42 results about "Cephalotaxus" patented technology

The invention provides a quick and high-efficiency cephalotaxus hainanensis seed germinating and high-quality seedling breeding method which relates to a quick and high-efficiency plant seed germinating and high-quality seedling breeding method. The method comprises the following steps: (1) seed collection: collecting cephalotaxus hainanensis seeds to be matured or matured seeds; (2) preparation of seed germinating matrixes; (3) release of seed dormancy; (4) seed germination; and (5) acclimatization and transplantation. The invention can enhance the seed germinating bud ratio by more than 85%,the seedling viability exceeds 90%, the uniform high-quality cephalotaxus hainanensi seedlings can be produced, and the viability of seeds transplanted to fields can also exceed 90%; compared with the traditional method, the stocking time is shortened by about 2 / 3; the related facilities and processes of tissue culture and the like are not required, so the operation is convenient; the invention provides technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and the utilization of resources.

The invention provides a quick and high-efficiency cephalotaxus hainanensis seed seedling breeding method which belongs to a quick and high-efficiency plant seed seedling breeding method. The method comprises the following steps: (1) seed collection; (2) cleaning and sterilization of seeds; (3) release of seed dormancy at low temperature; (4) sterile germination of seeds; and (5) acclimatization and transplantation. The invention can enhance the seed germinating bud rate to more than 90% (enhanced by about 70%), and the seeding viability exceeds 90% (enhanced by about 60%); compared with the traditional method, the stocking time is shortened by about 2 / 3; the regular high-quality cephalotaxus breeding seedlings can be produced; and the invention provides technical support for the quick propagation and restoration protection of rare or endangered species of cephalotaxus hainanensis and the development and utilization of resources.

The invention relates to a plant cell culture technology, in particular to a method for biosynthesizing abietane diterpenoid compounds by using cephalotaxus fortune culture cells. The method comprises the following steps of: placing cephalotaxus fortune cells in a common culture medium, and culturing, wherein50 to 150g of the cells are inoculated in each liter of wet weight of the culture medium; and adding an inducer at the final concentration of 10 to 2,000 mu M and 50 to 200g of adsorbent into each liter of culture medium at the initial stage of cell exponential growth, wherein the inducer is an abiological inducer or fungal elicitor. The method is a biosynthesis method and is stable and environment-friendly; and products are completely adsorbed onto the adsorbent, so that the operation process of separation and purification is greatly simplified, the cost is reduced and industrial production is easy to realize. Meanwhile, seven abietane diterpenoid compounds comprising a new compound are synthesized. The abietane diterpenoid compounds have high bioactivity, and are important lead compounds, and the technology has important commercial application prospect.

An application of Chinese cephalotaxus in preparing herbicide includes extracting the herbiciding component in organic solvent, separating and preparing herbicide. Its advantage is high herbiciding activity to weeds including wild oats, barnyard grass, etc.

The invention discloses an organic ecological pesticide formulation and a preparation method. The organic ecological pesticide formulation comprises the following raw materials by weight percent: 5-10% of maidenhair, 8-10% of tropic ageratum herb, 2-10% of cephalotaxus fortune, 5-10% of asparagus, 3-10% of rotenone, 15-10% of ethyl alcohol, 10-10% of tobacco stem, 2-10% of gelsmium elegans, 2-10% of strophanthus divaricatus, 2-10% of castor beans shell, 2-10% of derris, 4-10% of neem leaves, 10-30% of pyrethrum, 10-15% of sweet wormwood, 5-10% of garlic, 10-30% of chili, 5-10% of fresh ginger and 2-10% of root bark of the peony tree. Compared with the prior art, the invention has the advantages that the organic ecological pesticide formulation is formed by natural organic biological fermentation, no chemical is added, the insecticidal efficacy is excellent, no toxic drug is left, the pesticide can be absorbed by plants, the pesticide can be prepared into a nutrient solution having a pesticidal function, the use is convenient and the pesticide has popularization and application values.

