23 results about "Biological efficiency" patented technology

The invention discloses a Lentinus edodes strain SICAU-F2-1283 and application thereof, the strain is preserved in China Center for Type Culture Collection on April 14, 2025, the preservation address is No.3, Yard 1, Beichen West Road, Chaoyang District, Beijing, and the preservation number is CGMCC No.41891. The Lentinus edodes strain SICAU-F2-1283 has been preserved in China Center for Type Culture Collection. The lentinus edodes strain provided by the invention has the advantages of more fruiting quantity in a wheat straw culture medium, large single mushroom mass, high biological efficiency and excellent agronomic traits. The new mushroom variety provided by the invention solves the problems of low yield and poor quality of mushrooms in wheat straw cultivation, provides choices of more varieties of different types for the edible mushroom industry, and has a remarkable application prospect in the field of mushroom planting.

The invention discloses a pholiota adipose strain HK6, and belongs to the technical field of edible fungus research. The preservation number of the pholiota adipose strain HK6 is CGMCC (China General Microbiological Culture Collection Center) NO.41675, the pholiota adipose strain HK6 is named as pholiota adipose by taxonomy, the preservation date is November 28, 2024, and the preservation address is Institute of Microbiology, Chinese Academy of Sciences, No. 3, Beichen West Road, Chaoyang District, Beijing. The space mutation pholiota adipose strain HK6 is obtained through screening, the biological efficiency of the space mutation pholiota adipose strain HK6 reaches 82%, the length of the stipe is long, the space mutation pholiota adipose strain HK6 is suitable for industrialized production, the space mutation technology enriches germplasm resources of pholiota adipose, the pholiota adipose strain suitable for industrialized production is obtained, and a new thought is provided for breeding of new edible fungus varieties.

The invention relates to an organic edible fungus culture medium and application thereof, and the organic edible fungus culture medium comprises the following components in percentage by mass: 20-45% of sawdust, 45-65% of corncob, 1-15% of tenebrio molitor manure and 1-10% of shell powder calcined product. The organic edible mushroom culture medium is prepared by scientifically compounding sawdust, corncobs, tenebrio molitor dung and shell powder calcined products, acidification of the culture medium can be effectively inhibited, the mushroom stick pollution rate is reduced, rich nutrient substances are provided for edible mushrooms, the yield and quality of organic edible mushrooms are remarkably improved, the biological efficiency is high, and the nutritive value and commercial value are high; raw materials are simple and easy to obtain, waste recycling can be achieved, and the cost of the culture medium is reduced; the method is used for field production to improve quality and yield stability.

The present invention relates to the field of bio-microbial consortium technology, and specifically to a heavy oil bio-microbial consortium recycling system. The system includes a microbial consortium screening module, a reaction delay identification module, a structural fatigue assessment module, a proliferation behavior calculation module, and a microbial replacement recommendation module. The present invention improves the efficiency and economy of heavy oil biodegradation by screening microbial consortia adapted to different heavy oil environments and optimizing their recycling. By obtaining detailed status parameters of heavy oil samples and dividing the microbial culture environment, the metabolic capacity and environmental adaptability of the bacterial strains can be accurately assessed. The start-up delay time and product concentration peak of the microbial consortium degradation reaction can be monitored, and strains with high response efficiency can be identified. The microbial consortium structure is optimized and the response speed of the biodegradation process is increased, thereby enhancing the biological efficiency of heavy oil treatment. Furthermore, a detailed assessment of strain structural fatigue and proliferation behavior is performed, enabling dynamic optimization of microbial consortium iteration.

The invention belongs to the technical field of microorganisms, and discloses a pleurotus purpureus strain 162 and a cultivation method thereof. The preservation number of the strain 162 is CGMCC (China General Microbiological Culture Collection Center) No. 42041. The cultivation method comprises the following steps: 1) inoculating a pleurotus purpureus strain 162 on a mother strain culture medium to obtain a strain 162 mother strain; 2) inoculating the strain 162 mother strain to a stock culture medium to obtain a strain 162 stock; 3) inoculating the strain 162 stock seed to a cultivar culture medium to obtain a strain 162 cultivar; and 4) cultivating the cultivar of the strain 162 to obtain the sporocarp of the strain 162. The strain 162 is fast in hypha growth, short in after-ripening period, fast in fruiting, white in pileus, strong in adaptive capacity and short in harvesting period; the biological efficiency is high; the feed is high in nutritional value and has strong disease resistance to bacterial brown spot.

