Bacillus subtilis strain and application thereof

A technology of Bacillus subtilis and strains, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of low yield, fast separation speed, rare strains, etc., and achieve high yield, short fermentation cycle and good effect

Inactive Publication Date: 2018-06-05
北京新正和农业科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, although the existing Bacillus subtilis preparations all have obvious control effects on plant diseases caused by various fungi or bacteria, it is rare to find strains that achieve better results on diseases caused by mixed eubacteria.
[0005] In addition, the existing methods for preparing Bacillus subtilis usually use liquid fermentation to obtain fermented liquid, which is concentrated with a disc centrifuge after pretreatment, and then spray-dried to obtain raw powder; the fermented liquid is separated by a disc centrifuge, and the heavy liquid is left. The light liquid is discarded, the separation speed is fast but the yield is low

Method used

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  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof
  • Bacillus subtilis strain and application thereof

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Embodiment 1

[0033] The present embodiment relates to a kind of preparation method of multifunctional Bacillus subtilis bacterial agent, it comprises the following steps:

[0034] 1), prepare bacterial classification, be about to add 10 Kirschner flask seeds and aseptic water to make bacterial suspension, inoculate in the large tank under aseptic condition;

[0035] 2) Fermentation medium, namely yeast powder with a content of 1.8w / v% and a fineness ≥ 80 meshes, industrial grade cornstarch with a content of 2.0w / v%, industrial grade calcium carbonate with a content of 0.18w / v%, The volume of industrial grade potassium dihydrogen phosphate with a content of 0.88w / v%, industrial grade ammonium sulfate with a content of 0.18w / v%, and industrial grade defoamer with a content of 0.018w / v% after sterilization is the fermenter In a tank with 65% volume, the pH before sterilization is 7.15~7.7; then keep the ventilation volume at 1:0.5 during the period of 0~8h, and keep the ventilation volume at ...

Embodiment 2

[0042] The present embodiment relates to a kind of preparation method of multifunctional Bacillus subtilis bacterial agent, it comprises the following steps:

[0043] 1), prepare bacterial classification, be about to add 10 Kirschner flask seeds and aseptic water to make bacterial suspension, inoculate in the large tank under aseptic condition;

[0044]2) Fermentation medium, namely yeast powder with a content of 2.2w / v% and a fineness ≥ 80 meshes, industrial-grade cornstarch with a content of 1.5w / v%, industrial-grade calcium carbonate with a content of 0.5w / v%, The volume of industrial grade potassium dihydrogen phosphate with a content of 0.24w / v%, industrial grade ammonium sulfate with a content of 0.6w / v%, and industrial grade defoamer with a content of 0.055w / v% after sterilization is the fermenter In a tank with 65% volume, the pH before sterilization is 7.15~7.7; then keep the ventilation volume at 1:0.5 during the period of 0~8h, and keep the ventilation volume at 1:0...

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Abstract

The invention relates to the technical field of microorganism application, in particular to a bacillus subtilis strain and an application thereof. The bacillus subtilis strain is preserved in China General Microbiological Culture Collection Center (CGMCC) on Jan 22rd, 2018 with the preservation number of CGMCC NO.15250. The strain provided by the invention can prevent and treat multiple fungal andbacterial diseases and are applied to Chinese cabbage soft rot, citrus canker, tobacco wildfire, tobacco bacterial wilt and rice bacterial leaf blight at present.

Description

technical field [0001] The invention relates to the technical field of microbial application, in particular to a strain of Bacillus subtilis and its application. Background technique [0002] True and bacterial diseases are two types of major diseases that endanger the growth of crops, and have always seriously endangered agricultural production; at present, the commonly used agents for preventing and controlling true and bacterial diseases are mainly chemical pesticides, and the control effect on some diseases is general. The environment and the quality and safety of agricultural products have a great impact. In my country, there is still a lack of high-efficiency, broad-spectrum, low-toxicity, and low-residue biopesticides mainly for the prevention and treatment of bacterial diseases. [0003] Bacillus subtilis is currently the most effective biocontrol agent for controlling plant diseases. A number of biocontrol strains of Bacillus subtilis have been licensed for commer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P1/00C12R1/125
CPCA01N63/00C12N1/20C12N1/205C12R2001/125
Inventor 易虹
Owner 北京新正和农业科技有限公司
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