The invention relates to a separation and purification method of optically pure levorotatory cephalotaxine. The levorotatory optically pure cephalotaxine is colorless transparent acicular crystal, the optical purity is larger than 99 percent, the specific rotatory power [alpha]D is equal to -194 degrees[c 1.02, CHCl3, 20 DEG C], and the melting point is 143-144 DEG C. Cephalotaxus fortunei biological total alkali extract extracted from cephalotaxus plants or residual extract after cephalotaxus ester base is extracted from the cephalotaxus plants is used as raw material, mixed solvent of water and dichloromethane is used for dissolving the raw material, and the dichloromethane is firstly used for the extraction under acid condition; separated water phases are successively extracted by ether under alkali condition and methylene dichloride under acid condition; the water phases are extracted by the ether under the alkali condition, ether phases are condensed and crystallized to obtain the target product. The method has the advantages of simple operation, safety, reliability, high efficiency, high yield and low cost and can easily realize the scale preparation of the optically pure levorotatory cephalotaxine.

The invention discloses a hydrogen peroxide / polysilicate aluminum sulfate compounded blue algae treating agent which is characterized by being prepared from the following raw materials in parts by weight: 26-29 parts of polysilicate aluminum sulfate, 18-20 parts of aluminum potassium sulfate, 13-15 parts of H2O2, 16-18 parts of lignosulfonate, 14-17 parts of ammonium persulfate, 19-20 parts of potassium sorbate powder, 17-19 parts of saw powder, 16-20 parts of radix cephalotaxus fortune, 17-21 parts of corncobs, 10-13 parts of eucalyptus leaves, 1-3 parts of coptis chinensis and 2-5 parts of golden cypress. The blue algae treating agent prepared by adopting the method disclosed by the invention is rapid in sewage treatment, and can be used for effectively removing seed sources of overwintering water-blooming cyanobacteria in a large shallow lake as well as treating and managing cyanobacterial bloom, so that a new way for preventing and controlling the occurrence of harmful algal bloom is provided.

The invention provides a method for culturing cephalotaxus excised embryos and regenerating plants, which belongs to the cephalotaxus culture technology. The method aims to solve the problem that the prior art is difficult to get ideal germination rate and more robust seedlings because of the long dormancy period of cephalotaxus seeds, long emergence time and low emergence rate. The method is characterized in that fresh plump seeds are selected as materials and subjected to induction culture; robust sprouts can be germinated from embryos after 15 to 20 days; seedlings can be formed and transplanted after 20 to 25 days of proliferation and 25 to 30 days of root induction; and the survival rate is as high as above 90 percent. The method has the advantage of high propagation factor, and surviving seedlings are good in growth, robust and upright.

The invention relates to a method for extracting cephalotaxine from cephalotaxus fortunei. The method comprises the following treatment steps of: a, pulverizing branches and leaves of cephalotaxus fortune and sieving with a 100-mesh sieve; b, carrying out ultrasonic-microwave-assisted extraction in an acidic ethanol solution; c, adjusting the pH value of the solution with an alkali and purifying and separating the solution by virtue of an aluminum trioxide column; d, extracting with chloroform for 2-3 times and carrying out reduced pressure concentration on the extract to obtain a crude product of cephalotaxine; and e, re-crystallizing the crude product of cephalotaxine with anhydrous ethanol twice to obtain a pure cephalotaxine. According to the method disclosed by the invention, the cephalotaxine is extracted by virtue of the modern extraction technology, namely, combined ultrasonic-microwave-assisted extraction method and the method has the advantages of simple process, high efficiency and low cost and the high-purity cephalotaxine is easily formed in an industrial production.

