The invention discloses a primer group for rapidly and visually identifying
Bactrocera mangifera and application of the primer group. The invention belongs to the technical field of
biology, and particularly relates to a primer group for rapidly and visually identifying
Bactrocera mangifera and application of the primer group. The invention relates to a composition for identification or auxiliary identification of
Bactrocera mangifera, the composition is a composition A or a
composition B, the composition A is composed of an upstream primer named as Ccos-1F and a downstream primer named as Ccos-1R, and the
composition B is composed of an upstream primer named as Ccos-1F and a downstream primer named as Ccos-1R; the
composition B is composed of an upstream primer with the name of Ccos-1F, a downstream primer with the name of B-Ccos-1R, and a fluorescent probe sequence Ccos-Probe1 specific to the to-be-detected
bactrocera indica. The MIRA-LFD primer disclosed by the invention is more flexible in design, high in sensitivity (capable of detecting trace
DNA) and strong in specificity, is particularly suitable for on-site
rapid detection requirements of port
quarantine,
field monitoring and the like, and provides an efficient
technical support for prevention and control of the Bactrocera mangifera.