50 results about "Botryosphaeria dothidea" patented technology

The invention relates to the technical field of microorganisms, and particularly discloses a saline-alkaline tolerant bacillus deserticola BW-13 with biocontrol and growth promoting functions and application thereof. The bacillus safensis BW-13 is preserved in the China Center for Type Culture Collection on January 13, 2025, the classification name of the bacillus safensis BW-13 is bacillus safeensis, and the preservation number of the bacillus safensis BW-13 is CCTCC NO: M 2025108. The strain BW-13 has relatively high salt tolerance, and still keeps good resistance under various stress conditions; meanwhile, the plant growth promoting agent has various plant growth promoting characteristics including nitrogen fixation, phosphorus solubilization and cellulase and protease secretion capacity, and pot experiment verifies that the plant growth promoting agent can improve the salt resistance of cucumber seedlings and promote plant growth; the strain has an antagonistic effect on a plurality of phytopathogens, and especially has a remarkable prevention and treatment effect on plant diseases such as apple ring spot, polygonatum kingianum anthracnose, pear tree rot and tomato gray mold caused by ascomycophyta pathogenic fungi.

The invention discloses dsRNA of a targeted BdSpt7 gene, a biocontrol agent and application of the dsRNA. The sequence of the dsRNA of the targeted BdSpt7 gene is as shown in SEQ ID No.2. The invention further discloses a preparation method of the biocontrol agent of the targeted BdSpt7 gene. The active ingredient of the biocontrol agent is the dsRNA of the target BdSpt7 gene. The invention also provides an application of the botryosphaeria dothidea BdSpt7 gene in prevention and treatment of apple botryosphaeria dothidea, and a nucleotide sequence of the botryosphaeria dothidea BdSpt7 gene is shown as SEQ ID No.1. The invention also provides an African trichoderma harzianum engineering strain for expressing dsRNA, the African trichoderma harzianum engineering strain is integrated with a dsRNA expression box for interfering gene expression of a target gene BdSpt7, the dsRNA expression box is shown as SEQ ID No.1, and the African trichoderma harzianum is an African trichoderma harzianum with the preservation number of CGMCC (China General Microbiological Culture Collection Center) No. 41709. The dsRNA targeting the BdSpt7 gene can significantly reduce the pathogenicity of the physalospora piricola through interference, and the African trichoderma harzianum engineering strain can colonize on the plant surface and continuously express the dsRNA so as to play a role in preventing and treating the physalospora piricola, and is green and environment-friendly.

The invention discloses trichoderma Guizhou GZMT12 and application thereof, and belongs to the technical field of microorganisms. The preservation number of the trichoderma Guizhou GZMT12 screened by the invention is CGMCC (China General Microbiological Culture Collection Center) NO. 42152. According to the present invention, the bacterial strain can provide inhibition effects on seven pathogenic bacteria such as Rhodococcus solani Kuhn, Botryosphaeria dothidea, Alternania alternania, Phomopsis litsea, Colletotrichum truncatum, Phytotrichum capsici, and Phytotrichum nitianae, and further has advantages of good prevention and treatment effect, wide antibacterial spectrum, wide application range, and the like.

The invention discloses a C2 spiro indolone compound as well as a preparation method and application thereof, simple and easily available N-indole connected o-alkynyl nitrobenzene compound is used as a raw material, and the C2 spiro indolone compound is obtained by heating reaction in a solvent under the catalysis of a cheap metal copper catalyst. The C2 spiro indolone compound prepared by the invention has a relatively good inhibition effect on phytopathogen, and can be used for preventing and treating botrytis cinerea, sclerotinia rot of colza and botryosphaeria dothidea; the preparation method does not need to use an expensive metal catalyst, does not need an additional reducing agent, and has the advantages of simple operation, high chemical selectivity, wide reaction substrate range and the like.

