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Preparation method for extracting gracilaria agar by replacing alkaline process with enzymatic method

An alkaline extraction and Gracilaria technology, which is applied in the field of preparation of enzymatic substitution for alkaline extraction of Gracilaria agar, can solve problems such as environmental hazards, reduce production costs, be beneficial to environmental protection, reduce consumption and sewage discharge Effect

Active Publication Date: 2016-06-01
JIMEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the use of strong acid, strong alkali and bleach will undoubtedly bring certain harm to the environment. At the same time, with the rise of the prices of production raw materials such as acid, alkali and bleach, and the gradual emergence of problems such as energy shortages, the use of alkaline extraction of agar The high cost problem also puts the production enterprises in a dilemma

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A preparation method for extracting Gracilaria agar by enzymatic method instead of alkali method, comprising the following steps:

[0022] Enzyme treatment: Weigh 50g Gracilaria, crush it, add water to soak, add water 20 times the mass of Gracilaria, adjust the temperature to 50°C, then add neutral cellulase, adjust the cellulase activity in the system to 10U / mL; Then add neutral protease to adjust the activity of protease in the system to 4U / mL; finally add lipase to adjust the activity of lipase in the system to 3U / mL; the time for enzymatic treatment of Gracilaria is 6h, after the treatment, centrifuge to obtain Gracilaria residue .

[0023] Glue cooking and decolorization: the amount of water added is 12 times the mass of Gracilaria, boiled under normal pressure until Gracilaria is completely melted, and then macroporous adsorption resin is added. The mass fraction of the added resin is 2%, and the content of EDTA is 0.012%. The adsorption time is 3 hours, and fina...

Embodiment 2

[0028] A preparation method for extracting Gracilaria agar by enzymatic method instead of alkali method, comprising the following steps:

[0029] Enzyme treatment: Weigh 2.5kg Gracilaria, crush it, add water to soak, add water 15 times the mass of Gracilaria, adjust the temperature to 48°C, then add neutral cellulase, adjust the cellulase activity in the system to 12U / mL Then add neutral protease, the protease activity in the regulation system is 5U / mL; finally add lipase, the lipase activity in the regulation system is 4U / mL; the time of enzymatic treatment of Gracilaria is 6h, after finishing the centrifugation to obtain Gracilaria scum.

[0030] Glue boiling and decolorization: the amount of water added is 12 times the mass of Gracilaria, boiled under normal pressure until Gracilaria is completely melted, and then macroporous adsorption resin is added. The mass fraction of the added resin is 3%, and the content of EDTA is 0.012%. The adsorption time is 3.5h, and finally fi...

Embodiment 3

[0035] A preparation method for extracting Gracilaria agar by enzymatic method instead of alkali method, comprising the following steps:

[0036] Enzyme treatment: Weigh 25kg of Gracilaria, crush it, soak it in water, add water 30 times the mass of Gracilaria, adjust the temperature to 50°C, then add neutral cellulase, and adjust the cellulase activity in the system to 15U / mL; Then add neutral protease to adjust the activity of protease in the system to 6U / mL; finally add lipase to adjust the activity of lipase in the system to 4.5U / mL; the time for enzymatic treatment of Gracilaria is 8h, and the enzymatic solution is filtered off after treatment .

[0037] Glue cooking and decolorization: the amount of water added is 18 times the mass of Gracilaria, boiled under normal pressure until Gracilaria is completely melted, and then macroporous adsorption resin is added. The mass fraction of the added resin is 5%, and the content of EDTA is 0.012%. The adsorption time is 4 hours, a...

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Abstract

The invention discloses a preparation method for extracting gracilaria agar by replacing an alkaline process with an enzymatic method. The preparation method for extracting the gracilaria agar by replacing the alkaline process with the enzymatic method comprises the following steps: firstly, carrying out enzyme treatment, namely smashing gracilaria, adding water, soaking, adjusting the temperature to be 45-50 DEG C, then adding neutral cellulase, then adding neutral protease, and finally adding lipase; secondly, boiling glue and decolorizing, namely adding water and boiling glue, boiling at ordinary pressure until gracilaria is completely melted, adding macroporous adsorption resin and EDTA, adsorbing for 3-4 hours, and filtering; thirdly, carrying out enzyme treatment, namely cooling to-be-filtered glue solution to 45-50 DEG C, and adding sulfatase for removing sulfuric acid groups of agar, wherein the enzyme treatment is carried out for 3.5-5 hours; and fourthly, obtaining the finished product, namely after the enzyme treatment is finished, quickly cooling, pushing into strips, subpackaging, carrying out oil pressure dehydration, drying, smashing, and collecting screened powder. The preparation method disclosed by the invention adopts a biological whole enzymatic method for replacing application of acid, alkaline and a bleaching agent in an agar extracting process, has the characteristics of environmental friendliness and no pollution and has important significance on sustainable development of an agar production enterprise.

Description

technical field [0001] The invention relates to the technical field of food processing, in particular to a preparation method for extracting Gracilaria agar by enzymatic method instead of alkaline method. Background technique [0002] Gracilaria is rich in a large amount of colloid, which is an important raw material for making agar. Gracilaria is also rich in a variety of other components, and its composition and composition ratio vary greatly with its species, growth sea area, collection season, and farming methods; polysaccharides and crude fiber are the most important components of Gracilaria , accounting for 63.10% to 75.97% of Gracilaria algae; Gracilaria is also rich in various natural pigment proteins such as phycoerythrin and phycocyanin, and the protein content accounts for 13.82% to 26.98% of the algae, with an average of 20.52%; fat is in Between 0.84% ​​and 2.50%; in addition, because Gracilaria can absorb mineral elements in seawater and enrich them in the alg...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/12
CPCC08B37/0039
Inventor 肖安风肖琼倪辉吴昌正姜泽东蔡慧农朱艳冰杨秋明杜希萍许彩云
Owner JIMEI UNIV
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