11results about How to "Simplified processing steps" patented technology

The application belongs to the field of biopharmacy and bioconversion, and particularly relates to a lipase mutant and application thereof. The lipase CALB mutant of the application is obtained by adopting single-point mutation or combined mutation on positions 40, 134, 154, 189, 278 and 281 of the amino acid sequence shown in SEQ ID NO. 1. The mutant greatly improves the stereoselectivity in catalyzing the production of (S)-3-cyclohexene-1-methyl formate from racemic 3-cyclohexene-1-methyl formate. Compared with the traditional chemical method for preparing (S)-3-cyclohexene-1-methyl formate, the lipase CALB mutant provided by the application has high stereoselectivity, mild reaction conditions, is friendly to the environment, has reduced requirements on equipment, greatly reduces the production cost, and shows a wide application prospect in industrial application.

This invention discloses a ceramic filter media based on Yellow River sediment. By mass percentage, the ceramic filter media comprises the following components: 66-82 wt% Yellow River sediment, 9-15 wt% high-alumina bauxite, 3-7 wt% iron oxide, and 6-12 wt% sodium-based bentonite; the mass ratio of SiO2, Al2O3, and Fe2O3 in the above system is 40-58:8-20:5-12. This application provides a high-value-added utilization method for Yellow River sediment, realizing the resource utilization of Yellow River sediment and solving the problem of Yellow River sediment disposal. The ceramic filter media prepared in this application has wide applications, including in wastewater treatment processes such as aerated biological filters and constructed wetlands. By utilizing solid waste to treat water pollution, it achieves "treating pollution with waste."

The present application belongs to the technical field of pesticide residue detection method, and particularly relates to a method for determining the chlorothalonil residue in fruits and vegetables, comprising the following steps: S1, extracting the fruit and vegetable sample by using an alkaline extraction solution to obtain an extraction solution; S2, adding a garlicin solution to the extraction solution; S3, adding a purification filler to the conversion solution, vortexing, centrifuging, filtering to obtain a to-be-tested solution; S4, using an ultra-high performance liquid chromatography-tandem mass spectrometer to detect the concentrations of mercaptochlorothalonil and 4-hydroxychlorothalonil in the to-be-tested solution, and calculating the total residue of chlorothalonil in the sample. The present application breaks through the traditional idea of inhibiting the conversion of chlorothalonil, actively promotes the complete and quantitative conversion of chlorothalonil into a single derivative through "alkaline extraction + exogenous garlicin catalysis", changes the complex and uncontrollable conversion process into a controllable and quantitative chemical reaction, and avoids the problems of low recovery rate and false negative caused by incomplete or unmeasurable conversion in principle.

The utility model discloses a pathological tissue embedding box, which belongs to the technical field of medical instruments and comprises an embedding box body, an embedding groove is arranged on the top surface of the embedding box body, and a penetrating opening is arranged on the top surface of the embedding box body. The embedding assembly comprises a fixed seat, a convex groove barrier, a movable grid plate and a bottom clamping plate, and the fixed seat is fixedly connected to the top surface of the embedding box body. According to the pathological tissue embedding box, embedding operation is carried out through the embedding box body, mechanical damage caused by multiple times of transfer is reduced, the integrity of tissues is guaranteed, samples are provided for accurate pathological diagnosis, pathological tissue treatment steps are simplified through the integrated operation process, time and manpower input needed by tissue transfer are reduced, and the working efficiency is improved. The number of times of tissue transfer is reduced, the probability of occurrence of errors such as tissue confusion and loss caused by manual operation is effectively reduced, the reliability and stability of a pathological detection result are improved, and the pathological diagnosis work quality is improved.

The invention discloses a blood component monitoring method and device based on a visual identification technology. The device comprises a sensor host and a sensor probe. The sensor host comprises a bottom shell, an image processing mainboard, an elastic buckle, an upper cover, a key, a display unit, a lock ring and a mounting bracket; the image processing mainboard and the elastic buckle are fixed on the bottom shell, the key and the display unit are fixed on the upper cover, and the upper cover and the bottom shell are fixed through the locking ring and are integrally installed on the installation support. The sensor probe comprises a probe shell, a probe fixing plate and a camera. The image of the centrifugal cup in the blood centrifugal separation process is collected through the camera, the blood components are analyzed through the image recognition technology, and accurate recognition of the blood components is achieved; and meanwhile, the mounting position of the centrifugal cup can be automatically monitored. The device is simple in structure, reliable in performance and low in cost, and has higher recognition accuracy and efficiency compared with a traditional photoelectric sensor recognition method.