The invention belongs to the field of microorganisms, and discloses bacillus velezensis and application thereof in prevention and treatment of plant fungal diseases. The bacillus velezensis disclosed by the invention is bacillus velezensis YC46, is preserved in the China General Microbiological Culture Collection Center (CGMCC), and has the preservation number of CGMCC No.33975. The bacillus velezensis can grow by taking leftovers in an aquatic product processing process as a unique nutrient source, and has the advantages of high yield, low cost and the like. The bacillus subtilis has the antibacterial activity on plant pathogenic fungi such as fusarium oxysporum, botrytis cinerea, alternaria alternata, colletotrichum gloeosporioides and penicillium, thalli or fermentation products of the thalli can be used as active ingredients for preparing biocontrol microbial agents or bacteriostatic agents for preventing and treating plant fungal diseases, and the bacillus subtilis has good application value and ecological environmental protection value.

The invention belongs to the technical field of biology, and discloses Aspergillus tubingensis Pa6 and application of the Aspergillus tubingensis Pa6 in prevention and treatment of fruit tree diseases in order to improve the biological prevention and treatment effect of the fruit tree diseases, the strain number of the Aspergillus tubingensis is Pa6, and the registration number of the Aspergillus tubingensis in the General Microbiological Center of China Committee for Culture Collection of Microorganisms is CGMCC No.41994. The invention further discloses an application of the Aspergillus tubingensis Pa6 in the prevention and treatment of the fruit tree diseases. The Aspergillus tubingensis can prevent and / or treat strawberry gray mold, strawberry black spot or pear tree rot, or can be used for preparing products for preventing and / or treating strawberry gray mold, strawberry black spot or pear tree rot. The bacterial strain also has a relatively good antibacterial effect on fungi such as strawberry botrytis cinerea, strawberry black spot pathogen, apple ring spot pathogen, apple colletotrichum gloeosporioides, pear rot pathogen and the like.

The present application belongs to the technical field of plant disease prevention and treatment, and particularly relates to a biocontrol bacterium for preventing and treating Guaiacum officinale anthracnose, a fermentation liquor, a biocontrol bacterium agent and application. Bacillus velezensis A biocontrol bacterium is isolated and screened from a microbial resource on a healthy Guaiacum officinale leaf, and is classified and named as Bacillus velezensis , which is preserved in the China General Microbiological Culture Collection Center on March 28, 2025, and has a preservation number of CGMCC No.34038; the biocontrol bacterium provided in the present application has good antagonistic effects on Colletotrichum gloeosporioides causing Guaiacum officinale anthracnose, on Stemphylium botryosum causing Guaiacum officinale leaf spot disease, on Botryosphaeria dothidea, on Alternaria alternata and on Pestalotiopsis versicolor capable of causing plant diseases. The biocontrol bacterium, the fermentation liquor and the biocontrol bacterium agent provided in the present application have potential application prospects in preventing and treating Guaiacum officinale anthracnose.

The invention belongs to the field of strains, and particularly relates to a botryosphaeria dothidea attenuated strain ZMXX551-1 containing a penicillium-producing virus BdCV2 and application of the botryosphaeria dothidea attenuated strain. The attenuated strain ZMXX551-1 is preserved in the China General Microbiological Culture Collection Center on June 25, 2025, the preservation number is CGMCC NO.42040, the genome of the penicillium virus BdCV2 is composed of four dsRNAs, the penicillium virus BdCV2 has a remarkable effect in the aspect of weakening the pathogenicity of botryosphaeria dothidea, the attenuated strain ZMXX551-1 is used for treating peach seedlings, the length of scabs is inhibited by 8.63%, and the length of the scabs is inhibited by 8.63%. After 3 months, the scab of the treatment group begins to heal, and the scab of the control group is continuously increased, which indicates that the peach seedling inoculated with the strain ZMXX551-1 grows well; therefore, the strain ZMXX551-1 can inhibit expansion of peach gummosis scabs and improve plant growth, and has biological control potential.

