioned in a cell nucleus and has transcription activation capability. The AcWRKY50 disclosed by the invention can continuously respond to Psa infection, after Psa pathogenic bacteria infection, the expression of the AcWRKY50 gene is rapidly activated and continuously up-regulated, and after the AcWRKY50 gene is over-expressed, the resistance to Psa can be improved. Meanwhile, MeJA negatively regulates the resistance of the kiwi fruit to the Psa, and the kiwi fruit germplasm can be innovated based on the transcription factor disclosed by the invention.

The invention discloses a derivative containing chiral thioether and sulfone units as well as a preparation method and application of the derivative. The derivative containing the chiral thioether and the sulfone unit is applied to preparation of medicaments for preventing and treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, tobacco bacterial wilt, muskmelon bacterial leaf blight, tomato bacterial wilt, watermelon fruit rot, Chinese cabbage soft rot, peach tree shot hole disease and cabbage black rot fungus. The invention further discloses application of the derivative containing the chiral thioether and sulfone unit to preparation of medicaments for preventing and treating rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, tobacco bacterial wilt, muskmelon bacterial leaf blight, tomato bacterial wilt, watermelon fruit rot and cabbage black rot. The derivative containing chiral thioether and sulfone units has the characteristics of broad bactericidal spectrum and high activity, has excellent prevention and treatment effects on bacterial diseases, and is simple and feasible in preparation steps and low in production cost.

The invention belongs to the technical field of genetic engineering, and particularly relates to a citrus canker pathogen mediated citrus leaf transient expression method which comprises the following steps: preparing a citrus canker pathogen infection solution which is xanthomonas citrus subspecies; infecting the citrus by using the citrus canker pathogen infection liquid, and culturing to obtain citrus infected by the citrus canker pathogen; preparing an agrobacterium infection solution containing a target gene; and inoculating the citrus infected by the citrus canker pathogen with an agrobacterium infection solution containing a target gene, and carrying out transient expression of the target gene. The transient expression method provided by the invention can realize transient and efficient expression of the target gene in leaves of different citrus varieties. The transient expression method disclosed by the invention can be used for researching gene functions, protein interaction, promoter activity analysis and the like; and the method can also be used for evaluating the citrus canker resistance gene function.

The invention discloses a Burkholderia cepacia MZ-8 which is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on April 8, 2025, and the preservation number of the Burkholderia cepacia MZ-8 is GDMCC No: 66106. The research finds that the burkholderia cepacia MZ-8 can efficiently inhibit the growth of pathogenic bacteria of citrus canker, the inhibition rate of the burkholderia cepacia MZ-8 to the pathogenic bacteria of citrus canker reaches 98.27% in a pot culture test, and meanwhile, the burkholderia cepacia MZ-8 can dissolve organic phosphorus, generate iron carriers and fix nitrogen and promote plant growth. Therefore, the burkholderia cepacia MZ-8 disclosed by the invention has good application potential in the aspects of preventing and treating citrus canker, promoting absorption and growth of phosphorus, iron and nitrogen of plants and the like. The discovery of the bacterial strain enriches beneficial microbial resources for biocontrol and growth promotion, and provides a bacterial strain which is stable in antibacterial effect, efficient and environment-friendly for green biological control.

The invention relates to the technical field of agricultural biological genes, and discloses a method for improving citrus canker resistance based on a CsKCS2 gene, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsKCS2 gene in citrus plants. The method specifically comprises the following steps: (1) cloning a citrus CsKCS2 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsKCS2 gene expression level. By cloning the coding sequence of the citrus CsKCS2 gene, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsKCS2 overexpression transgenic plant can be reduced to 73.3% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the application prospect is broad. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention belongs to the technical field of genetic engineering, and relates to application of a transcription factor AcWRKY20 in resisting kiwifruit bacterial canker. Through gene cloning, phylogenetic analysis, subcellular localization, transgenic function verification and other technologies, it is proved for the first time that the transcription factor AcWRKY20 has the molecular characteristic of remarkably enhancing the disease resistance of kiwi fruits. According to the invention, the AcWRKY20 gene is respectively silenced and overexpressed and then is inoculated with Psa, and phenotypic observation shows that the gene is a disease-resistant gene and is suitable for canker-resistant breeding of kiwi fruit.

The invention discloses a citrus growth promoting fertilizer and a fertilization method thereof, and relates to the technical field of chemical fertilizer production, the citrus growth promoting fertilizer comprises 40-60 parts of macroelements, 15-25 parts of medium elements, 8-12 parts of trace elements, 10-18 parts of an organic active agent, and 5-10 parts of a slow release agent. Therefore, firstly, the nano calcium carbonate is used for replacing a traditional calcium source, and a polylactic acid-fucoidan slow-release membrane technology is combined, so that the dissolved state keeping time of calcium ions in the soil is prolonged by 30-50%, the calcium absorption rate is increased to 35% or above from the average industrial 20-30%, and the fruit cracking rate of citrus is effectively reduced; the bacillus subtilis and the seaweed extract in the organic active agent have a synergistic effect, through biological antagonism and induced resistance, the canker incidence rate is reduced to 1 / 4 of that of a control group, meanwhile, the soil microenvironment is improved through humic acid, and the root system health degree is improved by 40%.

