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Bacillus amyloliquefaciens K-8 and bactericide thereof

A technology of amylolytic spores and bacilli, applied in the direction of fungicides, bacteria, biocides, etc., can solve the problems of unsatisfactory antibacterial effect and small antibacterial spectrum range, and achieve simple and easy preparation method, environmental friendliness and low cost Effect

Active Publication Date: 2013-06-05
内蒙古永太化学有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microbial pesticides in the prior art have a small range of antibacterial spectrum, and the antibacterial effect is not ideal.

Method used

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  • Bacillus amyloliquefaciens K-8 and bactericide thereof
  • Bacillus amyloliquefaciens K-8 and bactericide thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0023] Embodiment 1 Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Screening and identification of K-8

[0024] This example introduces and understands Bacillus amylobacter ( bacillus amyloliquefaciens ) Isolation, screening and identification of K-8.

[0025] 1. Isolation and screening of antagonistic bacteria of pathogenic bacteria

[0026] In the Badan saline-alkali land of Binhai County, Yancheng City, Jiangsu Province, the diseased plants and surrounding soil were collected for strain isolation and culture. Fungi were isolated using PDA medium and bacteria were isolated using LB medium. A total of 12 fungi and 35 bacteria were isolated. The pathogenic bacteria of tomato bacterial wilt, watermelon wilt and rice sheath blight were used as indicator strains, and were screened by plate confrontation culture method and inhibition zone method. The results are shown in Table 1. From Table 1, it can be seen that strain K-8 has a strong antagonistic effect on the...

Embodiment 2

[0041] Embodiment 2 preparation containing bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Liquid bacterial agent of K-8

[0042] 1. Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Activation of K-8 and preparation of seed solution

[0043] Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) Activation of K-8: from Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 freeze-drying tube, use a sterile inoculation needle to pick part of the bacterial powder into an LB shake flask (250mL shake flask, LB liquid medium filling volume: 50ml), and incubate at 30°C, 150r / min for 24h Afterwards, the LB medium became cloudy. Take the bacterial liquid and put it into the LB solid medium for streak culture for 24h-36h, Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 grows well on LB solid medium, the colony is white or off-white, the surface is irregular and opaque.

[0044] Preparation of the primary seed solution: pick a single co...

Embodiment 3

[0055] Embodiment 3 preparation containing bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 solid bacterial agent

[0056] To the liquid bacterial agent 1 that embodiment 1 makes (viable bacteria content is 30 * 10 8 cfu / mL) with kaolin, the weight ratio of liquid bacterial agent to kaolin is 100:25. The liquid-solid mixture was stirred for 30 minutes and then left to stand for 60 minutes. The solid microbial agent 1 was obtained after the liquid-solid mixture was fed into a centrifugal spray tower by a feeding pump and dried. The drying conditions in the centrifugal spray tower are: the air inlet temperature is 200°C, and the air outlet temperature is 85°C. The water content in the solid microbial agent 1 is 4% (W / W), and the viable bacteria content is 60×10 8 cfu / g.

[0057] Drying conditions must be met: the air inlet temperature is not higher than 250°C, and the air outlet temperature is not higher than 100°C.

[0058] The solid microbial agent must mee...

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Abstract

The invention provides bacillus amyloliquefaciens K-8 and bactericide thereof. The preservation number of the bacillus amyloliquefaciens K-8 strain is CGMCCNO.6486. The bactericide takes the bacillus amyloliquefaciens K-8 strain as an active ingredient. The bacillus amyloliquefaciens K-8 strain is cultured to obtain a liquid bactericide, and a solid bactericide can be obtained by adding a carrier or mixture of the carrier and auxiliaries into the liquid bactericide. The bacillus amyloliquefaciens K-8 strain and the bactericide thereof provided by the invention can be used for effectively preventing and treating diseases such as bacterial wilt, wilt, angular spot, banded sclerotial blight, rice false smut, soft rot, rot, canker and root rot. The bactericide is simple to prepare and easy to use, is applicable to industrialized production and has wide application prospect.

Description

[0001] technical field [0002] The invention relates to the field of microbial pesticides, more specifically to a strain of Bacillus amyloliquefaciens ( bacillus amyloliquefaciens ) K-8 and its cultivation and bacterial preparation. Background technique [0003] In recent years, in agricultural production, diseases such as bacterial wilt, angular leaf spot, canker, soft rot, fusarium wilt, sheath blight and rot have become serious. For these diseases, the current pesticides are safe, effective, In terms of residues, there are certain problems. [0004] With the improvement of people's requirements for environmental protection and food safety, microbial pesticides have received extensive attention. However, the microbial pesticides in the prior art have a small range of antibacterial spectrum and unsatisfactory antibacterial effect. Contents of the invention [0005] The object of the present invention is to provide a kind of bacillus amyloliquefaciens ( bacillus amylo...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/02A01P1/00A01P3/00C12R1/07
Inventor 李际军温学标魏鸿刚赵福兵
Owner 内蒙古永太化学有限公司
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