The invention discloses an application of ethyl salicylate in improving apple fruit ring spot resistance, and relates to the technical field of plant disease resistance. According to the method, the apple fruits are soaked in the ethyl salicylate solution, so that the resistance of the apple fruits to the ring spot is improved, and the method is effective in various varieties. Experimental results show that the ethyl salicylate can inhibit the expansion of botryosphaeria dothidea plaque and improve the expression level of defense genes EDS1, PR1 and PR5. The method provided by the invention can overcome the barrier of preventing and treating the ring rot by using chemical agents and the like, greatly reduces the pollution of pesticides and the like to the ecological environment, is beneficial to reducing the loss caused by the occurrence of the ring rot of the apples in the mature period or the storage period, and provides an efficient and environment-friendly method for preventing and treating the ring rot and protecting the environment and human health.

The invention relates to a salt-tolerant bacillus strain EMM919, which is preserved in the China Center for Type Culture Collection on April 25, 2025, and the preservation number is CCTCC NO: M 2025879. The salt-tolerant bacillus strain EMM919 has the advantages that the salt-tolerant bacillus strain EMM919 is preserved in the China Center for Type Culture Collection on April 25, 2025; the nucleotide sequence of the strain comprises a 16S rRNA (ribosomal Ribonucleic Acid) gene sequence and a gyrB gene sequence. The bacterial strain can be used for preparing microbial pesticides and seed dressing agents for preventing and treating fusarium solani EMF893, neurospora EMF992, cylindrospora robusta EMF882, fusarium laminatum EMF998, alternaria alternata EMF993, botryosphaeria dothidea EMF899, solanum nigrum EMF990, phomopsis EMF898 and fusarium graminearum EMF897. The content of viable bacteria in the liquid pesticide is 2 * 10 < 9 >-5 * 10 < 9 > CFU / mL, and the content of viable bacteria in the solid pesticide is 2 * 10 < 9 >-8 * 10 < 9 > CFU / g. In the liquid seed-dressing agent, the content of viable bacteria is 2 * 10 < 9 >-5 * 10 < 9 > CFU / mL, and in the solid seed-dressing agent, the content of viable bacteria is 2 * 10 < 9 >-8 * 10 < 9 > CFU / g.

The present application belongs to the technical field of fertilizer, and specifically discloses a foliar fertilizer for improving the disease resistance effect of Vietnamese ginseng, which is composed of 0.1-0.5% potassium dihydrogen phosphate, 0.1-0.2% boric acid, 0.3-0.7% urea, 0.005-0.02% chitosan, 0.0001% salicylic acid and 0.05% Tween-20, and the rest is water, according to the weight percentage. The foliar fertilizer prepared by the present application has a remarkable disease control effect on Vietnamese ginseng, and has a positive control effect on the infection of Fusarium oxysporum, Alternaria panax and Botryosphaeria dothidea. After using the foliar fertilizer of the present application, the growth of the pathogen is significantly inhibited from the phenotype, and the disease onset time of Vietnamese ginseng is significantly later than that of the control. The resistance gene expression of Vietnamese ginseng is significantly improved through testing.

The invention discloses application of streptomyces SC-3 volatile organic compounds in inhibition of botryosphaeria dothidea, which comprises the following steps: preparing a streptomyces SC-3 wheat grain culture, inoculating SC-3 bacterial suspension to a sterilized wheat grain culture medium, culturing for 5-14 days at the temperature of 25-30 DEG C, and regularly shaking up during the culture period; the wheat grain culture is in gas-phase contact with B. dothidea by adopting a double-dish appointment method, an upper dish is a PDA culture medium containing B. dothidea fungus cakes, the wheat grain culture is placed in a lower dish, and the wheat grain culture is cultured for 2-5 days at the temperature of 25-30 DEG C. According to the invention, botryosphaeria dothidea (B. dothidea) is inhibited through volatile organic compounds (VOCs) of streptomyces SC-3, and a remarkable antibacterial effect is shown.

