61 results about "Triple quadrupole mass spectrometer" patented technology

The invention relates to a traditional Chinese medicine and metabolite identification method based on double mass spectrum platform collaboration, which comprises the following steps: respectively collecting by adopting a liquid chromatogram-triple quadrupole mass spectrometer and a liquid chromatogram-quadrupole time-of-flight mass spectrometer, and simultaneously establishing a corresponding compound database and a qualitative scheme; the LC-MS / MS traditional Chinese medicine component identification method based on double mass spectrum platform collaboration is realized. Chemical components in a traditional Chinese medicine sample are identified through a high-coverage metabolite database, and data are integrated and processed through a grading credibility judgment method, so that high-throughput, high-coverage and high-accuracy traditional Chinese medicine component identification is realized. The method can be applied to single traditional Chinese medicine ingredient identification, compound traditional Chinese medicine ingredient analysis and traditional Chinese medicine related metabonomics analysis and identification, and a basis is provided for further research on traditional Chinese medicine active ingredients and clinical application thereof.

The invention discloses a method for accurately measuring trace calcium in high-purity magnesium metal by combining membrane desolvation and triple quadrupole mass spectrometry, which comprises the following steps: (1) sequentially adding water to wet a high-purity magnesium sample and adding acid to dissolve the sample to obtain a digestion solution, and adding an internal standard solution into the digestion solution; (2) preparing calcium element solutions with multiple concentration gradients, adding the internal standard solution, fixing the volume, and shaking uniformly; (3) sequentially carrying out atomization treatment and membrane desolvation treatment on the prepared solution to respectively obtain dry aerosol of the to-be-detected solution and the standard solution; and (4) carrying out scanning determination on the dry aerosol of the solution to be detected and the standard solution in the step (3) by using a triple quadrupole mass spectrometer, and calculating to obtain the calcium content in the high-purity magnesium sample. According to the method, the 24Mg16O polyatomic interference brought by the matrix element and the mass spectrum interference of the working gas Ar on the to-be-detected element 40Ca are removed through membrane desolvation and triple quadrupole mass spectrometry, so that the trace calcium element in the high-purity magnesium sample can be accurately determined, and the lower determination limit can reach ppb level.

The invention discloses a method for detecting multiple perfluorinated compounds in a water environment. The method comprises the steps of sample collection and preservation, sample pretreatment and LC-MS / MS detection. According to the invention, trace detection is carried out on common perfluorinated compounds in a water environment by adopting solid-phase extraction-liquid chromatography-electrospray-tandem triple quadrupole mass spectrometer, a more perfect and comprehensive LC-MS / MS detection method for multi-medium concentration of perfluorinated compounds is provided, and on the premise that the recovery rate and the accuracy of a detection result are ensured, the detection efficiency is improved. The sample pretreatment modes in different media are optimized and adjusted, so that the experiment process is more convenient and faster, time and labor are saved, the use of traditional glass material consumables is avoided, and the experiment error is reduced; meanwhile, the method disclosed by the invention is wider in application range, can meet trace detection of perfluorinated compounds in surface water, underground water, domestic sewage, industrial wastewater, river sediment and residual sludge of a sewage plant, and provides data support for quality supervision and risk early warning in a water environment.

The invention relates to a marker combination for identifying the year of ham and application of the marker combination, and belongs to the field of food identification, firstly, the content of flavor compounds is detected through a gas chromatography-triple quadrupole mass spectrometer, and the combination capable of being used as an identification marker is obtained through two rounds of screening, so that the year of Jinhua ham is identified; moreover, key discriminant compound data can be screened and simplified, and a set of equipment and algorithm for realizing the model are provided.

A method for obtaining the detection limit of 23 perfluorinated compounds in water relates to the technical field of pollutant detection, and comprises the following steps: step S1, taking a sufficient amount of a water sample and placing the water sample in a fluoride-free volumetric flask to obtain a water sample containing methanol; s2, preparing a water quality sample to be detected; s3, detecting the water quality sample to be detected by adopting a high performance liquid chromatography tandem mass spectrometer; s4, setting an elution program of a mobile phase; and S5, testing, and calculating the concentrations of 23 perfluorinated compounds in the water quality sample to be detected. According to the method, the detection limits of 23 perfluorinated compounds in water are obtained by utilizing a liquid chromatography-triple quadrupole mass spectrometer and adopting an online solid-phase extraction method, so that the detection efficiency is improved, the personal error is reduced, and the consumption of an organic solvent and the use of a traditional solid-phase extraction column are reduced.

