74results about How to "Highly reproducible" patented technology

The present invention relates to a method for preparing a Cannabis plant Δ9-tetrahydrocannabinol isolate from a crude solvent extract of Cannabis plant material. The invention relates further to a Cannabis plant THC isolate comprising Δ9-tetrahydrocannabinol, Cannabinol (CBN) and/or Cannabidiol (CBD) and to a pharmaceutical composition comprising the Cannabis plant THC isolate.

The invention relates to a novel organosilicon carrier system for biological agents that is produced via a simple, stable and reproducible preparation process that is capable of maintaining tertiary protein structure and biological activity of the proteins and/or other biological agents in the mixture, whereby the carrier system is obtainable by mixing one or more organosilicon compounds, selected from the group comprising organosilicon, sugar organosilicon, amino sugar organosilicon compounds, their derivatives, salts and/or the vesicles formed from them, with one or more biological agents, selected from the group comprising antigens, preantigens, antigen conjugates, antibodies, pre-antibodies, antibody conjugates, allergens, allergen extracts, nucleic acids, plasmids, proteins, peptides, pharmaceutical agents, immunologically active substances and/or cosmetics, in solution at a pH value between 7 and 8, preferably 7.4, followed by homogenisation or sonication of the mixture, followed by sterile filtration of the mixture, followed by lyophilisation.

The invention relates to transformed, embryogenic microspores and progeny thereof characterized by being transformed by Agrobacterium tumefaciens, capable of leading to non-chimeric transformed haploid or doubled haploid embryos that develop into fertile homozygous plants within one generation and containing stably integrated into their genome a foreign DNA, said DNA being characterized in that it comprises at least one gene of interest and at least base pairs within the right border sequence of Agrobacterium T-DNA. The invention furthermore relates to a method for the incorporation of foreign DNA into chromosomes of microspores comprising the following steps: a) infecting of embryogenic microspores with Agrobacteria, which contain plasmid carrying a gene of interest under regulatory control of initiation and termination regions bordered by at least one T-DNA border, b) Washing out and killing the Agrobacteria after co-cultivation.

A method and apparatus for nanoscopy comprising a salt microlens. The microlens-based nanoscope comprises a conventional microscope, a microlens, and a XYZ piezoelectric stage is shown (SEE FIG. 1A). The microlens is mounted on a Z-stage and can be driven to accomplish the scanning. The specimen is placed above the microlens which is a plano-convex lens. The set up employed for the Salt Microlens for Ultra-high Resolution Imaging (SAMURI) can use a halogen-tungsten lamp with a dominant wavelength at 600 nm. A magnified virtual image of the specimen is obtained when the distance between microlens and specimen is less than the focal length of the microlens. The virtual image can then be magnified by the microscope and captured by eyes or a CCD camera.

The present invention concerns a new process for depositing a thick compact layer of biomolecules for instance such a layer with thickness in the μm scale and, for depositing a thick compact layer of cells in the μm scale. The deposited layer is made by application of an unbalanced (asymmetrical) alternating voltage polarization between two electrodes to a dissolved biomolecule or cell from low conductivity solutions. The process allows the rapid manufacturing of sensors and the coating of devices with functional cells and biomolecules. Examples are provided on the preparation of functional sensors such as a glucose, a lactose sensor, a hydrogen peroxide sensor and a glutamate sensor. Examples are also provided on the deposition of eukaryoric cells such as saccharomyces cerevisiae. The examples demonstrate a process that can be applied to coat devices with biomolecules and biological cells.

An improved hybrid connector for rapidly, reliably, and reversibly making mixed optical and electrical connections has a plug (103) with one or more centrally located fiber-containing optical ferrules (108) and one or more peripheral electrical contacts (109), and second, a socket (105) with interior electrical contacts (115) and a fiber-bearing central floating optical connector (225). Insertion and rotation of a shaft (107) of the plug into a bore (201) of the socket connects the respective electrical contacts of the shaft and socket, and nearly-simultaneously couples the respective optical fibers of the plug ferrule and socket connector, thus creating a rapid, reversible, and low-cost hybrid connection optimized for both the electrical and optical requirements of a mixed optical and electrical connection.

