Regeneration method of larix olgensis

By using mature zygotic embryos of Changbai larch as explants, a highly efficient and rapid regeneration system was established, which solved the problems of long breeding cycle and difficulty in rooting and transplanting of Changbai larch, and achieved the acquisition of highly efficient regenerated plants, laying the foundation for genetic improvement and industrial application.

CN121420892APending Publication Date: 2026-01-30NORTHEAST FORESTRY UNIV
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Patent Information

Application Number
CN202512029568.7
Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Filing Date
2025-12-30
Publication Date
2026-01-30

AI Technical Summary

Technical Problem

The regeneration system of Changbai larch has problems such as long breeding cycle, low efficiency, and difficulty in rooting and transplanting, which limits genetic improvement and industrial application.

Method used

Using mature zygotes of Changbai larch as explants, an efficient and rapid regeneration system was established through steps such as callus induction, adventitious bud induction, adventitious bud elongation, rooting culture, and transplantation. Tissue culture was carried out using specific culture media and light conditions.

Benefits of technology

It has achieved efficient and rapid regeneration of Changbai larch, improved rooting and transplanting rates, shortened the regeneration cycle to about 6 months, and provided technical support for genetic improvement and industrial application.

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Abstract

The invention relates to the technical field of tissue culture, in particular to a regeneration method of larix olgensis. According to the method, mature zygotic embryos of larix olgensis are subjected to callus induction, adventitious bud induction, elongation culture and transplanting. According to the method, the limitation that rooting and transplanting are difficult in the process of a larix olgensis tissue culture regeneration system is broken through, an efficient and rapid tissue culture regeneration system is achieved, the period is only about 6 months, a complete regenerated plant can be obtained, and callus induction, adventitious bud induction and elongation, the rooting rate and the transplanting rate are all improved. Technical support is provided for follow-up development of genetic improvement and industrial application of Larix olgensis trees, a feasible scheme reference is provided for cultivation of a precious new variety of Larix olgensis, and the method is also a breakthrough in the propagation technology level of Larix olgensis.
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