The invention relates to the technical field of pesticides, in particular to an acrylate compound containing a biphenyl structure as well as a preparation method and application of the acrylate compound. The acrylate compound containing the biphenyl structure is a compound as shown in a formula I, or a stereoisomer, a tautomer, an isotope derivative and a pesticide acceptable salt thereof. The acrylate compound containing the biphenyl structure has excellent inhibitory activity on plant pathogenic fungi, especially basidiomycota, ascomycomycota and deuteromycota pathogenic fungi, and can be used for preventing and treating plant diseases generated by the pathogenic fungi in the fields of agriculture or forestry and the like; the bactericidal composition has very good effect and selectivity on prevention and treatment of wheat powdery mildew, soybean rust, corn rust, peach brown rot, wheat sharp eyespot, rice sheath blight disease or apple ring rot, and has outstanding crop safety on crops.

The application belongs to the technical field of microorganisms, and particularly relates to a bacillus altitudinis BW65 strain against pear white line feather disease and application of the bacillus altitudinis BW65 strain in disease resistance, yield increase and quality improvement. Bacillus altitudinis The strain is isolated from the rhizosphere soil of a peach tree and is classified and named as the BW65 strain. The strain has the abilities of IAA production, nitrogen fixation, organic phosphorus and inorganic phosphorus decomposition, can significantly promote the growth of pear, tomato and other plants, improve the root morphology, increase the biomass and fruit quality, and has antagonistic effects on multiple pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and botryosphaeria dothidea. Application of the bacterial agent can improve the yield and quality of crops, reduce the use of chemical fertilizers and pesticides, and is suitable for the preparation of biological organic fertilizer and green agricultural production.

The invention provides a heterocyclic ring-containing nicotinamide compound, a synthesis method and application of the heterocyclic ring-containing nicotinamide compound in prevention and treatment of plant fungal diseases, and relates to the technical field of pesticides. The heterocyclic ring-containing nicotinamide compound is synthesized by using easily available raw materials and a simple, practical and low-cost synthesis method. The compound has a synergistic effect on rhizoctonia solani, magnaporthe oryzae, mango anthracnose pathogen, rubber anthracnose pathogen, litchi anthracnose pathogen, banana anthracnose pathogen, coffee anthracnose pathogen, fusarium graminearum, wheat powdery mildew pathogen, valsa mali, botrytis cinerea, sclerotinia sclerotiorum, sigatoka musae, banana scab pathogen, apple ring spot pathogen, botrytis cinerea, botrytis cinerea, botrytis cinerea, botrytis cinerea and botrytis cinerea. The compound has an excellent inhibiting effect on plant fungal diseases caused by fungi such as tobacco alternaria alternata, cotton rhizoctonia solani, exserohilum turcicum and hevea brasiliensis, is novel in structure, and has huge potential for creation of novel pesticides.

The invention discloses application of 2-methylbutyric acid in prevention and treatment of postharvest diseases of mangifera indica. The diseases comprise mangifera indica anthracnose and mangifera indica stem-end rot. Wherein the pathogenic bacteria of the mango anthracnose comprise Asian colletotrichum gloeosporioides; the pathogenic bacteria of the mango stem-end rot comprise botryosphaeria dothidea and / or physosphaeria sojae. It is found for the first time that 2-methylbutyric acid has significant inhibitory activity on main pathogens after mango picking, and especially shows an efficient fumigation bacteriostatic effect on colletotrichum asiaticum causing mango anthracnose, botryosphaeria dothidea causing mango stem-end rot, and soybean mesotheca dothidea causing mango stem-end rot. 2-methylbutyric acid is mild in smell, so that the adverse effect on the original flavor of mangifera indica can be reduced to a greater extent.

The application discloses a biocontrol pantoea agglomerans KBA33 for inhibiting botryosphaeria dothidea and application thereof. The strain KBA33 disclosed by the application can effectively inhibit botryosphaeria dothidea at 16 DEG C and 28 DEG C, and can inhibit botryosphaeria dothidea through cell-cell contact.

