8 results about "Gracilaria" patented technology

The invention belongs to the technical field of algal polysaccharide extraction, and discloses a modification extraction method of algal asparagus polysaccharide and a method for preparing a lutein coating by using the algal asparagus polysaccharide. The modified extraction method comprises the following steps: extracting gracilaria lemaneiformis powder with a weak base solution, enabling lactide and polysaccharide to be subjected to grafting reaction through a redox initiation system under the assistance of ultrasonic waves, and then separating out a first polysaccharide component with high emulsibility and a second polysaccharide component with high gel property through graded alcohol precipitation. The two obtained functional components are used for coating xanthophyll, specifically, the first polysaccharide component emulsifies a xanthophyll oil phase to form a pre-emulsion, the second polysaccharide component is compounded with gelatin, a composite coacervate is formed on an oil drop interface by adjusting the pH value, and finally drying and curing are conducted. According to the method, two modified polysaccharides with definite functions can be synchronously obtained, the prepared xanthophyll coating is high in encapsulation efficiency, good in storage and processing stability and excellent in water dispersibility, and the problems that xanthophyll is unstable and difficult to apply are effectively solved.

This invention provides an automatic seedling clamping device and method for Gracilaria rapa, including a seedling separating mechanism, a conveying mechanism, a seedling clamping mechanism, a rope releasing mechanism, a rope clamping mechanism, a transmission mechanism, and a rope cutting mechanism. The seedling separating mechanism includes a feeding funnel and a sorting and cutting assembly. First, Gracilaria rapa seedlings are placed into the feeding funnel. The sorting and cutting assembly sorts and cuts the Gracilaria rapa into seedling clusters. The conveying mechanism conveys the seedling clusters to the seedling clamping worktable. The rope releasing mechanism includes two rope winding frames with a winding drum on each frame. The seedling clamping mechanism includes a seedling clamping worktable and a push plate assembly. The winding drum rotates relative to the rope winding frames to release the ropes. The released ends of both ropes are clamped by the first clamp of the rope clamping mechanism. The first clamp moves to the left while rotating back and forth to wind the two ropes together. The push plate assembly pushes the Gracilaria rapa seedling clusters fed by the conveying mechanism to the point where the ropes are joined to complete the seedling clamping. The rope cutting mechanism cuts the clamped ropes. The seedling clamping efficiency is high and the operation is easy.

A preparation method of a seaweed starter includes: wetting cooked soybean meal with water, adding wheat meal and stirring same, adding seaweed meal to obtain a fermentation raw material and sterilizing same; adding a starter-making strain spore suspension into the sterilized fermentation raw material, performing constant-temperature culture, and performing ventilation and starter making for 48-96 hours to obtain a seaweed starter. The starter-making strains are aspergillus oryzae and aspergillus niger, and wherein in terms of a mass ratio, a strain ratio of aspergillus oryzae to aspergillus niger is 1:1-1:5, an inoculum amount of the starter-making strain spore suspension is 3-5% of the fermentation raw material, and the seaweed meal is Gracilaria meal; and wherein in terms of a mass ratio, an addition ratio of the cooked soybean meal to the wheat meal to the Gracilaria meal is 5:2:1-3.

The invention belongs to the field of cell engineering seedling culture of algae, and particularly relates to a cell engineering seedling culture method of gracilaria lemaneiformis. The gracilaria lemaneiformis frond is constructed into a miniaturized form, and high-density intensive culture is carried out by utilizing a photobioreactor. According to the method, the microminiaturized asparagus frond obtained by a cell engineering means can be subjected to vegetative propagation, efficient propagation is carried out by utilizing the algae photobioreactor, and the microminiaturized asparagus frond is further cultured into seedlings for artificial cultivation.

