35 results about "Fangchinoline" patented technology

The invention relates to the field of detection on traditional Chinese medicine quality, relates to a method for rapidly detecting index composition in Chinese herbal medicine compound preparation, and especially relates to a rapid detection method for index composition in a Chinese herbal medicine compound preparation radix-stephaniae-tetrandrae radix-astragali soup. According to the method, methanol with the concentration of 50% is employed for ultrasonic extraction, and an HPLC-DAD process is applied to simutaneously determine the content of fangchinoline, tetrandrine, calycosin-7-glucoside, liquiritin and glycyrrhizic acid in radix stephaniae tetrandrae, radix astragali and radix glycyrrhizae in the radix-stephaniae-tetrandrae radix-astragali soup at one time. The method solves the problem that quality control is simultaneously performed on stephaniae tetrandrae, radix astragali and radix glycyrrhizae in the radix-stephaniae-tetrandrae radix-astragali soup, and avoids time and reagent waste caused by multiple detection on the five index compositions. The method is simple in operation and high in working efficiency and precision.

The invention belongs to the technical field of pharmacy, and particularly discloses a stephania hainanensis total alkaloid extract extracted from stephania hainanensis tubers and a preparation method thereof. The preparation process is as follows: material (stephania hainanensis dry tubers); grinding; soaking in a moderate amount of 0.01 to 10 percent of hydrochloric acid or sulfuric acid; 1 to 4 times of ultrasonic extraction; filtration; storng-acid cation exchange resin purification; solvent recovery for drying; and stephania hainanensis total alkaloid extract. The extract mainly comprises L-tetrahydropalmatine, palmatine, fangchinoline, hanfangchin and other active ingredients, the percentage by weight of stephania hainanensis total alkaloid is greater than or equal to 70 percent, the percentage by weight of the palmatine is greater than or equal to 20 percent, and the percentage by weight of the fangchinoline is greater than or equal to 40 percent. Reports on the stephania hainanensis total alkaloid extraction process are not found in related literatures so far, the operation flow of the method is simple, the purity of the product is high, the efficacy is remarkable, and the method disclosed by the invention is applicable to industrial production and the exploitation and utilization of local stephania hainanensis resources.

The invention relates a method for separating and purifying fangchinoline and tetrandrine from stephania tetrandra. The stephania tetrandra is taken as a raw material, and the method comprises the following steps of: (1) preparation of crude extract; (2) pre-separation of acid dissolution and alkaline sedimentation; and (3) purification of a high-speed reverse flow chromatograph to obtain the fangchinoline and tetrandrine with purities over 98%. According to the method disclosed by the invention, alkaloid in the stephania tetrandra medicinal material is extracted by alcohol, pre-separated by the acid dissolution and alkaline sedimentation and purified by the high-speed reverse flow chromatograph, and the technical process is green and environemtal-friendly and does not have serious damageto the environment.

The invention discloses a compound algistat which is prepared from berberine, fangchinoline and sanguinarine hydrate. The invention also discloses a preparation method and application of the compound algistat. The compound algistat is an environment-friendly algistat, through the combination and advantage complementary of multiple typical alkaloid compounds, the multiple algae causing algae blooms can be effectively suppressed at the same time, thereby greatly improving the algicidal efficiency. The compound algistat provided by the invention has an effective inhibition rate of 80% to 92% or more within 96h on three kinds of green algae such as microcystis aeruginosa, chlorella pyrenoidosa and scenedesmusobliqnus.

The invention discloses a preparation method for tetrandrine and fangchinoline and belongs to the technical field of drug preparation. Lipotropy tertiary amine total alkali is adopted, and the preparation method comprises two steps that firstly, a medium pressure silica gel chromatography column method is used for separation, and elution is carried out through petroleum ether-ethyl acetate-diethylamine; secondly, purification is carried out through a preparation high performance liquid chromatography, methyl alcohol-water serves as a mobile phase, and finally, eluent is concentrated and evaporated to dryness. The method has the advantages that operation is convenient and quick and purity and the extraction rate are high.

