14 results about "Adulterant" patented technology

Disclosed in the present invention is an Angelicae sinensis radix adulteration quantitative detection method based on terahertz spectroscopy and data fusion. The method comprises the following steps: (1) using Angelicae pubescentis radix powder as an adulterant, doping Angelicae pubescentis radix powder of different concentrations into Angelicae sinensis radix powder, and performing mixing to prepare samples having different adulteration levels; (2) putting each sample into a terahertz system, and measuring each sample to obtain terahertz absorption spectral information and time-domain spectral information of each sample; (3) performing feature extraction on the terahertz absorption spectral information of different samples; (4) using a Gramian angular difference field to convert terahertz time-domain spectra of different samples into images, and extracting image feature information; and (5) using a feature-level data fusion strategy to fuse feature information of terahertz absorption spectra with feature information of Gramian angular difference field images, so as to construct an Angelicae sinensis radix adulteration quantitative detection model. The present invention provides an efficient and reliable solution for Angelicae sinensis radix adulteration detection by using multi-modal feature information.

This invention discloses a CRISPR / Cas-regulated DNA silver nanocluster sensor and its method and application for detecting adulteration in meat, belonging to the field of food safety testing technology. This DNA-templated silver nanocluster sensor introduces a novel tagless fluorescent probe, DNA-AgNCs, as a substrate for CRISPR / Cas12a. After the CRISPR / Cas12a-crRNA complex specifically recognizes the target nucleic acid, its trans-cleavage activity is activated, leading to the cleavage of the DNA template and a significant decrease in fluorescence signal. Detecting adulteration in meat using this DNA-templated silver nanocluster sensor can cleverly convert the concentration of adulteration into a quantifiable fluorescence signal output, achieving quantitative detection of adulterants. It exhibits high sensitivity and specificity, providing a universal and sensitive detection method for meat adulteration.

This invention provides a thin-layer chromatography (TLC) method for detecting and identifying Polygonum bistorta or its adulterants and their formulation granules. The TLC method for detecting Polygonum bistorta or its adulterants includes: spotting the test solution onto a silica gel G TLC plate; developing the plate using a toluene-butyl acetate-formic acid-acetic acid solution at a volume ratio of 10:(8-10):(3-5):(1-2); removing the plate; air-drying the plate; and examining it under ultraviolet light and / or spraying it with a colorimetric reagent and examining it under sunlight. This invention can effectively obtain TLC chromatograms of Polygonum bistorta and its adulterants with a greater number of significant spots. The TLC method is simple to operate, has a rapid detection time, produces a large number of fluorescent spots in the Polygonum bistorta sample, displays rich material information, and provides clear and concise results, allowing for intuitive determination of sample measurement results. The method has strong adaptability; therefore, it can effectively provide the prerequisites for quality control or subsequent identification of Polygonum bistorta and its adulterants.

The application belongs to the technical field of medicinal plants and traditional Chinese medicine identification, and discloses a device for identifying firecracker flower roots and their adulterants, which comprises a liquid nitrogen ball mill, a DNA extraction kit and a PCR amplifier connected in sequence, the liquid nitrogen ball mill is connected with a variable speed motor, and further comprises a sealing assembly, the sealing assembly comprises a first permanent magnet and a sealing ball, the first permanent magnet and the sealing ball repel each other, the first permanent magnet is slidingly arranged in a first through groove, the sealing ball is slidingly arranged in a wedge-shaped through groove, and is connected with a tension spring, the free end of the tension spring is fixedly connected with the wedge-shaped through groove; the first through groove and the wedge-shaped through groove are both provided with a sliding groove, and the first permanent magnet and the sealing ball are slidingly connected with the sliding grooves respectively; the diameter of the sealing ball is not less than the diameter of the opening of the wedge-shaped through groove; the scheme can adjust the supplement amount of liquid nitrogen according to the change of the rotating speed of the ball mill. The application solves the problem that the existing liquid nitrogen ball mill cannot adjust the supplement amount of liquid nitrogen according to the rotating speed of the ball mill.