The invention discloses a method for extracting cephalotaxine from cephalotaxus hainanensis, after an extraction solvent is added, cephalotaxus hainanensis powder is extracted by an ultrasonic extraction device to obtain a first extracting solution, and a sulfuric acid aqueous solution or a methanol solution is used as the extraction solvent, so that economy and environmental friendliness can be realized, and industrial production can be realized; the first extracting solution is centrifuged, then supernate is poured out, the operations of adding the extracting solvent, carrying out ultrasonic extraction, centrifuging and pouring out the supernate after centrifuging are repeatedly executed for 1-4 times, the supernate is combined to obtain a second extracting solution, and the second extracting solution is successfully separated and purified to obtain a cephalotaxine monomer by adopting a method for separating and purifying cephalotaxine through preparative high performance liquid chromatography. The extraction quality of the cephalotaxine can be improved through multiple extraction, the efficiency is high, and the technical problems that in the prior art, the cephalotaxine is extracted from cephalotaxus plants through organic solvents such as ethyl alcohol, the cost is high, industrial production is not facilitated, column chromatography is mostly adopted during separation and purification, the solvent dosage is large, and the efficiency is low are solved.

The invention belongs to the field of plant endophyte separation and microbial fermentation engineering, and in particular relates to a method for preparing a crude torreya alkaloid product. By selecting the roots, stems, and leaves of perennial Torreya as explants, cutting them into strips, sterilizing them, and putting them into the culture medium to cultivate them to grow the symbiotic bacteria of Torreya, and then isolate the metabolizable Torreya symbiotic bacteria from the symbiotic bacteria. Torreya alkaloid strains are cultivated and fermented, and the product is obtained by concentrating the fermented liquid and separating out. In the present invention, in the preparation process of the fermentation liquid, the second batch of strains is inserted after the strains produce metabolic organisms, and the metabolites produced by the previous batch of strains are used to provide nutrients for the second batch of strains, which greatly promotes the production of the second batch of strains. The growth of the bacteria species improves its biological activity, thereby producing a large amount of fermentation liquid and improving the output of crude torreya alkaloids.

The invention discloses an anticancer aroma-enhancing filter composite for smoking, comprising the following substances in parts by mass: 2-3 parts of taxus, 0.5-1 part of zedoary, 0.5-1 part of jobs tears, 0.5-1 part of catharanthus roseus, 0.5-1 part of cephalotaxus fortune, 0.5-1 part of dysosma versipellis, 0.5-1 part of rabdosia rubescens, 0.5-1 part of meadow saffron, 0.5-1 part of camptotheca acuminata decne and 0.5-1 part of brucea javanica. Through the anticancer aroma-enhancing filter composite for smoking, provided by the invention, a lot of carcinogenic substances such as nicotine, neonicotine and the like in the smog generated by tobacco combustion can be reacted and neutralized; after a small quantity of carcinogenic substances are inhaled, as a lot of anticancer substances and substances for strengthening the immunity are inhaled, the harm to the human organs, caused by the carcinogenic substances, are greatly decreased, and the harm to the human body, caused by the smog, is minimized; and the original flavor of the tobacco is not destroyed by simply and largely adsorbing the smog, and meanwhile, a lot of plant spices are added, so that the anticancer aroma-enhancing filter composite for smoking has purer taste and can be accepted more easily.

The invention discloses a traditional Chinese medicine composition for treating leukemia. The composition comprises the following raw medicines by weight: 3-10 grams of cephalotaxus fortune, 3-10 grams of camptotheca acuminate, 3-10 grams of catharanthus roseus, 9-20 grams of indigo naturalis, 9-20 grams of folium isatidis, 3-10 grams of diversifolious patrinia root, 5-20 grams of common threewingnut root, 3-10 grams of galium aparine, 5-20 grams of calculus bovis, 3-10 grams of vietnamese sophora root, 9-20 grams of lithospermum and 9-20 grams of rumex japonicus root. The composition has the beneficial effects that the indigo naturalis and the calculus bovis mainly have the effects of clearing heat and removing toxicity; the medicines are combined and jointly have the effects of benefiting qi and nourishing blood, removing toxicity and clearing marrow, cooling blood and activating blood to promote differentiation and apoptosis of juvenile cells. The composition is used for treating the symptoms such as fever, heat, headache, bone pain, anemia, hemorrhage, palpitation, lack of strength, tinnitus, soreness and weakness of waists and knees, lumps in the abdomen, scrofula, subcutaneous nodule, petechiae on the tongue, greasy fur tongue, slippery pulse or wiry pulse and the like caused by various acute and chronic leukemia with internal toxicity stasis and qi-blood deficiency.