The bacillus velezensis strain EMM914 is preserved in the China Center for Type Culture Collection on May 19, 2025, and the preservation number of the bacillus velezensis strain EMM914 is CCTCC (China Center for Type Culture Collection) NO: M 20251091. The nucleotide sequence comprises a 16S rRNA (ribosomal Ribonucleic Acid) sequence and a gyrB sequence, which are as shown in SEQ ID No. 1 and SEQ ID No. 2. The strain EMM914 can be used for preparing medicines for preventing and treating fusarium moniliforme EMF994, fusarium oxysporum EMF889, colletotrichum gloeosporioides EMF991, fusarium graminearum EMF897, fusarium laminatum EMF998, black beancurd skin fungus EMF990, fusarium solani EMF893, fusarium oxysporum EMF996, botryosphaeria dothidea EMF899, fusarium solani EMF895, fusarium trifida EMF997, pear black beancurd skin fungus EMFB1, fusarium oxysporum EMF894, fusarium trifida EMF896, alternaria alternata EMF993, fusarium solani EMF1001, fusarium According to the application of the compound in microbial pesticides for any fungal disease in botrytis cinerea EMFHM, the inhibition rate eta of 19 pathogenic bacteria is 65% or above.

The present application provides a strain of Trichoderma asperellum TAZ19, a biocontrol agent and application thereof, wherein the Trichoderma asperellum TAZ19 is preserved in China Center for Type Culture Collection on October 25, 2024, and the preservation number is CCTCC M 20242341. The active ingredient of the biocontrol agent comprises the above-mentioned Trichoderma asperellum TAZ19 strain, and the Trichoderma asperellum TAZ19 strain is one or more of conidium, mycelium, fermentation filtrate or active extract thereof. The Trichoderma asperellum TAZ19 has excellent antagonistic effect on multiple plant disease pathogens such as Diaporthe phaseolorum, multiple Fusarium oxysporum, Valsa mali, and Botryosphaeria dothidea, has broad-spectrum antibacterial property, fast growth, high antibacterial activity, strong parasitism, and good salt and alkali tolerance, and has a growth-promoting effect on plants. The Trichoderma asperellum TAZ19 can be widely applied to the prevention and treatment of multiple plant diseases such as pine branch and stem rot, wheat stem base rot, tomato wilt, and has a wide application prospect.

The application discloses a method for cultivating Morinda citrifolia over summer in grape forest, and aims at the problems of low yield and difficult mushrooming of Morinda citrifolia caused by high temperature in summer in Qiannan, and utilizes the grape forest intercropping Morinda citrifolia with the shade and rain-sheltering canopy. The method is characterized in that the mycelium full-bagging stick is cultivated over summer through the way of arranging the stick in the field, the environment is timely regulated according to different growth stages, and the nutrient solution and microbial liquid inoculum are innovatively applied in each stage. The method has the following advantages: the natural shading and temperature adjustment of grape branches and leaves are utilized, the cost of 0.8 million yuan per mu of the shed is saved, the biological conversion rate is increased by 3%, the fresh mushroom yield in summer reaches 600 g per stick, the mode of 'grape-Morinda citrifolia-waste mushroom dregs-grape' is constructed, the grape branches are utilized to produce the stick, the waste mushroom dregs are utilized to improve the soil, the mode of 'forest for fungus and fungus for forest' is realized, and the occurrence rate of diseases and insect pests is reduced by more than 8%. The method not only improves the land utilization rate and unit output, but also is easy to popularize, and has important significance for promoting the development of the forest economy.

The invention discloses a strain of salt-tolerant bacillus (bacillus halotolans) with a biocontrol effect as well as a microbial agent and application of the salt-tolerant bacillus halotolans. The salt-tolerant bacillus is named as bacillus halotolans 1-64 and is preserved in the China Center for Type Culture Collection, and the preservation number of the salt-tolerant bacillus is CCTCC (China Center for Type Culture Collection) NO: M20252455. Experiments prove that the strain has nitrogen fixation capacity, can generate non-volatile and volatile antibacterial metabolites with thermal stability, and has a remarkable inhibition effect on various important plant pathogenic fungi such as phytophthora nicotianae, alternaria alternata, fusarium oxysporum, penicillium expansum, botryosphaeria dothidea, colletotrichum gloeosporioides and rhizoctonia solani. The invention also optimizes the fermentation medium formula and culture conditions of the strain, and obviously improves the biomass of the strain. The strain 1-64 has a wide application prospect in the aspect of being developed into efficient, stable and environment-friendly biopesticides.

