21 results about "Microbial Biofilms" patented technology

The invention belongs to the technical field of fishpond filtering materials, and discloses a preparation method of a fishpond filtering composite carbon material and the fishpond filtering composite carbon material. The preparation method of the fishpond filtering composite carbon material comprises the following steps: 1) performing kneading and extrusion molding treatment on mixed powder comprising modified bentonite, biomass powder, diatomite, a pore forming agent and a photocatalyst and a binder solution to obtain a green body; 2) drying the green body, performing multi-section programmed heating carbonization in an inert atmosphere, and cooling to obtain a porous carbon ceramic framework loaded with a photocatalyst; 3, the porous carbon ceramic framework is soaked in the graphene oxide dispersion liquid, and the fishpond filtering composite carbon material.The fishpond filtering composite carbon material is obtained.The preparation temperature is relatively low, the fishpond filtering composite carbon material has a developed macropore-mesopore-micropore graded pore structure, and efficient physical adsorption, photocatalytic degradation and microbial biofilm culturing functions can be achieved at the same time.

The invention relates to a microbial biofilm cultivation method comprising the use of a soft agar hydrogel substrate and one or more stains for detection and / or quantification of one or more microbial components and / or characteristics. The invention further relates to the use of the microbial biofilm cultivation method for screening of one or more antimicrobial compounds.

The utility model discloses a bio-trickling filter tank filled with ceramsite filter materials, which comprises a tank body, and the tank body internally comprises a water distribution area, a filler area and a water production area which are separated by a jacking layer and a bearing layer and are communicated with one another from top to bottom; a water inlet pipe and a water outlet pipe of the tank body are respectively communicated with the top and the bottom of the tank body; a self-rotating water distributor connected with the water inlet pipe is mounted in the water distribution area and is used for uniformly distributing sewage entering through the water inlet pipe onto the filler area; the filler area is filled with a ceramsite filter material for microbial biofilm formation and is used for purifying sewage uniformly distributed in the filler area; the water producing area is used for collecting clear water purified by the filler area, and the collected clear water is discharged through a water outlet pipe. The biological trickling filtration tank adopts an integrated structure, the civil engineering cost can be reduced, the ceramsite filter material is light in weight and low in price, the sewage treatment effect is good, rural domestic sewage can be conveniently and movably treated, and the possibility that equipment is idle is reduced.

The invention relates to the technical field of water treatment, and provides a composite material for water treatment, which comprises: a porous base material; the catalyst layer is formed on the surface of the porous base material; the biological affinity layer is formed on the surface of the catalyst layer; the enhancement layer is formed on the surface of the biological affinity layer, and the enhancement layer is graphene quantum dots. The invention further provides a preparation method of the composite material in the technical scheme and water treatment equipment. According to the composite material provided by the invention, through'metal oxide-biological affinity layer-graphene quantum dot 'ternary synergy, deep coupling of ozone oxidation and biodegradation is realized, the ozone removal rate is increased, the microbial biofilm formation rate is increased, the biofilm formation period is shortened to 3-5 days, and the biofilm density is increased by 30%; and the COD (Chemical Oxygen Demand) removal rate is relatively high. Moreover, the composite material provided by the invention is relatively stable, the metal dissolution rate in a high-humidity environment is less than or equal to 1%, and the service life is prolonged to more than 3 years.

The present invention relates to a novel peptide derived from Hirudo nipponia, which has antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity. With antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity, the novel peptide derived from Hirudo nipponia according to the present invention can be advantageously applied as a material for various purposes in the pharmaceutical field, food field, cosmetic field, and agriculture and livestock industries. In addition, the novel antimicrobial peptide derived from Hirudo nipponia according to the present invention increases the sensitivity of antibiotics to multidrug-resistant bacteria, thereby markedly enhancing antimicrobial activity against multidrug-resistant bacteria when used in combination with antibiotics. Accordingly, a composition of the present invention comprising the peptide as an active ingredient can be advantageously used as a pharmaceutical composition for enhancing antibiotic sensitivity. In particular, the novel peptide of the present invention has very low hemolytic activity and cytotoxicity, and thus is safe for the human body. Moreover, the novel peptide of the present invention not only has excellent activity even under a high salt concentration, but also maintains activity even in a serum environment, thereby having high stability when applied in vivo.

