24 results about "Fluoro quinolones" patented technology

The present invention discloses a quinolone compound, its preparation method, and use, belonging to the field of pharmaceutical technology. The present invention provides a novel quinolone compound, including pharmaceutically acceptable salts, hydrates, solvates, polycrystals, or cocrystals thereof, as well as pharmaceutical prodrugs or derivatives thereof. The provided quinolone compound and its derivatives can be used to prepare drugs for treating bacterial infections. The compounds exhibit significant anti-MRSA activity and lack cross-resistance with existing fluoroquinolones.

The invention discloses a magnetic nano-material and application thereof in extraction of fluoroquinolone compounds, and the magnetic nano-material is prepared by mixing an aqueous solution of Fe3O4 (at) SiO2 microspheres with NH2 modified surfaces and an ethanol aqueous solution of fluorinated graphene according to a volume ratio of (2-4): (1-3) and then stirring. The magnetic nano material is used as a magnetic solid-phase extraction adsorbent and can be used for extracting fluoroquinolone compounds in a sample. The magnetic nano material disclosed by the invention not only can efficiently extract various fluoroquinolone compounds in simple matrixes such as water, milk and the like, but also can directly enrich and extract various fluoroquinolone compounds in complex matrixes such as cosmetics and the like without tedious and time-consuming purification steps; and high-throughput detection of various trace fluoroquinolone compounds in a sample can be realized.

The application discloses a method for simultaneously detecting 32 kinds of veterinary drug residues in fecal pollution, and belongs to the technical field of veterinary drug residue detection. The method comprises the following steps: taking a fecal pollution sample, adding Na2EDTA-Mcllvaine buffer solution and acetonitrile for extraction, centrifuging the sample after salting-out to obtain an organic phase, purifying the organic phase through an LPAS purification column, collecting the purified liquid, concentrating and blowing dry, redissolving, filtering, and obtaining a to-be-detected liquid; detecting the to-be-detected liquid by using an ultra-high performance liquid chromatography-triple quadrupole mass spectrometer, gradient elution; and quantitatively detecting 32 kinds of veterinary drugs by using a matrix matching standard curve method. The method is simple in operation and good in purification effect, can effectively remove interfering substances such as fat and protein in the fecal pollution, significantly reduces the matrix effect, is suitable for simultaneously, quickly and accurately detecting 23 kinds of sulfonamides and 9 kinds of fluoroquinolones in pig, chicken, cow and duck fecal pollution, and has good accuracy and precision.

The invention discloses a test strip for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products and a test method, and belongs to the technical field of drug detection.The preparation method comprises the steps that trimethoprim hapten is synthesized; synthesizing a quinolone hapten, a sulfanilamide hapten and a tetracycline hapten; preparing an immunogen and a coating antigen; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pre-treating and assembling. According to the invention, an integrated detection scheme of four types of medicines is constructed, so that residual detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim in aquatic products can be synchronously completed, and different detection tools or batch detection for single type of medicines is not needed. In an actual detection scene of aquatic products, repeated steps of sample treatment and detection operation can be greatly reduced, waste of aquatic product samples caused by repeated sampling is avoided, meanwhile, the overall detection period is shortened, and the rapid quality control requirement of the aquatic products from fishing / breeding to circulation links is met.

The invention discloses a prawn enterohepatic cytozoon disease comprehensive prevention and treatment and green culture method, and relates to the technical field of aquaculture and disease prevention and control. The method comprises the following steps: adding the following four medicines into a prawn feed for feeding: an antiprotozoon triazine compound, wherein the addition amount of the antiprotozoon triazine compound is 0.2-0.3% of the weight of the feed; the addition amount of the fluoroquinolone antibacterial agent is 0.5%-0.8% of the weight of the feed; the adding amount of the medicine for astringing dampness and sores is 0.8%-1% of the weight of the feed; and the addition amount of the promoting coagulant is 0.1-0.2% of the weight of the feed. Through the synergistic effect of quadruple drugs, the immunity and organ repair capacity of prawns are enhanced, the treatment effect is remarkable, and the passive state that no safe and effective chemical drugs or antibiotics can kill EHP spores in prawns at present, and once the EHP spores break out, the EHP spores can only be controlled to spread and reduce loss is changed; the worldwide problem that sick prawns stop growing or grow extremely slowly and only eat but not grow is solved, and the survival rate and the growth rate of the prawns are increased.

