219 results about "Fluoro quinolones" patented technology

A pharmaceutical composition comprising a fluoroquinolone such as ciprofloxacin, cyclodextrin, and a hydroxy acid is described. The composition may be an aqueous composition, with such aqueous compositions preferably having a pH between 5 and 7. In some preferred embodiments, the composition further comprises a soluble polymer.

The invention discloses a preparation method of anti-fluoroquinolone rabbit monoclonal antibodies and application thereof. Six fluoroquinolone medicaments are chosen in the invention and coupled with Bovine serum albumin (BSA) to synthesize immunoantigens respectively. The above six immunoantigens are injected subcutaneously in the backs of immune rabbits after being mixed together. The anti-fluoroquinolone rabbit monoclonal antibodies are obtained by hybridoma technique. Rabbit monoclonal antibodies, which are superior to rat monoclonal antibodies, are prepared in the invention. More antigenic determinants are identified when rabbits are chosen as the experimental animal instead of rats. In addition, more integrated experiments can be carried out in rabbit spleens, since the spleens of rabbits are larger than that of rats. The invention provides the possibility of high throughput screening for fusion cells. The anti-fluoroquinolone antibodies of wide-adaptability in the invention has good detection effect on fluoroquinolones, such as enrofloxacin, ofloxacin, Difloxacin, Pefloxacin, Fleroxacin, Danofloxacin, etc., thus making it applicable to fluoroquinolone residue detection on food, forage and environmental samples.

The present invention relates to a high-throughput detection chip for drug resistance gene of bacteria, and an application thereof. The detection chip comprises 117 gene probes, the drug resistance gene probes are selected from 17 categories of drug resistance genes, which comprise extended spectrum beta-lactamase, cephalosporinase, carbapenemase, integrase gene, tetracycline resistance gene, aminoglycoside resistance gene, disinfectant resistance gene, erythromycin resistance gene, macrolide efflux gene, vancomycin resistance gene, multidrug resistance efflux pump gene, mupirocin resistance gene, sulfanilamide resistance gene, tylosin resistance gene, fluoroquinolone resistance gene, chloramphenicol acetyltransferase and commonly-used genetic engineering vector resistance gene. The chip is adopted for detecting the resistance gene of the pathogenic bacteria. The chip is characterized in that: the chip comprises (1) 117-oligonucleotide probe composition and quality control probes of 17 categories of the drug resistance genes; (2) probe arrays, wherein the oligonucleotide probes are solidified on the vector material through arm molecules to form the probe arrays.

The present invention relates to methods for preventing or treating infectious diseases caused by extracellular microorganisms, such as bacteria and fungi, by systemically administering to a patient a compound containing gallium. The extracellular microorganisms targeted by the present methods include methicillin-resistant Staphylococcus aureus (MRSA), vancomycin-resistant Enterococcus faecalis (VRE), E. coli O157:H7, fluoroquinolone-resistant Salmonella typhi, and the like. Furthermore, in the present methods, gallium compounds can be co-administered with one or more conventional antimicrobial agents to treat infectious diseases with reduced risks of creating multi-drug resistant pathogens. The methods of the present invention is also applicable to those microorganisms, such as ulcer-causing Helicobacter pylori, complete eradication of which so far has been difficult to achieve.

The invention belongs to the technical field of detection of enrichment of remained antibiotics in a water body environment, and particularly relates to a synchronous detection method of tetracyclines, fluoroquinolones and sulfonamide antibiotics remained in the water body. The detection method comprises the following steps: (1) performing water sample pretreatment; (2) performing small-column enrichment of target antibiotics by using solid-phase extraction; (3) detecting on a machine by using high performance liquid chromatography and tandem mass spectrum. According to the method, once the sample feeding is carried out at one time, multiple antibiotics in the water body can be detected at the same time, the time consumption of a detection process is reduced, the detection accuracy and sensitivity are increased, the detection limit is low, and the condition of the trace amount of remained antibiotics in the water body environment can be detected; meanwhile, the detection method is convenient to operate and low in environment toxicity and is low in using amount of organic reagents.

