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Burkholderia pyrrocinia and application thereof in control of dothiorella gregaria

A technology of Burkholderia pyrrole and poplar canker, applied in the field of Burkholderia pyrrole and its application in the prevention and treatment of poplar canker, achieving a large strain database and good development and application Prospects, effects of ease of industrial production

Active Publication Date: 2009-10-14
NANJING FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But using Bc as material to prevent and treat poplar canker has not been reported

Method used

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  • Burkholderia pyrrocinia and application thereof in control of dothiorella gregaria
  • Burkholderia pyrrocinia and application thereof in control of dothiorella gregaria
  • Burkholderia pyrrocinia and application thereof in control of dothiorella gregaria

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Embodiment 1: Burkholderia pyrrole JK-SH007 is to poplar canker pathogen Cytosporachrysosperma, Phomnopsis sp., Fusicoccum aesculi Plate antibacterial effect.

[0037] Inoculate the Burkholderia pyrrole JK-SH007 strain activated on the NA slant by streaking on both sides of the PDA plate, and then use a puncher with a diameter of 5mm to punch out the homogeneous Amount of bacterial block, spot in the middle of the plate. Cultivate at 28°C for 4 days to observe the presence or absence of the inhibition zone, and record its width (Table 1). The test results showed that JK-SH007 had strong antagonistic effects on the three pathogenic bacteria.

[0038] Table 1 Inhibition of JK-SH007 to three kinds of poplar canker pathogens

[0039]

Embodiment 2

[0040] Embodiment 2: Metabolites of Burkholderia pyrrole JK-SH007 are effective against 3 kinds of poplar canker pathogens (comprising Phomnopsis sp. (Phmnopsis sp.), Cytospora chrysosperma (Cytospora chrysosperma), Aesculus Plate inhibition of Thielavia aesculi (Fusicoccum aesculi).

[0041] Spread sterile cellophane on the PDA plate, spread and inoculate Burkholderia pyrrole JK-SH007, incubate at 30°C for 48 hours, remove the cellophane, inoculate pathogenic bacteria in the center of the plate respectively, and incubate at 28°C. With no birth control bacteria control, after the control mycelia covered the plate, the antagonistic activity was observed, and the bacteriostatic rate was counted (Table 2). The test results showed that the metabolites of JK-SH007 had strong antagonistic effects on the three pathogenic bacteria.

[0042] The metabolites of table 2 JK-SH007 inhibit three kinds of pathogenic bacteria

[0043]

Embodiment 3

[0044] Example 3: Growth promotion of Burkholderia pyrrole JK-SH007 on 150-day-old Populus americana seedlings.

[0045] Burkholderia pyrrole JK-SH007 was inoculated in NB medium for 48 hours of fermentation, and the seeds of Populus nigra were soaked in the 48-hour fermentation broth of the strain for 3 hours, rinsed with sterile water, and then sowed. The seeds were soaked in sterile water as a control. Seedling height 15cm Dingmiao, 4 plants per pot, measure seedling height and ground diameter after 150 days. Compared with the control, inoculation of JK-SH007 fermentation broth can significantly promote the growth of seedlings (Table 3). Compared with the control (CK), the treatment inoculated with JK-SH007 had significant differences in ground stem and seedling height, and the effect of promoting growth was obvious, see figure 1 .

[0046] Table 3 Effect of JK-SH007 on the growth of poplar seedlings

[0047]

[0048] Note: P<0.05

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Abstract

The invention discloses a burkholderia pyrrocinia JK-SH007 which is classified and named as burkholderia pyrrocinia and preserved in China center for type culture collection (CCTCC) with the preservation number of M 209028 and the preservation date of February 18th, 2009. The invention also discloses the application of the burkholderia pyrrocinia in controlling dothiorella gregaria and promoting poplar to grow. The invention further provides a bactericide for controlling dothiorella gregaria, which is obtained by inoculating the JK-SH007 in a culture medium to be fermented and cultured. The JK-SH007 is plant endophyte which has strong inhibiting effect for botryosphaeria dothidea of the poplar and antagonism for various pathogenic fungis, and has the advantages of simple culture condition, easy storage and commercial process as well as good developing and applying prospect.

Description

technical field [0001] The invention belongs to the field of plant protection, in particular to a Burkholderia pyrrole and its application in preventing and treating poplar canker. Background technique [0002] Poplar is an important fast-growing timber species with high economic value and ecological benefits. However, poplars are constantly threatened by pests and diseases during their growth. [0003] Poplar canker is a serious disease of poplar branches and stems. There are many kinds of pathogens and various symptoms, which seriously affect the growth of trees and even lead to the death of hosts. The usual names are canker, rot, skin rot, branch blight, etc. Most of the diseases are caused by fungi. The incidence of poplar canker is closely related to the growth potential of trees. When the host is affected by the environment and the growth potential is weakened, it is easy to occur. Therefore, young forests and dry and barren woodlands with insufficient water supply a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/02A01P3/00A01P1/00A01P21/00C12R1/01
Inventor 叶建仁任嘉红吴小芹王艳芳
Owner NANJING FORESTRY UNIV
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