23 results about "Triple quadrupole mass spectrometer" patented technology

An abnormal site identification method according to the present disclosure is an abnormal site identification method for a triple quadrupole mass spectrometer comprising a first quadrupole and a second quadrupole on opposite sides of a collision cell. The abnormal site identification method includes: a step (S20) of acquiring comparison data; a step (S30) of acquiring reference data; a step (S50) of identifying an abnormal site in the triple quadrupole mass spectrometer on the basis of the reference data and the comparison data; and a step (S60) of reporting the abnormal site.

The invention provides a full-automatic detection device and method for the concentration of free drugs in blood. The full-automatic detection method adopts a full-automatic detection device and comprises the following steps: (1) loading a blood sample to a protein purification chromatographic column by adopting a pump 1 and an automatic sampler, removing protein components in the blood sample, and retaining free drug components on the protein purification chromatographic column; (2) enabling free drug components in the protein purification chromatographic column to flow into a trapping column and enriching the free drug components in the trapping column; and (3) enabling the free drug components in the trapping column to flow into an analytical column, separating, and detecting in a triple quadrupole mass spectrometer. Through the specific full-automatic detection device, the problems that total drug concentration monitoring cannot meet clinical treatment requirements, and the free drug pretreatment process is complex, long in time and high in cost are solved, clinical individualized drug administration can be assisted, the drug curative effect is improved, and the toxic and side effects of drugs are reduced.

The application provides a method for simultaneously detecting the content of 16 new types of brominated flame retardants in human serum, and the specific steps are as follows: S1: preparing a mixed standard solution; S2: preparing an internal standard solution; S3: drawing a standard working curve; S4: sample pretreatment: taking dichloromethane in a glass test tube, adding the internal standard solution, and blowing with nitrogen in a 40 DEG C water bath until nearly dry, then adding fetal bovine serum and pure water, and vortexing to mix; the mixed solution of dichloromethane and acetonitrile is used to form an emulsion with the sample, and after sufficient extraction of the emulsion by using a dispersing agent and an extracting agent, centrifugation is carried out at room temperature; finally, the organic precipitate phase is taken and filtered through a filter membrane to be transferred to a sample injection vial with a glass lining tube, and is left for machine detection; S5: detection and result analysis: a gas chromatograph-tandem triple quadrupole mass spectrometer is used for detecting and analyzing the content of the new types of brominated flame retardants. The method has the advantages of simple pretreatment, high sensitivity and strong practicability, and can be used for routine biological monitoring of NBFRs in the human population.

The application discloses a method for simultaneously detecting 32 kinds of veterinary drug residues in fecal pollution, and belongs to the technical field of veterinary drug residue detection. The method comprises the following steps: taking a fecal pollution sample, adding Na2EDTA-Mcllvaine buffer solution and acetonitrile for extraction, centrifuging the sample after salting-out to obtain an organic phase, purifying the organic phase through an LPAS purification column, collecting the purified liquid, concentrating and blowing dry, redissolving, filtering, and obtaining a to-be-detected liquid; detecting the to-be-detected liquid by using an ultra-high performance liquid chromatography-triple quadrupole mass spectrometer, gradient elution; and quantitatively detecting 32 kinds of veterinary drugs by using a matrix matching standard curve method. The method is simple in operation and good in purification effect, can effectively remove interfering substances such as fat and protein in the fecal pollution, significantly reduces the matrix effect, is suitable for simultaneously, quickly and accurately detecting 23 kinds of sulfonamides and 9 kinds of fluoroquinolones in pig, chicken, cow and duck fecal pollution, and has good accuracy and precision.

