272 results about "Lentinula edode" patented technology

The invention discloses a label cleaning type crispness increasing method for fried food, and belongs to the field of fried products. According to the preparation method, firstly, lentinus edodes stem nanocellulose is prepared by combining enzymolysis with a high-pressure homogenization method, and the lentinus edodes stem nanocellulose is compounded with casein, so that stable lentinus edodes stem nanocellulose-casein gel emulsion can be prepared; then the gel-like emulsion is applied to the paste-coated fried product, and the gel-like emulsion is used for forming pores in advance in the paste so as to achieve the crispness increasing effect, reduce the oil content and inhibit grease oxidation. The stable gel emulsion based on a natural formula can replace a commercial leavening agent to realize clean label type crispness enhancement, and the prepared paste can be applied to various paste fried foods and has a good market prospect.

The invention provides a method for preparing an edible mushroom culture medium from agricultural waste biomass and application, and belongs to the technical field of agricultural resource utilization and edible mushroom culture. The edible fungus culture medium provided by the invention is prepared from the following components in parts by mass: 10 to 20 parts of wood dust, 15 to 30 parts of sawdust, 15 to 30 parts of corn straw, 20 to 30 parts of algae waste, 30 to 50 parts of cottonseed hull, 3 to 10 parts of chitosan, 3 to 10 parts of humic acid, 1 to 3 parts of gypsum, 1 to 3 parts of lime and 0.3 to 1 part of compound strain. By adopting the edible mushroom culture medium disclosed by the invention, the yield and the quality of mushrooms can be remarkably improved, and the quality of the mushrooms is effectively improved due to the addition of the algae waste, the humic acid and the chitosan. Meanwhile, the edible mushroom culture medium can also be used for cultivating other types of edible mushrooms such as oyster mushrooms and pleurotus eryngii, and the yield of the edible mushrooms is increased.

A Lentinula edodes-derived saltiness enhancing peptide, a method for preparing the same and use thereof are provided herein, belonging to the technical field of active peptides. The Lentinula edodes-derived saltiness enhancing peptide is derived from an enzymolysis extract of a Lentinula edodes fruiting body by flavourzyme and shows a strong ability to enhance saltiness. Compared with the saltiness value of a 4.0 g / L NaCl solution, the Lentinula edodes-derived saltiness enhancing peptide could replace about 50% of NaCl, thus reducing salt without reducing saltiness. Moreover, the Lentinula edodes-derived saltiness enhancing peptide also has a high umami value and can be used in preparation of low-salt foods including low-salt and flavor-enhancing foods.

The invention discloses microbial combined bacteria and a method for improving fertility of newly-cultivated land. The method comprises the following steps: combining every two of bacillus amyloliquefaciens WK1, white rot fungi NDM3-2 and trichoderma helichum HJR7 to form three combined bacteria; the three combined bacteria are applied to soil containing straw and lentinus edodes residues respectively, and a pot experiment (rice and white clover crop rotation) for two years is carried out with no addition of any bacteria as a contrast; analyzing the physical and chemical properties of the soil and the microbiological indexes of the soil; and according to an analysis result, the combination of the white rot fungi NDM3-2 and the trichoderma helichum HJR7 is confirmed to be an ideal scheme for improving the fertility of the newly-cultivated land.

The invention provides an influenza vaccine adjuvant based on lentinan as well as a preparation method and application thereof. The active ingredient of the vaccine adjuvant is lentinan, the lentinan is extracted from fresh lentinus edodes through a water extraction and alcohol precipitation method, no additional chemical modification is needed, the vaccine adjuvant can be used for preparing the influenza virus vaccine, and the influenza virus vaccine at least contains one of three inactivated influenza virus antigens, namely H1N1 A, H3N2 A and Victoria B. The invention also provides a vaccine preparation and a preparation method thereof, the vaccine preparation comprises the vaccine adjuvant and an influenza virus vaccine, the content of lentinan in the vaccine preparation is 4 mg / mL, and the vaccine preparation has an immunological enhancement effect.

