Method for rapid determination of blood concentration of methotrexate

A technology for methotrexate and blood drug concentration, which is applied to measurement devices, instruments, scientific instruments, etc., can solve the problems of high cost of fluorescence polarization immunoassay, time-consuming microbiological method, and cumbersome sample processing, and achieves low cost and high efficiency. Accuracy, simple preprocessing effect

Inactive Publication Date: 2019-10-11
北京陆道培生物技术有限公司
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Problems solved by technology

Microbial methods are time-consuming and cumbersome; fluorescence polarization immunoassays are expensive, have poor specificity, and are difficult to popularize, and due to the presence of metabolites, antibodies may cross-react, resulting in high measurement results; high-performance liquid chromatography sample processing is cumbersome and the sensitivity is not enough

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  • Method for rapid determination of blood concentration of methotrexate
  • Method for rapid determination of blood concentration of methotrexate
  • Method for rapid determination of blood concentration of methotrexate

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Embodiment Construction

[0017] Embodiments of the present invention are described in detail below, examples of which are shown in the drawings, wherein the same or similar reference numerals designate the same or similar elements or elements having the same or similar functions throughout. The embodiments described below by referring to the figures are exemplary and are intended to explain the present invention and should not be construed as limiting the present invention.

[0018] like Figure 1-4 As shown, the present invention proposes a kind of method for rapidly measuring methotrexate blood drug concentration, comprising the steps:

[0019] (1) First, take the plasma sample to be tested, add 90 μL to 110 μL of ammonium acetate-formic acid aqueous solution, then add 490 μL to 510 μL of methanol solution containing MTX-d3 and vortex for 25 s to 35 s, and then add 9800 r min -1 ~11000r·min -1 Centrifuge at a high speed for 8-12 minutes, and then collect the supernatant.

[0020] (2) Inject the s...

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Abstract

The invention relates to a method for rapid determination of blood concentration of methotrexate. The method comprises the following steps: firstly, adding a to-be-determined plasma sample into ammonium acetate -formic acid water solution, then, adding methanol solution containing MTX-d3 and eddying for 25s-35s, then, performing centrifugal separation, and then, collecting liquid supernatant, injecting the liquid supernatant into a rapid liquid chromatography system to perform separation analysis, then, injecting into a triple quadrupole mass spectrometer detector for detection, further performing data acquisition and processing through a chromatography software, and obtaining the blood concentration of the methotrexate. The method for rapid determination of blood concentration of methotrexate of the invention is rapid and accurate, highly sensitive, simple to operate and low-cost, and is suitable for clinical routine blood concentration monitoring. Preprocessing of the sample is simple, the amount of needed sample is low, so the method is suitable for clinical routine detection and pharmacokinetic study. An interior label is added in a sample preprocessing process, thus, accuracyof MTX quantification is improved, and specificity is high and sensitivity is high.

Description

technical field [0001] The invention belongs to the technical field of drug analysis and detection, and in particular relates to a method for rapidly measuring the blood drug concentration of methotrexate. Background technique [0002] Methotrexate (Methotrexate, MTX) is a folate reductase inhibitor, an anti-folate anti-tumor drug, mainly through the inhibition of dihydrofolate reductase to hinder the synthesis of tumor cell DNA, thereby inhibiting the growth of tumor cells and reproduce. At present, it is widely used in the treatment of malignant tumors such as lymphoid leukemia, lymphoma, chorioepithelial carcinoma and osteosarcoma. There is a close relationship between the plasma concentration of MTX and adverse reactions and curative effects, and there are large individual differences, narrow therapeutic window, and high toxicity. In order to implement MTX administration safely and effectively, it is especially necessary to monitor the blood concentration of MTX after ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/14
CPCG01N30/02G01N30/14
Inventor 王磊刘红星孙文利
Owner 北京陆道培生物技术有限公司
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