The application discloses a kind of livestock product drug residue detection methods based on near infrared spectroscopy technology, it is related to trace detection field, the detection method includes the following steps: S1, livestock product sample is mixed with buffer solution, and obtain homogenate liquid;S2, hydroxylamine hydrochloride solution is added to homogenate liquid, and oscillation reaction is obtained under mild conditions, and reaction liquid is obtained;S3, nanometer gold stick dispersion liquid is added to reaction liquid, and mixed liquid is obtained;S4, near infrared spectrum of mixed liquid is collected;S5, pretreatment is carried out, and local surface plasmon resonance peak position is extracted, and peak position offset is calculated;S6, peak position offset is substituted into quantitative model, and drug residue concentration is calculated.The application realizes the in-situ release of total residue by selective rupture of the covalent bond between drug and protein, and realizes the high-sensitivity detection of sulfonamides, tetracyclines and fluoroquinolones under the condition of extraction-free by combining the signal amplification effect of nanometer gold stick.

The invention discloses a novel treatment method for degrading typical antibiotic fluoroquinolones in water. Fluoroquinolone drugs (FQs) are artificially synthesized antibiotic drugs which are widely applied to clinic. In numerous FQs antibiotics, ciprofloxacin is a typical drug. Therefore, according to the project, the common fluoroquinolone antibiotic ciprofloxacin is selected as a research object, a method for degrading the fluoroquinolone antibiotics in the wastewater by an electrochemical oxidation method is discussed, and the influence of different electrolyte concentrations, pH values, degradation time, current density, electrolysis temperature and other electrolysis conditions on the removal effect is analyzed; a new thought is provided for treatment of the antibiotics in the wastewater.

The application discloses a new non-fluorine quinolone compound and application thereof, and particularly relates to a compound shown in formula (II) and pharmaceutically acceptable salts thereof.

The invention discloses a G-quadruplex nucleic acid aptamer sequence optimization method, an optimized sequence and application of the G-quadruplex nucleic acid aptamer sequence in fluoroquinolone veterinary drug detection, belongs to the technical field of biological medicine, and provides application of the G-quadruplex nucleic acid aptamer sequence in content detection of fluoroquinolone veterinary drugs in food. The G-quadruplex nucleic acid aptamer sequence optimization method is characterized in that the G-quadruplex nucleic acid aptamer sequence is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4. The G-quadruplex nucleic acid aptamer sequence is shown as SEQ ID NO.1, SEQ ID NO.2, SEQ ID NO.3 and SEQ ID NO.4, and the G-quadruplex nucleic acid aptamer sequence is shown as SEQ ID NO.4. According to the present invention, the sequence optimization is performed on the G4 structure of the nucleic acid aptamer, such that the G4 nucleic acid aptamer having high affinity on the FQs target and having the high fluorescence enhancement effect is obtained; the application limitation that the nucleic acid aptamer usually needs to cooperate with the G4 sequence to construct the fluorescence enhanced DNA mimic enzyme is effectively solved, meanwhile, the fluorescence excitation intensity of the DNA mimic enzyme is greatly enhanced, and the sensitivity and accuracy of detecting FQs in food are improved.

The present invention discloses a method and apparatus for treating fluoroquinolone antibiotics in wastewater solutions. The apparatus comprises: a microwave generating component, including a magnetron for generating microwaves; a microwave testing component, including a circulator, a water load, a dual directional coupler, and a microwave power meter; a microwave plasma generating component, including a compression waveguide, a quartz tube, and a working gas loading tank; and a microwave plasma treatment component. The present invention addresses the problems of plasma instability caused by electrode corrosion, short device life, and secondary pollution caused by the introduction of new reagents or catalysts in existing plasma technologies. The microwave plasma generated by the apparatus of the present invention removes norfloxacin, a representative of the fluoroquinolone class, from wastewater. The degradation efficiency is 98.27±1.03% when the treatment time is 6 minutes, and the mineralization rate reaches 68.66±3.21% when the treatment time is 15 minutes.

Provided are antibacterial phages that selectively kill bacteria having a resistance gene or the like. Antibacterial phage for this includes CRISPR-Cas13a with a target sequence that recognizes a specific gene as a target. This target sequence is designed as a spacer sequence for crRNA of 14-28 bases. Specific genes are drug resistance genes and toxins. The drug resistance genes are included in bacterial genomes and / or plasmids having one or any combination of the group including: methicillin-resistant Staphylococcus aureus, vancomycin-resistant Staphylococcus aureus, vancomycin-resistant enterococci, penicillin-resistant pneumococcus, multidrug-resistant Pseudomonas aeruginosa, multidrug-resistant Pseudomonas aeruginosa, carbapenem-resistant Pseudomonas aeruginosa, carbapenem-resistant cephalosporins, third-generation cephalosporin-resistant Pseudomonas aeruginosa, third-generation cephalosporin-resistant E. coli, and fluoroquinolone-resistant E. coli.