The invention relates to the technical field of pharmaceutical analysis, and discloses a method for simultaneously determining the contents of various components in Shuanling antidiarrheal granules based on a UPLC-QQQ-MS / MS. The contents of 18 components in the Shuanling antidiarrheal granules are simultaneously determined by using an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. The 18 components comprise adenosine, gallic acid, protocatechuic acid, neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin, ellagic acid, kaempferitrin, narirutin, hesperidin, baicalin, oroxylin A-7-O-beta-D-glucuronide, wogonin, baicalein, wogonin, pachymic acid B and pachymic acid A. The invention further discloses a preparation method of the pharmaceutical composition. Through the UPLC-QTRAP-MS / MS combined technology, the technical prejudice that single component depends on in quality control of the traditional Chinese medicine compound preparation is broken through, the key problems that multiple components in a complex matrix are difficult to separate, low-content components are difficult to detect and the like are solved, efficient, accurate and synchronous determination of 18 components is achieved, and the method is suitable for large-scale popularization and application. A new reference path is opened up for modernized quality control of the traditional Chinese medicine compound preparation.

1. The name of the design product: full-automatic liquid chromatography tandem mass spectrometry detector (Automs Q600). 2. The use of the design product: for processing samples and performing quantitative and qualitative analysis of samples. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: combined state diagram. 5. Other circumstances that need to be explained: kit 1 is a sample pretreatment system; kit 2 is a liquid chromatograph; kit 3 is a triple quadrupole mass spectrometer.

The invention discloses a method for synchronously detecting quaternary ammonium salts with different polarities and structural analogues thereof in high-organic-matter soil / sediment. The method comprises the following steps: performing freeze drying and low-temperature homogeneous grinding on a soil or sediment matrix sample, adding an internal standard substance and an extracting agent, performing vortex mixing, performing ultrasonic extraction, and performing low-temperature and high-speed centrifugation to obtain an extracting solution; heating in a water bath, carrying out nitrogen blowing concentration on an extracting solution until the extracting solution is nearly dry to obtain a concentrated solution, carrying out purification, leaching and impurity removal on the concentrated solution in a methanol-activated solid-phase extraction column, concentrating an eluent, adding a recovery rate indicator, and fixing the volume to obtain a sample to be detected; and detecting the sample to be detected by adopting an ultra-high performance liquid chromatography-tandem triple quadrupole mass spectrometer. According to the method, quaternary ammonium salts with different polarities and structural analogues thereof can be accurately quantified, and compared with a traditional method, the pollutant detection class group is wider, the recovery rate of substances to be detected is high, and the stability is higher.

The invention relates to a plant carrier in-situ monitoring method for atmospheric polycyclic aromatic hydrocarbons and derivatives thereof based on usnea spinifera, and belongs to the technical field of monitoring methods. The technical problems that an existing PAHs monitoring method needs to be supported by an environment monitoring station and an atmosphere monitoring sampler, a large amount of labor and cost are consumed, and large-scale pollution monitoring of PAHs and SPAHs in remote mountainous areas is not facilitated are solved. The method comprises the following steps: cleaning, freeze-drying, crushing and sieving usnea spinifera to obtain an usnea spinifera powder sample, extracting, drying and concentrating to obtain an extracting solution; then loading the extract to an activated silica gel column for elution, and carrying out gas pushing on the obtained eluent; and finally, concentrating the gas-pushed liquid, and carrying out gas chromatography-triple quadrupole mass spectrometer detection. According to the method, usnea spinosa is used as an air pollutant accumulation carrier, large-area high-resolution monitoring of PAHs and SPAHs pollution in remote mountainous areas can be completed, and effectiveness and accuracy are high.

The invention discloses a wine volatile component analysis method based on gas chromatography-tandem mass spectrometry, and aims to improve the detection precision and efficiency of volatile components in wine. According to the method, a two-dimensional gas chromatography unit is connected in series with a triple quadrupole mass spectrometer, and a controller is connected between the two-dimensional gas chromatography unit and the triple quadrupole mass spectrometer for control; in the analysis process, the controller dynamically adjusts the acquisition time window, the starting sequence and the residence time of the triple quadrupole mass spectrometer according to the retention time, the signal-to-noise ratio and the chemical characteristics of the target component, so that accurate acquisition and analysis of different volatile components are realized; according to the method, by optimizing an acquisition strategy, components with relatively high volatility are preferentially acquired, and a reaction channel is activated or closed according to signal intensity, so that the analysis efficiency and accuracy are effectively improved, and the method is suitable for efficient analysis of various volatile components in a complex sample.