The invention relates to a traditional Chinese medicine composition and a preparation method thereof. The traditional Chinese medicine composition is mainly prepared from the following components: gastrodia elata, tendril-leaved fritillary bulb, thunberg fritillary bulb, ginseng, salviae miltiorrhizae, radix scrophulariae, radix sophorae flavescentis, the roots of straight ladybell, radix stemonae, ligusticum wallichii, red peony roots, angelica sinensis, dried radix rehmanniae, radix rehmanniae preparata, saffron crocus, peach kernels, radix ophiopogonis, schisandra chinensis, American ginseng, astragalus, kudzuvine roots, leeches, scorpio, stiff silkworms, earthworms, rhizoma acori graminei, irkutsk anemone rhizome, apricots, bunge cherry seeds, erodium stephanianum willd, uncaria, semen lepidii, penthorum chinense pursh, poria cocos, poria with hostwood, martin fig roots and stems, radix aconiti preparata, rhizome arisaematis, arisaema cum bile, semen boitae, turtle shells, manis pentadactyla, tortoise shells, zaocys dhumnade, agilawood, kadsura coccinea, centipedes, rhizoma sparganii, curcuma zedoary, ground beetles, cephalotaxus fortune, rhizoma curculiginis, hairyvein agrimony, herba epimedii and the like. The preparation method is simple in process, and the prepared traditional Chinese medicine composition is convenient to take and has obvious effects for treating serious diseases such as hydrops and tumors of the lung and heart and cerebral vessels.

The invention relates to cephalotaxus fortunei norditerpenoid compounds expressed as formula (I), pharmacologically acceptable salts thereof, a pharmaceutical composition taking the cephalotaxus fortunei norditerpenoid compounds as active components and application of the cephalotaxus fortunei norditerpenoid compounds to preparation of anti-tumor medicines. A series of novel cephalotaxus fortunei norditerpenoid compounds are discovered in cephalotaxus plants; extracorporeal activity screening shows that the compounds has significant cytotoxic activity and anti-inflammatory activity; mechanism research shows that the cephalotaxus fortunei norditerpenoid compounds are different from colchicines containing cycloheptenone, have no influence on microtubule polymerization and are relevant with inhibition of NF-kB signal channels; in-vivo transplantation tumor activity evaluation shows that the cephalotaxus fortunei norditerpenoid compounds have significant anti-tumor activity and have good pharmacy prospects.

The invention discloses a preparation method of a natural solid granular insecticide and belongs to the technical field of pesticide. In allusion to the problem that currently traditional insecticides are mostly chemical preparations, have high toxicity, are easy to harm safety of human and livestock and severely affect environmental health and safety, the invention provides a natural solid granular insecticide which is prepared by steps of using modified and carbonized cephalotaxus fortune branch particles as a carrier, mixing crotalaria mucronata root, maidenhair and the cephalotaxus fortune branch particles and fermenting, filtering and the like. The prepared insecticide is a natural insecticide, causes no pollution to the environment, contains no harmful substances, and is harmless to human body. Insecticidal rate of the insecticide reaches 96% and above.

