Comprehensive buffer solution for detecting brucellosis by means of colloidal immuno-gold immuno-filtration assay

A brucellosis and immunodiafiltration technology, applied in immunoassays, measuring devices, biological tests, etc., can solve the problems of inability to detect whole blood samples, lack of comprehensive buffer, etc., and achieve low production cost, low cost, Simple to use effects

Inactive Publication Date: 2016-07-20
SHIHEZI UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the current colloidal gold immunofiltration card can only detect the serum obtained by centrifugatio

Method used

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  • Comprehensive buffer solution for detecting brucellosis by means of colloidal immuno-gold immuno-filtration assay
  • Comprehensive buffer solution for detecting brucellosis by means of colloidal immuno-gold immuno-filtration assay
  • Comprehensive buffer solution for detecting brucellosis by means of colloidal immuno-gold immuno-filtration assay

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Experimental program
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Effect test

Embodiment 1

[0030] Embodiment 1: a kind of comprehensive buffer solution is characterized in that comprising: hypotonic buffer solution, nonionic surfactant, amphoteric surfactant, monovalent salt ion, metal ion chelating agent and present negatively charged in hypotonic buffer solution The inert sealing protein, the hypotonic buffer is: barbiturate-hydrochloric acid buffer, the buffer range pH of the hypotonic buffer is: 9, and the effective concentration is 20mmol; the nonionic surfactant is: polyoxyethylene sorbitan Monooleate (TWEEN-80) and lauryl polyethylene glycol ether (brij-35), the effective concentrations of the two selected nonionic surfactants are: 0.15% (w / v); The amphoteric surfactant is: polyethylene glycol octylphenyl ether (Tetronic1307), the effective concentration is: 0.50% (w / v); the monovalent salt ion is: NaCl, the effective concentration is 122mmol; the metal ion chelating agent is: Ethylenediaminetetraacetic acid (EDTA), effective concentration: 32mmol; inert bloc...

Embodiment 2

[0031] Embodiment 2: a kind of comprehensive buffer solution is characterized in that comprising: hypotonic buffer solution, nonionic surfactant, amphoteric surfactant, monovalent salt ion, metal ion chelating agent and present negatively charged in hypotonic buffer solution The inert sealing protein, the hypotonic buffer is: HEPES buffer, the buffer range pH of the hypotonic buffer is: 8.9, and the effective concentration is 29mmol; the nonionic surfactant is: polyoxyethylene sorbitan monolaurate (TWEEN-20) and lauryl polyethylene glycol ether (brij-35), the effective concentration of two kinds of nonionic surfactants selected are respectively: 0.25% (w / v); Amphoteric surfactant For: polyethylene glycol octyl phenyl ether (Tetronic1307), the effective concentration is: 0.20% (w / v); the monovalent salt ion is: KCl, the effective concentration is 106mmol; the metal ion chelating agent is: ethylenediaminetetra Acetic acid (EDTA), effective concentration: 36 mmol; inert occlusive...

Embodiment 3

[0032]Embodiment 3: a kind of comprehensive buffer solution is characterized in that comprising: hypotonic buffer solution, nonionic surfactant, amphoteric surfactant, monovalent salt ion, metal ion chelating agent and in hypotonic buffer solution present negatively charged The inert blocking protein, the hypotonic buffer is: Tris-HCl buffer, the buffer range pH of the hypotonic buffer is: 8.8, and the effective concentration is 38mmol; the nonionic surfactant is: polyoxyethylene sorbitan mono-oil Ester (TWEEN-80) and polyoxyethylene sorbitan monolaurate (TWEEN-20), the effective concentrations of the two selected nonionic surfactants are: 1.25% (w / v); The amphoteric surfactant is: polyethylene glycol octylphenyl ether (Tetronic1307), the effective concentration is 0.70% (w / v); the monovalent salt ion is: NaCl, the effective concentration is 50mmol; the metal ion chelating agent is: B Diaminetetraacetic acid (EDTA), effective concentration: 16mmol; Present negatively charged i...

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Abstract

The invention discloses a comprehensive buffer solution for detecting brucellosis by means of a colloidal immuno-gold immuno-filtration assay. The comprehensive buffer solution is characterized by comprising a hypotonic buffer solution, a non-ionic surfactant, an ampholytic surfactant, univalent salt ions, a metal ion chelating agent and inert occludin which is negatively charged in the hypotonic buffer solution. Compared with the prior art, the comprehensive buffer solution has the advantages of being capable of detecting a whole-blood sample, convenient to operate, accurate in result and the like.

Description

technical field [0001] The invention designs a comprehensive buffer solution for detecting brucellosis by colloidal immunogold immunofiltration method. Background technique [0002] Brucellosis (brucellosis, referred to as brucellosis) is a "reemerging" zoonotic infectious disease that seriously endangers the health of humans and livestock. class of infectious diseases. The disease is prevalent in about 170 countries and regions around the world. In recent years, the human and animal epidemics of brucellosis have shown a trend of recovery around the world, showing a continuous growth trend and intensifying. In my country, except Macao and Taiwan, where the epidemic situation is unknown, all provincial units have reported or discovered cases of brucellosis, and once discovered, the number of cases will increase rapidly. In the past 20 years, the number of brucellosis cases in my country has continued to increase. After 1998, the epidemic situation of human brucellosis has ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/569G01N33/558
CPCG01N33/6854G01N33/558G01N33/56911G01N2469/20
Inventor 石峰陈创夫王远志盛金良张虹杜鲜
Owner SHIHEZI UNIVERSITY
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