The invention provides an ethyl ferulate liposome as well as a preparation method and application thereof in resisting soft rot of kiwi fruits. According to the application disclosed by the invention, the differences of three compounds of ferulic acid, methyl ferulate and ethyl ferulate on in-vitro growth inhibition of kiwi fruit soft rot pathogenic bacteria-botryosphaeria dothidea mycelia are screened and compared, and the condition that the ethyl ferulate has a better inhibition effect on the botryosphaeria dothidea is determined; liquid grease and solid grease are used as wall materials, ethyl ferulate is used as a core material, and the nano ethyl ferulate liposome is prepared by adopting a melt emulsification ultrasonic method. The prepared nano ethyl ferulate liposome is uniform in particle size, and compared with non-embedded ethyl ferulate, the bacteriostatic activity and stability of the ethyl ferulate liposome are remarkably improved, and the nano ethyl ferulate liposome has a better application prospect in resistance to soft rot of kiwi fruits.

The application provides application of CYP51 protein of Botryosphaeria dothidea in anti-azoles, and belongs to the field of agricultural biotechnology. The application provides application of a reagent for detecting mutation of CYP51 gene sequence or CYP51 protein in Botryosphaeria dothidea in evaluation of azole resistance of Botryosphaeria dothidea. Whether mutation occurs to the 406~408bp position of the CYP51 gene or the 136th tyrosine of the CYP51 protein can effectively evaluate the azole resistance of Botryosphaeria dothidea. The application provides a model of Botryosphaeria dothidea with high azole resistance and a construction method thereof. The model can be used for research and preparation of drugs for prevention and treatment of Botryosphaeria dothidea. The application provides an identification method for azole-resistant Botryosphaeria dothidea and application of the identification method in development monitoring of azole resistance of Botryosphaeria dothidea. The application has important significance for agricultural disease prevention and control and rational use of pesticides, and helps to improve the sustainability and economic benefits of agricultural production.

This invention belongs to the field of biological control of plant diseases, specifically relating to a virulent strain of *Botrytis cinerea* SdBd-6 and its applications. The virulent strain of *Botrytis cinerea* SdBd-6 is classified and named... Botryosphaeria dothidea The attenuated strain SdBd-6 of Staphylococcus aureus was deposited at the Guangdong Provincial Center for Microbial Culture Collection on January 14, 2026, with accession number GDMCC: 67656. Walsa money The growth of the fungus has a certain inhibitory function. At the same time, after 10 generations of continuous inoculation, the virus contained in the attenuated strain SdBd-6 of *Botrytis cinerea* was not lost, and its growth characteristics and pathogenicity remained attenuated strain characteristics. This provides materials and lays the foundation for the green control of plant fungal diseases caused by the pathogenic fungus *Botrytis cinerea*.

The application discloses a kind of active substances for crop protection and its composition and preparation method and application, the substance general formula as follows: Wherein: R1 It is hydrogen atom, halogen, cyano, methyl, methoxy, nitro, trifluoromethyl or the disubstituted of arbitrary combination of above halogen;R2 It is hydrogen atom, halogen or nitro substitution.This application derivative shows better inhibitory effect to rice bacterial leaf spot, rice bacterial leaf blight, kiwi bacterial canker, citrus canker.The inhibitory activity of compound II-18 to kiwi bacterial canker bacteria is best.Plant pathogenic fungi (sclerotinia sclerotiorum, phomopsis viticola, botryosphaeria dothidea and fusarium graminearum) inhibitory activity test shows that compound II-10 shows better inhibitory activity to fusarium graminearum.In addition, part of the derivatives of the application has significant contact toxicity to bactrocera dorsalis (Hendel).The composition of compound II-5 and methyl eugenol shows synergistic trapping effect in the process of preventing and treating the main pest of kiwi, bactrocera dorsalis (Hendel).

