Preparation method of composite bone tissue engineering stent material

A technology of bone tissue engineering and scaffold material, which is applied in the field of bone tissue engineering in the medical field and the preparation of chitosan/silk fibroin/hydroxyapatite bone tissue engineering scaffold material, can solve the problem of mild reaction conditions of the preparation method and scaffold material. Unable to meet the requirements and other problems, to achieve the effect of mild reaction conditions, inhibition of bone resorption process, and enhancement of ALP activity

Active Publication Date: 2019-05-17
HOSPITAL OF STOMATOLOGY CHINA MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The present invention aims at the problems existing in the above-mentioned prior art, and provides a preparation method of a composite bone tissue engineering scaffold material, which solves the problem that a single scaffold material in the prior art cannot meet the requirements; and the preparation method of the present invention has mild reaction conditions , simple process and low cost

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  • Preparation method of composite bone tissue engineering stent material
  • Preparation method of composite bone tissue engineering stent material
  • Preparation method of composite bone tissue engineering stent material

Examples

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Effect test

Embodiment 1

[0029] A preparation method for a composite bone tissue engineering scaffold material, comprising the steps of:

[0030] Step 1), the preparation of the hydroxyapatite of zinc ion sustained release:

[0031] Get 0.2109 grams of calcium chloride and 0.0136 grams of zinc chloride and dissolve them in 25 milliliters of deionized water, slowly add 15 milliliters of creatine phosphate solution with a mass concentration of 2.5% at a rate of 40-60 drops / min, stir and mix Evenly, microwave at 100 degrees, heat for 10 minutes, stir at room temperature at 150-220r / min for 2-3h, adjust the pH of the reaction solution to 9-10 after stirring, sink in a water bath at the reaction temperature for 24-48h, and vacuum filter. Rinse three times with deionized water to obtain zinc ion slow-release hydroxyapatite;

[0032] Step 2), adding the zinc ion slow-release hydroxyapatite obtained in step 1) into the chitosan solution, stirring and mixing evenly and adjusting the pH value to 8.0 to obtain ...

Embodiment 2

[0038] A preparation method for a composite bone tissue engineering scaffold material, comprising the steps of:

[0039] Step 1), the preparation of the hydroxyapatite of zinc ion sustained release:

[0040] Dissolve 0.1 gram of calcium chloride and 0.01 gram of zinc chloride in 20 milliliters of deionized water, slowly add 10 milliliters of creatine phosphate solution with a mass concentration of 2.5% at a rate of 40 drops / min, stir and mix evenly during the period, Microwave at 100 degrees, heat for 10 minutes, stir at room temperature at 150-220r / min for 2 hours, adjust the pH value of the reaction solution to 9-10 after stirring, sink in a water bath at the reaction temperature for 24-48 hours, vacuum filter, and use deionized water Rinse three times to obtain zinc ion slow-release hydroxyapatite;

[0041] Step 2), adding the zinc ion slow-release hydroxyapatite obtained in step 1) into the chitosan solution, stirring and mixing evenly and adjusting the pH value to 8.0 to...

Embodiment 3

[0046] A preparation method for a composite bone tissue engineering scaffold material, comprising the steps of:

[0047] Step 1), the preparation of the hydroxyapatite of zinc ion sustained release:

[0048] Get 0.5 grams of calcium chloride and 0.03 grams of zinc chloride and dissolve them in 30 milliliters of deionized water, slowly add 20 milliliters of creatine phosphate solution with a mass concentration of 2.5% at a rate of 60 drops / min, stir and mix evenly during the period, Microwave at 100 degrees, heat for 10 minutes, stir at room temperature at 150-220r / min for 3 hours, adjust the pH of the reaction solution to 9-10 after stirring, sink in a water bath at the reaction temperature for 24-48 hours, vacuum filter, and use deionized water Rinse three times to obtain zinc ion slow-release hydroxyapatite;

[0049] Step 2), adding the zinc ion slow-release hydroxyapatite obtained in step 1) into the chitosan solution, stirring and mixing evenly and adjusting the pH value ...

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Abstract

The invention relates to a preparation method of a composite bone tissue engineering stent material. The preparation method comprises the following steps: 1) preparing zinc ion sustained-release hydroxyapatite; 2) adding the zinc ion sustained-release hydroxyapatite obtained in the step 1) to a chitosan solution, stirring and mixing uniformly and adjusting the pH to obtain a chitosan/hydroxyapatite suspension; 3) slowly adding the suspension obtained in the step 2) to a silk fibroin solution to obtain a mixture; 4) injecting the mixture obtained in the step 3) into a mold, conducting freeze drying, immersing the mold in an anhydrous methanol and sodium hydroxide solution h, rinsing with deionized water and conducting freeze drying again to obtain a final composite stent material. The composite bone tissue engineering stent material has good biocompatibility, degradable controllability and good formability, and can be used for treating bone defects.

Description

technical field [0001] The invention relates to a scaffold material for bone tissue engineering, in particular to a preparation method of a chitosan / silk fibroin / hydroxyapatite bone tissue engineering scaffold material compounded with zinc ions, which belongs to the bone tissue engineering in the medical field. Background technique [0002] Bone tissue destruction is very common in periodontal disease, and bone grafting is often required for bone reconstruction and healing. An ideal bone graft material can rapidly promote the formation of new bone without causing obvious adverse reactions to surrounding tissues and the whole body. At present, bone graft materials can be divided into autologous bone graft, allogeneic bone graft and non-bone material graft. Autologous bone transplantation is known as the "gold standard". The patient's autologous bone can come from the patient's tooth extraction wound or alveolar ridge in the edentulous area, without immune rejection, but the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/22A61L27/20A61L27/02A61L27/12A61L27/50A61L27/56A61L27/58
Inventor 李琛何晓宁孙进杰潘亚萍林莉喻施文
Owner HOSPITAL OF STOMATOLOGY CHINA MEDICAL UNIV
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