7 results about "Benzamidine" patented technology

The invention discloses an efficient purification process of a bispecific antibody and a special chromatography system thereof. The efficient purification process of the bispecific antibody comprises the following steps: pretreatment: performing 0.22 mu m microfiltration on a cell culture supernatant to remove cell debris, and performing deep filtration to reduce turbidity to be less than or equal to 1NTU so as to reduce subsequent chromatography medium pollution; carrying out improved Protein A affinity chromatography, namely carrying out affinity chromatography by adopting an agarose medium modified by a recombinant Protein A ligand, taking 20mM Tris-HCl as a loading buffer solution, and taking the pH value of the loading buffer solution as 7.4, taking 100mM citric acid as an elution buffer solution, and taking the pH value of the elution buffer solution as 3.2-3.5; performing mixed mode chromatography: adopting a composite mode medium containing a benzamidine functional group, taking 50mM sodium phosphate as a buffer solution, adjusting the pH value to 6.0-6.5, adjusting the NaCl concentration to 0.1-0.3 M, and specifically removing a double-antibody polymer and a mismatched isomer by utilizing a hydrophobic effect and an ion exchange synergistic effect; and performing nanoscale filtration and terminal treatment: removing viruses by adopting a virus filtration membrane with the aperture of 30nm, and performing 0.22 mu m sterile filtration to obtain a final product. According to the process, the purification efficiency, the yield and the product purity are remarkably improved, and the process is suitable for efficient purification of the bispecific antibody.

The invention provides a composite quality control product of an AIDS antibody, a syphilis antibody and a hepatitis C antibody as well as a preparation method and application of the composite quality control product. The composite quality control product comprises an AIDS (Acquired Immune Deficiency Syndrome) antibody, a syphilis antibody, a hepatitis C antibody and the following components: a matrix solution, 1.5 to 3 g / L of Pluronic F68, 0.05 to 0.15% v / v of ProClin 300, 0.1 to 1 g / L of sodium surfactin, 1 to 10 g / L of bovine serum albumin, 1 g / L of alanine, 2 to 5 g / L of trehalose, 0.5 to 1.0 g / L of EDTA (Ethylene Diamine Tetraacetic Acid) and 2 to 5 mmol / L of benzamidine. The matrix liquid comprises a buffer solution and 20-30% v / v human serum. According to the invention, the aggregation and inactivation risk of the antibody in the freezing and thawing process is effectively reduced, the antibody can be repeatedly frozen and thawed for more than three times, the degradation of the antibody at high temperature can be effectively prevented, and the reliability of the quality control product in the transportation and clinical use process is ensured.

This invention discloses an ultrastable hydrogen-bonded organic framework material, its preparation method, and its applications. The preparation method involves mixing a deprotonated solution of 6,6',6'',6'''-(pyrene-1,3,6,8-tetramethyl)tetra(2-naphthoic acid) with a solution of 4,4',4'',4'''-methanetetraphenylamidine tetrahydrochloride. After standing in the dark, the mixture is centrifuged, washed, and lyophilized. The resulting hydrogen-bonded organic framework material exhibits excellent stability and high photocatalytic efficiency, making it effective for the photocatalytic preparation of BHMF from HMF. Adding MsAcT enzyme powder to the 4,4',4'',4'''-methanetetraphenylamidine tetrahydrochloride solution yields a hydrogen-bonded organic framework material encapsulating MsAcT enzyme. This material can be effectively used for the one-pot photocatalytic preparation of BHMF diacetate from HMF.

The present application relates to the preparation method of 3-phenyl-5-aryl-1,2,4-oxadiazole by electro-oxidation, and the preparation reaction is as follows: wherein R is selected from hydrogen, 4-fluoro, 4-chloro, 2-bromo, 4-tert-butyl, 3-methyl or 3,4-dimethyl; Ar is selected from phenyl, 2-thiophenyl, 2-fluorophenyl, 4-chlorophenyl, 2-bromophenyl, 4-fluorophenyl or 4-methylphenyl. The preparation method of electro-oxidation is that (Z)-N'-hydroxy-N-(arylmethyl) benzamidine, organic solvent, base and electrolyte are used as electrolyte in a non-membrane electrolytic cell, and then constant voltage electrolysis is carried out at a certain temperature for a certain time, so that 3-phenyl-5-aryl-1,2,4-oxadiazole (I) is obtained by electro-oxidation reaction. The preparation method of electro-oxidation can be used for preparing line thiophene and ataluren.

The application provides a complex quality control product of HIV antibody, syphilis antibody and hepatitis C antibody and a preparation method and application thereof. The complex quality control product comprises HIV antibody, syphilis antibody and hepatitis C antibody and the following components: a matrix liquid, 1.5-3 g / L Pluronic F68, 0.05-0.15 % v / v ProClin 300, 0.1-1 g / L sodium mycobacterial lipopeptide, 1-10 g / L bovine serum albumin, 1 g / L alanine, 2-5 g / L trehalose, 0.5-1.0 g / L EDTA and 2-5 mmol / L benzamidine; the matrix liquid comprises a buffer and 20-30 % v / v human serum. The application effectively reduces the aggregation and inactivation risk of the antibodies in the freeze-thaw process, can be repeatedly freeze-thawed more than three times, can effectively prevent the degradation of the antibodies under high temperature, and ensures the reliability of the quality control product in the transportation and clinical use process.

The invention discloses a preparation method of an ultraviolet light absorber UV-1600 intermediate, and belongs to the field of fine chemical engineering. The method comprises the following steps: by taking 4-cyanobiphenyl as a raw material, generating 4-phenylbenzamidine under the action of NaHMDS; then carrying out cyclization reaction with 2, 4-dioxo-substituted acetophenone in the presence of elemental iodine; then, a substituent group on the phenol is removed, and the UV-1600 intermediate is generated. According to the method, 4-cyanobiphenyl is adopted to generate biphenyl amidine under the condition of NaHMDS, biphenyl amidine directly reacts with 2, 4-dioxo substituent acetophenone without purification to generate the triazine ring intermediate, the reaction yield is high, operation is easy and convenient, economic benefits are good, and industrial production is easy.

The application relates to a single-cell proteome sample processing method, which comprises the following steps: taking a single-cell sample, adding 0.01-0.2% non-ionic surfactant for ultrasonic lysis to obtain a pretreated sample; performing denaturation reduction alkylation treatment on the pretreated sample to obtain an alkylated sample; adding an immobilized enzyme reactor to the alkylated sample for enzymolysis for 0.5-4 hours, centrifuging to obtain supernatant, and obtaining a processed sample; and a preparation method of the immobilized enzyme reactor, which comprises the following steps: adding polyethyleneimine to amino polymer microspheres to obtain hydrophilic amino polymer microspheres after reaction; adding graphene oxide to the hydrophilic amino polymer microspheres to obtain graphene oxide-polymer microspheres after reaction; adding a protease solution containing benzalkonium to the graphene oxide-polymer microspheres to obtain the immobilized enzyme reactor after reaction.