The present invention provides methods and compositions for the generation of microglial progenitor cells and microglial cells from pluripotent stem cells, such as embryonic stem cells and induced pluripotent stem cells. The present invention also provides cells produced using such methods, and both methods of treatment and methods of drug screening that use such cells. Also provided are various tissue culture media, tissue culture media supplements, and kits useful for the generation of human microglial progenitor cells and human microglial cells.

Devices and methods for measuring body fluid-related metric using spectrophotometry that may be used to facilitate diagnosis and therapeutic interventions aimed at restoring body fluid balance. In one embodiment, the present invention provides a device for measuring a body-tissue water content metric as a fraction of the fat-free tissue content of a patient using optical spectrophotometry. The device includes a probe housing configured to be placed near a tissue location which is being monitored; light emission optics connected to the housing and configured to direct radiation at the tissue location; light detection optics connected to the housing and configured to receive radiation from the tissue location; and a processing device configured to process radiation from the light emission optics and the light detection optics to compute the metric where the metric includes a ratio of the water content of a portion of patient's tissue in relation to the lean or fat-free content of a portion of patient's tissue.

An emergency pulmonary resuscitation device is described. The device of the present invention provides emergency breathing for use in techniques such as CPR, and is preferably capable of interaction with an automatic external defibrillator. The device is easy to operate and provides feedback so that it may be used by persons without medical training. The device also works through a simple action, making it possible for it to be inexpensively manufactured and widely disseminated.

A process for continuous production of profile components of fiber composite material by a forming chain system, an associated profile component and the chain system for its continuous production. The method draws on the forming chain system having at least one forming chain. Here, preform batch of fiber strands, impregnated with matrix material and/or woven fiber webs, knit webs or nonwoven webs, are brought to the system at the working run of the at least one forming chain and are guided through the forming chain system, forming a continuous profile containing the profile component with a variable cross section over the profile length and/or with a profile longitudinal axis with virtually any desired extent. The profile components can be provided with local reinforcements, add-on parts of inserts, for example, for introducing force, as early as during primary forming.

A method and arrangement for creating an impedance controlled printing wiring board, particularly the formation of a structure for high speed printed wiring boards incorporating multiple differential impedance controlled layers. Furthermore, there are provided vias of either through-holes, blind holes and buried holes filled with a conductive paste material to form electrical interconnections with conductive layers of the printed wiring board.

The invention relates generally to novel and substantially improved methods for detecting CpG positions. Particular aspects relate to a method for detection of one or more CpG positions. Said method comprises: providing a sample, binding a protein or peptide onto the DNA of said sample, hybridizing a probe onto the DNA of said sample, detecting a signal determined by said bound proteins or peptides and by said hybridized probes. Thereby one or more CpG positions are detected in a methylation specific and sequence specific manner.

The invention discloses a device for achieving constant-volume proportional dilution. The device comprises a constant-volume tube, a quantifying loop, a mixing bottle, an air pump and a solenoid valve assembly; liquid to be diluted and diluting liquid are pumped to the constant-volume tube and the quantifying loop separately through a pressure difference generated by switching on or switching off the air pump and the solenoid valve assembly, and the mixing bottle is used for mixing the liquid to be diluted and the diluting liquid. The invention discloses a method for achieving constant-volume proportional dilution. A novel constant-volume tube and quantifying loop combining technology and an air pump and solenoid valve combining technology are introduced, and online rapid and precise dilution of various water sources such as ground surface water, sewage and industrial waste water is achieved.

The present invention relates to a high-speed screening apparatus for a Raman analysis-based high-speed multiple drug. The screening apparatus according to the present invention may easily detect a Raman signal using a core-cap-shell nanoparticle which amplifies the Raman signal by 10¹² times and has high reproducibility through Raman spectroscopy in which materials do not interfere with each other and a spectrum has a sharp peak to detect the Raman signal multiple times. Also, since a CCD camera, not a scanner, may be used as the detector, the screening apparatus may multiply screen the drug at a high speed without movement between molecules within a sample. In addition, since multicolors of 5 colors or more may be coated, the screening apparatus may be usefully used for screening various drugs.

Methods for the simultaneous or sequential analysis and quantification of a plurality of antiretroviral analytes in a complex biological matrix by mass spectrometry are disclosed. The methods require minimal sample size, minimal preparation time and allow for rapid through-put. The system is particularly useful in therapeutic drug monitoring.