48 results about "Microbial Biofilms" patented technology

The invention belongs to the technical field of fishpond filtering materials, and discloses a preparation method of a fishpond filtering composite carbon material and the fishpond filtering composite carbon material. The preparation method of the fishpond filtering composite carbon material comprises the following steps: 1) performing kneading and extrusion molding treatment on mixed powder comprising modified bentonite, biomass powder, diatomite, a pore forming agent and a photocatalyst and a binder solution to obtain a green body; 2) drying the green body, performing multi-section programmed heating carbonization in an inert atmosphere, and cooling to obtain a porous carbon ceramic framework loaded with a photocatalyst; 3, the porous carbon ceramic framework is soaked in the graphene oxide dispersion liquid, and the fishpond filtering composite carbon material.The fishpond filtering composite carbon material is obtained.The preparation temperature is relatively low, the fishpond filtering composite carbon material has a developed macropore-mesopore-micropore graded pore structure, and efficient physical adsorption, photocatalytic degradation and microbial biofilm culturing functions can be achieved at the same time.

The invention relates to a microbial biofilm cultivation method comprising the use of a soft agar hydrogel substrate and one or more stains for detection and / or quantification of one or more microbial components and / or characteristics. The invention further relates to the use of the microbial biofilm cultivation method for screening of one or more antimicrobial compounds.

The utility model discloses a bio-trickling filter tank filled with ceramsite filter materials, which comprises a tank body, and the tank body internally comprises a water distribution area, a filler area and a water production area which are separated by a jacking layer and a bearing layer and are communicated with one another from top to bottom; a water inlet pipe and a water outlet pipe of the tank body are respectively communicated with the top and the bottom of the tank body; a self-rotating water distributor connected with the water inlet pipe is mounted in the water distribution area and is used for uniformly distributing sewage entering through the water inlet pipe onto the filler area; the filler area is filled with a ceramsite filter material for microbial biofilm formation and is used for purifying sewage uniformly distributed in the filler area; the water producing area is used for collecting clear water purified by the filler area, and the collected clear water is discharged through a water outlet pipe. The biological trickling filtration tank adopts an integrated structure, the civil engineering cost can be reduced, the ceramsite filter material is light in weight and low in price, the sewage treatment effect is good, rural domestic sewage can be conveniently and movably treated, and the possibility that equipment is idle is reduced.

Micron scale biogeography is a major driver of physiology and ecology of complex microbial biofilm communities, which remains elusive largely due to the lack of tools for spatially resolved phylogenetic mapping. This disclosure provides methods, computer-readable storage devices and kits that allow highly multiplexed and spatially resolved imaging of microbial community spatial organization. The disclosure provides a highly-multiplexed approach to resolve the spatial structure of complex microbial community at high taxonomic resolution.

The invention relates to the technical field of water treatment, and provides a composite material for water treatment, which comprises: a porous base material; the catalyst layer is formed on the surface of the porous base material; the biological affinity layer is formed on the surface of the catalyst layer; the enhancement layer is formed on the surface of the biological affinity layer, and the enhancement layer is graphene quantum dots. The invention further provides a preparation method of the composite material in the technical scheme and water treatment equipment. According to the composite material provided by the invention, through'metal oxide-biological affinity layer-graphene quantum dot 'ternary synergy, deep coupling of ozone oxidation and biodegradation is realized, the ozone removal rate is increased, the microbial biofilm formation rate is increased, the biofilm formation period is shortened to 3-5 days, and the biofilm density is increased by 30%; and the COD (Chemical Oxygen Demand) removal rate is relatively high. Moreover, the composite material provided by the invention is relatively stable, the metal dissolution rate in a high-humidity environment is less than or equal to 1%, and the service life is prolonged to more than 3 years.