The present application relates to the technical field of Volvariella volvacea cultivation, and particularly relates to a Volvariella volvacea cultivation substrate and a cultivation method. The preparation raw material of the Volvariella volvacea cultivation substrate comprises the following components in parts by weight: extract / pectin@chitosan microspheres 3-5 parts, modified tannic acid-dextran 5-7 parts, rice straw 30-40 parts, corn cob 10-15 parts, bran 4-6 parts, and corn flour 3-5 parts. The present application utilizes the slow-release effect of the microspheres and the water storage network of the modified tannic acid-dextran to achieve synergistic effect, significantly improves the water retention rate, mycelial growth rate and biological efficiency of the cultivation substrate, and realizes high-yield and high-quality cultivation of Volvariella volvacea.

The invention relates to a white flammulina velutipes F523 with the preservation number of GDMCC No.67335. The white flammulina velutipes F523 is high in biological efficiency, short in growth cycle and stable in strain cultivation character, can be stably inherited and is used for flammulina velutipes cultivation and breeding. The invention also relates to an identification method of the flammulina velutipes F523, which comprises the following steps: by taking the DNA of the flammulina velutipes mycelium to be detected as a template, carrying out PCR (Polymerase Chain Reaction) amplification by adopting the primer combination 1 and / or the primer combination 2, and detecting whether the amplification product has a band with a specific size so as to identify the F523. The screening method is simple to operate, and detection can be performed only by using mycelia; the detection time is only 2-3 hours, so that the breeding efficiency is improved, and the cost is greatly saved.

The invention belongs to the technical field of edible fungus cultivation. The invention relates to an auricularia polytricha strain, in particular to a Jiangxi auricularia polytricha strain and a breeding method thereof. The invention provides a novel Auricularia polytricha (Auricularia polytricha) strain 'Jiangxi' (Auricularia polytricha) and an efficient breeding technology system of the novel Auricularia polytricha (Auricularia polytricha) strain. The strain is stable in character and high in auricularia polytricha fruiting capacity, and systematic artificial cultivation of auricularia polytricha is achieved for the first time. According to the invention, a liquid culture medium is optimized, so that the mycelium pellet density and biomass of the strain are respectively increased by 62.65% and 55.06%, and elaeagnus pungens, pterocarya stenoptera, ligustrum lucidum ait and sophora japonica are screened as optimal matrixes through cut-log cultivation; a high-yield bag cultivation medium is developed aiming at the strain, the biological efficiency is 75-83%, and the total yield of the third-generation fresh ears is about 130g / bag. The method breaks through the bottleneck that auricularia polytricha depends on wild collection, provides germplasm and technical support for industrialization, and has good application value.

The invention relates to the field of edible mushroom substrates, in particular to a treatment method for improving the utilization rate of an edible mushroom substrate. The method comprises the following steps: uniformly spraying a chemical reagent solution on lignocellulose, compacting and granulating, and carrying out stacking reaction to obtain a lignocellulose material; and mixing the lignocellulose material with other auxiliary materials to prepare the edible mushroom substrate for edible mushroom cultivation. According to the method, chemical reagents are added, densification granulation treatment is combined, and the enzyme digestibility of the lignocellulose material is remarkably improved by destroying a complex lignocellulose structure in the lignocellulose raw material, so that hyphae of edible fungi more easily intrude into lignocellulose. According to the method, the cooperative relationship between the density of densification granulation treatment and the stacking time is further optimized, the stability of the physical structure of the edible mushroom substrate is improved, the utilization rate of the edible mushroom substrate is further improved, and finally the yield and the biological efficiency of the edible mushrooms are remarkably improved.

The invention discloses an agricultural waste straw double-base-material resource utilization method, and belongs to the technical field of agricultural waste resource utilization. The modified magnetic straw carbon has the functions of adsorption, catalysis and magnetic recovery, a closed-loop utilization mode of straw-edible fungus base material-fungus residue-seedling raising matrix is constructed, and the bottleneck problems that straw is difficult to degrade and the utilization rate of fungus residue is low are solved. The method effectively improves the comprehensive utilization rate of agricultural wastes, double base materials can significantly improve the biological efficiency of edible mushrooms and improve the survival rate of rice seedlings, and the method has significant economic and ecological benefits and is suitable for large-scale agricultural popularization and application.