The invention discloses a method for determining 14 mycotoxins in peanuts based on high performance liquid chromatography-tandem mass spectrometry, and belongs to the technical field of food detection. The method comprises the steps of solution preparation, test solution preparation and high performance liquid chromatography-tandem mass spectrometry detection: taking an acetonitrile-water-acetic acid mixed solution as an extracting solution, carrying out grinding, low-temperature ultrasonic extraction, centrifugation, concentration, redissolution and filter membrane filtration on a peanut sample, then separating by adopting a GL Sciences InertSustaiin AQ C18 chromatographic column, and detecting in a multi-stage reaction monitoring mode by adopting a triple quadrupole mass spectrometer. The method provided by the invention realizes synchronous determination of 14 mycotoxins, has the advantages of simple pretreatment, high detection efficiency, good sensitivity and high accuracy, and can be used for accurate detection of mycotoxins in peanuts.

The invention belongs to the technical field of environmental chemical pollutant analysis, and particularly relates to a method for analyzing novel plasticizer pollutants in brain tissues of East Asian Jiangguinea pigs. The method comprises the following steps: carrying out pretreatment on a brain tissue dry sample by adopting ultrasonic extraction and dispersive solid-phase extraction, and then carrying out quantitative analysis by utilizing a gas chromatograph-triple quadrupole mass spectrometer. By optimizing the pretreatment process and instrument detection conditions, matrix interference can be remarkably reduced, and synchronous and accurate quantification of 16 novel plasticizer pollutants is realized. The method has the characteristics of simplicity and convenience in operation, low cost, high sensitivity, good linear relationship and the like, and is suitable for reliable detection of novel plasticizer pollutants in brain tissues of East Asian Jiangguinea pigs.

The application provides a multi-component quantitative analysis method of anti-aging tablets, acetonitrile-0.1% formic acid aqueous solution is used as a mobile phase, an HSS T3 chromatographic column is used for target compound separation, and 13 active components in the anti-aging tablets are simultaneously determined in an MRM mode by using an electrospray ionization triple quadrupole mass spectrometer, the linear range of the 13 active components is 0.4-100 ng / mL, the linear range of ginsenoside Rb1, ginsenoside Rb2, ginsenoside Rc, ginsenoside Rd, ginsenoside Re, ginsenoside Rf and ginsenoside Rg1 is 4-1000 ng / mL, the method has high sensitivity, strong specificity and good reproducibility, and realizes simultaneous quantitative analysis of the 13 active components in the anti-aging tablets.

The invention belongs to a mass spectrometry technology, and particularly provides a detection method of a triple quadrupole mass spectrometer, which comprises the following steps: A1, setting basic parameters of the mass spectrometer, and establishing compound acquisition initial parameters; the basic parameters comprise detection window time T11 and target detection time T12, and the initial parameters comprise names of compounds, parent ions and channel ion pairs; a2, method parameters are optimized according to the basic parameters and the initial parameters, and the method parameters comprise method time T21, the fragment number M and fragment scanning time T22; and A3, performing ionization, ion screening, collision and detection by the mass spectrometer according to the parameters of the method. The method has the advantages of good accuracy, high throughput and the like.

The invention relates to a method for detecting pesticide residues of agricultural products, and solves the bottleneck of poor stability of cross-agricultural-product detection in a rapid agricultural-product pesticide detection process. The method comprises the following steps: constructing a mass spectrum dictionary in a training mass spectrum set range; using the training mass spectrum set to train a mass spectrum recognition model under the guidance of the mass spectrum dictionary to perform pesticide category recognition according to the mass spectrum; pesticide residue detection by applying the mass spectrum recognition model comprises the following steps: scraping a sample from a set sampling position on the surface of a to-be-detected agricultural product by using a sampling probe; the sampling probe after sampling is directly inserted into a thermal desorption electrospray ion source for thermal desorption electrospray ionization, and ionized set charged ions enter a triple quadrupole mass spectrometer for mass spectrometric detection to obtain mass spectrometric data to be detected; and providing to-be-detected mass spectrum data to the mass spectrum identification model for pesticide identification. And designing a mass spectrum dictionary to guide a mass spectrum recognition model to learn pesticide characteristics in a training stage so as to improve the effect of recognizing pesticides according to the rapid pesticide residue detection mass spectrum.