The invention discloses a method for simultaneously determining N-sodium laureth sarcosinate and phenol red residues in an injection based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. According to the method, on the basis of an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer, a 5mM ammonium acetate solution (containing 10% acetonitrile) is taken as a phase A, a 0.2% acetic acid acetonitrile solution is taken as a phase B, a Waters Acquity UPLCBEH C18 column is selected, ESI is taken as a mass spectrometry detection ion source, and the two process residues in the injection are accurately quantified at the same time in a negative ion scanning and multi-reaction monitoring mode. The method is simple in pretreatment, high in detection sensitivity and good in reproducibility, and can be used for corresponding injection process residue identification and quality control.

An abnormal site identification method according to the present disclosure is an abnormal site identification method for a triple quadrupole mass spectrometer comprising a first quadrupole and a second quadrupole on opposite sides of a collision cell. The abnormal site identification method includes: a step (S20) of acquiring comparison data; a step (S30) of acquiring reference data; a step (S50) of identifying an abnormal site in the triple quadrupole mass spectrometer on the basis of the reference data and the comparison data; and a step (S60) of reporting the abnormal site.

The invention discloses a method for simultaneously detecting polychloroprene and polychlorobenzene in a biological sample, which comprises the following steps: extracting the biological sample through ultrasonic extraction, replacing the existing rotary evaporation concentration with rapid evaporation concentration, and combining dynamic multi-reaction monitoring of a gas chromatograph-triple quadrupole mass spectrometer to detect the polychloroprene and polychlorobenzene in the biological sample. Synchronous extraction, impurity removal, purification and quantitative and qualitative analysis of 9 polychlorobutadiene and 11 polychlorobenzene in a biological sample can be realized, and the detection efficiency is improved.

The invention provides a full-automatic detection device and method for the concentration of free drugs in blood. The full-automatic detection method adopts a full-automatic detection device and comprises the following steps: (1) loading a blood sample to a protein purification chromatographic column by adopting a pump 1 and an automatic sampler, removing protein components in the blood sample, and retaining free drug components on the protein purification chromatographic column; (2) enabling free drug components in the protein purification chromatographic column to flow into a trapping column and enriching the free drug components in the trapping column; and (3) enabling the free drug components in the trapping column to flow into an analytical column, separating, and detecting in a triple quadrupole mass spectrometer. Through the specific full-automatic detection device, the problems that total drug concentration monitoring cannot meet clinical treatment requirements, and the free drug pretreatment process is complex, long in time and high in cost are solved, clinical individualized drug administration can be assisted, the drug curative effect is improved, and the toxic and side effects of drugs are reduced.

The application provides a method for simultaneously detecting the content of 16 new types of brominated flame retardants in human serum, and the specific steps are as follows: S1: preparing a mixed standard solution; S2: preparing an internal standard solution; S3: drawing a standard working curve; S4: sample pretreatment: taking dichloromethane in a glass test tube, adding the internal standard solution, and blowing with nitrogen in a 40 DEG C water bath until nearly dry, then adding fetal bovine serum and pure water, and vortexing to mix; the mixed solution of dichloromethane and acetonitrile is used to form an emulsion with the sample, and after sufficient extraction of the emulsion by using a dispersing agent and an extracting agent, centrifugation is carried out at room temperature; finally, the organic precipitate phase is taken and filtered through a filter membrane to be transferred to a sample injection vial with a glass lining tube, and is left for machine detection; S5: detection and result analysis: a gas chromatograph-tandem triple quadrupole mass spectrometer is used for detecting and analyzing the content of the new types of brominated flame retardants. The method has the advantages of simple pretreatment, high sensitivity and strong practicability, and can be used for routine biological monitoring of NBFRs in the human population.