The present invention relates to a novel peptide derived from Hirudo nipponia, which has antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity. With antimicrobial activity, antibiofilm activity, antibiotic sensitivity–enhancing activity, and anti-inflammatory activity, the novel peptide derived from Hirudo nipponia according to the present invention can be advantageously applied as a material for various purposes in the pharmaceutical field, food field, cosmetic field, and agriculture and livestock industries. In addition, the novel antimicrobial peptide derived from Hirudo nipponia according to the present invention increases the sensitivity of antibiotics to multidrug-resistant bacteria, thereby markedly enhancing antimicrobial activity against multidrug-resistant bacteria when used in combination with antibiotics. Accordingly, a composition of the present invention comprising the peptide as an active ingredient can be advantageously used as a pharmaceutical composition for enhancing antibiotic sensitivity. In particular, the novel peptide of the present invention has very low hemolytic activity and cytotoxicity, and thus is safe for the human body. Moreover, the novel peptide of the present invention not only has excellent activity even under a high salt concentration, but also maintains activity even in a serum environment, thereby having high stability when applied in vivo.

The utility model provides spherical MBBR (Moving Bed Biofilm Reactor) filler, a lower shell comprises a spindle-shaped hollow placing cavity, the bottom end of the placing cavity is closed, the top end of the placing cavity is open, and the top end of the placing cavity is connected with a connecting ring A; arc-shaped fins A are arranged on the outer side face of the containing cavity, the top faces of the fins A are connected with the bottom face of the connecting ring A, and the bottom ends of the fins A are connected with the bottom face of the containing cavity; the sealing cover of the upper shell is connected with the inner side surface of the connecting ring B; the bottom surfaces of the fins B on the sealing cover are planes; the bottom surface of the fin B is connected with the sealing cover and the top surface of the connecting ring B; when the upper shell body and the lower shell body are connected into a whole, the sealing cover seals the top end of the containing cavity, and the outer edges of the arc-shaped fins B and the outer edges of the arc-shaped fins A form discontinuous arcs. Small activated carbon balls are arranged in the placement cavity, and the particle size of the small activated carbon balls is 5-8 mm. The spherical MBBR filler is suitable for microbial growth and biofilm formation, and has a good fluidization effect and a stable structure in sewage treatment.

The present invention consists of the use of cyclic oligosaccharides as modulators of the activity of antiseptic active ingredients, causing a synergistic effect in antimicrobial compositions; more specifically, the present invention presents the ability of cyclic oligosaccharides to alter or modulate the spectrum of antimicrobial activity of an antiseptic active ingredient, enabling efficacy against microorganisms against which the active ingredient is not effective in its isolated form or increasing its efficacy against fungi, resistant microorganisms, such as resistant fungi, and microorganism biofilms. The present invention also presents the novelty of a composition that hinders the development of resistant microorganisms and / or eliminates resistant microorganisms, thanks to the specific characteristic of the cyclic oligosaccharide and the slow and continuous release of the antiseptic active ingredient. For resistant microorganism biofilms, the prolonged antiseptic action obtained has a significant impact on combating the complex defences created by biofilms.

This invention belongs to the field of wastewater treatment. It provides a manganese-supported sulfur-rich Spartina alterniflora denitrification material, its preparation method, and its application. This manganese-supported sulfur-rich Spartina alterniflora denitrification material is obtained by alkali-modified sulfur-rich Spartina alterniflora or alkali-modified sulfur-rich Spartina alterniflora biochar loaded with manganese oxides. The manganese-supported sulfur-rich Spartina alterniflora denitrification material of this invention has abundant pores and a large specific surface area, which is beneficial for the adsorption, catalytic oxidation, and microbial biofilm formation of nitrogen pollutants. It is also rich in carbon, sulfur, and manganese, and can directly release organic carbon sources. It can achieve efficient denitrification through multiple processes such as coupled nitrification, manganese ammonium oxidation, heterotrophic denitrification, manganese autotrophic denitrification, and sulfur autotrophic denitrification. The manganese-supported sulfur-rich Spartina alterniflora denitrification material of this invention is fixed on the surface of hard aggregates, elastic or soft carriers, and can be widely used in constructed wetlands, biological filters, deep denitrification in wastewater treatment plants, rural and agricultural wastewater treatment, and initial rainwater purification.