The invention discloses a parasite expelling care solution for pets. The parasite expelling care solution for the pets is prepared from, by mass, 12-14 parts of goldencup hypericum, 15-20 parts of hemp fimble stem-fibre, 15-18 parts of white poplars, 8-10 parts of coralline algae, 5-8 parts of grateloupia livida, 2-4 parts of gelidium amanisi, 6-8 parts of dendrocnide urentissima, 13-16 parts of dendrobenthamiajaponi-cavar.chinensis(Osborn)Fang., 20-25 parts of ishige okamurae, 22-24 parts of milingtonia hortensis L., 30-35 parts of cephalotaxus sinesis, 16-18 parts of dendrobenthamiahongkongensis(Hemsh.)Hutch., 5-10 parts of atremisia frigida, 9-14 parts of rubus delavayi Franch., 15-18 parts of fraxinus ferruginea lingelsh, 5-10 parts of argy wormwood leaves, 22-24 parts of radix stemonae and 13-18 parts of ailanthus altissima. The parasite expelling care solution for the pets can treat enteritis caused by parasites, relieve symptoms of constipation or diarrhea of the pets, side effects do not exist, and the effect is obvious.

The invention belongs to the field of plant endophyte separation and microorganism fermentation engineering, and in particular relates to a preparation method of a cephalotaxus alkaloid product. The preparation method comprises the following steps: by adopting roots, stems and leaves of perennial cephalotaxus as explants, cutting into strips, sterilizing, then culturing in a culture medium to grow cephalotaxus symbiotic bacteria, then separating strains capable of metabolizing cephalotaxus alkaloid from the cephalotaxus symbiotic bacteria and performing culture and fermentation, concentrating a fermentation liquid, and then precipitating to prepare a product. According to the preparation method disclosed by the invention, in a preparation process of the fermentation liquid, a second batch of strains are inoculated after the strains generate metabolic organisms, metabolic products of the former batch of the strains are used for providing nutrient substances for the second batch of the strains, so that the growth of the second batch of the strains can be greatly promoted, and the biological activity of the strains also can be improved, and thus a great number of fermentation liquids can be generated, and the yield of cephalotaxus alkaloid can be improved.

The invention belongs to the field of plant endophyte separation and microorganism fermentation engineering, and in particular relates to an industrial production method of cephalotaxus alkaloid. The industrial production method comprises the following steps: by adopting roots, stems and leaves of perennial cephalotaxus as explants, cutting into strips, sterilizing, then culturing in a culture medium to grow cephalotaxus symbiotic bacteria, then separating the cephalotaxus symbiotic bacteria according to different strains and respectively performing culture and fermentation, concentrating a fermentation liquid and screening to obtain the strains capable of metabolizing cephalotaxus alkaloid, performing fermentation and culture, separating the fermentation liquid from a culture product, and performing alcohol degradation, suction filtration and drying to prepare cephalotaxus alkaloid. According to the industrial production method disclosed by the invention, activated strains are firstly inoculated into a shake flask filled with a fermentation culture medium for culturing, and then are subjected to amplification culture by virtue of a four-stage seeding tank to ensure that the strains are stable in physiological state and strong in activity, and can rapidly grow after transplanting; and a great number of high-quality strains can be obtained in a short time, the fermentation time can be shortened, and the production efficiency can be improved.

The present invention provides novel cephalotaxus esters, syntheses thereof, and intermediates thereto. The invention also provides pharmaceutical compositions comprising a compound of the present invention and methods of using said compounds or compositions in the treatment of proliferative diseases (e.g., benign neoplasm, cancer, inflammatory disease, autoimmune disease, diabetic retinopathy) and infectious disease. The invention further provides methods of using said compounds or compositions in the treatment of multidrug resistant cancer.

The invention discloses a preparation process for homoharringtonine. The preparation process comprises the following steps: selecting a raw material, namely branches of cephalotaxus fortunei, thoroughly removing impurities, crushing the branches, performing extracting with methanol, combining liquid extracts, and carrying out concentrating under reduced pressure to obtain an extract 1; and subjecting the extract 1 to stirring and dissolving, adjusting a pH value, carrying out standing and centrifugation, adding petroleum ether, and carrying out extraction on acid precipitation supernatant multiple times. According to the invention, the content of homoharringtonine in a finally obtained sample is larger than 99%, so yield and purity are greatly improved compared with a traditional technology; only branches and leaves of cephalotaxus chinensis are used as raw materials for preparing homoharringtonine in the preparation method, so industrial production can be carried out on the premise that normal growth of tree species is not affected and the environment is not damaged; used reagents, and namely methanol and chloroform can be effectively recovered and repeatedly utilized in the preparation process, so environmental pollution is reduced to the maximum extent while an actual utilization rate is increased.

