Composition for detecting rheumatoid arthritis immune antibody by lateral chromatography

A technology of lateral flow chromatography and immune antibody, which is applied in biological testing, material inspection products, etc., can solve the problems of high dependence on medical institutions, long testing time, and unfavorable self-monitoring of disease course by ordinary patients

Active Publication Date: 2013-04-03
SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

64% of patients with rheumatoid arthritis can produce antibodies directly against BiP, and there are reports that it is highly specific to the disease, but there is no data to support the role of BiP in predicting rheumatoid arthritis, and these reports The BiP antibody still needs to be confirmed by independent clinical research (Clinica Chimica Acta 2004, 350, 17-34)
A single ELISA test usually requires a large number of samples, and the test takes a long time. The test process also requires professional personnel to operate, and it relies heavily on medical institutions, which is not conducive to the self-help of ordinary patients. monitor

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0092] The synthesis of embodiment 1 polypeptide I

[0093] Polypeptides are synthesized by solid-phase synthesis technology using Fmoc chemical method. For the specific steps of this method, see Eur.J.Immunol.1994, 24, 3188-3193; J.Org.Chem.1972, 37, 3404-3409; Huang Weide, Chen Changqing Polypeptide Synthesis, Beijing: Science Press, 1985.

[0094] The formation method and steps of disulfide bonds can be found in the literature: Huang Weide, Chen Changqing Peptide Synthesis, Beijing: Science Press, 1985, p85; Michael W.Pennington Peptide Synthesis Protocols (Methods in Molecular Biology), Humana Press, 1994, p91-169 .

[0095] Through the above steps, the specific sequence of the synthetic polypeptide is:

[0096] Polypeptide I: His-Gln-Cys-Ala-Arg-Phe-Gln-Met-Arg-His-Cit-Arg-Leu-Ile-Arg-Cys-Gly, on which the third and sixteenth positions of Cys pass through the sulfhydryl group Form a disulfide bond, and make the polypeptide form a ring structure, and can simulate the β-...

Embodiment 2

[0097] The synthesis of embodiment 2 polypeptide II

[0098] Polypeptide II was synthesized according to the method disclosed in Example 1: His-Gln-Cys-Ala-Arg-Phe-Gln-Met-Cit-His-Cit-Arg-Leu-Ile-Arg-Cys-Gly, on which the third The Cys at the sixteenth position and the sixteenth position form a disulfide bond through the sulfhydryl group, and make the polypeptide form a ring structure, and can simulate the β-turn structure.

Embodiment 3

[0099] The synthesis of embodiment 3 polypeptide III

[0100] Polypeptide III was synthesized according to the method disclosed in Example 1: His-Gln-Cys-His-Gln-Phe-Arg-Phe-Cit-Gly-Cit-Ser-Arg-Ala-Ala-Cys-Gly, on which the third The Cys at the sixteenth position and the sixteenth position form a disulfide bond through the sulfhydryl group, and make the polypeptide form a ring structure, and can simulate the β-turn structure.

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PUM

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Abstract

The invention relates to a composition for detecting a rheumatoid arthritis immune antibody by lateral chromatography. The composition comprises a CCP peptide, a high polymer which is covalently bonded with the CCP peptide, and a carrier medium. The composition can realize quick in-vitro detection of the rheumatoid arthritis immune antibody, is favorable for self-service diagnosis of patients, and provides reference for timely knowing the development of disease conditions. The composition has the advantages of improving the sensitivity and specificity of antibody detection, increasing detection accuracy and enlarging detection applicable people by covalently bonding the CCP peptide having different sequences with the high polymer.

Description

technical field [0001] The invention relates to a composition for detecting rheumatoid arthritis antibodies, in particular to a composition for detecting rheumatoid arthritis immune antibodies by lateral flow chromatography, so as to realize rapid detection of rheumatoid rheumatoid arthritis immune antibodies. Background technique [0002] Clinically, autoimmune diseases mainly include systemic lupus erythematosus, rheumatoid arthritis, Sjogren's syndrome, dermatomyositis, polymyositis, systemic (multiple) sclerosis, scleroderma, etc. These diseases were once named " Connective tissue disease", later both abroad and domestically classified them as rheumatic diseases. [0003] Rheumatoid arthritis (RA) is a relatively common systemic autoimmune disease mainly manifested by chronic polyarticular inflammation. Very painful. The incidence of the disease is 0.5-1% worldwide, and the incidence in China is about 0.36%. The age of onset of patients is mostly between 20 and 50 yea...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/53
Inventor 朱绍荣
Owner SHANGHAI RONGSHENG BIOLOGICAL PHARM CO LTD
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