This application provides a method for detecting adulteration in fermented sausages based on microscopic image analysis. This method accurately extracts and quantifies the microscopic features of meat fibers and connective tissue based on high-resolution microscopic images, and effectively distinguishes adulterants from pure meat using these features. Specifically, it extracts meat fiber regions from an enhanced set of clear images, uses a threshold segmentation algorithm to separate the fiber structure from the background, obtains a fiber region mask, and determines the fiber diameter distribution characteristics. If the classification result indicates adulteration, it extracts an abnormal distribution subset from the combined vector, and uses cluster analysis to group the abnormal features, obtaining a detailed adulteration type result. The detailed adulteration type result is compared and matched with a preset pure meat microscopic feature library, identifying regions with a matching degree below a threshold, and obtaining a final adulteration identification report. This method achieves accurate analysis of the microstructure of meat products and efficient identification of adulteration types, significantly improving the automation and reliability of the detection.

This invention discloses a method for identifying the characteristic chromatograms of Piper kadsura and its adulterants, Schisandra chinensis and Photinia serratifolia. The method includes preparing test and reference solutions, and using high-performance liquid chromatography (HPLC) under specific chromatographic conditions to obtain characteristic chromatograms. Piper kadsura exhibits eight characteristic peaks, Photinia serratifolia shows only four of these peaks, while Schisandra chinensis shows no obvious corresponding chromatographic peaks. By comparing the characteristic chromatograms, Piper kadsura can be quickly and accurately distinguished from its adulterants. This method is highly specific, stable, and reproducible, and is suitable for identifying the authenticity of Piper kadsura medicinal materials, processed slices, standard decoctions, and formulated granules.

A smartphone application and mid-infrared spectrometric system for the analysis of honey. The system enables the determination of syrup and sugar adulterants, moisture content, and floral and geographical origin of honey using advanced machine learning and chemometric techniques. The state-of-the-art system may revolutionize honey purity testing by determining the quantity of the aforementioned parameters (e.g., quantity of syrup and sugar adulterants, percentage of moisture content, and floral and geographical origin of honey) in only 1-2 minutes. The system can be used in situ, thus offering the advantages of portability and convenience.

This invention relates to a method and application for identifying standard decoctions of Dryopteris crassirhizoma and its adulterants. The method includes the following steps: extracting standard decoctions of Dryopteris crassirhizoma and its adulterants using extraction solvents to prepare test solutions; dissolving a reference standard of oxaliplatin AB using a dissolving reagent to prepare a reference solution; and performing high-performance liquid chromatography (HPLC) on the test solution and the reference solution to obtain the content of oxaliplatin AB in the standard decoction of Dryopteris crassirhizoma. This invention establishes a method for determining the content of oxaliplatin AB, a characteristic component of the standard decoction of Dryopteris crassirhizoma, thereby enabling qualitative and quantitative identification of the standard decoction, ensuring its safe use, and for quality control of the standard decoction. The establishment of this method not only provides a basis for establishing quality standards for the standard decoction of Dryopteris crassirhizoma, but also provides an important reference for establishing quality standards for Dryopteris crassirhizoma granules and related traditional Chinese medicine preparations.

This invention discloses a quantitative analysis method for target components in mixtures based on optimal transport cross-domain distribution alignment. This method addresses the significant shortcomings of current mainstream fluorescence spectroscopy-based classification and quantification methods in continuous concentration quantification and generalization of unknown adulterants. Specifically, the types of adulterants appearing during the testing phase must have been covered during the training phase; otherwise, the predictive performance stability will significantly decrease. The method of this invention employs a technical path of "spectral acquisition and preprocessing—distribution alignment—regression prediction," constructing a two-stage analysis workflow of "optimal transport + regression." This achieves robust quantification of the continuous content of target components in a mixture system without relying on prior enumeration of adulterants or using target domain concentration labels. This invention is applicable to other spectral analysis applications involving the recovery of continuous physical quantities, such as food safety testing, food chemical analysis, and material composition analysis.

The invention discloses an application of hyoscyamine alkaloid as a marker for identifying adulterated adulterants in radix aucklandiae, and particularly relates to a method for limiting and detecting a marker hyoscyamine for adulterating toxic drug tripartite in radix aucklandiae. According to the present invention, the anisodus acutangulus powder in the radix aucklandiae is subjected to gradient ratio mixing and extraction, and the hyoscyamine content in the radix aucklandiae-doped anisodus acutangulus mixed extraction liquid is qualitatively and quantitatively detected by using the HPLC method so as to achieve the purpose of controlling the quality and the safety of the radix aucklandiae. The HPLC method is used for qualitatively and quantitatively determining the limit standard of the marker hyoscyamine doped in the radix aucklandiae, and the method has the characteristic of accurately, stably and quickly detecting the doped 3-fraction in the radix aucklandiae medicinal material, so that a scientific basis is provided for ensuring the safety and effectiveness of clinical medication of the radix aucklandiae. Meanwhile, a basis can be provided for impurity limit detection of the toxic and side effects of the radix aucklandiae, and a basis is provided for related regulations of pharmacopoeia and increase of impurity detection items.