The invention relates to the technical field of agriculture cutting seedling, in particular to a cephalotaxus fortune cutting seedling rooting-promoting compound conditioner and preparation method thereof. The cephalotaxus fortune cutting seedling rooting-promoting compound conditioner is composed of the following components in weight: 600-850 mg of rooting conditioner, 10-30 mg of rooting additive, 10-25 mg of nutrient, 2-5 g of bactericide, and the balance of water and ethanol. The preparation method comprises the following steps: dissolving the rooting conditioner and the rooting additive into an I solution by using 95% ethanol, dissolving the nutrient and the bactericide into an II solution by using water; mixing I and II, adding water till a metered volume of 1000mL, uniformly stirring to obtained a finished solution for later use; through reasonable proportion of raw material components, effectively activating cambial cells to form callus at a cutting notch within short time, inducing the morphogenesis of adventives root, and improving the cutting rooting percentage and the cottage survival rate, wherein the average rooting rate of the twig cutting seedling achieves 99%, and the production cost is reduced.

The invention discloses a preparation method of a nightshade anti-cancer shampoo and belongs to the technical field of cleaning solutions. The preparation method comprises the following steps: A, keeping the temperature at 70-72 DEG C, adding water into a stirrer, then adding sodium lauryl polyoxyethylene ether sulfate, sodium chloride, coconut oil fatty acid monoethanolamide, cetearyl alcohol, polyquaternium-100 and ethylene diamine tetraacetic acid tetrasodium salt, fully stirring, and standing for defoaming; B, adding nightshade, ginseng, cleavers, cephalotaxus fortune and Isodon excisus(Maxim.)Kudo into a mixture obtained in the step A, and grinding; C, keeping the temperature at 40-46 DEG C, adding an essence and glycerinum into the stirrer used in the step A, and fully stirring to obtain a mixed solution; D, adjusting the pH value of the mixed solution obtained in the step C to be 5.5-6.5; E, standing for defoaming, and sub-packaging after qualified detection. The prepared nightshade anti-cancer shampoo can nourish and smoothing hair, repair dry, frizzy and sallow hair and can play an anti-cancer role.

The invention provides a fodder and feeding method of anti-cancer health protection chicken. The feeding method comprises the following steps: In the first stage, the anti-cancer health protection chicken are fed for 25-35 days. The Chinese herbal medicine component of the fodder comprises, by weight, 2-8 parts of toad, 1-3 parts of cephalotaxus, 1-3 parts of edible tulip, 1-3 parts of paris polyphylla and 1-3 parts of gelsemium elegan. In the second stage, the anti-cancer health protection chickens are fed for 25-35 days. The Chinese herbal medicine component of the fodder comprises, by weight, 2-4 parts of hibiscus leaves, 2-4 parts of Centipeda minima, 1-3 parts of catharanthus roseus, 1-3 parts of bock greenbrier rhizome and 1-3 parts of semiaquilegia adoxoides. In the third stage, the anti-cancer health protection chickens are fed for 25-35 days and the Chinese herbal medicine of the fodder comprises, by weight, 2-8 parts of blackberry lily, 1-3 parts of cocklebur fruit, 1-3 parts of nightshade and 2-4 parts of mock-strawbery. The anti-cancer health protection chicken, fed by the fodder, has the advantages of preventing the spread of cancer cells effectively, reducing a degree of tumor and enhancing the immunity of human body.