The invention discloses a method for simultaneously determining the contents of baohuoside, mangiferin, kaempferol, timosaponin A III and timosaponin B II in rhizoma anemarrhenae based on a UPLC-MS / MS (Ultra Performance Liquid Chromatography-Mass Spectrometry / Mass Spectrometry) method. The method is based on an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer, a methanol organic phase-0.1% formic acid water phase system is used as a mobile phase, a Waters Acquity UPLCBEH C18 column is selected, ESI is used as a mass spectrometry detection ion source, and the contents of the five traditional Chinese medicines in the rhizoma anemarrhenae powder are accurately quantified at the same time in a positive and negative ion synchronous scanning and multi-reaction monitoring mode. The method has the advantages of simple pretreatment, high detection sensitivity and good reproducibility, and can be used for identification or quality control of main components of corresponding traditional Chinese medicines.

The present invention relates to a method for determining the content of COX-2 metabolites in the arachidonic acid metabolic pathway in an inflammation model, comprising the following steps: (1) Pretreating the sample: Pretreating the cell supernatant sample and / or serum sample, and adding an internal standard working solution during the pretreatment process, wherein the internal standard working solution is a PGA2-D4 solution; (2) Detecting the pretreated sample using an Agilent 6495 triple quadrupole mass spectrometer. The method provided by the present invention can simultaneously detect the content of 11 metabolites in the AA metabolic pathway, and has the advantages of rapidity, high sensitivity, good stability, etc.

The application discloses a method for simultaneously detecting 32 kinds of veterinary drug residues in fecal pollution, and belongs to the technical field of veterinary drug residue detection. The method comprises the following steps: taking a fecal pollution sample, adding Na2EDTA-Mcllvaine buffer solution and acetonitrile for extraction, centrifuging the sample after salting-out to obtain an organic phase, purifying the organic phase through an LPAS purification column, collecting the purified liquid, concentrating and blowing dry, redissolving, filtering, and obtaining a to-be-detected liquid; detecting the to-be-detected liquid by using an ultra-high performance liquid chromatography-triple quadrupole mass spectrometer, gradient elution; and quantitatively detecting 32 kinds of veterinary drugs by using a matrix matching standard curve method. The method is simple in operation and good in purification effect, can effectively remove interfering substances such as fat and protein in the fecal pollution, significantly reduces the matrix effect, is suitable for simultaneously, quickly and accurately detecting 23 kinds of sulfonamides and 9 kinds of fluoroquinolones in pig, chicken, cow and duck fecal pollution, and has good accuracy and precision.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

The invention discloses a method for detecting methylation level of heterozygous heterologous DNA (deoxyribonucleic acid) in saccharomyces cerevisiae. Comprising the following steps: preparing a saccharomyces cerevisiae genome, carrying out enzymolysis on the saccharomyces cerevisiae genome, and detecting the methylation level of heterozygous heterologous DNA in saccharomyces cerevisiae by using an ultra-high performance liquid chromatography-triple quadrupole mass spectrometer. According to the method, saccharomyces cerevisiae cell walls can be better removed, the use time of protease K is adjusted, protein combined with DNA molecules is better removed, saccharomyces cerevisiae genomes are extracted with high quality, and impurities are greatly reduced; the PMSF protease inhibitor is added in the saccharomyces cerevisiae genome extraction process, so that the activity of protease K can be effectively inhibited, the residual protease K is prevented from degrading nuclease and other key enzymes in the subsequent DNA enzymolysis process, and the DNA enzymolysis efficiency is improved. The method can be directly applied to DNA methylation detection of saccharomyces cerevisiae.

1. The name of the design product: full-automatic liquid chromatography tandem mass spectrometry detector (Automs Q600). 2. The use of the design product: for processing samples and performing quantitative and qualitative analysis of samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: combined state diagram. 5. Other circumstances that need to be explained: kit 1 is a sample pretreatment system; kit 2 is a liquid chromatograph; kit 3 is a triple quadrupole mass spectrometer.

