Application of seaweed polysaccharides

A technology of seaweed polysaccharide and kelp, which is applied in the field of biomedicine, can solve the problems that have not been reported, and achieve the effects of abundant raw material sources, low production cost, obvious antiviral effect and immune enhancement effect

Active Publication Date: 2015-08-05
INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Seaweed polysaccharides have been found to have a variety of biological activities. The activities that have been publicly reported include seaweed polysaccharides having antioxidant, antitumor, antibacterial, antiviral, and anticoagulant activities. However, for seaweed polysaccharides as immune enhancers, especially Its use in the prevention and treatment of avian influenza virus has not been reported

Method used

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  • Application of seaweed polysaccharides
  • Application of seaweed polysaccharides
  • Application of seaweed polysaccharides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Taking centipede algae, Ulva pore and Sargassum as examples to extract seaweed polysaccharides:

[0035] Take 100 g of crushed centipede algae (Grateloupia filicina), add 5000 g of distilled water, and extract in a water bath at 100° C. for 2 hours; use 100 mesh, 200 mesh, and 300 mesh sieves to filter out the filter residue, and then dialyze the filtrate to desalinate and then use reducing Concentrate to 1 / 10 of the original volume by pressing the concentrating equipment, precipitate with 4 times the volume of 95% ethanol for 24 hours, centrifuge, freeze-dry the precipitate, and obtain the centipede algal polysaccharide, which is ready for use.

[0036] Add 40 times of distilled water to the crushed Ulva Pertusa, and extract it in a water bath at 125°C for 4 hours; use 100 mesh, 200 mesh and 300 mesh sieves to filter out the filter residue, and then dialyze the filtrate to desalinate it with reducing Concentrate to 1 / 10 of the original volume by pressing the concentrat...

Embodiment 2

[0042] In vitro antiviral experiment

[0043] 1) Anti-H9N2 avian influenza virus

[0044] Madin-Darby canine kidney (MDCK) cells were grown into a monolayer on a 24-well cell culture plate in DMEM medium containing 10% fetal bovine serum, the culture medium was discarded, washed twice with PBS, and then inoculated with H9N2 virus. After one hour of adsorption, the virus was discarded, washed twice with PBS, and DMEM medium containing 100 ug / ml or 20 ug / ml of seaweed polysaccharide and 1% fetal bovine serum obtained from the above-mentioned embodiments was added, and the negative control group did not add polysaccharide. 37°C, 5% CO 2Incubate in the environment for 24h. The culture medium was taken to measure the hemagglutination titer (Hemagglutination test, HA), the cellular RNA was extracted after washing twice, and the expression level of H9N2 was detected by fluorescent quantitative PCR after reverse transcription. Experiments have shown that after adding the seaweed po...

Embodiment 3

[0052] Embodiment 3 animal immunity and humoral immunity

[0053] Polysaccharides can promote the proliferation and differentiation of B cells, leading to the production of antibodies. On this basis, 80 6-week-old Kunming mice were randomly divided into 8 groups, 10 in each group. The final concentrations of 10mg / kg and 50mg / kg of centipede algae, Ulva pore, Sargassum polysaccharide and H9N2 avian influenza inactivated virus were mixed and immunized twice by intraperitoneal inoculation, and two groups of control groups were set up at the same time. PBS and inactivated virus were injected separately, blood was collected 14 days after the two immunizations, and serum was separated. The effect of seaweed polysaccharides on the immune function of the body was evaluated by measuring the serum AIV specific antibody by enzyme-linked immunosorbent assay. Through this experiment, it can be found that the polysaccharide can significantly stimulate the production of specific antibodies...

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Abstract

The invention belongs to the technical field of biomedicines, and in particular relates to application of seaweed polysaccharides. The seaweed polysaccharides can be taken as preparations for preparing anti-viral or immune medicines. Cell models and in-vivo animal experiments show that the seaweed polysaccharides can be used for significantly enhancing the animal immunity, and can also be used for promoting the generation of cell factors, the typing of T lymphocytes and the proliferation of mouse spleen cells so as to ensure that cellular immune reaction can be activated. The functional seaweed polysaccharides disclosed by the invention are natural polysaccharides which are extracted from seaweeds such as large seaweeds and kelps, gulfweeds, grateloupia sparsa, eucheuma muricatum, ulva pertusa kjellm, enteromorpha, gracilaria, ascophyllum nodosum and scytosiphon lomentaria, and can also be low-molecular-weight seaweed polysaccharides or seaweed oligosaccharides which are prepared by degrading the seaweed polysaccharides by virtue of different preparation methods. According to the application disclosed by the invention, a polysaccharide extract of single seaweed or a polysaccharide extract mixture of a variety of seaweeds can be taken as a novel anti-viral and immune enhancing agent to be applied to feeds of livestock, poultry, fish, shrimps and shellfish, and has wide application prospects.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to an application of seaweed polysaccharide. Background technique [0002] With the rapid development of my country's aquaculture industry, especially in recent years, the intensive aquaculture industry has developed rapidly. At present, the prevention and control of livestock and poultry diseases is also facing many problems. Infectious diseases of livestock and poultry can be roughly divided into viral diseases, bacterial diseases, fungal diseases, mycoplasma diseases, etc. according to their pathogenic characteristics. Viral diseases are "difficult problems" that do not have specific drugs and methods of treatment at present. At present, the main measures taken for viral infectious diseases are injections, antiviral drugs and other therapeutic methods. These two methods have certain defects. Vaccination is only effective for a certain virus and does not have a b...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/02A61P31/12A61P37/04A61P31/16
Inventor 李鹏程宋琳陈晓琳邢荣娥刘松于华华秦玉坤李克成李荣峰王雪芹
Owner INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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