The application discloses a rapid detection method for fluoroquinolone drug residues based on a one-antigen two-monoclonal antibody. The application discloses a monoclonal antibody NOR McAb and a monoclonal antibody SAR McAb respectively against the fluoroquinolone drugs norfloxacin NOR and sarafloxacin SAR, a kit containing the monoclonal antibody NOR McAb and the monoclonal antibody SAR McAb, a method for detecting the fluoroquinolone drugs, and application of the monoclonal antibody NOR McAb and the monoclonal antibody SAR McAb and the kit in detection of the fluoroquinolone drugs. The application is based on a coating agent (NOR-1-BSA) and the monoclonal antibody against sarafloxacin and norfloxacin, establishes an indirect competitive ELISA method and a competitive lanthanide fluorescence microsphere lateral flow immunochromatography test strip (LFM-ICS) rapid detection technology for simultaneously detecting at least 13 kinds of fluoroquinolone drugs, and is simple in production and simple, convenient and efficient in detection.

The application discloses a test paper strip and a test method for detecting fluoroquinolones, sulfonamides, tetracyclines and trimethoprim drug residues in aquatic products, and belongs to the technical field of drug detection. A preparation method comprises the following steps: synthesizing a trimethoprim hapten; synthesizing quinolone, sulfonamide and tetracycline haptens; preparing an immunogen and a coating agent; preparing a monoclonal antibody; preparing a gold-labeled antibody; and pretreating and assembling. The application can simultaneously complete the detection of fluoroquinolones, sulfonamides, tetracyclines and trimethoprim residues in aquatic products by constructing a four-class drug integrated detection scheme, and does not need to use different detection tools or batch detection for a single drug. In the actual detection scene of aquatic products, the repeated steps of sample processing and detection operation can be greatly reduced, the waste of aquatic product samples caused by multiple sampling can be avoided, the overall detection period can be shortened, and the rapid quality control demand of aquatic products from fishing / cultivation to circulation can be met.

The present disclosure relates to methods of control, treatment and / or prevention of a neurological disease or disorder in animals, optionally livestock, comprising administering a therapeutically effective amount of a fluoroquinolone compound, optionally pradofloxacin, or a pharmaceutically acceptable salt, solvate, hydrate or hydrate of salt thereof, wherein the neurological disease or disorder is caused by a bacterial infection in the central nervous system. The present disclosure further provides a method of control, treatment and / or prevention of a bacterial infection in the central nervous system in animals, optionally livestock, comprising administering a therapeutically effective amount of a fluoroquinolone compound, optionally pradofloxacin, or a pharmaceutically acceptable salt, solvate, hydrate or hydrate of salt thereof.

The present application relates to the field of medicine, especially relates to the application of quarfloxin in the preparation of anti-tuberculosis drugs. The present application shows that quarfloxin can inhibit the growth of clinical strains and drug-resistant strains of Mycobacterium tuberculosis, and obvious bacteriostatic effect appears in the clinical strains and drug-resistant strains of Mycobacterium tuberculosis, so the application of quarfloxin in the preparation of anti-tuberculosis drugs is provided.

The present application belongs to the field of immune analysis detection, and provides a kind of livestock and poultry product drug residue colorimetric detection kit and its preparation method, the present application adopts sodium carboxymethyl cellulose to coat ferroferric oxide nanoparticles and is coupled with biotinylated immunoglobulin by carbodiimide activation to prepare nano enzyme label, combined with the design of 3,3',5,5'-tetramethylbenzidine-hydrogen peroxide colorimetric system of immunochromatography test strip, realize the high sensitivity rapid detection of fluoroquinolone drugs, the synergistic enhancement of immune capture and enzyme catalytic color development signal amplification, and the balance control of nano enzyme label high load and low background, solve the problem that the sensitivity, repeatability and batch consistency of existing immunochromatography colorimetric detection system are difficult to be optimized simultaneously, and have wide application value in livestock and poultry product drug residue on-site rapid screening.