The invention relates to the technical field of bactericides, in particular to an environment-friendly plant-extracted composite sanitizer and application of the environment-friendly plant-extracted composite sanitizer. The traditional Chinese medicine composition comprises the following raw materials: borneol, a pearl derivative, double-petal red rose, ganoderma lucidum, taxus chinensis leaves, a plant source active ingredient extract, sodium salt and purified water. According to the invention, through the synergistic effect of the natural borneol and the pearl derivative, a microbial biofilm barrier is effectively broken through, and efficient and rapid delivery of bactericidal components is realized; intelligent drug loading and directional enrichment characteristics of the nano pearl powder are utilized, and a double-phase protection mechanism combining instant killing and physical blocking is formed; in the whole process, chemical stimulation and environmental pollution are avoided, meanwhile, the broad-spectrum antibacterial advantage of plant active ingredients and the continuous repairing effect of pearl components are perfectly fused, and therefore on the basis of remarkably widening the antibacterial spectrum and improving the sterilization efficiency, multi-target and environment-friendly comprehensive prevention and control over mixed flora infection are achieved.

The utility model discloses a photocatalytic biological rotating disc processor suitable for black and odorous water, which comprises a support, a biological rotating disc mechanism, a first cleaning mechanism and a second cleaning mechanism, and the biological rotating disc mechanism comprises a rotating disc shaft, a plurality of first discs and a plurality of second discs rotatably arranged on the rotating disc shaft, and a rotating disc driving motor in transmission connection with the rotating disc shaft, the first disc and the second disc are arranged at intervals, titanium dioxide photocatalysts are loaded on the two sides of the first disc, and microorganism biofilm culturing areas are arranged on the two sides of the second disc. According to the invention, photocatalysis, biodegradation and the solar photovoltaic panel are combined, organic pollutants in the black and odorous water body can be effectively treated, and energy consumption is reduced by using solar energy.

Micron scale biogeography is a major driver of physiology and ecology of complex microbial biofilm communities, which remains elusive largely due to the lack of tools for spatially resolved phylogenetic mapping. This disclosure provides methods, computer-readable storage devices and kits that allow highly multiplexed and spatially resolved imaging of microbial community spatial organization. The disclosure provides a highly-multiplexed approach to resolve the spatial structure of complex microbial community at high taxonomic resolution.

Disclosed are compositions comprising Rhamnus prinoides (GESHO) extract or fragments thereof and methods of using Gesho extract for the inhibition of biofilm formation.

Peptide nucleic acids, compositions containing the peptide nucleic acids, and methods utilizing the peptide nucleic acids are described. The peptide nucleic acids, compositions, and methods are designed to inhibit global regulatory bacterial genes and useful for preventing biofilm formation on biotic and abiotic surfaces. The PNAs are designed to account for nuanced interspecies variations in these regulatory genes, thus allowing for simultaneous inhibition of multiple bacterial species as well as polymicrobial biofilms. The disclosure outlines the composition of wide range PNAs (wrPNAs) and applications for delivery.

The application discloses a combined process and system for ecological restoration of a non-point source pollution lake, comprising a pump station unit, a slope protection and phosphorus removal unit, a river and lake purifier unit and a benthic habitat restoration unit. The pump station unit comprises a water diversion line and a pump station. The water diversion line is arranged along the banks of farmlands, green lands and villages. A water lifting pump station is arranged to lift the water to the upstream of the river and lake. The ecological phosphorus removal slope protection unit and the benthic habitat restoration unit are composed of porous ecological blocks. The units can effectively remove various pollutants such as TP, COD and ammonia nitrogen in the water and the bottom mud through various treatment methods such as adsorption, attraction, chemical precipitation, ion exchange and biological degradation. The continuous porous structure of the porous ecological blocks is suitable for the growth and extension of plant root systems and the growth and enrichment of microbial biofilm, and is beneficial to the ecological restoration and habitat construction of the bank slope and the water bottom. The floating frame made of a water quality purification carrier and the suspended built-in carrier filler are not only beautiful but also can effectively purify the water quality.

Laboratory Instrument for Microbial Biofilm Analysis

The invention relates to the technical field of water treatment, and discloses a sewage treatment carbon material with weak surface electronegativity, good hydrophilicity and easy biofilm formation of microorganisms as well as a preparation method and application of the sewage treatment carbon material. The preparation method of the carbon material for sewage treatment comprises the following steps: (1) carbonizing bamboo powder to obtain a carbonized material; (2) carrying out liquid-phase oxidation reaction treatment on the carbonized material, taking out a solid after the reaction is completed, and washing and drying the solid to obtain a carboxylated first precursor; (3) reacting the first precursor with a first solution containing thionyl chloride, taking out a solid after the reaction is completed, and washing and drying the solid to obtain a second precursor subjected to acylating chlorination; and (4) reacting the second precursor with a second solution containing urea, taking out a solid after the reaction is completed, and washing and drying the solid to obtain the amidated carbon material.