The invention relates to the technical field of microorganisms, in particular to a bacillus altitudinis BW65 capable of resisting pear white rot and application of the bacillus altitudinis BW65 in disease resistance, yield increase and quality improvement, and the strain is separated from rhizosphere soil of peach trees and is classified and named as Bacillus altitudinis BW65. The strain has the capabilities of producing IAA, fixing nitrogen and decomposing organic phosphorus and inorganic phosphorus, can remarkably promote the growth of plants such as pears and tomatoes, improve the root morphology and improve the biomass and the fruit quality, and has an antagonistic effect on various pathogenic bacteria such as rice blast, alternaria alternata, fusarium graminearum and hard funguses. By applying the microbial agent, the crop yield and quality can be improved, the use of chemical fertilizers and pesticides is reduced, and the microbial agent is suitable for bio-organic fertilizer preparation and green agricultural production.

The invention discloses endo-xylanase BdGH11 derived from botryosphaeria dothidea, a coding gene sequence of the endo-xylanase BdGH11, a preparation method of the endo-xylanase BdGH11, and an application of the endo-xylanase BdGH11. The invention also provides a method for preparing the novel xylanase, namely a technical method of genetic engineering is utilized to clone the gene of the novel xylanase onto an escherichia coli expression vector to obtain an escherichia coli recombinant strain capable of heterologously expressing the novel xylanase, and the endoxylanase BdGH11 prepared by heterologously expressing the escherichia coli recombinant strain is used for preparing the endo-xylanase BdGH11. The xylo-oligosaccharide can be efficiently produced, the monosaccharide content is low, and the endonuclease can effectively stimulate the immune response of plants. The xylanase provided by the invention has higher enzyme activity under the condition of 30-45 DEG C, has degradation preference on xylan, is high in storage stability, and can be widely applied to the fields of brewing, feed industry and the like.

The invention discloses application of a carbazole ring-containing natural product as an agricultural bactericide in plant diseases. The carbazole ring natural product has a good inhibition effect on plant bacterial diseases such as rice bacterial blight, citrus canker, tobacco bacterial wilt, kiwifruit canker or rice bacterial streak, and the ellipticine can be used as an innovative medicament for resisting rice bacterial blight, and the medicament shows a remarkable sterilization effect; aiming at plant fungal diseases such as cucumber gray mold, pepper fusarium wilt, sclerotinia rot of colza, wheat scab, potato late blight, blueberry root rot, botryosphaeria dothidea, pitaya anthracnose, rice sheath blight disease, fusarium SP or colletotrichum graminearum and the like, the invention provides a new application of a carbazole ring-containing natural product which can be used for preparing a plant fungicide. The composition can be used for preventing and treating plant diseases in the agricultural field.

The invention provides a kiwi fruit fresh-keeping biocontrol bacterium and application thereof, the bacterial strain is classified and named as Bacillus amyloliquefaciens BacXF14, the preservation number of the bacterial strain is CGMCC (China General Microbiological Culture Collection Center) NO: 35691, and the preservation date is August 20, 2025. The bacterial strain has bacteriostatic activity on botryosphaeria dothidea, alternaria alternata, phomopsis, phomopsis vitis, botrytis cinerea and pestalotiopsis. The strain can effectively inhibit the growth of various pathogenic bacteria after harvesting of kiwi fruits, and the occurrence of soft rot caused by botryosphaeria dothidea infection is remarkably reduced. The composition of microbial community structures on the surfaces of the kiwi fruits can be regulated and controlled, the relative abundance of spoilage pathogenic bacteria is reduced, and the relative abundance of beneficial microorganisms is increased, so that the quality of the kiwi fruits is improved, and the preservation period of the fruits is prolonged. In conclusion, the strain has wide application potential and value in the postharvest preservation process of the kiwi fruits.

The invention discloses an isophorone derivative and application thereof, the isophorone derivative has excellent bacteriostatic action on rhizoctonia solani, botrytis cinerea, sclerotinia sclerotiorum, fusarium graminearum, valsa mali and physalospora piricola, and can be used for preventing and treating various plant fungal diseases.