The invention relates to the technical field of intelligent shiitake mushroom detection, and provides an intelligent shiitake mushroom detection system and method based on a deep learning model. The lentinus edodes intelligent detection system based on the deep learning model comprises a shooting device used for lentinus edodes image acquisition; the illumination device is used for supplementing a light source and ensuring the mushroom image acquisition quality; and the server is electrically connected with the shooting device and is used for receiving and processing the shiitake mushroom image information from the shooting device so as to realize intelligent detection of the size, the quality and the maturity of the shiitake mushrooms. The lentinus edodes intelligent detection system based on the deep learning model can meet high-precision and multi-dimensional detection requirements of lentinus edodes, and the automation and intelligence level of lentinus edodes factory production is improved. In addition, the invention further provides a method applied to the mushroom intelligent detection system.

The utility model relates to a mushroom stick crushing device, and belongs to the technical field of edible mushroom processing. Comprising a rack, a smashing driving device is fixedly installed on the surface of the rack, the output end of the smashing driving device is in transmission connection with a smashing mechanism installed on the side face of the rack, the top of the smashing mechanism is communicated with a spiral feeder through a smashing feeding bin, and the top of the spiral feeder is connected with a feeding bin; and a bottom outlet of the crushing mechanism is communicated and connected with the spiral discharger.

The invention provides an agricultural waste composite modified edible fungus culture medium and a preparation method thereof. The agricultural waste composite modified edible fungus culture medium is prepared from the following components in parts by mass: 20 to 30 parts of corn straw, 15 to 25 parts of rice hull, 10 to 20 parts of sawdust, 25 to 35 parts of cottonseed hull, 5 to 10 parts of soybean meal, 0.5 to 2.0 parts of complex microbial inoculant, 3 to 8 parts of bentonite, 1 to 3 parts of chitosan, 1 to 2 parts of gypsum and 1 to 2 parts of lime. According to the invention, the complex microbial inoculants are adopted to carry out solid state fermentation modification on the basic mixture, bentonite and chitosan are compounded into the activated functional slurry, and through the synergistic effect of solid state fermentation and the functional slurry, the granular structure, water retention and air permeability of the matrix are fundamentally improved, and efficient activation and slow release of nutrients are realized; finally, the fruiting period of the shiitake mushrooms is shortened to be within 85 days, the yield is increased by 10% or above, the shiitake mushroom proportion is remarkably increased, and high-value utilization of agricultural waste is achieved.

The invention relates to the technical field of agricultural fertilizers, in particular to a shiitake mushroom residue functional proteoglycan organic fertilizer as well as a preparation method and an application method thereof. The organic fertilizer is prepared from 40-60% of functional proteoglycan, 15-30% of humic acid, 8-15% of monopotassium phosphate, 5-10% of charcoal and 5-10% of seaweed extract, the functional proteoglycan is prepared by mixing shiitake mushroom residues, oyster mushroom residues and agaric residues, the molecular weight of the functional proteoglycan is 5-50 kDa, and the functional proteoglycan contains a beta-1, 3 / 1, 6-glucan structure; the preparation method comprises the steps of mushroom dreg pretreatment, ultrasonic-assisted enzymolysis, multi-stage membrane separation, concentration and drying, formula mixing, granulation and packaging and the like. The organic fertilizer has dual functions of promoting plant growth and activating a plant immune system, can significantly improve the disease resistance and yield of plants, and improves the physicochemical properties of soil; the preparation process is environment-friendly, high in extraction efficiency and low in energy consumption; and the pH value of the product is adjustable.

The invention belongs to the technical field of biological medicines, and particularly relates to a nutritional composition as well as a preparation method, application and a product thereof. The nutritional composition provided by the invention consists of nicotinamide, B vitamins and a fungal polysaccharide compound, the vitamin B consists of vitamin B1, vitamin B2, vitamin B6, vitamin B12 and folic acid; the fungal polysaccharide compound is composed of a lucid ganoderma extract, a mushroom extract, an agaricus blazei murill extract, a hericium erinaceus extract, a coprinus comatus extract, a grifola frondosa extract and a cordyceps militaris extract. Western medicine source substances (nicotinamide and B vitamins) and traditional Chinese medicine source substances (fungal polysaccharide compounds) are combined, and the nutrition composition is prepared through combination of the Chinese medicine and the western medicine. The nutritional composition has remarkable effects of enhancing immunity, delaying senescence, resisting fatigue and resisting oxidation, and shows extremely wide application prospects in the field of preparation of health-care products, medicines and cosmetics.