The invention provides a thin-skin cheese bacterium strain, which is a thin-skin cheese bacterium V11, and the preservation number of the thin-skin cheese bacterium strain is GDMCC (China General Microbiological Culture Collection Center) NO.65077. The invention further provides a preparation method of the thin-skin cheese bacterium strain. The invention also provides a cultivation method of the thin-skin keromycetes, which comprises the steps of culture material pretreatment, bagging and sterilization, inoculation and fungus cultivation, after-ripening culture, bud pressing and fruiting, fruiting management and the like, so as to obtain sporocarp of the thin-skin keromycetes, then harvest and post-harvest management are carried out, and then secondary fruiting is carried out. The thin-skin cheese bacterium strain can produce two times of mushrooms, the yield of sporocarp is 196.80 + / -5.21 g / bag, the biological efficiency is 35.14 + / -0.93%, the total flavonoid content of sporocarp is 708 mg / 100 g, the crude protein content is 16.30 g / 100 g, the dietary fiber content is 18.82 g / 100 g, the calcium content is 554 mg / kg, the degradation rate of the strain to matrix lignin is 33.93%, the degradation rate of cellulose is 37.72%, and the degradation rate of hemicellulose is 28.96%.

The invention relates to the technical field of biological flora, in particular to a thickened oil biological flora recycling system which comprises a flora screening module, a reaction delay recognition module, a structural fatigue evaluation module, a proliferation behavior calculation module and a thallus replacement suggestion module. According to the method, the efficiency and economical efficiency of heavy oil biodegradation are improved by screening the microbial flora adapting to different heavy oil environments and optimizing and recycling the microbial flora, and the metabolic capability and environmental adaptability of strains can be accurately evaluated by obtaining detailed state parameters of the heavy oil sample and dividing the thallus culture environment; according to the method, the starting delay time and the product concentration peak value of a flora degradation reaction are monitored, efficient response strains are recognized, the flora structure is optimized, the response speed of the biodegradation process is increased, the biological efficiency of heavy oil treatment is improved, fatigue and proliferation behaviors of the strain structure are carefully evaluated, and dynamic optimization of flora iteration is achieved.

The invention discloses a pholiota abietis ZJLS001 and a cultivation method thereof. The classification name of the pholiota abietis is that the preservation number of the pholiota abietis is CGMCC (China General Microbiological Culture Collection Center) NO.41797, and in the cultivation process, the proportion of a cultivation bag in which the pholiota abietis ZJLS001 does not run spawn due to contamination of infectious microbes is 0; 5-9 days after mycelium stimulation, a pale yellow rice-grain primordium is formed, the average bud number of the primordium is 28, and the average diameter of the primordium is 1-2 mm; 290-350 g of fresh mushrooms are produced by two crops of the pholiota abies ZJLS001, and the biological efficiency is 82-87.5%. The strain is suitable for a liquid strain inoculation cultivation artificial propagation method, the cultivation method is simple and convenient, and the strain has the advantages of being fast in spawn running, little in pollution and strong in hypha; sporocarp is thick in pulp, yellow in color and stable in character; the strain has the advantages of high yield, realization of large-scale and industrial cultivation, high application value, short production period, low cost, safety and reliability.

The invention discloses a novel schizophyllum commune strain and an artificial cultivation method thereof, the optimum carbon source of schizophyllum commune hyphae is cane sugar, the optimum nitrogen source is yeast extract powder, the optimum pH is 5.0, the optimum temperature is 32 DEG C, optimized 78% of mulberry sawdust, 20% of wheat bran, 1% of lime and 1% of gypsum are used as matrix cores, and a low-temperature induction and constant-temperature high-humidity fruiting mode is matched, after inducing for about 20 days at the low temperature of 18 DEG C, managing fruiting at the temperature of 25 DEG C and the relative humidity of 90%-95%, and realizing the high-yield stable performance that the average is 133.39 g / bag and the biological efficiency is 66.70%. Compared with the prior art, the method has the advantages that the method is standardized, can be copied, is suitable for large-scale and factory popularization, can effectively solve the problems that the traditional schizophyllum commune cultivation is unstable in yield and low in efficiency, and has remarkable industrial application value.

