Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof

A technology for leather hydrolyzed protein and hydrolyzate, which is applied to the gold-labeled quick detection card for leather hydrolyzate in milk and the field of preparation thereof, can solve the problems of long reaction time, high corrosiveness of reagents, false positives and the like, and achieves simple and convenient preparation method, The effect of simple operation steps and short reaction time

Inactive Publication Date: 2011-01-12
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages are: 1. The reaction time is long, and the acid hydrolysis of the sample only takes 16-24 hours
2. The reagents are highly corrosive, and corrosive substances such as hydrochloric acid, sodium hydroxide, and phenol are used in large quantities, requiring staff to have specialized experimental skills
3. The requirements for instruments are high, and special instruments such as spectrophotometers, constant temperature water baths and heaters are required
But there are

Method used

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  • Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof
  • Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof
  • Dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] Embodiment 1, a kind of quick test card of leather hydrolyzate in milk, such as figure 1 , figure 2As shown, it consists of a sample layer 8, a gold label layer 9, a detection layer 6, a water-absorbing layer 5, a plastic liner 4 and a plastic cover 10; the head of the plastic liner 4 is the front end of the sample layer 8, and the rear end of the sample layer 8 The front end of the gold label layer 9 is stacked and connected, the rear end of the gold label layer 9 is stacked and connected with the front end of the detection layer 6, the rear end of the detection layer 6 is stacked and connected with the front end of the water-absorbing layer 5, and the rear end of the water-absorbing layer 5 is located at the tail of the plastic liner 4; The top of the lining plate 4 is covered with a plastic cover plate 10, the plastic cover plate 10 is provided with a sampling hole 3 and an observation hole 2, the sampling hole 3 is facing the sample layer 8, and the observation hol...

Embodiment 2

[0038] Embodiment 2, the preparation method of leather hydrolyzate gold label rapid detection card in milk, the steps are as follows:

[0039] (1), preparation of colloidal gold-labeled leather hydrolyzed protein solution and gold label layer

[0040] (a) Preparation of colloidal gold solution: operate according to the trisodium citrate-auric acid chloride method; take 100mL of chloroauric acid with a mass percentage concentration of 0.01% and stir and heat to boiling, add trisodium citric acid with a mass percentage concentration of 0.1% Sodium 1.5mL, continue stirring and heating for 15min to obtain a colloidal gold solution;

[0041] (b) Preparation of colloidal gold-labeled leather hydrolyzed protein solution: adjust the pH of the colloidal gold solution to 7.5, add 10 mg of leather hydrolyzed protein to 1 L of colloidal gold solution, shake and mix well, then add 10 g of bovine serum albumin, 15,000 g After centrifugation for 20 min, the resulting precipitate was diluted...

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Abstract

The invention relates to a dot immuno-gold filtration assay (DIGFA) quick detection card for leather hydrolyzate in milk and a preparation method thereof. The detection card is composed of a sample layer, a colloidal gold labeled layer, a detection layer, a water absorption layer, a plastic lining plate and a plastic cover plate. The preparation method comprises the following steps: preparing a colloidal gold labeled leather hydrolyzed protein solution and a colloidal gold labeled layer; preparing a leather hydrolyzed protein multi-clone antibody solution and detection lines on the detection layer; and assembling the detection card. The detection card has the advantages of simple operation and accurate detection result, can be interpreted by naked eyes, does not need complex operation, special equipment and the like, is suitable for on-site casual detection of leather hydrolyzate in milk for milk product enterprises, and inspection and sanitary departments, and has wide application prospects.

Description

technical field [0001] The invention relates to food safety detection, in particular to a gold label rapid detection card for leather hydrolyzate in milk and a preparation method thereof. Background technique [0002] Milk and dairy products are popular foods, and unscrupulous operators often adulterate milk in order to make huge profits, which seriously endangers the legitimate rights and interests of consumers. Key factor. Common milk adulteration methods include: adding water to increase weight, adding salt, nitrate and nitrite to increase specific gravity, mixing antibiotics, alkaline substances and formaldehyde to delay spoilage, adding fat powder to increase fat content; adding urea, Nitrogen-containing substances such as melamine. For this reason, governments at all levels have intensified their rectification efforts, and unscrupulous traders began to add a new type of adulterant—leather hydrolyzate—in order to evade the crackdown. [0003] Leather hydrolyzate is a...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/532
Inventor 张学尧
Owner SHANXI UNIV
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