1. The name of the design product: liquid chromatography tandem quadrupole mass spectrometer (An Yi spectrum TQ9120). 2. The use of the design product: for chromatography mass spectrometry qualitative and quantitative analysis of samples such as environmental detection, clinical diagnosis, food safety, life science, multi-omics, etc. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention relates to the technical field of data storage of triple quadrupole mass spectrometers, in particular to a reconfigurable memory hybrid architecture applied to a triple quadrupole mass spectrometer. The system comprises a dynamically reconfigurable hybrid memory controller (supporting parallel / hierarchical mode switching), a heterogeneous memory module (DRAM + NVM), an address mapping table (including valid bits / dirty data bits / heat counting), a memory access monitoring module and a page migration module. By dynamically adjusting a memory architecture mode and combining AXI4-QoS bandwidth control and low power consumption design, the access delay of the mass spectrometer is reduced by 42% (45ns), the static power consumption is reduced to 4.8 mW, and the data error rate is 10-12. The invention is suitable for portable mass spectrum equipment in the fields of environmental monitoring and the like, and has remarkable performance and energy efficiency advantages. The problem that the memory requirements of the mass spectrometer in different working stages cannot be dynamically adapted in the prior art is solved.

The application discloses a kind of detection methods of smell substance in water, comprising the following steps: (1) using solid phase extraction fiber to the water sample in salting-out treatment is carried out adsorption enrichment;(2) by gas chromatography-quadrupole mass spectrometer to the solid phase extraction fiber of adsorption enrichment after step (1) is carried out thermal desorption, and carries out determination, according to the standard curve of smell substance established in advance, the type and content of smell substance in water are obtained by analysis.The application adopts solid phase headspace microextraction to be combined with gas chromatography-quadrupole mass spectrometer method to simultaneously determine a plurality of smell substances rapidly, utilizes pretreatment (extraction, enrichment), improves chromatographic separation efficiency, and utilizes high sensitivity of triple quadrupole mass spectrometer, improves anti-interference ability, effectively reduces interference, while combining salting-out, solid phase headspace microextraction, simplify the cumbersome steps of sample pretreatment, reduce the detection limit of detection method.

The invention discloses a traditional Chinese medicine component indication prediction method in the technical field of medicine prediction, and the method comprises the steps: employing a triple quadrupole mass spectrometer to quantitatively analyze the content of each active component of a traditional Chinese medicine component, and calculating a component weight coefficient; known target spots are collected through a database, potential target spots are predicted through a tool, and a target spot set is formed through normalization duplicate removal; carrying out three-layer progressive screening on the core target spot by virtue of network pharmacology primary screening, molecular docking secondary screening and molecular dynamics verification; through R language enrichment analysis, indication score calculation and determination of high-relevancy potential indications, the technical scheme of the invention improves the target reliability and prediction accuracy, reduces the research and development cost of new traditional Chinese medicines, and is suitable for positioning the indications of 1.2 and 2.3 new traditional Chinese medicines.

The invention relates to a method suitable for detecting three aconitine in various compound traditional Chinese medicine preparations, which comprises the following steps: preparing a reference extract solution and a test solution, introducing a magnetic solid-phase extraction technology when the test solution is prepared, and accurately'capturing 'and separating target aconitine from a complex chemical component background; a multi-reaction monitoring mode of triple quadrupole mass spectrometry is adopted, and a Shim-pack Scept C18-120 chromatographic column is adopted as a chromatographic column; the column temperature is 40 DEG C; a mobile phase is 0.14% formic acid aqueous solution (A)-acetonitrile (B), gradient elution is carried out for 0-5 min, 95% A > 5% A; the flow rate is 0.3 mL * min <-1 >; the sample size is 1 microliter; a mass spectrum ion source is an electrospray ionization source, a positive ion multi-reaction monitoring mode is adopted, the capillary voltage is 3.5 kV, the ion source temperature is 150 DEG C, and the solvent removal temperature is 500 DEG C. The high-efficiency purification of MCX and the high-specificity detection of LC-MS / MS are combined to form a set of powerful method system, the method system is successfully applied to various dosage forms with different formulas, and the industry dilemma of'one preparation and one method 'is solved.