The invention relates to the seedling raising technology in agriculture and forestry and in particular relates to a special compound fertilizer for raising seedlings of CephalotaxusfortuneiHook.f outdoors and an application method thereof. The special compound fertilizer comprises a water retaining and growth promoting rooting agent for transplanting and a special fertilizer for leaf surfaces, wherein the water retaining and growth promoting rooting agent for transplanting is prepared by mixing indolebutyric acid, 2,4-dichlorphenoxyacetic acid, humic acid, potassium nitrate, magnesium sulfate, calcium chloride, 6-BA, sodium polyacrylate and carbendazim; the special fertilizer for leaf surfaces is prepared by mixing compound amino acid, urea, monopotassium phosphate, zinc sulfate, compound sodium nitrophenolate, DA-6 and Tween. The water retaining and growth promoting rooting agent for transplanting and the special fertilizer for leaf surfaces are fully and effectively compounded according to the requirements of CephalotaxusfortuneiHook.f woods for nutrient elements, and then the requirements in the process of transplanting CephalotaxusfortuneiHook.f from greenhouses to outdoor seedling nurseries are met and nutrients are quickly provided for CephalotaxusfortuneiHook.f seedlings to enhance the adaptive capacity of CephalotaxusfortuneiHook.f, thus shortening the rejuvenation period and the nursery coming-out time, increasing the survival rates of the CephalotaxusfortuneiHook.f seedlings and reducing the cost of industrialized production.

The present invention relates to the field of pharmaceutical compounds and preparation methods thereof, and particularly relates to a Cephalotaxus ester alkaloid, and preparation method and use thereof. The Cephalotaxus ester alkaloid has a structure of formula (I). The present invention further provides a preparation method of the compound, comprising reacting a compound of formula (11) with a compound of formula (12) in the presence of a Lewis acid.

The invention discloses an anticancer aroma-enhancing filter composite for smoking, comprising the following substances in parts by mass: 2-3 parts of taxus, 0.5-1 part of zedoary, 0.5-1 part of jobs tears, 0.5-1 part of catharanthus roseus, 0.5-1 part of cephalotaxus fortune, 0.5-1 part of dysosma versipellis, 0.5-1 part of rabdosia rubescens, 0.5-1 part of meadow saffron, 0.5-1 part of camptotheca acuminata decne and 0.5-1 part of brucea javanica. Through the anticancer aroma-enhancing filter composite for smoking, provided by the invention, a lot of carcinogenic substances such as nicotine, neonicotine and the like in the smog generated by tobacco combustion can be reacted and neutralized; after a small quantity of carcinogenic substances are inhaled, as a lot of anticancer substances and substances for strengthening the immunity are inhaled, the harm to the human organs, caused by the carcinogenic substances, are greatly decreased, and the harm to the human body, caused by the smog, is minimized; and the original flavor of the tobacco is not destroyed by simply and largely adsorbing the smog, and meanwhile, a lot of plant spices are added, so that the anticancer aroma-enhancing filter composite for smoking has purer taste and can be accepted more easily.

The invention relates to a plant cell culture technology, in particular to a method for biosynthesizing abietane diterpenoid compounds by using cephalotaxus fortune culture cells. The method comprises the following steps of: placing cephalotaxus fortune cells in a common culture medium, and culturing, wherein50 to 150g of the cells are inoculated in each liter of wet weight of the culture medium; and adding an inducer at the final concentration of 10 to 2,000 mu M and 50 to 200g of adsorbent into each liter of culture medium at the initial stage of cell exponential growth, wherein the inducer is an abiological inducer or fungal elicitor. The method is a biosynthesis method and is stable and environment-friendly; and products are completely adsorbed onto the adsorbent, so that the operation process of separation and purification is greatly simplified, the cost is reduced and industrial production is easy to realize. Meanwhile, seven abietane diterpenoid compounds comprising a new compound are synthesized. The abietane diterpenoid compounds have high bioactivity, and are important lead compounds, and the technology has important commercial application prospect.