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Method for measuring contents of multiple mycotoxins in fresh and raw milk samples

A technology for mycotoxins and fresh milk, applied in measuring devices, instruments, scientific instruments, etc., can solve problems such as inaccurate quantification, false positives, and inability to meet the needs of mycotoxin monitoring and scientific research

Active Publication Date: 2017-09-12
四川省农业科学院分析测试中心
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Problems solved by technology

[0003] At present, the main method for monitoring mycotoxin contamination in milk is enzyme-linked immunosorbent assay, which is widely used in the analysis and detection of mycotoxins, but its further application is limited due to the shortcomings of false positives and inaccurate quantification.
Therefore, quantitative detection methods based on techniques such as thin-layer chromatography, gas chromatography, and high-pressure liquid chromatography have been established, but these methods can only detect one or one type of mycotoxins, which cannot meet the monitoring and monitoring requirements of mycotoxins in the modern dairy industry. Research needs

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  • Method for measuring contents of multiple mycotoxins in fresh and raw milk samples
  • Method for measuring contents of multiple mycotoxins in fresh and raw milk samples
  • Method for measuring contents of multiple mycotoxins in fresh and raw milk samples

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Embodiment Construction

[0022] The principles and features of the present invention are described below in conjunction with examples, which are only used to explain the present invention and are not intended to limit the scope of the present invention.

[0023] 1. Extraction of mycotoxins in fresh milk

[0024] Take 100g of fresh milk added with 14 kinds of mycotoxins, centrifuge at 4500rpm for 10min, and filter with medium-speed filter paper. Weigh 20.0 g of the filtrate, add 20 mL of 10% acetonitrile aqueous solution (volume ratio), shake well and pass through the 3-in-1 polytoxin immunoaffinity column. When finished, wash the affinity column with 20 mL of ultrapure water. After squeezing out the moisture in the affinity column, use 3 mL of methanol to elute, and dry the eluent with nitrogen at 40°C. Use 1.0 mL of 10% methanol aqueous solution (volume ratio) to dissolve the residue, pass through a 0.22 μm PTFE filter membrane, and wait for liquid chromatography test. The 14 kinds of mycotoxins a...

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Abstract

The invention relates to a method for measuring contents of multiple mycotoxins in fresh and raw milk samples. The method comprises the following steps: S1, extracting extracts of the mycotoxins from the fresh and raw milk samples; S2, carrying out liquid chromatographic separation on the extracts of the mycotoxins, so as to obtain elution fractions; S3, carrying out mass spectrum identification on the elution fractions by using a triple-quadrupole mass spectrometer, so as to determine the types and contents of the mycotoxins. After the method is adopted, the fourteen mycotoxins are fully extracted; compared with a single-quadrupole mass spectrometer, the triple-quadrupole mass spectrometer adopted by the method is higher in accuracy, so that a target compound is better separated from interference impurities; therefore, reliable and accurate data can be provided.

Description

technical field [0001] The invention relates to the field of food inspection and quarantine, and more particularly relates to a method for determining the content of various mycotoxins in fresh milk samples. Background technique [0002] Milk has become a necessity in people's lives, especially infant milk is the best substitute for breast milk, but mycotoxins have become one of the main quality and safety risk factors of milk, seriously threatening human health. During milk processing, mycotoxins cannot be degraded by pasteurization or high-temperature sterilization, and mycotoxins can remain intact in milk products. Therefore, mycotoxins in milk have attracted more and more attention. [0003] At present, the main method for monitoring mycotoxin contamination in milk is enzyme-linked immunosorbent assay, which is widely used in the analysis and detection of mycotoxins, but its further application is limited due to the shortcomings of false positives and inaccurate quantifi...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 仲伶俐毛建霏付成平雷绍荣郭灵安欧阳华学雷欣宇李曦罗玲黄世群周虹赵珊
Owner 四川省农业科学院分析测试中心
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