The invention discloses a solid phase extraction and HPLC-fluorescence detection method for fluoroquinolones antibiotics. The method comprises the following steps of passing a pretreated water sample through an activated solid phase extraction column; eluting the solid phase extraction column with a Na2EDTA aqueous solution; then vacuumizing; and finally, eluting the water sample for three times by a concentrated ammonia methanol solution with a concentrated ammonia volume percent concentration of 6 percent by 0.5-2mL each time. An obtained eluent is blow-dried to 0.1-0.4mL in air flow, water is added to reach a constant column of 1.0mL so as to obtain a test sample, and then HPLC-fluorescence detection is carried out. By adopting the solid phase extraction-HPLC-fluorescence detection technology and utilizing a conventional analytical instrument, the method can simultaneously measure the contents of four typical fluoroquinolones antibiotics of ofloxacin, norfloxacin, ciprofloxacin and enrofloxacin in the water sample. The method has simple operation and low cost and can be applied to the measurement of the ultratrace of four typical fluoroquinolones antibiotics in waste water and surface water.

The invention relates to a method for separation enrichment and detection of trace fluoroquinolone antibiotic residues in a water environment. According to the method, 2.0-4.0L of a water sample is subjected to solid-phase extraction, and then analysis and detection are carried out by a liquid chromatography-mass spectrometry instrument. Being able to well separate and detect a plurality of fluoroquinolone antibiotic substances, the invention has the advantages of low detection limit, high recovery rate, high sensitivity, good selectivity and simple operation, etc., and has substantial effects in the detection study of trace fluoroquinolone antibiotic substances in the water environment.

This invention relates to a method of treating otitis externa using a topical combination medication, including one or more antifungal agent such as, for example fluconazole, voriconazole, itraconazole, clotrimazole, amphotericin B, caspofungin, micafungin, terbinafine, naftifine, butenafine, amorolfine, ravuconazole, posaconazole, flucytosine, econazole, enilaconazole, miconazole, oxiconazole, saperconazole, sulconazole, terconazole, tioconazole, nikkomycin Z, anidulafungin (LY303366), nystatin, pimaricin, griseofulvin, ciclopirox, haloprogin, tolnaftate, and undecylenate and one or more antibacterial agent such as neomycin sulfate, polymyxin B sulfate, colistin sulfate, gentamycin, tobramycin, chloramphenicol, Ciprofloxacin, Ofloxacin, a penicillin compound, a cephalosporin compound, a macrolide compound, a fluoroquinolone compound, streptomycin, or kanamycin.

Belonging to the technical field of biochar preparation, the invention discloses preparation of Fe-Zn and phosphate modified sludge biochar and application of the same in removal of fluoroquinolones antibiotics from water. The preparation method includes: dewatering and drying the sludge from a secondary sedimentation tank of a municipal sewage treatment plant, then performing pyrolysis by a hightemperature tubular furnace, then utilizing Fe-Zn and phosphate for modification, and finally conducting two calcining to obtain Fe/Zn+H3PO4-SBC. The Fe/Zn+H3PO4-SBC prepared by the invention has adsorption capacity on fluoroquinolones antibiotics ciprofloxacin, norfloxacin and ofloxacin up to 25.43-88.73mg/g. The method provided by the invention adopts municipal sludge as the raw material, reduces the production cost, realizes the resource utilization of municipal sludge and efficient removal of antibiotics from water, also has the advantages of simple operation, low cost and the like, and isa potential removal method for fluoroquinolones antibiotics in water.

The invention relates to a monoclonal antibody as well as a preparation method and application thereof, in particular to a monoclonal antibody of fluoroquinolone drugs and application thereof, belonging to the technical field of immunochemistry. The monoclonal antibody of fluoroquinolones 4G11' is produced from a mouse hybridoma cell strain 4G11, and the subtype of 4G11 is an IgG1 type. The invention has the advantages that the monoclonal antibody 4G11' produced from the mouse hybridoma cell strain 4G11 has high cross reaction rate with various fluoroquinolone medicines and can be produced in large quantity and used for preparing an enzyme linked immunosorbent assay kit and colloidal gold test paper for detecting the fluoroquinolone medicines, achieves the purpose of quickly and sensitively detecting the residual fluoroquinolone medicines in milk, muscle and aquatic products and provides a basis for developing a kit for detecting various residual fluoroquinolone medicines.

