3 results about "Ganoderma sinense" patented technology

This invention relates to the field of traditional Chinese medicine component research technology, specifically to a method for constructing fingerprint spectra based on the antioxidant active components of Ganoderma lucidum and its application. First, a method for ultrasonic-assisted acid hydrolysis of crude Ganoderma lucidum polysaccharides guided by free radical scavenging activity was constructed. This method is efficient, easy to operate, and can effectively reflect the free radical scavenging activity of the depolymerized Ganoderma lucidum polysaccharides. Hydrophilic chromatography-electrospray ionization detector-electrospray mass spectrometry was used to acid hydrolyze and analyze 52 batches of Ganoderma lucidum polysaccharides from different sources. Further, combined with grey relational analysis and partial least squares regression analysis, 12 common activity peaks were screened, establishing fingerprint spectra based on the antioxidant active components of Ganoderma lucidum. Finally, principal component analysis and partial least squares discriminant analysis were used to analyze the fingerprint spectra based on the antioxidant active components of Ganoderma lucidum for standard Ganoderma lucidum, Ganoderma sinense, Ganoderma applanatum, Ganoderma lucidum var. truncatum, and sample Ganoderma lucidum, respectively, achieving the identification of Ganoderma lucidum varieties.

The application discloses a Ganoderma sinense triterpene extract, a preparation method thereof and anti-aging application. The application adopts liquid static fermentation to obtain Ganoderma sinense mycelium, extracts the mycelium after freeze-drying of the mycelium with an organic solvent, adds activated carbon for oscillation decolorization, further concentrates, adds water, and dries the precipitate part to obtain a C-3 β triterpene extract rich in multiple C-3 The extract has excellent anti-aging activity, can be added as a functional ingredient raw material in cosmetics, and has a broad market prospect.

The invention provides a Ganoderma sinense strain LY3 and an extract and application thereof, and belongs to the technical field of edible and medicinal fungus strain resources and functional product development, the Ganoderma sinense strain LY3 has the preservation number of CGMCC No.42570, and the Ganoderma sinense strain LY3 has the preservation number of CGMCC No.42570. The ITS sequence is as shown in SEQ ID NO. 1. The ganoderma sinense strain LY3 has the characteristics of high polysaccharide and high triterpenes, the extract of the ganoderma sinense strain LY3 has a remarkable effect on an anti-aging model, and the LY3 extract can remarkably reduce the malondialdehyde and active oxygen level in caenorhabditis elegans; meanwhile, the expression of antioxidant enzymes such as total superoxide dismutase, glutathione peroxidase and catalase is improved, and an obvious dose-effect relationship is shown; the ganoderma sinensis strain LY3 disclosed by the invention can be used for stable and industrialized anti-aging product development.