The present invention provides a Lepista sordida strain, named G-0011, with a preservation number of CGMCC NO. 41166, preserved by the General Microbiological Center of the China Committee for Culture Collection of Microorganisms on February 5, 2024. The content of total flavonoids in the fruiting body of the Lepista sordida strain is 5.530±0.004mg·g<supgt;−1< / supgt>. The cultivation method of the fruiting body of the above Lepista sordida strain is as follows: The Lepista sordida strain G-0011 is cultured successively through the stages of inoculation, spawn-running, and fruiting to obtain the fruiting body of Lepista sordida. The Lepista sordida strain G-0011 provided by the present invention has stable heredity. The flavonoid content in the fruiting body of Lepista sordida obtained by culturing this strain can reach 5.530±0.004mg·g<supgt;−1< / supgt>, and this strain has the potential for commercial production, with a yield of 4.62±0.17kg·m<supgt;‑2< / supgt> and a biological efficiency of 35.51±1.29%. It has a strong ability in synthesizing total flavonoids and can be used in the preparation of antioxidant health foods.

The invention discloses a hymenopellis raphanipes mutant strain M1 obtained through artificial mutagenesis, the hypha growth rate of the hymenopellis raphanipes mutant strain M1 is larger than or equal to 5.20 mm / d, the biological efficiency reaches 53.6%, the hymenopellis raphanipes mutant strain M1 has strong resistance to trichoderma harzianum, the proportion of first-grade mushrooms is larger than or equal to 40.6%, and the hymenopellis raphanipes mutant strain M1 is good in hereditary stability. The invention also provides a breeding method of the strain, a special liquid fermentation culture medium and an industrial cultivation process in an intelligent mushroom square cabin. The method solves the problems that traditional cultivation is long in period, high in pollution rate, difficult to factorize and the like, and a complete solution is provided for annual efficient production of oudemansiella oosporium.

This invention discloses a method for liquid culture of *Armillaria mellea* fruiting bodies, mycelial colonies, and mycelial cords. The culture method employed in this invention shortens the exposure time of the mycelial colony, reduces contamination by other microorganisms, increases aeration, and promotes the differentiation, formation, and development of *Armillaria mellea* fruiting body primordia. After the fruiting bodies mature, the three-component products of fruiting bodies, mycelial colonies, and mycelial cords can be harvested, with a total biological efficiency of up to 296%. This cultivation method achieves unparalleled biological efficiency compared to other methods, produces no waste, and yields higher production and economic benefits.

A low-cost high-quality tremella aurantialba cultivation method is characterized in that composite edible mushroom residues are added into a cultivation material, the composite edible mushroom residues comprise, by mass, 40-60% of Schizophyllumcommuneh fermentation mushroom residues, 20-30% of oyster mushroom residues and 20-30% of shiitake mushroom residues, and the mass ratio of the composite edible mushroom residues in the cultivation material is 50-58%. Schizophyllumcommuneh is adopted to be subjected to pretreatment and fermentation to prepare the fermentation mushroom residues, then the fermentation mushroom residues are mixed with the shiitake mushroom residues and the oyster mushroom residues according to a specific proportion, the mixture serves as a main material to replace cotton seed hulls in a conventional cultivation material in a large proportion, the production cost is effectively reduced, meanwhile, the yield of tremella aurantialba is increased, and the quality of tremella aurantialba is improved; the biological efficiency of the tremella aurantialba cultivated by adopting the cultivation material reaches 80% or above, and the contents of polysaccharides, amino acids, ergothioneine and other nutritional ingredients reflecting the quality of the tremella aurantialba in the tremella aurantialba are all increased. While the cost is reduced, the yield and the quality of the tremella aurantialba are improved, and the economic benefit of factory tremella aurantialba cultivation is effectively improved in a tradeoff and tradeoff mode.

The invention discloses a cultivation medium formula and a cultivation method of novel panus hirsutus, the cultivation medium comprises the following components by mass percentage: 50% of cottonseed hull, 28% of sawdust, 20% of bran, 1% of gypsum, and 1% of lime, and the water content of the cultivation medium is adjusted to about 60%. The novel panus hirsutus cultivation method adopting the cultivation medium formula comprises the steps of cultivation bag manufacturing, inoculated cultivation, fruiting management, harvesting and the like. The formula is scientific and reasonable, raw material sources are wide, cost is low, and cultivation method operation is standard; by adopting the formula and the method, the growth speed of the new panus gallica hyphae is high, the number of formed primordia is large, the single-bag yield of the sporocarp is as high as 55.75 g, the biological efficiency reaches 13.94%, the sporocarp is full in shape and compact in texture, the problems that raw material selection is lack of basis and the yield fluctuation is large in the large-scale cultivation of the new panus gallica are effectively solved, and the large-scale cultivation of the new panus gallica is realized. Reliable technical support is provided for industrial development and popularization, and remarkable economic, social and environmental benefits are achieved.