The invention discloses a multi-mode ion source combination device and a detection method thereof.The multi-mode ion source combination device comprises a fixed shell, a heating module, two probe sample introduction modules, an ESI module, a voltage module and a control unit, the two probe sample introduction modules are located above and on the left side of the fixed shell respectively, the heating module is installed above the fixed shell, and the ESI module is installed on the left side of the fixed shell; the ESI module is installed on the left side of the fixed shell, the fixed shell is matched with a triple quadrupole mass spectrometer interface, and the control unit can control equipment to be switched in situ between a TD-ESI mode and a nano-ESI mode. The equipment does not need a solid-liquid conversion process, the detection speed is less than or equal to 1min, a 100-1000Da full molecular weight domain is covered, no sample transfer loss exists, complicated trace material evidences such as explosion residues, drug metabolites and biotoxins can be rapidly detected, the technical problems of difficulty in sampling, narrow range and low efficiency of an existing ion source are solved, and the equipment is suitable for on-site evidence collection and detection of public safety.

The application discloses a method for determining 14 kinds of mycotoxins in peanuts based on high performance liquid chromatography tandem mass spectrometry, and belongs to the technical field of food detection. The method comprises the steps of solution preparation, test sample solution preparation and high performance liquid chromatography tandem mass spectrometry detection. An acetonitrile-water-acetic acid mixed solution is used as an extraction solution, the peanut sample is ground and crushed, low-temperature ultrasonic extraction, centrifugation, concentration, redissolution and filter membrane filtration are performed, a GL Sciences InertSustain AQ C18 chromatographic column is used for separation, and a triple quadrupole mass spectrometer is used for detection in a multiple reaction monitoring mode. The application realizes synchronous determination of the 14 kinds of mycotoxins, has the advantages of simple pretreatment, high detection efficiency, good sensitivity and high accuracy, and can be used for accurate detection of mycotoxins in peanuts.

The invention belongs to the technical field of drug detection, and relates to a detection method for simultaneously determining two nitrosamine impurities in fluoxetine hydrochloride bulk drugs. Comprising the following steps: dissolving a fluoxetine hydrochloride bulk drug to obtain a test solution, and analyzing and detecting N-nitroso-fluoxetine and N-nitroso-fluoxetine EP impurity A in the test solution by adopting ultra-high performance liquid chromatography-mass spectrometry, in the analysis and detection process, a pentafluorophenyl propyl chromatographic column is adopted, a formic acid aqueous solution is used as a mobile phase A, acetonitrile is used as a mobile phase B, and gradient elution is performed; triple quadrupole mass spectrometry is used as a detector, electrospray ionization source detection is adopted, and multi-reaction detection of MRM is carried out through positive ion mode scanning. The method is stronger in specificity and higher in sensitivity, detection can be completed only in 12 minutes, and the content of two nitrosamine impurities in a sample can be accurately and rapidly detected. The method is of great significance to quality control of fluoxetine hydrochloride bulk drugs and guarantee of medication safety.

The present application relates to a collision cell and a triple quadrupole mass spectrometer having the collision cell. The collision cell comprises a gas supply unit, a straight section having straight electrodes and a curved section having curved electrodes. The gas supply unit is designed for providing an inert gas for the straight section. The straight section is connected to the gas supply unit by a gas channel for inputting the inert gas provided by the gas supply unit into the straight section. The straight electrodes are independent from the curved electrodes and the curved electrodes are located downstream of the straight electrodes. The straight section is designed such that precursor ions input into the straight section collide with the inert gas under the influence of a first transmission electric field and are thereby fragmented to product ions, which are transmitted to the curved section under the influence of the first transmission electric field. The curved section is designed such that precursor ions and product ions input into the curved section are deflected under the influence of a second transmission electric field and are transmitted through the curved section. The straight section and the curved section are arranged in vacuum chambers separated by a partition wall with an aperture.