Method for extracting tetrandrine and fangchinoline from radix stephaniae tetrandrae

A technology of antipredyl and antipredyl, applied in the direction of organic chemistry, etc., can solve the problems of low extraction rate of antiprednisone and the generation of a large amount of waste water, so as to save medicinal resources of antiprednis, increase production rate, reduce The effect of production costs

Inactive Publication Date: 2019-03-08
长沙湘资生物科技有限公司
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Problems solved by technology

[0004] Aiming at the problems such as the low extraction rate of the existing anti-epidemic and the generation of a large amount of waste water during the extraction process, the present invention provides a method for extracting

Method used

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Embodiment 1

[0022] Take 100kg of dried Fangzi root and crush it, add water until the water content reaches 35%, add 10L of Bacillus subtilis to ferment for 2 days, then add 12L of yeast to ferment for 3 days, add 200L of 75% ethanol solution to reflux for extraction for 2 hours, filter, and pour into the filter residue Add 200L of 75% ethanol solution to reflux to extract for 2h, filter, combine the two filtrates, and concentrate the filtrate under reduced pressure at 55°C to 20L to obtain a concentrated solution; add 0.1mol / L hydrochloric acid to the concentrated solution to pH2.5, and use 60L butanol Extract to obtain aqueous phase A and butanol extract A; add 0.001mol / L sodium hydroxide solution to butanol extract A to pH 9, stand and separate to obtain 5L solution C (without butanol), use 0.1mol / L L hydrochloric acid to pH 2.5, extracted with 20L butanol to obtain butanol extract B; butanol extract B was concentrated to dryness under reduced pressure at 55°C to obtain 2.48kg of dry pro...

Embodiment 2

[0028] Take 200kg of dried Fangzi roots and crush them, add water until the water content reaches 40%, add 20L of Bacillus subtilis to ferment for 2 days, then add 22L of yeast to ferment for 3 days, add 500L of 75% ethanol solution to reflux for extraction for 2 hours, filter, and pour into the filter residue Add 300L of 75% ethanol solution to reflux to extract for 2h, filter, combine the two filtrates, and concentrate the filtrate under reduced pressure at 55°C to 40L to obtain a concentrated solution; add 0.1mol / L hydrochloric acid to the concentrated solution to pH2.5, and use 120L butanol Extract to obtain aqueous phase A and butanol extract A; add 0.001mol / L sodium hydroxide solution to butanol extract A to pH9, leave to stand and separate to obtain 8L solution C (without butanol), use 0.1mol / L L hydrochloric acid to pH 2.5, extracted with 30L butanol to obtain butanol extract B; butanol extract B was concentrated to dryness under reduced pressure at 50-65°C to obtain 5....

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PUM

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Abstract

The invention provides a method for extracting tetrandrine and fangchinoline from radix stephaniae tetrandrae. The method comprises the following specific steps: pulverizing dry radix stephaniae tetrandrae, adding bacillus subtilis and yeast for fermentation, performing alcohol extraction, filtration and concentration to a concentrated liquid, adding hydrochloric acid to adjust the pH value, and performing butanol extraction to obtain an aqueous phase A and a butanol extracting solution A; adding sodium hydroxide and hydrochloric acid into the butanol extracting solution A to adjust the pH value, and performing butanol extraction, concentration, sodium carbonate solution dissolving, filtration and ethanol crystallization to obtain the tetrandrine; adding sodium hydroxide into the aqueous phase A to adjust the pH value, and performing butanol extraction, concentration, sodium carbonate solution dissolving, filtration and ethanol crystallization to obtain the fangchinoline. The method can effectively increase the yield of alkaloid of the radix stephaniae tetrandrae, reduce the difficulty of wastewater treatment and improve the utilization rate of radix stephaniae tetrandrae resources.

Description

technical field [0001] The invention belongs to the field of natural product extraction, and relates to a method for extracting antihexidine and antihexidine from Fangji. Background technique [0002] Fangji is an Aristolochiaceae plant, and its roots are used as medicine. The roots are cylindrical, somewhat curved, and there are deep transverse grooves in the curved part, 6-15cm long and 1.5-3cm in diameter. The outer skin is light brown or light brownish yellow, and the outer skin is light grayish yellow when scraped off. The quality is solid, not easy to break, the section is uneven, spiny, the cross-section is off-white to yellow-white, powdery, the skin is thicker, the xylem bundles are gray-yellow, arranged radially. Bitter, slightly astringent. It is better to have large pieces, thick skin, and rich powder. Mainly produced in Hanzhong, Shaanxi. In addition, Hubei, Hunan, Sichuan, Yunnan and other places. It can be harvested in all seasons, but it is better to use...

Claims

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Application Information

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IPC IPC(8): C07D491/18
CPCC07D491/18
Inventor 向华
Owner 长沙湘资生物科技有限公司
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