This invention relates to a composition for preventing colitis-associated colorectal cancer and controlling weight, comprising seahorse, laver, kelp, spirulina, Ulva procumbens, and Gracilaria in a specific ratio. This formula is based on traditional Chinese medicine theories such as "five colors entering the five internal organs," "five flavors entering the meridians," and "salt can soften hardness," while also considering the combined use of warming and cooling properties, and the integration of clearing and tonifying. Through the combination of various marine algae and seahorse, it achieves synergistic effects on multiple targets, including immune regulation, anti-inflammation, intestinal barrier repair, and weight control. The preparation method is simple and low-cost, suitable for large-scale production in food and dietary supplements. Animal experiments show that this composition can significantly slow weight gain in normal mice. In an AOM / DSS-induced colitis-associated colorectal cancer model, it can reduce the disease activity index, decrease the number of colon tumors, alleviate abnormal organ enlargement and intestinal pathological damage, downregulate inflammatory factors such as iNOS and Cox-2, and upregulate intestinal barrier-related factors such as Cdh1, demonstrating a good preventive intervention effect.

This invention discloses a red tide control method based on year-round cultivation of Gracilaria fusiforme, belonging to the field of red tide control. The method includes: selecting sea areas requiring prevention or restriction of red tide outbreaks, or adjacent sea areas, as cultivation zones; using heat-resistant and rapidly growing Gracilaria fusiforme seedlings; and implementing a year-round multi-crop rotation cultivation system centered on seedling introduction from April to September to ensure that Gracilaria fusiforme is in its vigorous growth period during the peak red tide season in summer, continuously absorbing nutrients such as nitrogen and phosphorus from the water, thus achieving dynamic regulation of nutrient levels in the sea area and suppressing red tide occurrences at the source. This invention, through year-round multi-crop rotation cultivation, precisely covers the peak red tide season in summer, breaking through the bottleneck of the misalignment between traditional seaweed cultivation and red tide control. Simultaneously, as an economically valuable seaweed, Gracilaria fusiforme can create significant socio-economic benefits during ecological restoration, forming a long-term mechanism that synergizes environmental, ecological, and economic benefits, providing an efficient, environmentally friendly, and economically valuable innovative solution for red tide control.

The invention belongs to the technical field of marine organisms, and relates to an LAMP (loop-mediated isothermal amplification) primer probe combination and a kit for identifying a gracilaria lemaneiformis culture strain 981 and application of the LAMP primer probe combination and the kit, the nucleotide sequence of FIP of an inner primer is as shown in SEQ ID NO.1, the nucleotide sequence of BIP of the inner primer is as shown in SEQ ID NO.2, the nucleotide sequence of F3 of an outer primer is as shown in SEQ ID NO.3, and the nucleotide sequence of B3 of the outer primer is as shown in SEQ ID NO.4. The method is applied to large red alga strain identification for the first time, a four-primer LAMP system is developed on the basis that two sites are inserted into one InDel specific to the 981 strain, and during LAMP amplification, FIP primer modification biotin and BIP primer labeling FAM are combined with a streptavidin detection line of an LFD test strip, so that naked eye interpretation without electrophoresis is realized, the identification time is greatly shortened, and on-site rapid detection can be performed.

The invention provides a method for expanding propagation and seed production of gracilaria lemaneiformis seedlings in a high-temperature period in a southern sea area, and particularly belongs to the technical field of expanding propagation and seed production of gracilaria lemaneiformis seedlings. The method comprises the following steps: breeding gracilaria lemaneiformis fries by adopting a long rope type breeding method; the water layer depth of cultivation is adjusted through sinking and floating of the seedling ropes, the water layer depth of cultivation comprises a first water depth and a second water depth, the first water depth is 0.3-0.6 m, and the second water depth is 1.5-2.0 m; the flow velocity of the reciprocating flow in the sea area for cultivation is 0.5-1.2 m / s; the illumination intensity of the second water depth in the noon period is 300-800 lux. By means of the method, propagation and seed production of gracilaria lemaneiformis seedlings can be achieved in high-temperature seasons, and annual seed conservation and seed production of gracilaria lemaneiformis in southern sea areas are achieved.