14 results about "Ganoderma sinense" patented technology

The present invention provides triterpenoid compounds in Ganoderma sinense fermented mycelia. The structural formulas of the triterpenoid compounds are Structural Formulas 1, 3, 4, and 7. The present invention also provides the application of the triterpenoid compounds in Ganoderma sinense fermented mycelia as a drug for preventing or treating inflammation; the triterpenoid compounds in the Ganoderma sinense mycelia are one or more of the compounds with Structural Formulas 1-8. The present invention for the first time conducts an anti-inflammatory activity test on the triterpenoid compounds isolated and purified from Ganoderma sinense mycelia, and finds that the compounds with Structural Formulas 1-8 can all inhibit the release of TNF-α and IL-6, and have significant anti-inflammatory activity.

The invention discloses a Ganoderma sinense flash-soluble tablet and a preparation method thereof, and belongs to the technical field of food health care, Ganoderma sinense sporocarp is subjected to deproteinization treatment to prevent precipitation generated by combination of tannic acid and Ganoderma sinense protein, and polysaccharide and triterpenes in Ganoderma sinense sporocarp are effectively retained and enriched after Ganoderma sinense sporocarp superfine powder is subjected to deproteinization treatment. In the preparation process, tannic acid serves as a structure trigger and is self-assembled with ganoderma sinense polysaccharide in deproteinized ganoderma sinense powder through hydrogen bonds to form a pH responsive hydration film, phenolic hydroxyl groups of tannic acid and alcoholic hydroxyl groups of beta-glucan form a multiple hydrogen bond network, aromatic rings are embedded into hydrophobic cavities of beta-glucan, and the compound is stabilized through the hydrophobic effect. By adopting hydroxypropyl-beta-cyclodextrin inclusion, the tannic acid is prevented from being combined with the ganoderma sinense sporocarp protein to form a precipitate in space. The Ganoderma sinensis flash-soluble tablet prepared by the invention is rich in triterpenes and polysaccharide components, can significantly improve immunity and regulate intestinal flora, and is suitable for health food development.

This invention relates to the field of traditional Chinese medicine component research technology, specifically to a method for constructing fingerprint spectra based on the antioxidant active components of Ganoderma lucidum and its application. First, a method for ultrasonic-assisted acid hydrolysis of crude Ganoderma lucidum polysaccharides guided by free radical scavenging activity was constructed. This method is efficient, easy to operate, and can effectively reflect the free radical scavenging activity of the depolymerized Ganoderma lucidum polysaccharides. Hydrophilic chromatography-electrospray ionization detector-electrospray mass spectrometry was used to acid hydrolyze and analyze 52 batches of Ganoderma lucidum polysaccharides from different sources. Further, combined with grey relational analysis and partial least squares regression analysis, 12 common activity peaks were screened, establishing fingerprint spectra based on the antioxidant active components of Ganoderma lucidum. Finally, principal component analysis and partial least squares discriminant analysis were used to analyze the fingerprint spectra based on the antioxidant active components of Ganoderma lucidum for standard Ganoderma lucidum, Ganoderma sinense, Ganoderma applanatum, Ganoderma lucidum var. truncatum, and sample Ganoderma lucidum, respectively, achieving the identification of Ganoderma lucidum varieties.

The invention discloses oral liquid with the effects of coordinating intestines and stomach, reducing blood fat and dissolving thrombus and a preparation method of the oral liquid, and belongs to the technical field of homology of medicine and food. The oral liquid is prepared by matching 15 natural traditional Chinese medicinal materials such as ganoderma sinense and American ginseng according to a specific proportion, strictly screening raw materials, decocting for three times to extract active ingredients, filtering and blending, adding acetic bacteria, lactic acid bacteria and saccharomycetes, and performing enzymolysis, metabolic transformation and symbiotic synergy on microorganisms to realize accurate transformation of traditional Chinese medicinal ingredients. And fermenting and extracting at 28-35 DEG C for six months. In the preparation process, the rhizome medicinal materials are pretreated, the blending proportion of the white granulated sugar is accurately controlled, and the quality is guaranteed through a strict quality inspection process. According to the oral liquid, natural components are used for replacing chemical medicines, multiple effects are integrated, and adverse reactions are avoided; the scientific preparation process ensures that the effective components are fully dissolved out, the bioavailability is remarkably improved, the product quality is stable and reliable, and a new solution is provided for related health problems.