This invention belongs to the field of wastewater treatment. It provides a manganese-supported sulfur-rich Spartina alterniflora denitrification material, its preparation method, and its application. This manganese-supported sulfur-rich Spartina alterniflora denitrification material is obtained by alkali-modified sulfur-rich Spartina alterniflora or alkali-modified sulfur-rich Spartina alterniflora biochar loaded with manganese oxides. The manganese-supported sulfur-rich Spartina alterniflora denitrification material of this invention has abundant pores and a large specific surface area, which is beneficial for the adsorption, catalytic oxidation, and microbial biofilm formation of nitrogen pollutants. It is also rich in carbon, sulfur, and manganese, and can directly release organic carbon sources. It can achieve efficient denitrification through multiple processes such as coupled nitrification, manganese ammonium oxidation, heterotrophic denitrification, manganese autotrophic denitrification, and sulfur autotrophic denitrification. The manganese-supported sulfur-rich Spartina alterniflora denitrification material of this invention is fixed on the surface of hard aggregates, elastic or soft carriers, and can be widely used in constructed wetlands, biological filters, deep denitrification in wastewater treatment plants, rural and agricultural wastewater treatment, and initial rainwater purification.

The invention relates to the technical field of bactericides, in particular to an environment-friendly plant-extracted composite sanitizer and application of the environment-friendly plant-extracted composite sanitizer. The traditional Chinese medicine composition comprises the following raw materials: borneol, a pearl derivative, double-petal red rose, ganoderma lucidum, taxus chinensis leaves, a plant source active ingredient extract, sodium salt and purified water. According to the invention, through the synergistic effect of the natural borneol and the pearl derivative, a microbial biofilm barrier is effectively broken through, and efficient and rapid delivery of bactericidal components is realized; intelligent drug loading and directional enrichment characteristics of the nano pearl powder are utilized, and a double-phase protection mechanism combining instant killing and physical blocking is formed; in the whole process, chemical stimulation and environmental pollution are avoided, meanwhile, the broad-spectrum antibacterial advantage of plant active ingredients and the continuous repairing effect of pearl components are perfectly fused, and therefore on the basis of remarkably widening the antibacterial spectrum and improving the sterilization efficiency, multi-target and environment-friendly comprehensive prevention and control over mixed flora infection are achieved.

Micron scale biogeography is a major driver of physiology and ecology of complex microbial biofilm communities, which remains elusive largely due to the lack of tools for spatially resolved phylogenetic mapping. This disclosure provides methods, computer-readable storage devices and kits that allow highly multiplexed and spatially resolved imaging of microbial community spatial organization. The disclosure provides a highly-multiplexed approach to resolve the spatial structure of complex microbial community at high taxonomic resolution.

Laboratory Instrument for Microbial Biofilm Analysis

The invention relates to the technical field of water treatment, and discloses a sewage treatment carbon material with weak surface electronegativity, good hydrophilicity and easy biofilm formation of microorganisms as well as a preparation method and application of the sewage treatment carbon material. The preparation method of the carbon material for sewage treatment comprises the following steps: (1) carbonizing bamboo powder to obtain a carbonized material; (2) carrying out liquid-phase oxidation reaction treatment on the carbonized material, taking out a solid after the reaction is completed, and washing and drying the solid to obtain a carboxylated first precursor; (3) reacting the first precursor with a first solution containing thionyl chloride, taking out a solid after the reaction is completed, and washing and drying the solid to obtain a second precursor subjected to acylating chlorination; and (4) reacting the second precursor with a second solution containing urea, taking out a solid after the reaction is completed, and washing and drying the solid to obtain the amidated carbon material.