The invention discloses a method for synchronously detecting quaternary ammonium salts with different polarities and structural analogues thereof in high-organic-matter soil / sediment. The method comprises the following steps: performing freeze drying and low-temperature homogeneous grinding on a soil or sediment matrix sample, adding an internal standard substance and an extracting agent, performing vortex mixing, performing ultrasonic extraction, and performing low-temperature and high-speed centrifugation to obtain an extracting solution; heating in a water bath, carrying out nitrogen blowing concentration on an extracting solution until the extracting solution is nearly dry to obtain a concentrated solution, carrying out purification, leaching and impurity removal on the concentrated solution in a methanol-activated solid-phase extraction column, concentrating an eluent, adding a recovery rate indicator, and fixing the volume to obtain a sample to be detected; and detecting the sample to be detected by adopting an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. According to the method, quaternary ammonium salts with different polarities and structural analogues thereof can be accurately quantified, and compared with a traditional method, the pollutant detection class group is wider, the recovery rate of substances to be detected is high, and the stability is higher.

The invention belongs to the technical field of medical analysis, and discloses a pyrrole acyl piperidylamine compound and a method for detecting genotoxic impurities of the pyrrole acyl piperidylamine compound. Specifically, the invention relates to analysis and limit determination of four genotoxic impurities in a pyrroloyl piperidylamine new drug (code name compound 1) for resisting drug-resistant bacterium infection. According to the method, a liquid chromatography-mass spectrometry technology is adopted, octadecyl bonded silica gel is used as a stationary phase, a mixed solution of methanol and an ammonium acetate acidic aqueous solution is used as a mobile phase, and triple quadrupole mass spectrometry is used as a detector for analysis and determination. The genotoxic impurities comprise halogenated alkanes, halogenated olefins, carbamates and azo oxides. The method has relatively good specificity and sensitivity, can be used for accurately and quantitatively determining the genotoxic impurities, and is simple and rapid to operate. The method provides a method basis for quality control of the compound 1, and has important application value for quality control and safety guarantee in a new drug research and development process.

The invention relates to a plant carrier in-situ monitoring method for atmospheric polycyclic aromatic hydrocarbons and derivatives thereof based on usnea spinifera, and belongs to the technical field of monitoring methods. The technical problems that an existing PAHs monitoring method needs to be supported by an environment monitoring station and an atmosphere monitoring sampler, a large amount of labor and cost are consumed, and large-scale pollution monitoring of PAHs and SPAHs in remote mountainous areas is not facilitated are solved. The method comprises the following steps: cleaning, freeze-drying, crushing and sieving usnea spinifera to obtain an usnea spinifera powder sample, extracting, drying and concentrating to obtain an extracting solution; then loading the extract to an activated silica gel column for elution, and carrying out gas pushing on the obtained eluent; and finally, concentrating the gas-pushed liquid, and carrying out gas chromatography-triple quadrupole mass spectrometer detection. According to the method, usnea spinosa is used as an air pollutant accumulation carrier, large-area high-resolution monitoring of PAHs and SPAHs pollution in remote mountainous areas can be completed, and effectiveness and accuracy are high.

The invention provides a method for simultaneously detecting multiple components in a callicarpa nudiflora medicinal material. Specifically, the invention provides the detection method for the callicarpa nudiflora medicinal material based on an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer, the target components detected by the method comprise geniposidic acid, decaffeoyl verbascoside, caffeic acid, cistanche glycoside F, protocatechuic aldehyde, ferulic acid, luteoloside, verbascoside, isoverbascoside, 2-acetyl eugenol glycoside and luteolin. The method disclosed by the invention can be used for accurately, quickly and comprehensively detecting and evaluating the quality of the callicarpa nudiflora medicinal material.

The invention provides a method for simultaneously detecting the content of 16 novel brominated flame retardants in human serum. The method comprises the following specific steps: S1, preparing a mixed standard working solution; s2, preparing an internal standard working solution; s3, drawing a standard working curve; s4, sample pretreatment: adding dichloromethane into a glass test tube, adding an internal standard working solution, carrying out water bath at 40 DEG C, carrying out nitrogen blowing until the solution is nearly dry, adding fetal calf serum and pure water, and carrying out vortex uniform mixing; the method comprises the following steps: absorbing a mixed solution of dichloromethane and acetonitrile and a sample to form an emulsion, carrying out vortex full extraction on the emulsion by using a dispersing agent and an extracting agent, and centrifuging at room temperature; finally, sucking an organic precipitation phase, filtering through a filter membrane, transferring into a small sample introduction bottle with a glass lining tube, and standing for on-machine detection; and S5, detection and result analysis: carrying out detection and result analysis on the content of the novel brominated flame retardant by adopting a gas chromatography-tandem triple quadrupole mass spectrometer. The method disclosed by the invention is simple in pretreatment, high in sensitivity and strong in practicability, and can be used for conventional biological monitoring of the NBFRs of the crowd.

