Method for simultaneously measuring contents of tetrandrine and fangchinoline in medicinal material of root of fangji

Abstract

The invention provides a method for simultaneously measuring contents of tetrandrine and fangchinoline in the medicinal material of the root of fangji. The method comprises the following steps: step A, preparing an extracting solution; to be specific, smashing the root of fangji, weighing a proper amount of powder, extracting 2% of hydrochloric acid methanol, carrying out room temperature ultrasound processing, carrying out preliminary filtering by using a piece of medium-speed filter paper, taking filter liquor and carrying out filtering by using a microporous filter membrane to obtain an extracting solution using tetrandrine and fangchinoline as reference substances; step B, adjusting detection conditions of a high performance liquid chromatograph under conditions of having the chromatographic column of Agilent ZORBAX Extend-C18 (4.68250 mm, 5 microns), the mobile phases of acetonitrile and 0.08% of triethylamine water at a ratio of 75 to 25, an isocratic elution mode, a sampling size of 8 microlitres, a flowing speed of 1.0mL.min-1, a column temperature of 25 DEG C, and a detection wavelength of 282nm; and step C, carrying out feeding, waiting for test sample and reference substance results, and calculating contents of tetrandrine and fangchinoline in all test samples respectively according to an external standard method for calculation with a peak area. After long-term repeated verification, the method is verified to have advantages of high accuracy, precision, and specificity, and high durability of the chromatographic condition.

Description

technical field [0001] The invention belongs to the field of content determination methods of traditional Chinese medicines, and in particular relates to a method for simultaneously determining the contents of tetrandrine and fangchinoline in Fangji medicinal materials. Background technique [0002] Fangji, the dry root of Fangji plant powder Fangji Stephania tetrandra S. Moore, has the effects of diuresis, swelling, dispelling wind and relieving pain. Tetrandrine and fangchinoline are the main chemical constituents of the herbal medicine Fangji. Studies have shown that tetrandrine has various pharmacological effects such as anti-pneumoconiosis, anti-cancer, anti-rheumatoid arthritis, anti-tumor, and anti-multidrug resistance. Fangchinoline has significant biological activities in anti-tumor, anti-cancer, and inhibition of hypertrophic scars. At present, there is a serious problem in the traditional Chinese medicine market of Fangji, that is, the quality of the circulating...

AI Technical Summary

Problems solved by technology

At present, there is a serious problem in the traditional Chinese medicine market of Fangji, that is, the quality of the circulating Fangji medicinal materials is uneven, and the level of content may directly affect the quality and curative effect of Fangji medicinal materials. Establishing a quantitative analysis method for the main components of Fangji Method for testing the quality of medicinal materials

[0003] The 2015 edition of "Chinese Pharmacopoeia" provides a method for the determination of the content of tetrandrine and fangchinoline in Fangji medicinal materials, but the mobile phase of this method contains sodium dodecylsulfonate, which has a lot of foaming, takes a long time to eliminate foam, and dissolves Strict temperature requirements and complicated operation

In view of the defects in the method of "Chinese Pharmacopoeia", many people have carried out improved tests on the mobile phase, but the methods reported in different literatures are different, and all have the defect of complex mobile phase