Composition for detecting rheumatoid arthritis immune antibody by dot immuno-gold filtration assay

A technology of immune filtration and immune antibody, applied in the direction of anti-animal/human immunoglobulin, measuring device, animal/human peptide, etc., can solve the problem of BiP rheumatoid arthritis without data support, waiting for further independent clinical research , the impact of detection sensitivity, etc.

Active Publication Date: 2011-03-30
SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

64% of patients with rheumatoid arthritis can produce antibodies directly against BiP, and there are reports that it is highly specific to the disease, but there is no data to support the role of BiP in predicting rheumatoid arthritis, and these reports The BiP antibody still needs to be confirmed by independent clinical research (Clinica Chimica Acta 2004, 350, 17-34)
Since the combination of the antibody molecule to be tested and the labeled antibody / antigen is always in the process of adsorption and desorption throughout the detection process, in practical applications, the detection sensitivity is affected by the detection membrane used, which is not conducive to the large-scale clinical application of the product. application

Method used

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  • Composition for detecting rheumatoid arthritis immune antibody by dot immuno-gold filtration assay
  • Composition for detecting rheumatoid arthritis immune antibody by dot immuno-gold filtration assay
  • Composition for detecting rheumatoid arthritis immune antibody by dot immuno-gold filtration assay

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Experimental program
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Effect test

Embodiment 1

[0085] The synthesis of embodiment 1 polypeptide I

[0086] Polypeptides are synthesized by solid-phase synthesis technology using Fmoc chemical method. For the specific steps of this method, see Eur.J.Immunol.1994, 24, 3188-3193; J.Org.Chem.1972, 37, 3404-3409; Huang Weide, Chen Changqing Polypeptide Synthesis, Beijing: Science Press, 1985.

[0087] The formation method and steps of disulfide bonds can be found in the literature: Huang Weide, Chen Changqing Peptide Synthesis, Beijing: Science Press, 1985, p85; Michael W.Pennington Peptide Synthesis Protocols (Methods in Molecular Biology), Humana Press, 1994, p91-169 .

[0088] Through the above steps, the specific sequence of the synthetic polypeptide is:

[0089] Polypeptide I: His-Gln-Cys-Ala-Arg-Phe-Gln-Met-Arg-His-Cit-Arg-Leu-Ile-Arg-Cys-Gly, on which the third and sixteenth positions of Cys pass through the sulfhydryl group Form a disulfide bond, and make the polypeptide form a ring structure, and can simulate the β-...

Embodiment 2

[0090] The synthesis of embodiment 2 polypeptide II

[0091] Polypeptide II was synthesized according to the method disclosed in Example 1: His-Gln-Cys-Ala-Arg-Phe-Gln-Met-Cit-His-Cit-Arg-Leu-Ile-Arg-Cys-Gly, on which the third The Cys at the sixteenth position and the sixteenth position form a disulfide bond through the sulfhydryl group, and make the polypeptide form a ring structure, and can simulate the β-turn structure.

Embodiment 3

[0092] The synthesis of embodiment 3 polypeptide III

[0093] Polypeptide III was synthesized according to the method disclosed in Example 1: His-Gln-Cys-His-Gln-Phe-Arg-Phe-Cit-Gly-Cit-Ser-Arg-Ala-Ala-Cys-Gly, on which the third The Cys at the sixteenth position and the sixteenth position form a disulfide bond through the sulfhydryl group, and make the polypeptide form a ring structure, and can simulate the β-turn structure.

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Abstract

The invention discloses a composition for detecting a rheumatoid arthritis immune antibody by a dot immuno-gold filtration assay, comprising CCP (Casein Calcium Peptide), a high polymer, a microfiltration membrane, a water-absorbing medium and a vector medium, wherein the CCP is covalently combined with the high polymer; and then the high polymer is combined to the microfiltration membrane in a covalence or covalent mode. The composition not only can realize the quick in vitro detection of the rheumatoid arthritis immune antibody, but also is convenient for self-help diagnosis of a patient and provides the reference to knowing the development of disease condition in time.

Description

technical field [0001] The invention relates to a composition for detecting rheumatoid arthritis antibodies, in particular to a composition for detecting rheumatoid arthritis immune antibodies by dot gold immunofiltration method, so as to realize rapid and accurate detection of rheumatoid arthritis immune antibodies detection. Background technique [0002] Clinically, autoimmune diseases mainly include systemic lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, dermatomyositis, polymyositis, systemic (multiple) sclerosis, scleroderma, etc. These diseases were once named " Connective tissue disease", later both abroad and domestically classified them as rheumatic diseases. [0003] Rheumatoid arthritis (RA) is a relatively common systemic autoimmune disease mainly manifested by chronic polyarticular inflammation. Very painful. The incidence of the disease is 0.5-1% worldwide, and the incidence in China is about 0.36%. The age of onset of patients is mostly bet...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/564C07K14/47C07K16/18
Inventor 朱绍荣
Owner SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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