The application belongs to the field of biopharmacy and
bioconversion, and particularly relates to a
lipase mutant and application thereof. The
lipase CALB
mutant of the application is obtained by adopting single-
point mutation or combined
mutation on positions 40, 134, 154, 189, 278 and 281 of the
amino acid sequence shown in SEQ ID NO. 1. The
mutant greatly improves the
stereoselectivity in catalyzing the production of (S)-3-
cyclohexene-1-
methyl formate from racemic 3-
cyclohexene-1-
methyl formate. Compared with the traditional chemical method for preparing (S)-3-
cyclohexene-1-
methyl formate, the
lipase CALB mutant provided by the application has high
stereoselectivity, mild
reaction conditions, is friendly to the environment, has reduced requirements on equipment, greatly reduces the production cost, and shows a wide application prospect in industrial application.