Stephania hainanensis total alkaloid extract and preparation method thereof
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A technology for total alkaloids in Hainan intolerant
Inactive Publication Date: 2012-09-19
朱毅
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[0005] The above preparation methods all involve ethanol reflux extraction, and tetrahydropalmatine, the active ingredient of the alkaloid in the ground, is easily affected by light and temperature, and dehydrogenated into palmatine with stable structure. The heating and reflux process may cause its loss, resulting in decre
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Embodiment 1
[0053] Take the tuberous roots of Dictyostelium chinensis, cut into pieces, air-dried or dry at low temperature in a cool place, pulverize, add 10 times the concentration of 1% hydrochloric acid solution to soak for 24 hours, ultrasonically extract 4 times for 30 minutes each time, filter the extracts and combine them directly. Super acidic gel type cation exchange resin column, flow rate 1ml / min, eluted with water until there is no polysaccharide reaction, and then eluted with 8% ammonia water ethanol, collect the eluate, and combine the eluate under reduced pressure to recover the solvent to dryness , that is, the total alkaloid extract of Hainan is not tolerated.
Embodiment 2
[0055] Take the tuberous root of Hainan Dizhibu, sliced, air-dried or low-temperature drying in a cool place, pulverized, added 8 times the concentration of 0.5% hydrochloric acid solution to soak for 48 hours, ultrasonically extracted 3 times, 45 minutes each time, the extracts were filtered and combined, the same as After diluting 1 times the concentration of acid aqueous solution, the super acidic macroporous cation exchange resin column, the flow rate is 2ml / min, the water is eluted until there is no polysaccharide reaction, and then eluted with 6% ammonia water ethanol, and the eluate is collected and combined. The eluent is decompressed and the solvent is recovered to dryness to obtain the total alkaloid extract from Hainan.
Embodiment 3
[0057] Take the tuberous roots of Hainan Dizhibu, sliced, air-dried or low-temperature drying in a cool place, pulverized, added 12 times the concentration of 0.5% sulfuric acid solution to soak for 24 hours, ultrasonically extracted twice, 30 minutes each time, the extracts were filtered and combined, and the same After diluting 2 times the concentration of acid aqueous solution, it is super acidic gel type cation exchange resin column, the flow rate is 0.5ml / min, water is eluted until there is no polysaccharide reaction, and then eluted with 5% ammonia water ethanol, the eluate is collected and combined. The eluate was decompressed and the solvent was recovered to dryness to obtain the total alkaloid extract of Hainan intolerant.
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Abstract
The invention belongs to the technical field of pharmacy, and particularly discloses a stephania hainanensis total alkaloid extract extracted from stephania hainanensis tubers and a preparation method thereof. The preparation process is as follows: material (stephania hainanensis dry tubers); grinding; soaking in a moderate amount of 0.01 to 10 percent of hydrochloric acid or sulfuric acid; 1 to 4 times of ultrasonic extraction; filtration; storng-acid cation exchange resin purification; solvent recovery for drying; and stephania hainanensis total alkaloid extract. The extract mainly comprises L-tetrahydropalmatine, palmatine, fangchinoline, hanfangchin and other active ingredients, the percentage by weight of stephania hainanensis total alkaloid is greater than or equal to 70 percent, the percentage by weight of the palmatine is greater than or equal to 20 percent, and the percentage by weight of the fangchinoline is greater than or equal to 40 percent. Reports on the stephania hainanensis total alkaloid extraction process are not found in related literatures so far, the operation flow of the method is simple, the purity of the product is high, the efficacy is remarkable, and the method disclosed by the invention is applicable to industrial production and the exploitation and utilization of local stephania hainanensis resources.
Description
technical field [0001] The invention belongs to the technical field of pharmacy, and in particular relates to an extract of total alkaloids from Hainan intolerant and a preparation method thereof. Background technique [0002] Stephania hainanensis H.S.Lo et Y.Tsoong (Stephania hainanensis H.S.Lo et Y.Tsoong) belongs to the subgenus of the genus Phychiaceae. Also known as Jinbuhuan, mountain turtle, and one penny, its roots are used as medicine, with a bitter taste, cold in nature, and a small poison. It has the functions of clearing away heat and detoxifying, cutting malaria, and analgesia. According to the existing research results, the alkaloid components in Hainan are not allowed to contain cbanine, dehydroxybanine, oxo-cbanine, tetrahydropalmatine, palmatine, 1-cordy monophenolic alkaloid, isosin Culyidine, d-stiphenamine, homoamorinine, tetrandrine, fenugreek, sinomenine, fangchinoline, etc., its main active ingredient fangchinoline has Anti-inflammatory, analgesic,...
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