The invention discloses a preparation method of a microalgae biofilm microprotein organic water-soluble fertilizer. The preparation method comprises the steps of microalgae biomass pretreatment, deep conversion and enzymolysis, microbial biofilm treatment, feed liquid concentration and blending and by-product treatment. Microalgae biomass is treated through acid-base wall breaking dissolution, advanced oxidation and proteolysis, a microbiological membrane is treated through calcium peroxide heating wall breaking, enzyme (MBE-3) enzymolysis and plate-frame pressure filtration, microprotein feed liquid is obtained and concentrated and blended into an organic water-soluble fertilizer, and by-products are dried to serve as the soil conditioner. According to the method, efficient conversion and full-value utilization of microalgae and microbial biological membrane resources are achieved, the protein extraction rate and the product fertilizer efficiency are improved, the process integration is high, and the ecological and economic benefits are remarkable.

To provide compositions and methods for preventing, inhibiting, disrupting, or treating polymicrobial biofilms.SOLUTION: For example, methods are provided for one or more of the following: preventing, inhibiting, disrupting, dispersing, or treating invitro and / or invivo a polymicrobial biofilm comprising Haemophilus bacteria in a subject in need thereof. The method comprises or consists essentially of or yet further consists of contacting a multi-microbial biofilm with (i) an anti-DNA binding and bending protein (DNABII) antibody or a biologically active fragment thereof and (ii) an anti-type IV pilin (T4P) majority subunit (PII A) antibody or a biologically active fragment thereof.SELECTED DRAWING: None

The application discloses a novel combined suspended ball filler for simultaneous nitrogen and phosphorus removal in a biochemical aerobic section, which is filled into a porous suspended ball by a mixed material composed of a composite sulfur component filler and a porous sponge. The novel combined suspended ball filler can be used in the biochemical aerobic section, can enrich the microbial biofilm on one hand, and can successfully remove nitrate nitrogen by increasing the composite sulfur component filler on the other hand, so that simultaneous nitrification and denitrification is achieved, nitrate nitrogen and ammonia nitrogen are removed at the same time, the nitrogen and phosphorus removal effect is improved, and the proportion of the composite sulfur component filler and the porous sponge can be flexibly adjusted according to different water quality conditions, so that efficient nitrogen and phosphorus removal can be realized in different wastewaters.

The application discloses a low-carbon and high-efficiency seawater breeding wastewater treatment device and method, wherein the device comprises a sequencing batch reactor and a modified hollow fiber membrane assembly arranged in the sequencing batch reactor, and is provided with a stirring, constant temperature, monitoring and aeration control unit. The method strengthens the microbial biofilm by the modified membrane assembly, adopts a salinity and antibiotic gradient domestication strategy, constructs a stable simultaneous nitrification and denitrification (SND) process under a low dissolved oxygen (DO<0.2 mg / L) condition, and realizes efficient and collaborative removal of carbon, nitrogen pollutants and antibiotics. The application significantly improves the stability of the system against salinity and antibiotic impact, reduces the aeration energy consumption, effectively solves the problem that the microbial activity is inhibited when the traditional biological process is used to treat high-salinity and antibiotic-containing seawater breeding wastewater, and provides a reliable technical scheme for low-carbon and high-efficiency treatment of seawater breeding wastewater.

