The invention relates to the technical field of
tissue culture in
plant biotechnology, in particular to a rapid propagation culture medium and method for in-vitro regeneration of stem segments and petioles of Shuidong
wikstroemia macrocephala. According to the specific technical scheme, a rapid propagation culture medium is based on 1 / 2 MS, and 0.5 mg / L of 6-BA, 0.2 mg / L of NAA and other components are added. According to the invention, the defect that leaves and other plants (such as
rubus corchorifolius, olive kernel and neoparthenocissus nepalensis) cannot be regenerated by using the same formula is overcome, it is found and proved for the first time that the
hormone composition only has a specific efficient
induction effect on stems and petioles of Shuidong
wikstroemia macrocephala, and the Shuidong
wikstroemia macrocephala can be guided to rapidly differentiate calluses, so that the growth of Shuidong wikstroemia macrocephala can be promoted. And cluster buds can be rapidly differentiated from the calluses. According to the method, the propagation path is direct and efficient, and a core technology is provided for resource preservation and
seedling raising of the Shuidong wikstroemia macrantha.