The present invention provides a Tyromyces chioneus strain, which is Tyromyces chioneus V11 and has a deposit number of GDMCC No. 65077. The present invention also provides a cultivation method for the Tyromyces chioneus, comprising the steps of pre-treatment of culture medium, bagging and sterilization, inoculation and cultivation, post-ripening culture, bud-inducing and fruiting, and fruiting management, to obtain Tyromyces chioneus fruiting bodies, followed by harvesting and post-harvest management, and secondary fruiting. The *Cephalotaxus fortunei* strain of this invention can produce two flushes of mushrooms, with a fruiting body yield of 196.80±5.21 g / bag, a biological efficiency of 35.14±0.93%, a total flavonoid content of 708 mg / 100g, a crude protein content of 16.30 g / 100g, a dietary fiber content of 18.82 g / 100g, and a calcium content of 554 mg / kg. The strain exhibits a lignin degradation rate of 33.93%, a cellulose degradation rate of 37.72%, and a hemicellulose degradation rate of 28.96%.

This technical solution belongs to the field of edible and medicinal fungi cultivation technology. In order to stabilize production, shorten the cycle, increase yield, and thus effectively realize the year-round industrialized cultivation of Sanghuang and the standardized and regulated production system of the entire green and safe Sanghuang industry chain, and further enhance the level and influence of the Sanghuang industry, this invention provides a double-layer superimposed substrate cultivation medium for Sanghuang and its application. The medium consists of two specific substrates forming a double-layer superimposed medium. The upper substrate components include mulberry wood chips, oak wood chips, cottonseed hulls, wheat bran, white sugar, gypsum, lime, perlite, corn cob, and water. The lower substrate specific components include mulberry wood chips, fruit tree branch wood chips, cottonseed hulls, wheat bran, white sugar, gypsum, lime, corn cob, and water. After application through steps such as seed production, medium preparation, inoculation and cultivation, yellowing management, and harvesting, significant technical effects have been unexpectedly achieved, such as a fresh weight of over 71g / bag, a biological efficiency of over 16%, and an overall cultivation cycle as short as 94 days.

The application discloses a pholiota adiposa strain HK6 and belongs to the technical field of edible fungi research.The pholiota adiposa strain HK6 has a preservation number of CGMCC NO.41675, a taxonomic name of pholiota adiposa, a preservation date of November 28, 2024, and a preservation address of No.3, Institute of Microbiology, Chinese Academy of Sciences, No.1, Beichen West Road, Chaoyang District, Beijing.The application screens a spaceflight mutagenesis pholiota adiposa strain HK6, the biological efficiency of which reaches 82 %, the length of the stem is long, and the strain is suitable for factory production, the spaceflight mutagenesis technology enriches the germplasm resources of the pholiota adiposa, and the pholiota adiposa strain suitable for factory production is obtained, thereby providing a new idea for breeding of new varieties of edible fungi.

The invention discloses a culture medium for ganoderma sinense cultivation and a preparation method and application thereof, and belongs to the technical field of edible fungus cultivation. The culture medium is prepared from the following main materials in percentage by weight: 13 to 20 percent of couch grass, 28 to 35 percent of arundo fungus grass and 40 to 45 percent of dicranopteris pedata, and the following auxiliary materials in percentage by weight: 0.1 to 0.8 percent of brown sugar, 0.8 to 1.8 percent of gypsum powder, 0.7 to 1.8 percent of urea, 0.2 to 1.2 percent of monopotassium phosphate, 0.05 to 0.16 percent of shell powder, 0.9 to 2.3 percent of corn flour and 0.02 to 0.07 percent of phoenix mushroom essence. The preparation method comprises the following steps: uniformly mixing the main material and the auxiliary material respectively, totally mixing, adjusting the water content to 45-65% and the pH value to 5-7, bagging, sealing, and sterilizing by high-pressure steam to obtain the sterile culture medium. The culture medium is used for cultivation of ganoderma sinense, the biological efficiency can reach 4.98 + / -1.07%, the polysaccharide content of sporocarp can reach 635 mg / 5 g, and the culture medium is obviously superior to a traditional coniferous tree sawdust culture medium and a broad-leaved tree sawdust culture medium. The culture medium for ganoderma sinense cultivation is easy to obtain, simple and convenient in preparation process, capable of effectively increasing the yield of ganoderma sinense and the content of main active ingredients and suitable for large-scale cultivation and application.