Loop-mediated isothermal amplification (LAMP) method for detecting pathogenic bacteria of canker disease of carya cathayensis

A hickory dry rot, ring-mediated isothermal technology, applied in the direction of microbial-based methods, biochemical equipment and methods, microbial measurement/inspection, etc., can solve the health hazards of experimenters, difficult access to primers, bromination Ethidium has huge toxicity and other problems, so as to increase the application value, get rid of the dependence on thermal cycle instruments, and achieve the effect of high sensitivity
CN106868142AActive Publication Date: 2017-06-20ZHEJIANG FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG FORESTRY UNIVERSITY
Publication Date
2017-06-20

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Abstract

The invention discloses a loop-mediated isothermal amplification (LAMP) method for detecting pathogenic bacteria of canker disease of carya cathayensis. An LAMP primer composition is composed of four primers. The invention further discloses an LAMP kit used for detecting pathogenic bacteria of canker disease of carya cathayensis. The kit comprises the previous LAMP primer composition and further comprises 10*ThermoPol Buffer, dNTPs, MgCl2, glycine betaine, hydroxynaphthol blue, 8 U / muL Bst DNA polymerase and ddH2O. According to the method disclosed by the invention, the LAMP primers are designed according to a conserved region fragment sequence of beta-tubulin genes, the fast molecular detection technology system and reaction conditions are optimized, the pathogenic bacteria of the canker disease of carya cathayensis can be subjected to LAMP detection, the method is simple and convenient in reaction process, short in detection period, high in specificity and high in sensitivity, and the detection results can be observed by naked eyes.
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Description

technical field

[0001] The invention belongs to the field of biotechnology, relates to a loop-mediated isothermal amplification primer set and a method for using a loop-mediated isothermal amplification (LAMP) technology for detecting hickory dry rot pathogens, and belongs to early warning for detection, identification and prevention of plant diseases technology field. Background technique

[0002] Hickory dry rot has generally occurred in hickory producing areas such as Lin'an, Chun'an, and Tonglu since 2002, and the affected area has gradually expanded. At present, the area of ​​occurrence has reached 270,000 mu. Severe cases of whole plant withering have occurred, and mild cases have affected growth and caused fruit drop According to calculations, the average output in the occurrence area has decreased by about 24%, which has become a bottleneck for the development of the pecan industry. There are different research reports or speculate that different factors are related...

Claims

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