The application belongs to the technical field of sewage treatment and specifically relates to a MBBR carrier biofilm formation process, which comprises the following steps: S1, using a low-temperature plasma reaction gas to treat a MBBR carrier to obtain a modified MBBR carrier; S2, inoculating aerobic nitrifying bacteria in a reactor loaded with sewage to make the aerobic nitrifying bacteria expand and culture, and obtaining a bacterial solution; S3, adding the modified MBBR carrier and N-acyl homoserine lactone into the bacterial solution to make the modified MBBR carrier surface preliminarily form a biofilm, and obtaining a first biofilm formation system; and S4, inoculating anaerobic denitrifying bacteria into the first biofilm formation system to make the modified MBBR carrier surface form a biofilm, and obtaining a second biofilm formation system. Through the process of low-temperature plasma modification, signal factor introduction, special biofilm formation sequence and the cooperation thereof, the multiple stages of microbial biofilm formation are considered, the connection strength between the biofilm and the surface of the MBBR carrier can be strengthened while the biofilm formation process is shortened, and the sewage ammonia nitrogen treatment effect is good.

The present invention relates to a waste gas purification method based on nutrient slow-release biological filler, comprising the following steps: 1. preparing nutrient slow-release biological filler; 2. preparing modified polyurethane sponge filler; 3. configuring culture nutrient solution and domestication nutrient solution, and cultivating and domesticating activated sludge in a bioreactor; 4. loading the nutrient slow-release biological filler of step 1 and the modified polyurethane sponge filler of step 2 into a biotrickling filter as fillers of a packing layer; 5. injecting the activated sludge domesticated in step 3 into the biotrickling filter, and forming microbial biofilms on the filler surface; 6. dripping the domestication nutrient solution of step 3 into the top of the biotrickling filter and flowing it out from the bottom of the filter to realize the circulation of the domestication nutrient solution; 7. exhaust gas enters the bottom of the biotrickling filter and is discharged from the top of the filter after being treated by the packing layer. The method of the present invention has the characteristics of high purification efficiency, strong system stability, energy saving and carbon reduction, and environmental friendliness, and has stronger adaptability under different working conditions.

The invention relates to the technical field of sewage treatment, in particular to a synchronous short-cut nitrification-denitrification sewage treatment method based on strain culture, which comprises the following steps: (1) respectively inoculating short-cut nitrifying bacteria and denitrifying bacteria into a liquid culture medium A and a liquid culture medium B, and carrying out strain amplification culture to obtain a short-cut nitrifying bacteria solution and a denitrifying bacteria solution; the short-cut nitrifying bacteria comprise air bacillus E4 of which the preservation number is CCTCC No: M 2025641; (2) respectively introducing a short-cut nitrification bacterium solution and a denitrification bacterium solution into a short-cut nitrification device and a denitrification device, and carrying out microbial biofilm formation; and (3) introducing to-be-treated sewage into the short-cut nitrification device for short-cut nitrification treatment, then introducing the to-be-treated sewage into the denitrification device for denitrification treatment, synchronously performing the step (1) during the period, and respectively supplementing the short-cut nitrification bacterium liquid and the denitrification bacterium liquid into the short-cut nitrification device and the denitrification device. According to the method, the strain amplification culture and the short-cut nitrification-denitrification treatment process of the sewage are synchronously carried out, and the new short-cut nitrification strain is matched, so that the strain demand quantity can be reduced, and the sewage treatment effect is improved.

The invention discloses a low-maintenance chemical-agent-free organic matter and total nitrogen online monitoring system and method, and relates to the technical field of water organic matter and total nitrogen monitoring. The organic matter and total nitrogen on-line monitoring system provided by the invention comprises a membrane assembly, a biological membrane carrier, a pressure gauge, a water pump and a monitoring unit. Water body particles are intercepted through the membrane assembly, and light scattering interference of the particles is eliminated; ammonia nitrogen is oxidized into nitrate nitrogen by utilizing a membrane assembly and a microbial biological membrane loaded on the surface of a biological membrane carrier, so that total nitrogen non-chemical digestion measurement is realized; the monitoring unit detects the concentrations of organic matters (containing various fluorescent organic matters) and total nitrogen (in the form of nitrate nitrogen). The system further comprises a communication control unit for realizing real-time transmission of data and remote regulation and control of membrane flux; meanwhile, the membrane pollution maintenance period is greatly prolonged by virtue of the operation and degradation capabilities of thalli in the biological membrane; no chemical reagent is needed, organic matter and total nitrogen concentration data can be obtained in real time, and the method is suitable for in-situ online water quality monitoring of lakes, discharge points and other scenes.