The invention provides a preparation method and an application method of a fluoroquinolone antibiotic medicine substitute template molecularly imprinted polymer. According to the invention, substitute template molecules, a cross-linking agent, a functional monomer, and an initiator are dissolved in a pore-forming solvent, such that a pre-polymerization solution is prepared; a polymerization reaction is carried out for 24-48h under a temperature of 50-70 DEG C; a white block-shaped polymer obtained in the polymerization reaction is ground, sieved, and settled, such that polymer particles with a particle size of 45-63[mu]m are obtained; the obtained polymer is subjected to Soxhlet extraction or ultrasonic extraction sequentially by using methanol/acetic acid and methanol as extraction solvents, such that residual substitute template molecules and interfering substances are removed, and the substitute template molecularly imprinted polymer is obtained. The substitute template molecularly imprinted polymer has high specific selectivity and enrichment capability upon fluoroquinolone antibiotics. With the material, an imprinting effect is ensured, and a template leakage problem is solved. The polymer can be used in separation, enrichment and purification of fluoroquinolone antibiotics in complex substrate.

Citrus canker and brown spot are common diseases of citrus. At present, few drugs are available to prevent and control the two diseases and have certain defects. Amino groups at the 7th positions of fluoroquinolone drugs are linked with an active fragment by means of a connecting structure so as to obtain compounds shown in a formula I or a formula II. Experiments prove that the compounds providedby the invention have effects of preventing and controlling the citrus canker and the brown spot and have very good application prospect.

The invention discloses a multi-residue colloidal-gold rapid detection kit and a detection method and application thereof, belonging to the field of immunology. The kit provided by the invention mainly comprises a multi-residue colloidal-gold rapid detection card; the detection card comprises a detector bar and a plastic card shell, and the detector bar is composed of a sample pad, a colloidal-gold antibody binding pad, a nitrocellulose membrane and a water absorbing pad. The colloidal-gold antibody binding pad comprises a colloidal gold labeled gentamycin monoclonal antibody, a colloidal gold labeled kanamycin monoclonal antibody and a colloidal gold labeled fluoroquinolone monoclonal antibody. The central part of the nitrocellulose membrane is coated with a detection line and a control line, wherein the detection line is arranged to be a protein conjugate and the control line is a goat anti-mouse IgG monoclonal antibody. The rapid detection kit provided by the invention can realize simultaneous detection of a plurality of residues only through simple pre-treatment, the whole operation process is simple, the kit is convenient to carry, result determination is rapid and accurate, and the kit is applicable to on-site monitoring and to qualitative screening of considerable samples.

The invention discloses a detection and analysis method for quantitatively detecting six FQs (fluroquinolones) in chicken manure. The method comprises the steps as follows: (1) freezing and drying a chicken manure sample; (2) crushing and sieving the chicken manure sample; (3) adding EDTA-Mcllvaine and an acetonitrile mixed extracting agent, and performing vortex even mixing and ultrasonic treatment; (4) obtaining a supernatant serving as an extracting solution through centrifugation; (5) removing an organic solvent and filtering the extracting solution with a filter membrane; (6) subjecting a filtering solution to an HLB (hydrophilelipophilebalance) column, pumping the filtering solution from the column, cleaning the column with a 5% methanol solution, finally eluting a target antibiotic with methyl alcohol, and collecting an eluent; (7) drying the eluent, determining the volume with acetonitrile/0.7% phosphoric acid, and fully dissolving and filtering the solution for quantitative detection. According to the method, simultaneous detection of the six FQs is realized, the method has the characteristics of low cost, easiness in operation and the like, an optional method for detecting the FQs type antibiotics in complex matrixes is provided, and the method is low in both detection limit and quantitation limit and high in recovery rate.