The invention belongs to the technical field of biological control, and particularly relates to bacillus velezensis JH-25 and application thereof. The invention provides a bacillus velezensis JH-25, and the preservation number of the bacillus velezensis JH-25 is GDMCC No: 65188. The bacillus velezensis JH-25 can be used for preparing bacillus velezensis. The bacillus velezensis JH-25 provided by the invention is obtained by screening konjac tubers as samples, is an endophyte of konjac, and has biocontrol and growth promoting effects. Results of the embodiment show that the bacillus velezensis JH-25 provided by the invention can inhibit colletotrichum gloeosporioides, colletotrichum siamensis, colletotrichum gloeosporioides, corynespora cassiicola, soybean purpura and colletotrichum aceae, prevent and treat various diseases such as anthracnose, brown spot, leaf spot, purpura, stem rot and canker, and promote plant growth.

The application discloses a kiwi bacterial canker disease susceptible gene AcADC1 and application thereof, and belongs to the technical field of genetic engineering. The nucleotide sequence of the AcADC1 gene is shown in SEQ ID NO:1, and the arginine decarboxylase (ADC) synthesized by the gene is encoded, and the amino acid sequence is shown in SEQ ID NO:2, and the arginine (Arg) is responsible for catalyzing the formation of putrescine. The resistance of the kiwi plant with the silenced AcADC1 gene to the bacterial canker disease is improved, the resistance of the kiwi plant with the overexpressed AcADC1 gene to the bacterial canker disease is reduced, the AcADC1 gene plays a negative regulation role in the immune response of the kiwi to the bacterial canker disease, the gene editing technology can be used for editing the AcADC1 gene, and a theoretical basis and gene reserve are provided for kiwi disease-resistant breeding.

The invention relates to the field of pesticide preparations, and discloses a sterilization composition containing tetramycin and Saisentong as well as a preparation method and application of the sterilization composition. Tetramycin and Saisentong serve as active components of the composition, and a dual-structure composite lignin derivative system serves as a functional carrier; the system is composed of high-molecular-weight modified lignosulfonate and specific lignin phenol fragments with low polymerization degree. The high-molecular-weight component forms a physical barrier on the surfaces of active component particles, so that the storage stability and the application safety of the preparation are improved; the low-polymerization-degree component can be degraded by an extracellular enzyme secreted by citrus canker pathogen, so that the targeted release of the active component at the focus is realized. The composition disclosed by the invention effectively reduces the decomposition rate of the active components and the phytotoxicity risk to crops, and meanwhile, the field control effect on the citrus canker is remarkably improved. The composition provides an efficient and safe new choice with a targeting effect for chemical prevention and treatment of citrus canker.

The invention discloses a bactericide containing organic metal salt and a preparation method thereof, the bactericide is generated by chemical reaction of N-phenyl-o-carboxybenzamide and metal inorganic salt, and the preparation method specifically comprises the following steps: adding the metal inorganic salt into water to obtain a metal inorganic salt solution; adding N-phenyl o-carboxybenzamide into the metal inorganic salt solution to obtain a mixture A; heating the mixture A to 55-90 DEG C, keeping the constant temperature for 60-150 minutes, cooling the mixture A to room temperature after the reaction is finished, and then adjusting the pH value of the mixture A to 6-10 to obtain a mixture B; and heating the mixture B to 70-90 DEG C, keeping the constant temperature for 3-5 hours, cooling the mixture B to room temperature after the reaction is finished, filtering, and drying the filtered solid to obtain the bactericide. The bactericide disclosed by the invention not only has good sterilization and control effects on apple tree canker and kiwi fruit canker, but also has a certain regulation effect on plant growth.

The application discloses a pesticide composition containing zhongshengmycin and kanamycin and application thereof, and belongs to the technical field of pesticides. Effective components of the pesticide composition are zhongshengmycin and kanamycin. The mass ratio of the kanamycin to the zhongshengmycin is 1:1-9. The two effective components of the application have different action sites for killing pathogenic bacteria, and can effectively alleviate the generation of drug resistance of the pathogenic bacteria. After the zhongshengmycin and the kanamycin are compounded, synergistic effect is obvious, the control effect of the pesticide on bacterial diseases, such as citrus canker, is improved, the pesticide dosage can be reduced, and agricultural residues are reduced. Meanwhile, the prepared soluble granules do not use any organic solvent, are safe to crops, and have no residues and pollution to the environment.