The invention relates to the technical field of medicine preparation, and provides a probiotic ganoderma lucidum yeast tablet which comprises 30-50% of ganoderma sinense extract; 20%-35% of yeast powder; 10%-25% of microcrystalline cellulose; 1%-3% of magnesium stearate; the content of polysaccharide in the ganoderma sinense extract is greater than or equal to 15%, the content of triterpenoids in the ganoderma sinense extract is greater than or equal to 8%, and the flavoring agent is selected from one or a combination of more of menthol, stevioside and citric acid. The probiotic ganoderma lucidum yeast tablet is rich in high-content ganoderma sinense extract and yeast powder, in the ganoderma sinense extract, polysaccharide regulates the immune function, enhances the body resistance and assists in gastric mucosa repair, triterpenoids have anti-inflammatory and antioxidant activity and effectively relieve symptoms of patients, the yeast powder can promote food digestion and absorption and improve the gastrointestinal function, and the health care effect is achieved. The main components of the product are all natural, the safety is high, and the toxic and side effects are greatly reduced compared with traditional chemical synthesis drugs.

The invention relates to the technical field of microorganism culture, in particular to a ganoderma sinensis spore germination culture medium and application thereof.Ganoderma sinensis spores are collected through a fungus carrying bag or the culture medium, the optimal growth environment for ganoderma sinensis spore germination culture is obtained by optimizing the culture condition of the ganoderma sinensis spores, and the ganoderma sinensis spore germination culture medium is obtained by optimizing the culture medium formula and adding wood chip boiling liquid. A culture medium formula most suitable for ganoderma spore germination is obtained through raw material screening and orthogonal experiment optimization of boiling liquid, ganoderma spores can be effectively collected without reaching a sterile environment of a laboratory, the method is a practical and effective ganoderma sinensis spore collection and germination method, and a foundation is laid for promoting ganoderma sinensis new variety breeding research.

This invention discloses a polysaccharide from *Ganoderma lucidum* (also known as *Ganoderma lucidum* or *Ganoderma sinense*) with efficacy in alleviating colorectal cancer, its preparation method, and its applications, belonging to the field of biomedical technology. The polysaccharide is composed of mannose, glucose, galactose, and fucose, with a molar ratio of 3.221:1.414:80.539:14.826 and a molecular weight of 7.056 kDa. It has been confirmed that the polysaccharide can significantly reduce the number and size of colon tumors; alleviate pathological symptoms such as colonic weight index, bleeding, crypt budding, dysplasia, and hyperplasia; and significantly inhibit the levels of IFN-γ, TGF-β, and IL-17A in the colon while increasing the level of IL-4. Therefore, the polysaccharide provided by this invention has a significant effect in alleviating colorectal cancer, and this invention provides theoretical support for the development of effective and biosafe colorectal cancer drugs.

The present invention provides a method for simultaneously detecting 12 triterpenoid compounds in Ganoderma sinense mycelium, and the method comprises the following steps: (1) a pretreatment step of Ganoderma sinense mycelium; (2) an ultra-high performance liquid chromatography detection step; (3) a step of obtaining the mass spectrometry information of the compounds; (4) a step of establishing an ultra-high performance liquid chromatography-triple quadrupole mass spectrometry combined analysis method; (5) a data analysis step. The method for simultaneously detecting 12 triterpenoid compounds in Ganoderma sinense mycelium provided by the present invention can simultaneously perform accurate qualitative and quantitative analysis on 12 triterpenoid compounds metabolized in Ganoderma sinense fermented mycelium, has high sensitivity and strong specificity, and thus provides a rapid and accurate detection and analysis method for the research of triterpenoid compounds in Ganoderma sinense mycelium and the development of related products.

The application discloses a Ganoderma sinense triterpene extract, a preparation method thereof and anti-aging application. The application adopts liquid static fermentation to obtain Ganoderma sinense mycelium, extracts the mycelium after freeze-drying of the mycelium with an organic solvent, adds activated carbon for oscillation decolorization, further concentrates, adds water, and dries the precipitate part to obtain a C-3 β triterpene extract rich in multiple C-3 The extract has excellent anti-aging activity, can be added as a functional ingredient raw material in cosmetics, and has a broad market prospect.

The invention provides a method for simultaneously detecting 13 triterpenoids in ganoderma sinense sporocarp. The method comprises the following steps: (1) pretreating ganoderma sinense sporocarp; (2) an ultra-high performance liquid chromatography detection step; (3) obtaining mass spectrum information of the compound; (4) establishing a dynamic multi-reaction monitoring DMRM method; and (5) a data analysis step. According to the method for simultaneously detecting the 13 triterpenoids in the ganoderma sinense sporocarp, provided by the invention, quantitative analysis of the 13 characteristic triterpenoids in the ganoderma sinense sporocarp is realized, and an accurate and reliable detection means is provided for related research and development of the ganoderma sinense sporocarp.