The invention discloses a preparation method of a microalgae biofilm microprotein organic water-soluble fertilizer. The preparation method comprises the steps of microalgae biomass pretreatment, deep conversion and enzymolysis, microbial biofilm treatment, feed liquid concentration and blending and by-product treatment. Microalgae biomass is treated through acid-base wall breaking dissolution, advanced oxidation and proteolysis, a microbiological membrane is treated through calcium peroxide heating wall breaking, enzyme (MBE-3) enzymolysis and plate-frame pressure filtration, microprotein feed liquid is obtained and concentrated and blended into an organic water-soluble fertilizer, and by-products are dried to serve as the soil conditioner. According to the method, efficient conversion and full-value utilization of microalgae and microbial biological membrane resources are achieved, the protein extraction rate and the product fertilizer efficiency are improved, the process integration is high, and the ecological and economic benefits are remarkable.

To provide compositions and methods for preventing, inhibiting, disrupting, or treating polymicrobial biofilms.SOLUTION: For example, methods are provided for one or more of the following: preventing, inhibiting, disrupting, dispersing, or treating invitro and / or invivo a polymicrobial biofilm comprising Haemophilus bacteria in a subject in need thereof. The method comprises or consists essentially of or yet further consists of contacting a multi-microbial biofilm with (i) an anti-DNA binding and bending protein (DNABII) antibody or a biologically active fragment thereof and (ii) an anti-type IV pilin (T4P) majority subunit (PII A) antibody or a biologically active fragment thereof.SELECTED DRAWING: None

The application discloses a novel combined suspended ball filler for simultaneous nitrogen and phosphorus removal in a biochemical aerobic section, which is filled into a porous suspended ball by a mixed material composed of a composite sulfur component filler and a porous sponge. The novel combined suspended ball filler can be used in the biochemical aerobic section, can enrich the microbial biofilm on one hand, and can successfully remove nitrate nitrogen by increasing the composite sulfur component filler on the other hand, so that simultaneous nitrification and denitrification is achieved, nitrate nitrogen and ammonia nitrogen are removed at the same time, the nitrogen and phosphorus removal effect is improved, and the proportion of the composite sulfur component filler and the porous sponge can be flexibly adjusted according to different water quality conditions, so that efficient nitrogen and phosphorus removal can be realized in different wastewaters.

The application discloses a low-carbon and high-efficiency seawater breeding wastewater treatment device and method, wherein the device comprises a sequencing batch reactor and a modified hollow fiber membrane assembly arranged in the sequencing batch reactor, and is provided with a stirring, constant temperature, monitoring and aeration control unit. The method strengthens the microbial biofilm by the modified membrane assembly, adopts a salinity and antibiotic gradient domestication strategy, constructs a stable simultaneous nitrification and denitrification (SND) process under a low dissolved oxygen (DO<0.2 mg / L) condition, and realizes efficient and collaborative removal of carbon, nitrogen pollutants and antibiotics. The application significantly improves the stability of the system against salinity and antibiotic impact, reduces the aeration energy consumption, effectively solves the problem that the microbial activity is inhibited when the traditional biological process is used to treat high-salinity and antibiotic-containing seawater breeding wastewater, and provides a reliable technical scheme for low-carbon and high-efficiency treatment of seawater breeding wastewater.

Antimicrobial azo compounds include azobenzene monomers having a phenyl ring comprising a hydroxyl substituent group. The azobenzene monomers can be reacted with a polymeric material to form an antimicrobial coating that prevents growth of persistent biofilms. In some implementations, the azobenzene monomers can be used to coat the surface of a dental resin-based composite to prevent the formation secondary caries. In some implementations, the azobenzene coating can be used to disperse microbial biofilms through a photofluidization process (rapid photoisomerization) in addition to biochemically preventing formation of the microbial biofilm.