The invention relates to a specific chromatogram construction method for distinguishing iron stick yam and counterfeit products thereof based on HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) and application of the specific chromatogram construction method, the iron stick yam and the counterfeit products thereof can be effectively distinguished, and the quality and the medicinal value of the iron stick yam are guaranteed. Then, detecting by using a high performance liquid chromatography electrospray detector under specific chromatographic conditions, and finally, according to a detection result, drawing a standard characteristic chromatogram of the iron stick yam medicinal material as well as the counterfeit product and the reference medicinal material of the iron stick yam medicinal material; the specific chromatogram is applied to quality detection, evaluation and control of the Tiegun yam medicinal material, or is applied to distinguishing the Tiegun yam and counterfeit products of the Tiegun yam. According to the specific chromatogram construction method, the iron stick yam and the counterfeit potatoes, cassava, sweet potatoes and dioscorea nipponica can be identified according to whether the raffinose exists in the chromatographic peak or not, the product quality and medicinal value of the iron stick yam are effectively guaranteed, and the specific chromatogram construction method has practical application value.

The application discloses a characteristic spectrum construction method of medicinal leech and its close relative adulterants. The construction method comprises the following steps: a characteristic spectrum of medicinal leech and its close relative adulterants is established by high performance liquid chromatography, including extracting and preparing test sample solutions from medicinal leech and its close relative adulterants respectively; a control sample solution is prepared, and the test sample solution and the control sample solution are detected by high performance liquid chromatography, so that corresponding characteristic spectra are obtained, and medicinal leech and its close relative adulterants can be quickly distinguished. The medicinal leech control sample is selected from medicinal leech or leech of the Hirudinidae family; the close relative adulterants of the medicinal leech are selected from Limnatis humilis, Haemadipsa rills or Haemadipsa japonica. The method is simple and convenient, has the advantages of simple operation, good separation degree, high precision, good repeatability and good stability, and can realize rapid and accurate distinction of medicinal leech, leech and its close relative adulterants by HPLC.

This invention discloses a detection method and application for *Crassostrea gigas* medicinal material or its processed slices, standard decoctions, and traditional Chinese medicine granules, belonging to the field of analytical detection technology. The detection method includes: enzymatic extraction of *Crassostrea gigas* medicinal material or its processed slices, standard decoctions, and traditional Chinese medicine granules to obtain a test solution; enzymatic extraction and enzymatic digestion of *Crassostrea gigas* reference material to obtain a reference solution; and detection of the test solution and the reference solution using ultra-high performance liquid chromatography-mass spectrometry (UHPLC-MS / MS). The detected ion pairs are 364.21→307.19, 364.21→417.25, 369.71→512.25, and 369.71→448.26. The detection method of this invention has strong specificity and can effectively identify different oyster origins and adulterants through the organic matter of *Crassostrea gigas*, providing a reliable basis for the quality control and evaluation of oysters and their products.

The invention discloses a construction method and application of an aspongopus characteristic peptide spectrum, and the method comprises the following steps: preparing an aspongopus test sample solution and an aspongopus control medicinal material reference substance solution, analyzing by an ultra-high performance liquid chromatography-mass spectrometer, and determining the following six characteristic ion peaks with double-charge parent ions from a peptide spectrogram of the test sample, the characteristic peptide spectrum of the aspongopus is as follows: m / z is 469.263 to 522.312, 617.811 to 731.402, 559.780 to 760.400, 649.800 to 785.350, 623.840 to 430.260, and 789.926 to 1167.596. The characteristic peptide spectrum of the aspongopus can be used for preparing the aspongopus. According to the method, true and false identification of the aspongopus medicinal material, the standard decoction and the formula granules can be simultaneously realized, and adulterants such as jatropha curcas, lysimachia plicata and amblycoris nuda can be effectively distinguished. The method is high in specificity, good in reproducibility, easy and convenient to operate and high in detection efficiency, the technical problem that a traditional identification method is not suitable for a water extraction preparation is solved, and reliable guarantee is provided for quality control of the aspongopus formula granules.