The utility model discloses a watering device suitable for mushroom planting matrix, which comprises a bottom plate, a planting frame is mounted at the top end of the bottom plate, a plurality of groups of planting plates are mounted in the planting frame, a watering mechanism is further mounted at the top end of the bottom plate, the watering mechanism comprises a water tank, a water pump, a main pipe, a shunt pipe, branch pipes and a spray head, and a plurality of groups of branch pipes are mounted on one side of the main pipe. The branch pipes are in one-to-one correspondence with the planting plates, the branch pipes are arranged above the planting plates at the corresponding positions, and multiple groups of nozzles are mounted at the bottom ends of the branch pipes; water in the water tank is pumped by the water pump, conveyed into the main pipe, shunted into the branch pipes through the shunting pipes and sprayed onto the planting plate through the nozzles, so that the shiitake mushrooms on the planting plate are watered, the reciprocating mechanism is driven by the driving assembly to move, and the reciprocating mechanism drives the main pipe to reciprocate left and right; the branch pipes are driven to reciprocate left and right, so that the nozzles can perform relatively comprehensive watering operation on the shiitake mushrooms on the planting plate.

The utility model discloses a mushroom stick water retention film bag hole pricking device which comprises a mushroom stick fixing frame, a mushroom stick rotator, a hole pricking mechanism and a motor, the rotating ends of the mushroom stick rotator and the hole pricking mechanism on the same side are meshed through a gear, and the power output end of the motor is connected with the power input end of the mushroom stick rotator or the power input end of the hole pricking mechanism. The mushroom stick rotator drives the mushroom sticks placed in the mushroom stick fixing frame to rotate, and the hole pricking mechanism pricks holes in the mushroom sticks. According to the device, mushroom sticks can be fixed in the radial direction, axial movement is prevented, rotation of the mushroom sticks is not hindered, the mushroom sticks can rotate in the axial direction under the action of the mushroom stick rotator, meanwhile, the hole pricking mechanism pricks holes in the rotating mushroom sticks, during hole pricking, due to the fact that the mushroom sticks rotate, the pulling force of the hole pricking mechanism on the mushroom sticks is reduced, and the mushroom sticks can be easily pricked. And it is guaranteed that pricking holes are round and uniform, and the mushroom stick water retention film bag is prevented from being damaged.

The present application discloses a kind of lentinus edodes automatic sorting machine, it is related to lentinus edodes sorting field, including screening box, the aperture of screen hole gradually reduces several screen plate, feeding table, further include: mounting plate, it is fixedly installed in the bottom of screen plate;Through mechanism, it is set the outside of mounting plate, the through mechanism includes reciprocating screw rod rotationally connected between the inner wall of mounting plate, the outside transmission connection of reciprocating screw rod has connecting strip, the inner side of connecting strip is equipped with sliding slot, the inner side of sliding slot is vertically slidably connected with connecting plate.The lentinus edodes automatic sorting machine, connecting strip is driven to reciprocate by reciprocating screw rod, and in the process of connecting strip movement, by reciprocating lifting mechanism and reset spring cooperation drive jack to reciprocate lifting reset action, so that the lentinus edodes jammed in screen hole can be fully ejected, effectively avoid the situation that lentinus edodes is jammed in screen hole for a long time, leading to lentinus edodes screening efficiency reduces and screening effect is poor.