The invention relates to a preparation method of a bio-based 1, 5-pentamethylene diisocyanate product with yellowing resistance and high stability. A synergistic composite stable system composed of an efficient main antioxidant, an auxiliary antioxidant and a plurality of ultraviolet absorbents / light stabilizers in a scientific proportion is constructed. Wherein the main antioxidant is mainly used for capturing free radicals and blocking an oxidation chain reaction; the auxiliary antioxidant decomposes hydroperoxide to inhibit the hydroperoxide from further initiating the degradation of the material; the ultraviolet stabilizer effectively delays the color change process caused by illumination by absorbing or quenching excited state energy. The preparation method disclosed by the invention has the advantages of high process compatibility, easiness in acquisition of auxiliaries, simple compounding and post-treatment steps and convenience in large-scale popularization; the environmental protection property is outstanding, the yellowing resistance and the storage stability of the product are greatly improved, the normal-temperature closed storage period is prolonged to 6 months or above, the NCO content retention rate is greater than or equal to 96%, and the storage and transportation cost is reduced; the influence of thermal oxidation and photodegradation is resisted, and a complex application environment is adapted.

The utility model discloses a quick dismounting and connecting mechanism and modularization curtain wall belongs to building curtain wall technical field. The curtain wall includes the cross -shaped frame of main installation frame and the side installation frame of both sides and a plurality of curtain wall main parts and connecting assembly. The preliminary positioning is realized to curtain wall main part through the sliding fit of sliding slot and support rod, and the transverse and longitudinal spacing is realized through the bumping of first abutting convex strip, second abutting convex strip and the protruding block on connecting support. The locking assembly adopts the locking wheel with eccentric hole, and the fixing of curtain wall main part is realized after rotating and clamping into locking groove, and the perpendicular direction spacing is enhanced through the clamping of semicircle ring and limiting groove. U-shaped elastic plate and second end cover are used for shielding the gap between curtain wall board, and can adapt to the deformation caused by temperature change. The structure realizes the quick positioning, double locking and gap shielding of curtain wall, improves the installation precision, efficiency and long -term stability, and is convenient to dismount and maintain.

The invention relates to the field of compound preparation, in particular to a method for preparing thiamine hydrochloride. The method comprises the following steps: carrying out contact reaction on thiamine nitrate, ammonium chloride and hydrogen chloride under the condition of an organic solvent, and separating reaction liquid to obtain thiamine hydrochloride solid. Compared with the prior art, ammonium chloride is used for replacing part of hydrochloric acid to participate in the reaction in the thiamine hydrochloride production process, so that the dosage of hydrochloric acid is reduced, equipment corrosion can be reduced, subsequent wastewater treatment steps are simplified, the product yield is not influenced, the content of other impurities is not increased, new production equipment is not added, and the production cost is effectively saved; furthermore, the materials are recycled, so that a more environment-friendly and cost-saving process route can be realized.

This invention discloses an application for mercury ion detection in water environments based on a non-precious metal-doped Raman scattering substrate, belonging to the field of environmental monitoring technology. The invention involves preparing a non-precious metal-doped surface-enhanced Raman scattering substrate; preparing aminated closed-ring Rhodamine 6G; preparing mercury ion standard solutions of different concentrations; and using the prepared surface-enhanced Raman scattering substrate for detection. A standard curve is plotted based on the peak values ​​at characteristic Raman shifts of the obtained standard solutions at different concentrations and the concentrations of the mercury ion standard solutions. The obtained surface-enhanced Raman scattering substrate is used to detect pretreated water samples, and the peak values ​​at characteristic Raman shifts are compared with the standard curve to obtain the concentration of mercury ions in the water sample. This method exhibits high repeatability and stability under different pH values, different salt ion concentrations, different storage temperatures, and different solvents. It also shows specific selectivity for mercury ions, enabling rapid, accurate, and trace detection of mercury ions in water samples.

The application belongs to the technical field of medicine synthesis, and particularly relates to a preparation method of Mupiravir. The selected synthesis route is simple in operation and simplifies the post-reaction treatment steps. After the reaction is completed, the organic phase is separated from the aqueous solution of hydroxylamine sulfate and sodium acetate, the recovery rate of the solvent can be maximized due to the absence of dangerous hydroxylamine sulfate, more importantly, the decomposition of hydroxylamine sulfate is avoided, the production safety is higher, the production is green and environmentally friendly, and the method is more suitable for the popularization of industrialized safe mass production.

The invention discloses a Jingyang Fuzhuan tea year distinguishing method, and belongs to the technical field of tea year distinguishing. Fuzhuan tea samples in different storage years are measured and analyzed by adopting a headspace solid-phase micro-extraction gas chromatograph-mass spectrometer, a Fuzhuan tea storage year predicting model is constructed according to the characteristics of volatile organic compounds and by integrating a machine learning method, and the Jingyang Fuzhuan tea year distinguishing method is used for distinguishing the Jingyang Fuzhuan tea years. And judging the storage year of the Fuzhuan tea through the prediction model. The Jingyang Fuzhuan tea year distinguishing method is simple in treatment step, convenient to operate and suitable for large-scale sample treatment and screening, and the headspace solid-phase microextraction gas chromatograph-mass spectrometer technology is stable and mature; a linear regression model and a support vector machine classification model are established by using the content values of the 10 compounds, and the specific storage year of the Fuzhuan tea can be predicted by combining an error reference value, so that the operation is simple, convenient and objective, the interference of human factors is eliminated, and the accuracy of identifying the storage year of the Fuzhuan tea can be remarkably improved.