4 results about "Benzamidine" patented technology

This invention discloses an ultrastable hydrogen-bonded organic framework material, its preparation method, and its applications. The preparation method involves mixing a deprotonated solution of 6,6',6'',6'''-(pyrene-1,3,6,8-tetramethyl)tetra(2-naphthoic acid) with a solution of 4,4',4'',4'''-methanetetraphenylamidine tetrahydrochloride. After standing in the dark, the mixture is centrifuged, washed, and lyophilized. The resulting hydrogen-bonded organic framework material exhibits excellent stability and high photocatalytic efficiency, making it effective for the photocatalytic preparation of BHMF from HMF. Adding MsAcT enzyme powder to the 4,4',4'',4'''-methanetetraphenylamidine tetrahydrochloride solution yields a hydrogen-bonded organic framework material encapsulating MsAcT enzyme. This material can be effectively used for the one-pot photocatalytic preparation of BHMF diacetate from HMF.

The present application relates to the preparation method of 3-phenyl-5-aryl-1,2,4-oxadiazole by electro-oxidation, and the preparation reaction is as follows: wherein R is selected from hydrogen, 4-fluoro, 4-chloro, 2-bromo, 4-tert-butyl, 3-methyl or 3,4-dimethyl; Ar is selected from phenyl, 2-thiophenyl, 2-fluorophenyl, 4-chlorophenyl, 2-bromophenyl, 4-fluorophenyl or 4-methylphenyl. The preparation method of electro-oxidation is that (Z)-N'-hydroxy-N-(arylmethyl) benzamidine, organic solvent, base and electrolyte are used as electrolyte in a non-membrane electrolytic cell, and then constant voltage electrolysis is carried out at a certain temperature for a certain time, so that 3-phenyl-5-aryl-1,2,4-oxadiazole (I) is obtained by electro-oxidation reaction. The preparation method of electro-oxidation can be used for preparing line thiophene and ataluren.

The invention discloses a preparation method of an ultraviolet light absorber UV-1600 intermediate, and belongs to the field of fine chemical engineering. The method comprises the following steps: by taking 4-cyanobiphenyl as a raw material, generating 4-phenylbenzamidine under the action of NaHMDS; then carrying out cyclization reaction with 2, 4-dioxo-substituted acetophenone in the presence of elemental iodine; then, a substituent group on the phenol is removed, and the UV-1600 intermediate is generated. According to the method, 4-cyanobiphenyl is adopted to generate biphenyl amidine under the condition of NaHMDS, biphenyl amidine directly reacts with 2, 4-dioxo substituent acetophenone without purification to generate the triazine ring intermediate, the reaction yield is high, operation is easy and convenient, economic benefits are good, and industrial production is easy.

The application relates to a single-cell proteome sample processing method, which comprises the following steps: taking a single-cell sample, adding 0.01-0.2% non-ionic surfactant for ultrasonic lysis to obtain a pretreated sample; performing denaturation reduction alkylation treatment on the pretreated sample to obtain an alkylated sample; adding an immobilized enzyme reactor to the alkylated sample for enzymolysis for 0.5-4 hours, centrifuging to obtain supernatant, and obtaining a processed sample; and a preparation method of the immobilized enzyme reactor, which comprises the following steps: adding polyethyleneimine to amino polymer microspheres to obtain hydrophilic amino polymer microspheres after reaction; adding graphene oxide to the hydrophilic amino polymer microspheres to obtain graphene oxide-polymer microspheres after reaction; adding a protease solution containing benzalkonium to the graphene oxide-polymer microspheres to obtain the immobilized enzyme reactor after reaction.