The present application relates to the technical field of biological medicine, and particularly relates to a method for extracting and determining selenoprotein from selenium-rich lycoperdon pygmaeum mycelium, wherein the lycoperdon pygmaeum mycelium fermented by a 20L biological culture tank is dried in a vacuum drying box, ground into standard powder through a 100-mesh sieve, 0.5g of the standard powder is weighed, a 0.05mol / L NaOH solution is added, ultrasonic-assisted extraction is performed, then centrifugation is performed at a speed of 3000r / min for 15min, the supernatant is adjusted to an isoelectric point, centrifugation is performed again to obtain a protein precipitate, and finally the protein precipitate is dried in a freeze dryer to obtain a protein sample. The present application is dedicated to using the selenium-rich lycoperdon pygmaeum mycelium as raw material, adopting ultrasonic extraction, optimally optimizing the extraction process of selenoprotein, quantitatively analyzing the selenium content in the protein, detecting the change of the type and quantity of amino acids before and after selenium enrichment through a thin layer chromatography test, and understanding the influence of selenium on the amino acids in the lycoperdon pygmaeum mycelium.

The invention provides lactobacillus delbrueckii subsp. Bulgaricus LB047 and application thereof in promoting calcium absorption and enhancing immunity, and belongs to the technical field of microorganisms. The invention provides lactobacillus delbrueckii subsp. Bulgaricus LB047, and the preservation number of the lactobacillus delbrueckii subsp. Bulgaricus LB047 is CGMCC No.30725. The invention further provides a preparation method for preparing mushroom peptide fermentation liquor by utilizing the lactobacillus delbrueckii subsp. Bulgaricus LB047, and application of the prepared mushroom peptide fermentation liquor in promoting calcium absorption and enhancing immunity.

The utility model discloses a shiitake mushroom stem-removing and sorting device, which relates to the shiitake mushroom processing technical field, and comprises a stem-removing mechanism and an anti-blocking screening mechanism, the anti-blocking screening mechanism is connected to the discharge end of the stem-removing mechanism, the stem-removing mechanism comprises a base, a conveying assembly is arranged on the base, a dial wheel assembly is arranged above the conveying assembly, and the anti-blocking screening mechanism is connected to the discharge end of the anti-blocking screening mechanism. A pressing wheel assembly is arranged on one side of the shifting wheel assembly, and a handle cutting assembly is arranged on the inner side of the conveying assembly. The anti-blocking type screening mechanism is connected to the discharging end of the stem removing mechanism, material transfer between original devices is omitted, the machining efficiency is effectively improved, meanwhile, the problem that mushroom stems are clamped in holes and cannot be completely lowered in place is solved through the pressing wheel assembly arranged above the conveying assembly, and the processing efficiency is improved. And the problem of blockage caused by the fact that the shiitake mushrooms are clamped in the screening holes is solved through the anti-blocking assembly.

The invention discloses a processing method of high-fiber steamed buns with a slow digestion function, and belongs to the technical field of food processing. The quality defects that traditional wheat steamed buns are poor in gluten network structure stability, prone to softening and collapsing, prone to aging and the like are overcome. According to the invention, a low-moisture extrusion technology is combined with compound enzyme directional modification to modify the dietary fibers in the lentinus edodes stems, so that the modified lentinus edodes stem dietary fibers are obtained. A traditional steamed bun processing technology and a traditional steamed bun processing formula are optimized, key technological parameters such as extrusion technological parameters, enzymolysis conditions and the addition amount of the modified dietary fibers are optimized, and the high-fiber steamed bun rich in the modified lentinus edodes stem dietary fibers is prepared. As an exogenous nutritional supplement and a texture modifier, the extruded modified dietary fiber not only solves the quality defects of poor gluten network structure stability, easiness in softening, easiness in aging and the like of the traditional wheat steamed bun, but also reduces the digestibility of the wheat steamed bun. The method has a wide application prospect for developing wheat flour derivative products with high fiber and low digestibility.

The utility model provides a kind of organic fragrant mushroom culture medium rapid inoculation equipment, including main body, feeding conveyor belt, inoculation post, feeding hopper, the side of feeding conveyor belt is provided with discharging conveyor belt, feeding conveyor belt and discharging conveyor belt between are provided with bagging mechanism, bagging mechanism includes the mounting bracket of rotation installation in the side of feeding conveyor belt, the side of mounting bracket is rotatably installed with driving gear ring, the outside of driving gear ring is engaged with installation and is equipped with multiple groups of driven gear ring.The utility model is cooperated with chuck by bagging mechanism, XY axis linear guide rail, expand the flange plate synchronous opening and closing by rack, the packaging bag is accurately adsorbed and is moved by cylinder drive chuck and is sleeved on the outer wall of flange plate, realize the automatic flanging and bagging of packaging bag, replace manual bagging operation, reduce manual intervention, significantly improve bagging efficiency;Conveying chain plate is separately arranged on the upper and lower sides of discharging conveyor belt, realize the continuous supply and recovery of packaging bag, match high-speed inoculation rhythm.

