Reagent kit using flow sealing technique for immuno-gold label detection

A detection kit and immunogold standard technology, applied in biological testing, measuring devices, material inspection products, etc., can solve the problems of the influence of nitrocellulose membrane sensitivity, long production time cycle, etc., to avoid sensitivity drop, easy operation, The effect of saving time, effort and cost

Inactive Publication Date: 2002-03-06
GENETECH BIOTECH SHANGHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantages of the existing sealing technology used on the gold standard detection kit: usually when making the detection strip (plate), when the detection strip (plate) is used for sample detection (especially for the detection of blood samples), in order to avoid non-specific adsorption, Need to pre-block the nitrocellulose membrane embedded with antigen (antibody)
The production time cycle of this method is long, and the sealing of the nitrocellulose membrane may have an impact on the sensitivity. This kind of sealing of the nitrocellulose membrane cannot be used for roll-to-roll large-scale production

Method used

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  • Reagent kit using flow sealing technique for immuno-gold label detection

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Effect test

Embodiment 1

[0012] Embodiment 1, the preparation method of the present invention, the blocking agent is added to the sample to be tested, the first step is to mark gold and coat according to the conventional method of making a gold label detection kit, but the sealing step is omitted; the second step is to place the bottom plate 2 1. The upper absorbent paper 1, the nitrocellulose membrane 5 sprayed with antigen (antibody), the colloidal gold pad 3, the lower sample absorbent pad 4 and other components are cut, assembled, and packaged; the third step is to prepare a sealing agent solution or dry powder for standby In the fourth step, when in use, the blocking agent is added to the serum to be tested, and the results are detected and judged according to conventional methods.

Embodiment 2

[0013] Embodiment 2, the preparation method of the present invention, the sealing agent is fixed on the absorbent paper 4 at the lower end, the first step, according to the conventional method of making the gold standard detection kit, mark gold and coat; the second step, the sample absorbent pad 4 (or colloid Gold pad 3) sealing treatment: immerse the sample absorbent pad 4 (or colloidal gold pad 3) in the sealing agent for sealing treatment for 30 minutes, take it out and dry it at 37°C for use; in the third step, put the bottom plate 2, upper absorbent paper 1, The nitrocellulose membrane 5 sprayed with the antigen (antibody), the colloidal gold pad 3, and the bottom sample absorption pad are cut, assembled, and packaged.

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Abstract

A reagent kit using flow closing technique for immuno-gold label detection features that after the specimen to be tested is added to the specimen hole on detection board or the test strip is insertedin the specimen bath, the chromatographic moving of specimen drives the closing agent of specimen pad or colloidal gold pad to nitrocellulose film, so performing seal in flow procedure. Its advantages include simplified process, low cost, no damage to nitrocellulose film and high sensitivity.

Description

technical field [0001] The present invention uses the immunogold standard detection kit of flow sealing technology to relate to a kind of sealing technology and the immunodiagnostic kit and its detection method prepared by this technology, is the rapid diagnostic kit established on the immune colloidal gold technology basis, Because of its convenient and simple operation, safe use, no pollution, fast and accurate diagnosis, it is widely used in immunodiagnosis. technical background [0002] Immunochromatography test strips are a combination of immunoassay and chromatographic techniques, which are particularly convenient and widely used. The effective components of a detection board or a test strip are: upper absorbent paper 1, nitrocellulose membrane 5 embedded with antibody (or antigen) and secondary antibody (or antibody), sprayed with colloidal gold-antigen (antibody) Colloidal gold pad 3, sample absorption pad 4. As shown in the accompanying drawing...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/53G01N33/558
Inventor 张涛李宾彭永济周军龚丹任一萍
Owner GENETECH BIOTECH SHANGHAI
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