The application provides a preparation method of a multi-level hole UiO-66(SO3H)2 material, which is prepared by a solvent thermal method assisted by double regulators and combined with in-situ oxidation and acidification, so that the effective pore diameter of the material is expanded while the internal active sites of the material are modified, rich mesoporous transmission channels (mesoporous pore diameters are concentrated in 3-10nm) are constructed, and the problem of mass transfer limitation of macromolecular drugs in the material is solved; in combination with the hydrophilic and negatively charged sulfonic acid groups (-SO3H) introduced by post-modification, strong electrostatic attraction and hydrogen bond effect between the material and fluoroquinolone drug molecules are generated. Strong synergy between physical channels and chemical target sites makes the material exhibit superfast adsorption rate far beyond conventional microporous materials, and solves the problem that trace antibiotics cannot be deeply removed in the prior art.

The present invention belongs to the field of adsorption materials and sample pretreatment technology, and the present invention provides a kind of magnetic carboxyl functionalized covalent organic framework material and its preparation method and application. The present invention is by mixing trimesoyl chloride, 2,4,6-tris (4-aminophenyl) 1,3,5-triazine, triethylamine and organic solvent, reacting to obtain carboxyl functionalized covalent organic framework material; Iron salt, ferrous salt, carboxyl functionalized covalent organic framework material, alkali liquor and water are mixed to obtain mixed solution, reacting to obtain magnetic base functionalized covalent organic framework material. The method is simple, energy consumption is low, synthesis time is short, operation is simple, reaction device is simple and can realize large-scale factory production, and the present invention utilizes carboxyl functionalized covalent organic framework material and adopts high performance liquid chromatography-mass spectrometry to realize fast, reliable fluoroquinolone drug residue analysis method, while also simplifying the covalent organic framework material to the sample pre-treatment process.

The application discloses an aminothiazole oxime amide compound in the field of organic chemistry, as shown in the general formula III: wherein R is hydrogen, methyl, methoxy, nitro, fluorine, chlorine or bromine; R1 is alkyl containing 1-6 carbons. Some target objects of the aminothiazole oxime amide compound prepared in the application have significant antibacterial activity, especially the activity on methicillin-resistant Staphylococcus aureus (MRSA) and fluoroquinolone-resistant Escherichia coli (FREC).

The application discloses a fluoroquinolone compound separation-ionization integrated device and a preparation method and application thereof, wherein the method for preparing the compound separation-ionization integrated device comprises the following steps: performing a silicon hydroxylation treatment on a substrate to obtain a silicon-hydroxylated substrate; performing an amino modification treatment on at least part of the surface of the silicon-hydroxylated substrate to obtain an amino-modified substrate; performing a grafting treatment on the amino-modified substrate to obtain a grafted substrate; contacting a fluoroquinolone template molecule with a first monomer compound, a second monomer compound and a pore-forming agent to perform a prepolymerization reaction, so as to obtain a prepolymer embedded with the fluoroquinolone compound; performing a polymerization reaction on the prepolymer and the grafted substrate to obtain a polymer-coated substrate; contacting the polymer-coated substrate with a crosslinking agent to perform a self-polymerization treatment, so as to obtain a device primary product; and performing an elution treatment on the device primary product to obtain the fluoroquinolone compound separation-ionization integrated device.

New high-efficiency synergistic antimicrobial composition comprising a combination of Triazolo(4,5-d)pyrimidine derivative together with one antibacterial agent selected from the group consisting of fluoroquinolone and pharmaceutical acceptable salts thereof, tetracycline and pharmaceutical acceptable salts thereof or azole and pharmaceutical acceptable salts thereof. Medical device, biomaterial implants or bioprosthesis comprising the new high- efficiency antimicrobial composition incorporated in a coating or bulk distributed.

The application discloses application of fangchinoline in prevention and treatment of salmonella infection. The fangchinoline is an isoquinoline alkaloid and is a host-directed therapy natural compound. The application discloses that the fangchinoline has a strong effect on treatment of salmonella infection diseases in vivo and in vitro, and the effect is specifically shown as that the fangchinoline can effectively reduce the bacterial amount of intracellular infection bacteria. The fangchinoline administration can significantly reduce the pathogenic bacteria load in the liver, spleen, colon, cecum and ileum of mice, and reduce the pro-inflammatory factor level of the organism. The safety in vivo and in vitro shows that the fangchinoline has no any toxic side effect under the test dose. In addition, the fangchinoline has significant synergistic antibacterial activity in combination with fluoroquinolones, which is shown as reduction of intracellular salmonella infection bacteria number and reduction of the pathogenic bacteria load in the liver, spleen and colon of mice. The application provides a new perspective for development of a new anti-infection drug, and also provides a new idea for prevention and control of the increasingly serious salmonella infection.