The application provides a roasted sesame salad dressing and a preparation method thereof, and relates to the field of condiments. The method comprises the following steps: taking egg yolk powder, white granulated sugar, vinegar, salt and mustard powder, adding water, and mixing uniformly to obtain a liquid 1; adding vegetable oil to the liquid 1, homogenizing to a paste, and obtaining a liquid 2; adding vinegar, monosodium glutamate, sesame fragments, soy sauce, lentinus edodes extract, yeast extract, white pepper powder, sucralose and water to the liquid 2, mixing uniformly, and obtaining a liquid 3; adding xanthan gum to the liquid 3, stirring, passing through a colloid mill, and obtaining a liquid 4; sterilizing the liquid 4 by heating while stirring, keeping warm, filling, and obtaining the roasted sesame salad dressing. The roasted sesame salad dressing prepared by using the optimized formula has the advantages of bright color, good and stable texture, rich taste, strong aroma, excellent sensory quality, and obviously reduced oil separation phenomenon when stored at room temperature, and the consumer experience is improved.

The invention discloses primers, a kit and a method for detecting four viruses in lentinus edodes. According to the invention, LAMP (loop-mediated isothermal amplification) primer groups are respectively designed and screened according to four viruses in the lentinula edodes, and the four viruses are respectively a Lentinula edodes dominant virus 1 virus, a Lentinula edodes dominant virus HKB virus, a Lentinula edodes dominant gene-like virus 1-Hunong-1 virus and a Lentinula edodes dominant virus 5 virus. The detection system is micro-quantized, the local concentration of the target sequence is improved from the side, the detection limit is greatly improved, and the lower detection limit can reach 10 copy / microliter.

The application discloses a novel plant protein meat strip and a preparation method thereof, and belongs to the technical field of food processing. The plant protein meat strip is prepared from the following raw materials in parts by weight: soybean protein isolate 30-40 parts, pea protein 20-25 parts, gluten powder 10-15 parts, lentinus edodes powder 8-12 parts, konjac powder 5-8 parts, corn starch 10-15 parts, sunflower seed oil 5-8 parts, yeast extract 2-3 parts, carrot powder 2-4 parts, celery powder 1-2 parts, salt 0.8-1.2 parts, ginger powder 0.5-1 part and garlic powder 0.5-1 part. The preparation method comprises the following steps: mixing ingredients, multi-section temperature control double-screw extrusion molding, low-temperature marinating using specific plant-based broth, drying and high-temperature sterilization. Through protein compounding and process optimization, the application solves the problems of poor texture and single flavor of the existing plant meat strips, the product has a taste close to real meat, the flavor is natural and mellow, and the product is suitable for industrial production.

The invention discloses a lentinus edodes MAPK family gene Le-10004182, and the sequence of the lentinus edodes MAPK family gene Le-10004182 is shown as SEQ ID NO. 1. An overexpression vector or an interference vector of the gene Le-10004182 is constructed through a cDNA (complementary deoxyribonucleic acid) sequence of the gene Le-10004182, and the overexpression vector or the interference vector is transferred into mushroom mycelia through an agrobacterium tumefaciens-mediated genetic transformation method, so that the biomass of the mushroom mycelia is regulated and controlled. The lentinus edodes MAPK family gene Le-10004182 and the regulation and control of the lentinus edodes MAPK family gene Le-10004182 on the growth of lentinus edodes mycelia provide a new solution thought for further analysis of the growth and development of the lentinus edodes mycelia and variety improvement.