The present invention discloses a water treatment device of an MABR membrane aeration bioreactor and a treatment method thereof, which relate to the field of sewage treatment. The water treatment device comprises a vertical frame body, wherein oxygen permeable membranes are uniformly arranged inside the vertical frame body, and connecting pipes are arranged at both front and rear ends of the oxygen permeable membrane. A delivery pipe is fixedly connected to the surface of the connecting pipe at the rear end, and the connecting pipe is used to deliver oxygen to the oxygen permeable membrane. The connecting pipe is connected to the vertical frame body through a motion component. Through the dual-mode switching of the motion component, the oxygen permeable membrane is driven to perform cyclic twisting and swinging, thereby enhancing the contact between the surface of the oxygen permeable membrane and the microbial biofilm, improving the oxygen utilization rate, dynamically adjusting the motion state of the membrane, and enhancing the self-cleaning ability of the membrane. The acceleration mechanism continuously swings at the bottom of the oxygen permeable membrane, accelerating the contact between the oxygen permeable membrane and pollutants in water, while digesting the sludge deposited on the outer surface of the oxygen permeable membrane.

The invention provides dynamic bacteriostatic equipment for sludge treatment, which comprises a frame body and a storage bin mounted on the frame body, the storage bin comprises a shell inner layer, a shell middle layer and a shell outer layer, a plurality of connecting parts are arranged between the shell inner layer and the shell middle layer, and the shell inner layer and the shell middle layer are divided into a plurality of groups of air passages by the connecting parts. Multi-dimensional bacteriostasis is achieved through the synergistic effect of a low-temperature environment, a nitrogen environment and ultrasonic waves, the semiconductor chilling plate set, the temperature sensor and the PID temperature controller are matched, the temperature in the storage bin is controlled to be 4 + / -1 DEG C, protein synthesis of mesophilic bacteria is effectively inhibited, the reproduction rate of the mesophilic bacteria is reduced, nitrogen is sprayed out of micropores in the bin wall to form a dynamic gas film layer, a low-oxygen environment is created, and the low-oxygen environment is improved. And low-frequency ultrasonic waves generated by the ultrasonic transducer destroy a microbial biofilm structure and interfere flora aggregation by utilizing a cavitation effect, so that the sludge fermentation decay is comprehensively delayed, and the sludge storage quality is improved.

Antimicrobial azo compounds include azobenzene monomers having a phenyl ring comprising a hydroxyl substituent group. The azobenzene monomers can be reacted with a polymeric material to form an antimicrobial coating that prevents growth of persistent biofilms. In some implementations, the azobenzene monomers can be used to coat the surface of a dental resin-based composite to prevent the formation secondary caries. In some implementations, the azobenzene coating can be used to disperse microbial biofilms through a photofluidization process (rapid photoisomerization) in addition to biochemically preventing formation of the microbial biofilm.

A biocompatible antimicrobial composition effective against microbial biofilms, including in vivo biofilms containing gram-positive bacteria, can be delivered to and used near the spinal column of vertebrates. Once introduced to an area colonized with a bacterial biofilm, particularly an intervertebral disc, the antimicrobial composition weakens the EPS / ECPS macromolecules of the biofilm, which makes the entire biofilm structure susceptible to being dislodged from the affected area or biosorbed. Additionally or alternatively, certain of the subcomponents of the solute component, as well as perhaps the organic liquid(s) of the solvent component, might lyse microbes, particularly gram-positive bacteria, previously protected by the EPS / ECPS macromolecules. Swelling of and around previously impacted intervertebral discs can be reduced or eliminated, often resulting in appreciable reductions in patient pain levels.

The invention belongs to the technical field of new materials, pollution treatment and microorganisms, and particularly relates to an iron mineral-microorganism biological membrane, a preparation method thereof and application of the iron mineral-microorganism biological membrane in degradation of pollutants in a dark environment. The iron mineral-microbial biological membrane provided by the invention comprises a lamellar biological membrane formed by bacillus megatherium and iron minerals dispersed in the biological membrane. The iron mineral-microbial biological membrane converts photo-induced electrons into stable chemical energy to be stored, electrons are continuously released in a dark environment, and efficient degradation of pollutants in the dark environment is achieved.