The invention provides application of fangji root extract and fangchinoline in pharmacy. Fangji root extract and fangchinoline are used as components of an acetylcholinesterase inhibitor, and the acetylcholinesterase inhibitor is used for preparing a medicine for preventing and treating Alzheimer disease. The invention provides the application of fangji root extract and fangchinoline in pharmacy,provides a new drug candidate source for preventing and treating Alzheimer disease, and has important significance in the aspects of improving the clinical treatment effect of the Alzheimer disease and reducing the toxic and side effects of drugs.

The invention discloses a sulfonyl fangchinoline compound as well as a preparation method and application thereof. The sulfonyl fangchinoline compound has a structure as shown in a formula (I) or a formula (I'). According to the formula (I) or formula (I'), R is selected from alkyl, aryl, benzyl, heterocyclyl, substituted alkyl, substituted aryl, substituted benzyl and substituted heterocyclyl; the substituent group of the substituted alkyl is halogen or nitro; the substituent group of the substituted aryl, the substituent group of the substituted benzyl and the substituent group of the substituted heterocyclyl are one or more of halogen, alkyl and nitro; Y is selected from one-molecule inorganic acid and organic acid or two-molecule inorganic acid and organic acid. The compound has a bisbenzylisoquinoline parent nucleus structure, has sulfonyl characteristics, has a good XOD inhibition effect, and can be used for treating and improving gout, reducing uric acid and the like.

The present invention relates to a preparation extracted from Stephania tetrandra S. Moor and its use for manufacture of medecine for enhancing the sensitivity or effect of chemotherapy, enhancing the sensitivity of radiotherapy, improving the level of leukocytes in body, and having antineoplastic and antianaphylaxis effects. Said preparation is mainly composed of tetrandrine and fangchinoline with the ratio of 9:1 to 8:2 (moles), which is obtained by the following process: (1) the powder or piece of Stephania tetrandra S. Moor is extracted with dilute acid with the cool acid extract being collected; and washed with water with the aqueous extract being collected; then the cool acid extract and aqueous extract are combined, adjusted with base pH 10 to 12, kept overnight; (2) the fluid of Step (1) is filtered, the solid dried, pulverized to give Powder 1; (3) Powder 1 is heated under reflux in alcohol for 30 min.; (4) the alcohol extract from Step (3) is concentrated, kept, filtered to give Solid 2; (5) Solid 2 is dissolved in warm ammoniac ethanol, kept for crystallization. The present invention also relates to the use of tetrandrine and the pharmaceutically acceptable salt thereof for manufacture of medecine for improving the level of leukocytes in body.

The invention provides a method for extracting tetrandrine and fangchinoline from radix stephaniae tetrandrae. The method comprises the following specific steps: pulverizing dry radix stephaniae tetrandrae, adding bacillus subtilis and yeast for fermentation, performing alcohol extraction, filtration and concentration to a concentrated liquid, adding hydrochloric acid to adjust the pH value, and performing butanol extraction to obtain an aqueous phase A and a butanol extracting solution A; adding sodium hydroxide and hydrochloric acid into the butanol extracting solution A to adjust the pH value, and performing butanol extraction, concentration, sodium carbonate solution dissolving, filtration and ethanol crystallization to obtain the tetrandrine; adding sodium hydroxide into the aqueous phase A to adjust the pH value, and performing butanol extraction, concentration, sodium carbonate solution dissolving, filtration and ethanol crystallization to obtain the fangchinoline. The method can effectively increase the yield of alkaloid of the radix stephaniae tetrandrae, reduce the difficulty of wastewater treatment and improve the utilization rate of radix stephaniae tetrandrae resources.

The invention discloses an application of fangchinoline for preparing medicine for treating or preventing HIV. The fangchinoline can dosage-dependently inhibit the copying of HIV-1 under in-vitro condition, and can inhibit the copying of HIV-1 by the percentage of more than 97% under nontoxic concentration on cell. The fangchinoline acts on the later period in a virus copying period, and the action mechanism is different from all the antiviral drugs on the market, and the effect target is new. The fangchinoline is taken as an effective component of traditional Chinese medicinal materials such as Radix Stephaniae Tetrandrae, the source is wide, the fangchinoline has the advantages of safety and low toxicity, and has wide prospect for exploitation of anti-HIV-1 drugs.

The invention relates to the field of medicine, in particular to a new medical use of a bisbenzylisoquinoline alkaloid compound. The invention provides an application of fangchinoline in the preparation of a medicine for preventing and treating ulcerative colitis. Preferably, fangchinoline is used as a unique active ingredient for preparing the medicine for preventing and treating ulcerative colitis. Fangchinoline can be used for preparing the medicine for preventing and treating ulcerative colitis.