The invention discloses a flavor enhancer with a compound flavor of morchella esculenta and shiitake mushrooms as well as a preparation method and a production line of the flavor enhancer. The flavor enhancer comprises the following raw material components in parts by mass: 10-50 parts of toadstool flavor powder, 5-40 parts of a toadstool Maillard reaction product, 0.08-8 parts of toadstool flavor peptide, 10-50 parts of shiitake mushroom flavor powder, 5-40 parts of a shiitake mushroom Maillard reaction product and 0.08-8 parts of shiitake mushroom flavor peptide. Morchella and shiitake mushrooms are cultured through liquid fermentation, toadstool umami substances are extracted through combination of enzymolysis and ultrasonic waves, toadstool umami peptides are prepared through a multi-step ultrafiltration method, and a Maillard reaction is conducted on toadstool fermentation liquor and shiitake mushroom fermentation liquor which are subjected to liquid fermentation culture; the shiitake mushroom umami substances are extracted through supercritical CO2, and shiitake mushroom umami peptides are prepared through a multi-step ultrafiltration method; all the components are mixed according to a proper proportion to form the flavor enhancer with the composite flavor. The problems that in the prior art, the extraction efficiency is low, active ingredients are prone to inactivation, and the flavor stability is poor are solved.

This invention discloses a compound traditional Chinese medicine powder inhaler for respiratory tract infections and its preparation method, relating to the field of pharmaceutical formulation technology. The compound traditional Chinese medicine powder inhaler for respiratory tract infections is composed of polysaccharide composite gel microspheres, forsythia, scutellaria, honeysuckle, and isatis root. The polysaccharide composite gel microspheres are composed of seaweed sulfated polysaccharide, lentinan, calcium chloride, and curcumin. Seaweed sulfated polysaccharide, lentinan, curcumin, and calcium chloride cross-link to form a three-dimensional network spherical structure, improving the particle morphology and flowability of the micropowder. Furthermore, due to its good hygroscopic and moisturizing properties, it competitively reduces the moisture absorption of the micropowder, preventing aggregation and further improving flowability. In addition, the gel microspheres have excellent adhesion, adhering to the infection site after entering the respiratory tract, reducing expulsion losses due to respiration, maintaining an effective drug concentration at the infection site, and improving therapeutic efficacy.

The present invention relates to a highly effective, broad-spectrum anti-plant viral disease pharmaceutical composition and its application. The active ingredients of the anti-plant viral disease pharmaceutical composition include chlorin iron and lentinan; wherein the weight ratio of chlorin iron to lentinan is 1:6000 to 4:1. When a certain concentration of the chlorin iron and lentinan pharmaceutical composition is applied to plants, the two active ingredients exert a synergistic effect, alleviating symptoms of diseased plants, reducing disease indexes, and reducing viral accumulation in the plants. The plant anti-viral disease pharmaceutical composition is a biological agent that is environmentally friendly, safe, and environmentally friendly. It has important application prospects in combating plant viral diseases, preventing and controlling plant diseases caused by plant viruses, and improving plant quality.

The invention relates to the technical field of edible mushroom cultivation, and discloses mushroom stick sealing equipment and a method thereof. The mushroom stick sealing equipment is characterized in that a storage box is arranged on a carrier plate, a charging pipe penetrating through the carrier plate is arranged at the bottom of the storage box, and a spiral feeding piece is arranged in the charging pipe; the sleeve is rotationally connected to the bottom of the carrying plate. The charging pipe is located in the sleeve. The opening part comprises a sleeve, a plurality of supporting rods and an opening and closing part, the sleeve is arranged on the charging pipe in a sleeving mode, the supporting rods are all hinged to the bottom of the outer wall of the sleeve, and the opening and closing part is connected with the supporting rods and used for driving the supporting rods to be opened or contracted; the driving piece is used for enabling the sleeve and the supporting rods to twist the mushroom stick bag for closing up; the sealing assembly is arranged on the side edge of the sleeve and used for sealing the closing position of the mushroom stick bag. The mushroom stick sealing equipment is simple and compact in structure, the sealing work can be completed without transferring the mushroom sticks, the situation that mushroom stick materials are spilled is avoided, the compactness of the mushroom sticks is guaranteed, and the whole equipment is convenient to produce and use and high in automation degree.

