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Application of fangji root extract and fangchinoline in pharmacy

A technology of fangchinoline base and extract, which is applied in the field of medicine and can solve problems such as no relevant reports

Active Publication Date: 2019-08-30
HKUST SHENZHEN RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Fangji, the main active ingredient in Fangji Dihuang Decoction for the treatment of vascular dementia, is also an alkaloid compound, but there is no relevant report on the inhibitory activity of Fangji and its active ingredients on acetylcholinesterase

Method used

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  • Application of fangji root extract and fangchinoline in pharmacy
  • Application of fangji root extract and fangchinoline in pharmacy
  • Application of fangji root extract and fangchinoline in pharmacy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1 Study on Anti-acetylcholinesterase Activity of Fangji, Coptis Rhizoma Extracts and Their Composition

[0040] 1 Materials and reagents

[0041] 1.1 Drugs and reagents

[0042] Fangji extract and Coptis chinensis extract are self-made in the laboratory (take a single medicinal material, decoct and extract twice, each time with 10 times the amount of water, combine the extracts, concentrate to dryness, and obtain the medicinal material extract).

[0043] DTNB (Lot 16262), acetylcholine iodide (ATCh, Lot BCBV4822), and tetraisopropyl pyrophosphoimide (iso-OMPA, Lot BCBW4257) were purchased from Sigma-Aldrich (USA); NP-40 (Lot C10297258) , Leupeptin (Lot C10079991), Aprotinin (Lot C10060365), Pepsin (Lot B10084382), 4-Hydroxyethylpiperazineethanesulfonic acid (HEPES, Lot B10094032), Benzamidine Hydrochloride (Lot C10089171) and other analytical reagents were purchased from Shanghai Macklin Biochemical Technology Co., Ltd.

[0044] 1.2 Preparation of animal brai...

Embodiment 2

[0065] Example 2 Study on Anti-acetylcholinesterase Activity of Fangji, Phellodendron Phellodendri Extract and Their Composition

[0066] 1 Materials and reagents

[0067] 1.1 Drugs and reagents

[0068] Fangji extract and Coptis chinensis extract are self-made in the laboratory (take a single medicinal material, decoct and extract twice, each time with 10 times the amount of water, combine the extracts, concentrate to dryness, and obtain the medicinal material extract).

[0069] All the other reagents are the same as item 1.1 medicines and reagents in Example 1.

[0070]1.2 Preparation of animal brain lysate

[0071] With the 1.2 item method in embodiment 1.

[0072] 2 Experimental methods

[0073] 2.1 Drug preparation

[0074] Take an appropriate amount of Fangji extract, dissolve it in DMSO to 83.6 mg / mL, and serially dilute it into 7 gradients from the initial concentration of 2 times. Take an appropriate amount of Cortex Phellodendri extract, dissolve it in DMSO to ...

Embodiment 3

[0090] Example 3 Study on anti-acetylcholinesterase activity of fangchinoline, coptisine and their combination

[0091] 1 Materials and reagents

[0092] 1.1 Drugs and reagents

[0093] Fangchinoline (Lot S090409-10, purity 99%) and coptisine hydrochloride (Lot DST170711-003, purity 99%) were purchased from Chengdu Master Biotechnology Co., Ltd.

[0094] All the other reagents are the same as item 1.1 in Example 1.

[0095] 1.2 Preparation of animal brain lysate

[0096] With the 1.2 item method in embodiment 1.

[0097] 2 Experimental methods

[0098] 2.1 Drug preparation

[0099] Precisely weigh appropriate amount of reference substances of fangchinoline and coptisine hydrochloride, and dissolve them in DMSO to 50mM and 25mM respectively.

[0100] Take an appropriate amount of fangchinoline base mother solution, dilute it with DMSO, and serially dilute it into 7 gradients from a 2-fold gradient from the initial concentration of 0.4mM. Take an appropriate amount of cop...

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Abstract

The invention provides application of fangji root extract and fangchinoline in pharmacy. Fangji root extract and fangchinoline are used as components of an acetylcholinesterase inhibitor, and the acetylcholinesterase inhibitor is used for preparing a medicine for preventing and treating Alzheimer disease. The invention provides the application of fangji root extract and fangchinoline in pharmacy,provides a new drug candidate source for preventing and treating Alzheimer disease, and has important significance in the aspects of improving the clinical treatment effect of the Alzheimer disease and reducing the toxic and side effects of drugs.

Description

technical field [0001] The present invention relates to the field of medical technology, in particular to the new use of Fangji Nuoline extract and its functional component Fangchinoline in anti-acetylcholinesterase activity, and their enhanced acetylcholinesterase inhibitory activity after being compatible with other traditional Chinese medicine extracts or functional components It is used in the preparation of prevention and treatment drugs for Alzheimer's disease. Background technique [0002] Alzheimer's disease (AD) is a common degenerative disease of the central nervous system. Its main clinical features are cognitive dysfunction or accompanied by language and activity decline. It has the characteristics of insidious onset and rapid disease progression. patient's quality of life. Due to factors such as changes in lifestyle, increased work pressure, and population aging, the incidence of AD in my country is increasing year by year. The pathogenesis of AD is complex. E...

Claims

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Application Information

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IPC IPC(8): A61K36/59A61K36/718A61K36/756A61K31/4748A61K31/4375A61K31/445A61P25/28
CPCA61K31/4375A61K31/445A61K31/4748A61K36/59A61K36/718A61K36/756A61K2236/331A61K2236/51A61P25/28A61K2300/00
Inventor 詹华强董婷霞孔祥鹏王怀友陈志从任海琴刘韵乐熊清平黄荣蕾
Owner HKUST SHENZHEN RES INST
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