The present invention provides a method for amplifying and converting signals of a triple quadrupole mass spectrometer system. The method analyzes corresponding detected peak signal sub-spectra extracted from analog detection signal spectra output by the triple quadrupole mass spectrometer system at different times to determine whether the analog detection signal currently output by the mass spectrometer system is normal. And when the analog detection signal output by the mass spectrometer system is abnormal, the power supply voltage of the mass spectrometer system is corrected, and then the analog voltage signals included in the corrected analog detection signal spectrum after correcting the power supply voltage are amplified and AD-converted to obtain corresponding analog voltage - digital voltage floating change values, so as to store the digital voltage signals in a differentiated manner. It uses a non-fixed reference voltage signal to amplify and AD-convert the analog voltage signals, thereby reducing the conversion drift deviation of the converted digital voltage signals and improving the accuracy and reliability of mass spectrometer detection.

The invention discloses a nitrosamine impurity analysis method and application thereof, and belongs to the technical field of pharmaceutical analysis. According to the analysis method for the nitrosamine impurities, an LC-MS / MS method is adopted for analysis, and the chromatographic conditions include that ODS C18 serves as a stationary phase, an acidic aqueous solution serves as a mobile phase A, an organic solvent serves as a mobile phase B, and gradient elution is carried out; the mass spectrum conditions comprise that a triple quadrupole mass spectrum detector is adopted, an electrospray ionization positive ion mode is adopted, and multi-reaction monitoring is carried out; the nitrosamine impurity is prepared from N-nitroso-Vonoprazan (N-nitroso-Vonoprazan). The invention also discloses an application of the analysis method, and the analysis method is used for analyzing impurities in the Vonoprazan fumarate or / and the preparation of the Vonoprazan fumarate. The method is scientific in design and ingenious in conception, and the method for analyzing the nitrosamine impurities in the Vonoprasone fumarate and the preparation Vonoprasone fumarate tablets of the Vonoprasone fumarate is provided for the first time; the analysis method has the advantages of strong specificity, high sensitivity, good accuracy and wide durability.

The invention discloses a wine volatile component analysis method based on gas chromatography-tandem mass spectrometry, and aims to improve the detection precision and efficiency of volatile components in wine. According to the method, a two-dimensional gas chromatography unit is connected in series with a triple quadrupole mass spectrometer, and a controller is connected between the two-dimensional gas chromatography unit and the triple quadrupole mass spectrometer for control; in the analysis process, the controller dynamically adjusts the acquisition time window, the starting sequence and the residence time of the triple quadrupole mass spectrometer according to the retention time, the signal-to-noise ratio and the chemical characteristics of the target component, so that accurate acquisition and analysis of different volatile components are realized; according to the method, by optimizing an acquisition strategy, components with relatively high volatility are preferentially acquired, and a reaction channel is activated or closed according to signal intensity, so that the analysis efficiency and accuracy are effectively improved, and the method is suitable for efficient analysis of various volatile components in a complex sample.

The invention discloses a method for determining 14 mycotoxins in peanuts based on high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of food detection. The method comprises the steps of solution preparation, test solution preparation and high performance liquid chromatography-tandem mass spectrometry detection: taking an acetonitrile-water-acetic acid mixed solution as an extracting solution, carrying out grinding, low-temperature ultrasonic extraction, centrifugation, concentration, redissolution and filter membrane filtration on a peanut sample, then separating by adopting a GL Sciences InertSustaiin AQ C18 chromatographic column, and detecting in a multi-stage reaction monitoring mode by adopting a triple quadrupole mass spectrometer. The method provided by the invention realizes synchronous determination of 14 mycotoxins, has the advantages of simple pretreatment, high detection efficiency, good sensitivity and high accuracy, and can be used for accurate detection of mycotoxins in peanuts.

