The invention discloses an oyster mushroom cultivation material. The cultivation material is characterized by using hickory branch chips, nutshell chips and husk chips as main raw materials, and using mushroom dreg, bran, calcium superphosphate, gypsum powder and the like as auxiliaries, wherein the water content is 63-65%. The invention further discloses a manufacturing method of the cultivation material. The manufacturing method and the cultivation material have the following advantages: 1, the hickory branch chips, the nutshell chips and the husk chips are insolated by sunshine, secondarily fermented or piled up to be aged, so that the toxic bacteriostats are degraded, and the macromolecular substances such as lignin and cellulose are decomposed to simple substances which are beneficial to absorb by oyster mushroom hypha, and the growth of the oyster mushroom hypha can be promoted; 2, the cultivation material proportion is reasonable, the bacterial material is good in breathability, the water-retaining property is strong, the effective mushroom quantity is large, the yield is high, and the biological efficiency is up to 130.3-142.6%; 3, the preparation method provides a solution for innocent treatment of hickory branch, nutshell and husk, and provides a new substrate for cultivating the oyster mushroom.