The invention provides a technology for inhibiting activity of acetylcholin esterase. The technology comprises a pharmaceutical composition for inhibiting the activity of acetylcholin esterase, an Alzheimer disease prevention and treatment medicine, a production method of the pharmaceutical composition and a production method of the Alzheimer disease prevention and treatment medicine. The pharmaceutical composition comprises fangchinoline and berberine at a mole ratio of 1:5-2:1. The inventors combine fangchinoline and berberine by reference to a clue and a method of compound compatibility oftraditional Chinese medicines; an obvious synergic compatibility effect can be generated in an aspect of inhibition of acetylcholin esterase; and the use dosage and toxicity of a single medicine are reduced.

The invention belongs to the technical field of chemical pesticide synthesis, and particularly relates to a compound fangchinoline-carbamate derivative with bactericidal activity. The compound is prepared by carrying out a one-step addition reaction on natural product fangchinoline with alkyl or aryl isocyanate in dichloromethane and triethylamine at room temperature, and the yield is high. An antibacterial activity screening test proves that the fangchinoline-carbamate derivative has a relatively good inhibition effect on fusarium graminearum or phomopsis spp., and activity of the fangchinoline-carbamate derivative is superior to or equal to activity of bactericidal pesticide azoxystrobin with a relatively large usage amount.

The invention relates to a purification method of fangchinoline. The method comprises the following specific steps: (1) refluxing or quenching and extracting fangchinoline medical powder by utilizing an alcohol solution, recovering solvents from the extracting solution until no alcohol flavor exists, and acidifying until the solution is acidic; and (2) performing alkaline regulation on the acidic solution, extracting with chloroform, filtering the extract through an ODS (Ozone Depleting Substance) silicagel column by a dry method, eluting with a methanol-water or acetonitrile-water mixed solvent, recovering solvents from the eluent and drying to obtain the fangchinoline. The purification method has the advantages of low energy consumption, low preparation period and high product purity.

The invention provides a method for determining multi-component content of a Jingyaokang capsule. The method comprises the following steps: S1, preparation of a mixed reference substance solution, precisely weighing strychnine, alpha-cypress alkali, periplocin, 11-carbonyl-beta-frankincense acid, curcuma zedoary bicyclo-enone, hydroxysafflor yellow A, naringin, tetrandrine, fangchinoline and beta-ecdysterone respectively, and dissolving the above materials in a solvent to obtain the mixed reference substance solution for later use; s2, preparation of a test solution, taking the Jingyaokang capsule, adding a solvent for extraction, performing filtering, and taking filtrate to obtain the test solution for later use; s3, content determination, precisely sucking the reference substance solution and the test solution respectively, injecting the solutions into a liquid chromatograph, and performing determining to obtain the content. According to the determination method, the contents of tenchemical components such as strychnine, alpha-jacosinine, periplocin, 11-carbonyl-beta-masticinic acid and naringin in the Jingyaokang capsule are determined, and the quality of the Jingyaokang capsule can be better controlled.

The invention relates to a method for separating and determining tetrandrine and fangchinoline. The method comprises the following steps: 1, dissolving a Stephania tetrandra root raw drug or a traditional Chinese medicinal preparation containing the Stephania tetrandra root in methanol, carrying out ultrasonic treatment, and filtering; and 2, carrying out high performance liquid chromatography detection on the filtrate obtained in step 1, wherein the mobile phase is a methanol-water mixed solution with the volume ratio of 65:35, 1g/L of sodium octanesulfonate is added, the pH value is adusted by acetate acid gracial to 3, the flow velocity is 1mL/min, and the sample introduction amount is 10[mu]L. The method adopting methanol dissolving to carry out sample pretreatment has the advantages of simple operation and low cost, the whole chromatography process time is 14min, the lowest detection limits of tetrandrine and fangchinoline are 0.28mg/L and 0.13mg/L respectively, the linear ranges of the tetrandrine and fangchinoline are 125-4000mg/L and 47-1500mg/L respectively, the linear correlation coefficient is good, the recovery rate of the method is greater than 90%, and RSD is less than 10%.