The invention belongs to the technical field of environmental chemical pollutant analysis, and particularly relates to a method for analyzing novel plasticizer pollutants in brain tissues of East Asian Jiangguinea pigs. The method comprises the following steps: carrying out pretreatment on a brain tissue dry sample by adopting ultrasonic extraction and dispersive solid-phase extraction, and then carrying out quantitative analysis by utilizing a gas chromatograph-triple quadrupole mass spectrometer. By optimizing the pretreatment process and instrument detection conditions, matrix interference can be remarkably reduced, and synchronous and accurate quantification of 16 novel plasticizer pollutants is realized. The method has the characteristics of simplicity and convenience in operation, low cost, high sensitivity, good linear relationship and the like, and is suitable for reliable detection of novel plasticizer pollutants in brain tissues of East Asian Jiangguinea pigs.

The invention relates to a method for detecting phenol butyl phenolphthalein drugs in tea-containing weight-losing food, and belongs to the technical field of food detection. The method comprises the following operation steps: (1) pretreatment of a detected object: firstly, treating the detected object into a to-be-purified solution, and filtering by adopting a composite solid-phase extraction column in which a composite filler is composed of florisil, a filler and a mixed strong cation exchange reverse-phase adsorbent; (2) qualitative detection: detecting the to-be-detected solution through a liquid chromatography-tandem triple quadrupole mass spectrometer, and comparing the relative abundance of qualitative ion pairs in the to-be-detected solution with the relative abundance of 15 phenol butyl standard solutions with the same concentration, and if the relative abundance deviation does not exceed the maximum allowable deviation, judging that the phenol butyl phenolphthalein medicine exists in the to-be-detected substance; and (3) quantitative detection: calculating the concentration of a specific phenol butyl phenolphthalein drug in the to-be-detected substance by adopting an external standard method. According to the composite solid-phase extraction column in pretreatment, the baseline can be reduced by one of three parts, the interference of baseline noise can be effectively reduced, and the pretreatment time is shortened.

The invention relates to the technical field of amino acid detection, in particular to a method for detecting amino acid in fresh pears, an experimental instrument and a reagent, sample preparation and chromatographic and mass spectrum conditions. The method utilizes triple quadrupole mass spectrometry, is suitable for determination of low-concentration amino acid, has good selectivity, allows a target substance to have good chromatographic separation and mass spectrometry response, allows a standard curve to be good in a linear range, and can be applied to detection of low-concentration amino acid. The intra-day and inter-day precision, the accuracy, the quantitation limit, the recovery rate and the matrix effect all meet the verification requirements of related chemical methods, and the method is suitable for rapid and simple detection of the amino acids in the pear fruits.

The application provides a multi-component quantitative analysis method of anti-aging tablets, acetonitrile-0.1% formic acid aqueous solution is used as a mobile phase, an HSS T3 chromatographic column is used for target compound separation, and 13 active components in the anti-aging tablets are simultaneously determined in an MRM mode by using an electrospray ionization triple quadrupole mass spectrometer, the linear range of the 13 active components is 0.4-100 ng / mL, the linear range of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf and ginsenoside Rg1 is 4-1000 ng / mL, the method has high sensitivity, strong specificity and good reproducibility, and realizes simultaneous quantitative analysis of the 13 active components in the anti-aging tablets.

The invention belongs to a mass spectrometry technology, and particularly provides a detection method of a triple quadrupole mass spectrometer, which comprises the following steps: A1, setting basic parameters of the mass spectrometer, and establishing compound acquisition initial parameters; the basic parameters comprise detection window time T11 and target detection time T12, and the initial parameters comprise names of compounds, parent ions and channel ion pairs; a2, method parameters are optimized according to the basic parameters and the initial parameters, and the method parameters comprise method time T21, the fragment number M and fragment scanning time T22; and A3, performing ionization, ion screening, collision and detection by the mass spectrometer according to the parameters of the method. The method has the advantages of good accuracy, high throughput and the like.