This invention relates to the field of agricultural pest control, and particularly to a compound nanogel sustained-release agent, its preparation method, and its application. This invention provides a compound nanogel sustained-release agent comprising bromonitol, a synergist, a carrier material, and a cross-linking agent, wherein the synergist includes one or more of kasugamycin, amino oligosaccharides, thiazolium zinc, and copper hydroxide. This invention prepares a nanogel sustained-release agent by compounding bromonitol and the synergist, which has significant preventive effects against plant bacterial diseases such as bacterial wilt, bacterial angular leaf spot, canker, and soft rot. Furthermore, the compound nanogel sustained-release agent of this invention has small particle size, stable physicochemical properties under cold and heat, good dispersibility in water, no particle agglomeration, no particle size increase, a decomposition rate of less than 5%, a long-lasting effect, good safety, is not prone to inducing drug resistance, requires a small dosage, and simultaneously increases yield and efficiency.

The invention discloses bacillus amyloliquefaciens capable of resisting plant pathogenic bacteria and application of the bacillus amyloliquefaciens, and belongs to the technical field of microorganisms, the bacillus amyloliquefaciens is classified and named as bacillus amyloliquefaciens KT-7, the bacillus amyloliquefaciens KT-7 is preserved in Guangdong Microbial Culture Collection Center (GDMCC) on June 9, 2020, and the address is No.66, Courtyard No.100, Xianlie Middle Road, Guangzhou City, Guangdong Province; the preservation number of the strain is GDMCC No: 61051. The bacillus amyloliquefaciens provided by the invention has a growth inhibition effect on plant pathogenic bacteria, and particularly has a good control effect on bacterial diseases such as citrus canker and tomato bacterial wilt.

The invention relates to the technical field of agricultural biological genes, and discloses a CsCRK10 gene, application of encoded protein and a method for improving citrus canker resistance, and the citrus canker resistance of citrus plants is improved by adjusting the expression level of the CsCRK10 gene in the citrus plants; the method specifically comprises the following steps: (1) cloning a citrus CsCRK10 gene sequence; (2) constructing an overexpression vector; and (3) transforming the overexpression vector into citrus to obtain a transgenic material with an up-regulated CsCRK10 gene expression level. By cloning the coding sequence of the CsCRK10 gene of the citrus, constructing an overexpression vector and then transforming the citrus, the canker attack degree of the obtained CsCRK10 overexpression transgenic plant can be reduced to 66.17% of that of the existing citrus to the maximum extent, the canker attack degree can be effectively and remarkably reduced, the scab area is reduced, the phenotype of the transgenic plant is not influenced, and the method is suitable for industrial production. The gene has a great application value for canker-resistant breeding of citrus, and can be used as a candidate gene for canker-resistant breeding with a plurality of canker-resistant and susceptible genes.

The invention relates to the technical field of microbial compositions, in particular to a microbial composition for preventing and treating citrus canker and application of the microbial composition. According to the microbial composition provided by the invention, the pseudomonas Qingdao microbial agent, sodium lignin sulfonate, trehalose, glutathione, ascorbic acid, lecithin and trace elements (Zn, B and Mn) are taken as main raw materials, and the effects of efficiently inhibiting bacteria, remarkably inducing and improving the activity of key defensive enzymes of leaves, promoting synthesis and accumulation of disease-resistant substances and improving the disease resistance of the leaves are achieved through the matching effect of all the components. The diversity and richness of citrus phyllospheric bacterial communities are improved, and the purpose of constructing a stable community dominated by beneficial bacteria is achieved. In other words, the basic stress resistance of the plants can be enhanced, and meanwhile, the growth and metabolism activity of beneficial bacteria is also promoted, so that effective, eco-friendly and sustainable prevention and control of the citrus canker are achieved.

The invention belongs to the technical field of prevention and control of plant bacterial diseases, and particularly relates to an amide derivative containing benzoxazole sulfone and a preparation method and application thereof.The amide compound containing benzoxazole sulfone has good antibacterial activity on plant pathogenic bacteria of rice bacterial leaf blight, rice bacterial streak and citrus canker, and can be used for preventing and controlling bacterial diseases of plants such as rice bacterial leaf blight, rice bacterial streak and citrus canker. The method has the advantages of simple preparation process, excellent reaction yield and low raw material cost; the structural formula of the derivative is shown as I,

The application discloses a primer probe composition which is composed of a forward primer, a reverse primer and a probe primer. The sequence of the forward primer is shown as SEQ ID No. 1, the sequence of the reverse primer is shown as SEQ ID No. 2, and the sequence of the probe is shown as SEQ ID No. 3. The application establishes a detection method for Pseudomonas syringae pv. actidii, which comprises the following steps: extracting genomic DNA of a sample to be detected, taking the genomic DNA as a template, and performing RAA detection by using the primer composition; and determining whether the sample to be detected contains Pseudomonas syringae pv. actidii according to a strip of a LFD test paper. The detection method provided by the application has a minimum detection sensitivity of 1 pg / µL for Pseudomonas syringae pv. actidii DNA, and has a minimum detection limit of 1×10 3 cfu / mL for a bacterial suspension, and can provide a new technical means for early diagnosis and disease prevention and control of bacterial canker disease of kiwifruit.

