Cross breeding method among venussear mottles of different geographical population

A hybridization method, the technology of variegated abalone, applied in the field of genetic improvement of variegated abalone, to achieve the effect of promoting synchronous release of gametes and strong operability

Inactive Publication Date: 2006-02-22
XIAMEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to provide a hybridization method between different geographic populations of variegated abalones in view of the shortcomings in the existing production methods of abalone seedlings and the long-term failure to solve the problem of using variegated abalones between different geographical populations

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] In Japan, the abalone with a size of 2.5-3 cm is gradually lowered from the normal water temperature to the transportation temperature of 18 °C at a cooling rate of 2 °C per hour. After the transportation arrives at the destination, it is placed in seawater at 18°C, and then gradually rises to normal water temperature at a rate of 1°C per hour. The survival rate during transportation reaches 90%. According to the living habits of Japanese variegated abalone, the synchronous ripening culture of parent abalone was carried out, and the bait was fed according to the ratio of 100 Gracilaria, 40 Ulva, 30 Wakame, and 8 Spirulina. Select the Japanese abalone with full gonads, inject 1ml of each head or abdomen with a concentration of 10 -3 mol / L of 5-hydroxytryptamine, ejaculate after 2 to 3 hours. The sperm released from Japanese variegated abalone (male) and the eggs released from Taiwan variegated abalone (female) were mixed according to a certain ratio, and the hybrid offs...

Embodiment 2

[0013] Similar to Example 1, the difference is that the abalone with a size of 2 to 2.5 cm is gradually lowered from the normal water temperature to the transportation temperature of 17° C. at a cooling rate of 3° C. per hour. After the transportation arrives at the destination, put it into seawater at 17°C, and then gradually raise the temperature to normal water temperature at a rate of 0.2°C per hour. When carrying out synchronous maturation-promoting cultivation of parent-aubalone, mix bait and feed according to the ratio of Gracilaria 100, Ulva 30, Undaria pinnatifida 50, and Spirulina 15. Inject 1ml into the head or abdomen of each abalone with a concentration of 1.2×10 -3 mol / L of 5-hydroxytryptamine, ejaculate after 2 to 3 hours.

Embodiment 3

[0015] Similar to Example 1, the difference is that the 3.5-4cm abalone is gradually lowered from the normal water temperature to the transport temperature of 16°C at a cooling rate of 1°C per hour. After the transportation arrives at the destination, put it into seawater at 16°C, and then gradually raise the temperature to normal water temperature at a rate of 0.5°C per hour. When carrying out synchronous maturation-promoting cultivation of parent abalones, feed them with mixed bait according to the proportion of Gracilaria 100, Ulva 60, Undaria pinnatifida 20, and Spirulina 1. Inject 0.8ml into the head or abdomen of each abalone with a concentration of 0.8×10 -3 mol / L of 5-hydroxytryptamine, ejaculate after 2 to 3 hours.

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Abstract

A crossbreed method for different geographical species parti-colour abalone, which relates to a genetic improved method for parti-colour abalone, especially relates to a crossbreed method using different geographical species parti-colour abalone which comprises, putting the abalone in 16-18Deg C. sea water and elevating temperature to make the parent abalone accelerate. The bait comprises gracilaria 100, Ulva lactuca 30-60, pinnatifida 20-50, spirulina 1-15. Injecting 5-hydroxytryptamine to the head or abdominal feet of the parti-colour abalone, and making the lay eggs and discharge spermium synchronous with a different geographical parti-colour abalone. Making artificial insemination and getting filial generation. and guaranteeing the survival rate during the line haul, making a two species parent parti-colour abalones mature in a same time by using reasonable bait breeding; Using ejecting 5-hydroxytryptamine to make the gamete discharge in a same time and overcome the physiological difference of gamete discharging and sexual maturity because of geographic isolation of different geographical species, and making the gonadal mature and gamete discharge at the same time.

Description

technical field [0001] The invention relates to a method for genetic improvement of variegated abalone, in particular to a method for hybridizing variegated abalones of different geographical populations. Background technique [0002] At present, abalone seedlings are produced in the form of self-propagation of Taiwanese populations. The disadvantages of this production method are: inbreeding leads to trait decline, resulting in poor disease resistance of abalone seedlings, low survival rate, and frequent large-scale deaths. It is an effective way to improve stress resistance and production performance by using the method of crossing between different populations to take advantage of its heterosis. Pedro Cruz et al. used different scallop populations for crossbreeding, and the results showed that the hybrid group at the larval stage showed significant heterosis in both growth and survival (PedroCruz, Ana M Ibarra.Larval growth and survival of two catarina scallop (Argopecten...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D19/00A01K61/00
CPCY02A40/81
Inventor 柯才焕游伟伟
Owner XIAMEN UNIV
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