The invention provides a formula of ganoderma lucidum wine and a preparation method thereof, and relates to the field of health wine, and the ganoderma lucidum wine is characterized by comprising the following raw materials by weight: 3 g of white peony root, 5 g of safflower, 10 g of rheum officinale, 8 g of radix rehmanniae, 1 g of radix aconiti agrestis, 5 g of clove, 1 g of rhizoma nardostachyos, 7 jin of ganoderma lucidum, and 2000 jin of wine body. The preparation method comprises the following steps: step 1, mixing radix paeoniae alba, safflower, rheum officinale, radix rehmanniae, radix aconiti agrestis, clove and rhizoma nardostachyos, and performing pretreatment; step 2, pretreating the ganoderma lucidum; the method has the advantages that by optimizing the extraction process, the extraction rate of active polysaccharides, triterpenoids, amino acids and other functional components in ganoderma lucidum is remarkably increased, meanwhile, activity loss caused by long-time high temperature or oxidation in a traditional soaking method is avoided, and the method can effectively retain the synergistic biological activity of ganoderma lucidum and ganoderma lucidum and has a good application prospect. The health-preserving effects of immunoregulation, oxidation resistance and the like of the product are enhanced.

The invention discloses a preparation method of ganoderma lucidum tea. The preparation method comprises the following steps: (1) pretreating ganoderma lucidum: cutting the ganoderma lucidum into fine particles which are uniform in size and have the particle size of not more than 3mm; (2) stir-frying the ganoderma lucidum granules, wherein the temperature is controlled to be 120-150 DEG C, and the stir-frying time is 5-10 minutes; the invention further provides the ganoderma lucidum tea prepared by the method, and the ganoderma lucidum tea can be independently brewed in water for drinking and can also be compounded with ganoderma sinense or other tea leaves for brewing in water for drinking. According to the ganoderma lucidum tea provided by the invention, the bitter taste of ganoderma lucidum is effectively removed, the effective components of ganoderma lucidum are reserved, and the processing technology brings a brand new product form and a higher quality standard for the ganoderma lucidum tea market.

The invention discloses a pharmaceutical composition for clearing lung heat and eliminating stagnation and treating lung cancer and a preparation method thereof, and relates to the technical field of traditional Chinese medicine, the pharmaceutical composition is composed of pinellia ternate, ganoderma sinense, phellinus igniarius, coriolus versicolor, semen lepidii, nightshade, scorpio, pinellia cordata, astilbe chinensis, oldenlandia diffusa, radix ranunculi ternati, pseudo-ginseng powder, radix semiaquilegiae, apios fortunei maxim, thunberg fritillary bulb and bulbus fritillariae cirrhosae. The pharmaceutical composition has the advantages of high purity and stability of medicinal components, and in the actual use process, the pharmaceutical composition is composed of pinellia ternate, ganoderma sinensis, phellinus igniarius, coriolus versicolor, semen lepidii, black nightshade, scorpio, pinellia cordata, astilbe chinensis, oldenlandia diffusa, radix ranunculi ternati, pseudo-ginseng powder, radix semiaquilegiae, apios fortunei maxim, thunberg fritillary bulb, bulbus fritillariae cirrhosae and other traditional Chinese medicinal materials which can achieve a synergistic effect and have a synergistic effect. Excellent multiple pharmacological actions such as tumor resistance, inflammation resistance, oxidation resistance and the like are shown; specifically, the lung-clearing and stagnation-eliminating formula effectively promotes discharge of turbid phlegm in the lung and relieves the cough symptom through a unique pharmacodynamic mechanism of the lung-clearing and stagnation-eliminating formula.

The invention provides a Ganoderma sinense strain LY3 and an extract and application thereof, and belongs to the technical field of edible and medicinal fungus strain resources and functional product development, the Ganoderma sinense strain LY3 has the preservation number of CGMCC No.42570, and the Ganoderma sinense strain LY3 has the preservation number of CGMCC No.42570. The ITS sequence is as shown in SEQ ID NO. 1. The ganoderma sinense strain LY3 has the characteristics of high polysaccharide and high triterpenes, the extract of the ganoderma sinense strain LY3 has a remarkable effect on an anti-aging model, and the LY3 extract can remarkably reduce the malondialdehyde and active oxygen level in caenorhabditis elegans; meanwhile, the expression of antioxidant enzymes such as total superoxide dismutase, glutathione peroxidase and catalase is improved, and an obvious dose-effect relationship is shown; the ganoderma sinensis strain LY3 disclosed by the invention can be used for stable and industrialized anti-aging product development.