The invention relates to a novel method of extracting fluoroquinolone type medicines in faeces of livestock and poultry. The method includes 1) grinding a freeze-dried sample of faeces of livestock and poultry, and sieving; 2) weighing 0.50-1.00 g of the sample, adding into a centrifuge tube, adding 5-10 mL of a calcium chloride-hydrochloric acid buffer solution having a concentration of 50%, performing vortex mixing for 1 min, performing ultrasonic extraction for 15-30 min, centrifuging at a speed of 4500 r/min for 10 min, and collecting the supernate; 3) separately subjecting standard samples of one or more than two of the fluoroquinolone type medicines to qualitative analysis by a high performance liquid chromatography instrument, and determining retention time; 4) creating a standard curve for each fluoroquinolone type medicine compound by adopting peak area as a vertical coordinate and adopting a concentration (ng/mL) corresponding to each fluoroquinolone type medicine as a horizontal ordinate; and 5) measuring the content of each target compound in the sample to be measured by high performance liquid chromatography, determining the type of each target compound according to the retention time, and determining the content of each target compound according to the corresponding standard curve. The method is good in repeatability and high sensitivity.

The invention discloses a method for measuring residual quantities of four fluoroquinolone medicaments in animal food, and relates to a liquid chromatography method for measuring the residual quantities of medicaments in animal food. The invention aims to provide a measuring method which is simple and quick in operation, high in automation degree, high in extraction efficiency, high in recovery rate and good in repeatability. The method for measuring the residual quantities of the four fluoroquinolone medicaments in the animal food comprises the following steps: sample preparation; sample pretreatment: accurately weighing a sample, mixing the sample and kieselguhr uniformly, transferring the mixture to an extraction tank, placing the extraction tank on a quick solvent extraction instrument to perform extraction, adding n-hexane and diethyl ether into the obtained extract to remove lipid impurities, performing violent oscillation, standing for demixing, removing the n-hexane-diethyl ether layer, repeatedly operating for two times, transferring the solution at the lower layer to a concentration bottle, rotationally steaming the solution in a rotary evaporator till the solution is dried, dissolving the dregs by using a mobile phase, and filtering the solution through a filter membrane for high performance liquid chromatography (HPLC) analysis; preparation of a standard solution; and efficient liquid chromatography measurement.

The invention discloses a universal hapten of fluoroquinolone medicines, artificial antigen, a broad-spectrum monoclonal antibody, a preparation method and an application, which designs and synthesizes the universal hapten containing a fluoroquinolone medicine mother nucleus structure, the artificial antigen, and the broad-spectrum monoclonal antibody capable of identifying various fluoroquinolone medicines. The universal hapten of fluoroquinolone medicines, the artificial antigen and the broad-spectrum monoclonal antibody can be used for immunoassay and screening of the fluoroquinolone medicines, the detection efficiency is enhanced, the detection time is shortened and the detection cost can be reduced.

The invention provides a test paper for detecting fluoroquinolone drugs. The test paper includes a sample absorption pad (1), a conjugate release pad (2) and a conjugate release pad (3), a reaction membrane (4), a water absorption pad (5) and a PVC (8) baseboard. The reaction membrane is provided with a detection line (6) coated with a fluoroquinolone-carrier protein conjugate and a quality control line (7) coated with a rabbit anti-sheep anti-antibody. The conjugate release pad (2) is coated with a sheep anti-mouse anti-antibody-colloidal gold marker, and the conjugate release pad (3) is coated with a fluoroquinolone monoclonal antibody. The invention also provides a method for applying the fluoroquinolone test paper to detection of fluoroquinolone drugs. The test paper provided by the invention can be used for detecting fluoroquinolone drug residue in cosmetics, and has the characteristics of simple operation, high sensitivity, fast detection speed and low cost, thus being suitable for screening of a large number of samples and field monitoring.

The invention discloses a test method and a sample pretreatment method of flouroquinolone medicine in organic fertilizer. The sample pretreatment method comprises the steps of sampling organic fertilizer, adding EDTA buffer solution with 9.0-11.0 pH level, shaking thoroughly, waiting the solution turning static, taking a first supernatant liquid; adjusting the pH level of the first supernatant liquid to 3.5-4.5, adding organic solvent, shaking thoroughly again, waiting the solution turning static, taking out a second supernatant; the choice for the organic solvent shall select acetomitrile or acetone; adding adsorbing agent in the second supernatant liquid, shaking thoroughly, waiting the solution turning static, taking a third supernatant liquid and blowing dry, thus the pretreated sample of the organic fertilizer is obtained. The test method of flouroquinolone medicine in an organic fertilizer is to use the sample pretreatment method to obtain the pretreated sample of the organic fertilizer, namely the test sample, and directly applying one or more presently available test methods of flouroquinolone medicine for detection. The method has the advantages of simple, fast operation and high precision of test results which signifies enormous application potential.