The utility model discloses a mushroom cultivation frame which comprises a frame body. The fixing rod is arranged between the frame bodies and fixedly provided with a containing face formed by a tangent face, a containing groove is formed in the upper surface of the containing face, the fixing rod is further provided with a water conveying channel, and the water conveying channel communicates with the containing groove. The water injection piece is installed in the containing groove and comprises an installation part and an insertion pipe, and the installation part is rotatably arranged in the containing groove. The insertion pipes capable of being contained in the fixing rods are arranged between the frame bodies, the holes are formed in the insertion pipes, water is evenly conveyed through the holes, meanwhile, when the insertion pipes are contained in the containing grooves, a plurality of fungus bags can be placed on the fixing rods, and meanwhile the protruding water injection piece hooks prevent the fungus bags from rolling between the fixing rods; and meanwhile, the fixing rod can be rotated, so that an up-down opposite structure is formed, mushroom bags placed between the upper fixing rod and the lower fixing rod can be well injected with water from top to bottom, and the water injection efficiency can be improved.

The invention relates to the technical field of food processing, and particularly discloses a method for resource utilization of lentinus edodes stipes, which is characterized in that a complex enzyme preparation consisting of cellulase, acid protease and pectinase is used for carrying out synergistic enzymolysis on the lentinus edodes stipes under the optimal enzymolysis conditions of pH 3.5, temperature 51 DEG C, enzyme concentration 6% and time 4 hours. According to the method provided by the invention, the hardness and chewiness of the lentinus edodes stipe can be remarkably reduced, the compact fiber structure of the lentinus edodes stipe can be destroyed, the release and content of bioactive components such as ergothioneine, soluble protein and crude polysaccharide can be synchronously increased, and the chocolate-coated lentinus edodes stipe developed based on the method is full in appearance, uniform in color and luster, coordinated in flavor, good in microbial safety and suitable for industrial production. And high-valued comprehensive utilization of shiitake mushroom processing byproducts is realized.

This invention provides a drug for antagonizing bacterial endotoxins, its preparation method, and its application, belonging to the biomedical field. The composition consists of 25-55 parts of dried powdered traditional Chinese medicine extract, 25-70 parts of mixed essential oil powder, and 40-90 parts of lentinan powder. The traditional Chinese medicine extract is prepared by decoction of Scutellaria baicalensis, Lonicera japonica, Forsythia suspensa, and Isatis indigotica under reduced pressure at low temperature and spray drying. The mixed essential oil powder is prepared by encapsulating peppermint oil and eucalyptus oil with gelatin and hydroxypropyl cellulose and then spray drying under high pressure. This invention, through specific formulation and processing, significantly enhances the synergistic antagonistic effect of each component against endotoxins. Experiments show that this composition exhibits antagonistic activity far superior to ibuprofen in vitro; in vivo, it significantly reduces the endotoxin content in the plasma of 817 broiler chickens, rapidly alleviates LPS-induced fever and diarrhea, and improves decreased growth performance. Therefore, this invention is of great significance for reducing antibiotic dependence and improving the efficiency of livestock and poultry farming.

The application discloses a tricholoma matsutake mushroom and vegetable fresh composite seasoning and belongs to the field of food seasoning processing. The seasoning contains 7-15 parts of edible mushroom composite enzymatic extract, 5-15 parts of laminaria enzymatic extract, 35-45 parts of edible salt and other raw materials in terms of mass fraction; the edible mushroom composite enzymatic extract and the laminaria enzymatic extract are respectively prepared through a two-step enzymatic process, and the edible mushroom contains multiple rare mushroom species such as tricholoma matsutake and lentinus edodes. The seasoning takes rare edible mushroom and laminaria as the umami core, and is compounded with kale powder and fruit and vegetable powder, is rich in flavor and nutrition, and has the functions of seasoning and nutrition supplementing; the umami synergy is realized through the enzymatic process, the umami strength is high, and the taste is mellow; the raw materials are natural and free of chemical synthetic umami agents, the utilization rate of the raw materials is high, the clean label trend is met, the seasoning can be applied to the field of high-end soup bases and the like, and the product has high added value.