A new cherry tree particularly distinguished dark red-purple fruit with a kidney to flat-round shape, a precocious fruiting habit, self-fertility, good resistance to rain crack and moderate resistance to bacterial canker, is disclosed.

The present invention relates to the field of agricultural biological gene technology, and discloses a method for improving the resistance of citrus to citrus canker by regulating the expression level of CsSERK1 protein in citrus plants. Specifically, it includes the following steps: (1) cloning the CsSERK1 gene sequence of citrus; (2) constructing an overexpression vector; (3) transforming citrus with the overexpression vector to obtain transgenic plants with up-regulated expression level of CsSERK1 gene. By cloning the coding sequence of the citrus CsSERK1 gene, constructing an overexpression vector, and then transforming citrus, the incidence of citrus canker in the obtained CsSERK1 overexpression transgenic plants can be reduced to 51.74% of that of existing citrus at most, which can effectively and significantly reduce the incidence of citrus canker, reduce the lesion area, and does not affect the phenotype of transgenic plants. It has great application value for citrus canker resistance breeding and can be used as a candidate gene for citrus canker resistance breeding with multiple canker-resistant and -susceptible genes.

The invention discloses a salix psammophila disease and insect pest prevention and control method, and belongs to the technical field of disease and insect pest prevention and control. The prevention and control method specifically comprises the following steps: (1) disease prevention and control: 1, for rot diseases, strengthening maintenance, scraping disease spots and whitewashing tree trunks; 2, for canker, sick branches are cut off, and pesticide prevention and treatment are conducted; (2) pest prevention and control: 1, artificial catching and killing, chemical control and biological control are carried out on salix mongolica moths; 2, light trapping and killing, chemical control and biological control are carried out on gipsy moths; 3, aphids are controlled by pesticides and biologically; through multiple measures such as physical control, chemical control and biological control, occurrence and propagation of diseases and insect pests can be effectively reduced, healthy growth of the salix psammophila is guaranteed, the growth speed of the salix psammophila is increased, the wood quality is improved, and better vegetation conditions are provided for wind prevention, sand fixation and ecological restoration.

The invention discloses a method for preventing and treating kiwifruit canker by using a traditional Chinese medicine fertilizer. According to the method, the occurrence and development of the kiwifruit canker are effectively controlled through disease monitoring and evaluation, agricultural prevention and treatment measures and application of a comprehensive prevention and treatment strategy of matching traditional Chinese medicine fertilizers and other medicaments based on a traditional Chinese medicine agricultural technology. Wherein the traditional Chinese medicine fertilizer takes a Chinese herbal medicine plant-derived traditional Chinese medicine fertilizer with sterilization and bacteriostasis effects as a core, and is precisely applied in different growth stages in combination with a plurality of prevention and treatment medicaments. The application concentration, frequency and opportunity of the traditional Chinese medicine fertilizer and other medicaments as well as disease monitoring and local treatment measures are specified in detail. By implementing the method, the disease resistance and fruit quality of the kiwi fruits can be remarkably improved, meanwhile, the use amount of chemical pesticides is reduced, and the ecological environment is protected. The method has the advantages of being environmentally friendly, efficient, sustainable and the like, and a new technical approach is provided for green prevention and treatment of the kiwifruit canker.

The present application relates to the technical field of pharmaceutical chemistry, and particularly relates to application of tryptophanone in prevention and treatment of plant bacteria and part of plant fungi. The present application discloses that natural product tryptophanone can effectively prevent and treat plant bacterial and part of plant fungal diseases. The application of tryptophanone as an agricultural bactericide in plant bacterial diseases has excellent inhibitory effect on rice bacterial leaf blight, rice bacterial leaf streak, citrus canker, cabbage black rot, tomato bacterial spot, kiwi canker, tobacco bacterial wilt, cucumber bacterial angular spot, potato bacterial wilt, soybean bacterial spot, tomato canker and the like. The tryptophanone also shows good inhibitory activity on plant pathogenic fungi such as grapevine Eutypa lata, tobacco alternaria alternata, eggplant verticillium wilt, pepper fusarium wilt, colletotrichum gloeosporioides, tea tree colletotrichum gloeosporioides and sorghum zylosporium.