The present application relates to the technical field of water treatment, and discloses a high-biological-affinity carbon material with weak surface electronegativity, good hydrophilicity and easy microbial biofilm formation, and a preparation method and application thereof.The preparation method comprises the following steps: (1) loading iron salt on biomass powder to obtain a first precursor; (2) adding the first precursor into a gel solution containing sodium alginate, urea, phytic acid and thiourea to obtain a second precursor; (3) adding the second precursor dropwise into a calcium chloride solution for solidification to obtain a third precursor; (4) performing freeze-drying treatment on the third precursor to obtain a fourth precursor; (5) performing heat treatment on the fourth precursor in an inert atmosphere to obtain a fifth precursor; (6) performing surface oxidation treatment on the fifth precursor to obtain a sixth precursor; (7) adding the sixth precursor into a MES buffer solution and reacting with EDC and NHS to obtain a seventh precursor; and (8) adding a chitosan-acetic acid mixed solution to the seventh precursor to obtain the high-biological-affinity carbon material.

This utility model discloses a microbial biofilm attachment device that is easy to clean, comprising a cylindrical body, a fixed frame fixedly connected to the outer side of the cylindrical body, an inspection door on the outer side of the cylindrical body, and a rotating shaft rotatably connected to the bottom of the cylindrical body via bearings. Two slides are fixedly connected to the outer side of the rotating shaft, and each of the two slides has a groove at both ends. Two first slides and a second slide are slidably connected within the grooves. A biological carrier is provided on one side of each of the first and second slides, and guide rods are fixedly connected to both sides of the second slide. This utility model not only facilitates the disassembly and cleaning of the biological carrier, increasing cleaning efficiency, but also promotes the growth of heterotrophic bacteria by expanding the spacing and strengthens the dominant nitrifying bacteria population by compressing the spacing, increasing the applicability of the device. Furthermore, the rotating shaft is rotated by the water pump's suction force, further enhancing the device's convenience.

The present application relates to oil and gas field microbial water treatment technical field, specifically, it relates to a kind of microbial water treatment scale removal device;Including reaction device shell, the reaction device shell is equipped with water inlet and water outlet, the reaction device shell is divided into first aeration zone, electric descaling area and second aeration zone three parts, the first aeration zone, the electric descaling area and the second aeration zone are sequentially arranged from the water inlet end to the water outlet end, the electric descaling area inside is equipped with at least one electrode group, and the second aeration zone inside is equipped with at least one microbial biofilm;First through the first aeration zone to the reducing ion in liquid oxidation, deposition to the lower electric descaling area, electric descaling area is adsorbed to the scale-forming ion in liquid, so that effectively reduce the scale-forming ion amount in liquid through the surface of microbial biofilm, effectively avoid microbial biofilm due to surface fouling and affect water treatment effect, effectively improve water treatment quality, ensure that water injection water quality is stable.

The invention is directed to a process to convert carbon dioxide to methane by contacting an aqueous solution comprising dissolved carbon dioxide with an electron charged packed bed comprising of a carrier, suitably activated carbon granules, and a biofilm of microorganisms under anaerobic conditions, wherein more than 90 mol % of the dissolved carbon dioxide in the aqueous solution is present as a bicarbonate ion and / or as a carbonate ion.

The present invention provides compositions comprising a polyphosphate and an amino acid N, N-diacetic acid, formulated as either liquid compositions that upon warming to body temperature solidify into a viscous gel; or solid dosage forms, which are useful as dental compositions for removing microbial biofilm, or inhibiting or disrupting formation thereof.

The application belongs to the technical field of fish pond filtering materials, and discloses a preparation method of fish pond filtering composite carbon material and the fish pond filtering composite carbon material. The preparation method of the fish pond filtering composite carbon material comprises the following steps: 1) mixing powders including modified bentonite, biomass powder, diatomite, pore-forming agent and photocatalyst with a binder solution, and performing kneading and extrusion molding treatment to obtain a green body; 2) drying the green body, and performing multi-stage programmed temperature rising carbonization under an inert atmosphere to obtain a porous carbon ceramic framework loaded with photocatalyst after cooling; and 3) immersing the porous carbon ceramic framework in a graphene oxide dispersion solution, and performing reduction and solidification treatment to obtain the fish pond filtering composite carbon material. The preparation temperature is relatively low, and the fish pond filtering composite carbon material has a developed macropore-mesopore-micropore hierarchical pore structure, and can simultaneously realize efficient physical adsorption, photocatalytic degradation and microbial biofilm formation functions.