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Streptomycete for producing volatile antibacterial substances and application thereof

A bacteriostatic substance, volatile technology, applied in the field of streptomyces, to achieve the effect of reducing severity and simple production process

Active Publication Date: 2018-12-11
GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] It is currently reported that Herbaspirilum spp., Pseudomonass pp., Bacillus amyloliquefaciens, and Trichoderma can produce volatile gases that inhibit the pathogen of Fusarium wilt of banana, but There is no report about the volatile gas produced by Streptomyces inhibiting Fusarium wilt of banana
There is also no report on the inhibition of Fusarium wilt by p-dichlorobenzene

Method used

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  • Streptomycete for producing volatile antibacterial substances and application thereof
  • Streptomycete for producing volatile antibacterial substances and application thereof
  • Streptomycete for producing volatile antibacterial substances and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0051] The isolation of embodiment 1 streptomyces

[0052] In a healthy banana garden in Overseas Chinese Investment Zone, Lijian Town, Wuming County, Nanning City, Guangxi, randomly picked banana rhizosphere soil with a clean shovel and put it in a clean sealed bag. The rhizosphere soil samples were air-dried at 28°C for 4 days. Actinomycetes were isolated by diluting soil plates. According to the ratio of 1:9 (v / w), 10 g of soil samples were weighed and put into 90 ml of sterile 0.85% NaCl solution (at the same time, put the roots with soil on the surface into the flask). Soil samples were repeated three times. All soil suspensions were shaken on a shaker at 25° C., 150 rpm for 30 minutes, and then stood still for 5 minutes. The soil suspension in the upper layer is continuously diluted 10 times with a mass concentration of 0.85% NaCl solution, and the dilution factor is 10 -4 、10 -5 Put 200 μl of the soil suspension on the S medium agar plate, spread it evenly with a s...

Embodiment 2

[0053] The genus and species identification of embodiment 2 streptomyces

[0054] 1. Morphological Identification

[0055] Observation after 5, 7, and 10 days of culture by inserting method: the spore filaments of actinomycete TF78 are in the shape of 2-5 tight spirals, and the spores are spherical and oblong, with smooth or slightly warty surfaces. Actinomycetes TF78 is a white round colony in the early stage on the PDA medium, and the colony in the later stage is grayish yellow (see figure 1 ).

[0056] 2. Observation of culture characteristics

[0057] Actinomycete TF78 was inoculated on glycerol aspartate agar medium, inorganic salt starch agar medium, yeast refined malt extract agar medium, and oat flour medium, and the morphological characteristics were observed, as shown in Table 1.

[0058] Table 1 Culture characteristics of TF78 on different media

[0059] culture medium

aerial mycelium

Substrate mycelium

Soluble pigment

Glycerol Aspart...

Embodiment 3

[0093] The preservation of embodiment 3 streptomyces

[0094] Streptomyces misionensis TF78 was preserved on October 25, 2017 in Guangdong Microbial Culture Collection Center, the address is 5th Floor, Building 59, Compound, No. 100 Xianlie Middle Road, Guangzhou City, and the preservation number is GDMCCNO:60254.

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Abstract

The invention provides streptomycete for producing volatile antibacterial substances and application thereof. The streptomycete is named as Streptomycesmicionensis TF78; the streptomycete was collected to Guangdong Microbiological Culture Collection Center on October 25th, 2017, and the collection number is GDMCC NO:60254. The streptomycete has the inhibiting functions on the pathogenic bacteria of cucumber blight, tomato gray mold, teak canker, banana fungal stem rot, rice blast fungi, mango phomopsis leaf spot, mango pestalotiopsis leaf spot, mango botryosphaeria branch blight, mango anthracnose and the like.

Description

technical field [0001] The invention relates to the field of biotechnology, and discloses a strain of streptomyces producing volatile antibacterial substances and its application. Background technique [0002] The use of volatile substances with antibacterial activity produced by microorganisms to control plant diseases is a new control measure in recent years. These volatile substances are a type of organic and inorganic compounds with small molecular weight, which have good cell membrane penetration and high-efficiency diffusion ability in air and soil gaps, and can quickly expand the scope of action of volatile substances and improve the inhibition of target microorganisms rate (Aochi et al., 2005). In the United States, the endophytic fungus Muscodor albus has been developed as a biofumigant to control Aphanomyces cochlioides, Meloidogyneincognita, P. ulti-mum, Pepper blight (P. capsici), Rhizoctonia solani (R. solani) and Verticillium dahlia (Verticillium dahlia) and ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/02A01N63/00A01P3/00C12R1/465
CPCA01N63/00C12N1/02C12N1/20C12N1/205C12R2001/465
Inventor 黄穗萍莫贱友李其利郭堂勋唐利华
Owner GUANGXI ZHUANG AUTONOMOUS REGION ACAD OF AGRI SCI
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