The present invention relates to phosphonated fluoroquinolones, antibacterial analogs thereof, and methods of using such compounds. These compounds are useful as antibiotics for prevention and/or the treatment of bone and joint infections, especially for the prevention and/or treatment of osteomyelitis.

The invention belongs to the field of medicinal chemistry, and in particular relates to fluoroquinolone derivatives and an application thereof. The compounds shown in a formula (I) are obtained by structural modification of fluoroquinolone medicines. The compounds provided by the invention can not only treat the infection caused by mycobacterium tuberculosis and common bacteria, but also can has activity inhibition effects on bacterial persister, citrus pathogenic bacteria, nicotinamide N-methyltransferase (NNMT) and interleukin IL-17 PPI; and the compounds have a simple and easy preparation process and mild conditions, a plurality of the compounds with enhanced antibacterial activity, improved water solubility and reduced toxic and side effects are obtained, and the compounds are expectedto reduce the dosage, shorten the treatment cycle and improve patient compliance, thereby providing novel molecular types and research ideas for medicines of tuberculosis and other diseases.

The invention relates to a method for detecting escherichia coli fluoroquinolone-resisting gyrA/parC gene point mutation, and belongs to the technical field of gene mutation detection. The method comprises the following steps: (1) extracting Escherichia coli genome DNA; (2) by taking the target gene sequence of E.coli ATCC25922 as a template, respectively designing and synthesizing fluorescence labeling probes gyrP and parP covering gyrA (Der codon83 and Asp codon87), and primers; (3) drawing a PCR amplification and probe melting curve of a strain DNA sample to be detected, adding DNA polymerase which is in thermal stability and is lack of 5' nuclease activity to prevent the probes from hydrolysis, and taking E.coli ATCC25922 as control; (4) judging whether point mutation happens or not. Due to adoption of the fluorescent probes, the method is high in specificity, and the detection can be completed within 2 hours.

The invention discloses a method for synthesizing hollow molecularly imprinted polymer through a silicon dioxide matrix sacrifice method, belonging to the technical field of analysis and detection. In order to find a way of scientifically and accurately detecting and quantifying excessive residues of FQs (Fluoroquinolones) on animal derived food such as farm animals, fowls and aquatic animals, the silicon dioxide matrix sacrifice method is adopted for synthesizing the hollow molecularly imprinted polymer, namely, a silica gel template sacrifice method is adopted, the hollow material with high mass transfer kinetics, high selectivity and high appetency is synthesized as an adsorbent to enrich and selectively separating FQs drugs, and a very good effect is obtained by combining the method with HPLC-UV detection.

The invention discloses novel fluoroquinolone compounds shown in a structural general formula (I). R1 is difluoromethyl or trifluoromethyl; R2 is a substituted piperazine (1), piperidine (2), pyrrolidine (3), (5) or 3-aminopyrrole (4) derivative which is connected with carbostyril through a C-N bond; and the chiral center of 2-bit carbon in the formula (I) can have laevorotatory, dextral and racemic configurations. The invention also relates to a method for preparing the compounds and application of the compounds and a medicinal composition to the treatment of diseases of urinary tracts, intestinal tracts, respiratory tracts, skin soft tissues and enterocoelia.

The invention relates to a method for preparing fluoroquinolone antibacterial agents, namely besifloxacin hydrochloride. The method comprises the step of performing three-step reaction of condensation and the like by taking 1-cyclopropyl-6,7-difluoro-8-chloro-1,4-dichloro-4-oxo-quinoline-3-carboxyl-O3, O4-boron diacetate carboxylate and (R)-3-triphenylmethyl amino-hexahydro aza or salt thereof as raw materials to prepare the besifloxacin hydrochloride. The method is stable, reliable and high in efficiency, and is suitable for industrial production.