42 results about "Regulatory control" patented technology

Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating abiotic stress tolerant plants.

The invention relates to transformed, embryogenic microspores and progeny thereof characterized by being transformed by Agrobacterium tumefaciens, capable of leading to non-chimeric transformed haploid or doubled haploid embryos that develop into fertile homozygous plants within one generation and containing stably integrated into their genome a foreign DNA, said DNA being characterized in that it comprises at least one gene of interest and at least base pairs within the right border sequence of Agrobacterium T-DNA. The invention furthermore relates to a method for the incorporation of foreign DNA into chromosomes of microspores comprising the following steps: a) infecting of embryogenic microspores with Agrobacteria, which contain plasmid carrying a gene of interest under regulatory control of initiation and termination regions bordered by at least one T-DNA border, b) Washing out and killing the Agrobacteria after co-cultivation.

A method for building robust model predictive controller universally applicable is presented based on the innate process characteristics independent of the method of control actuation. The method of universal MPC design permits proper configuration of requisite regulatory control loops for measured and unmeasured disturbance rejections consistent with the underlying innate process characteristics and their embedding within the overall process unit model predictive controller. The method of universal MPC design requires that manipulated variables process value based model (PV-based models) be used in control and optimization in place of the customary set point based models (SP-based models) or control output based models (OP-based models). The PV-based models are devoid of the manipulated variables regulatory controllers response and tuning. Based on the PV-based models, an alternate method of MPC called PV-based MPC is presented that is most robust and adaptable of possible three types of MPC. Based on the universal MPC design, the prior art MPC can be adapted to improve its robustness at or near control valve saturation.

The present invention provides methods and DNA constructs for the genetic engineering of plant cells to produce plants which produce substantially seedless fruit in the absence of exogenous growth factors (auxins or cytokinins) and in the absence of pollination. The substantially seedless fruits produced by the methods described herein are about the size of wildtype seeded fruit (or somewhat larger) and these fruits are equal to or superior to the wildtype seeded fruit with respect to solid content and flavor. The seedless fruits of the present invention are produced in transgenic plants which contain and express auxin or cytokinin biosynthetic genes, e.g., tryptophan oxygenase or isopentenyl transferase coding sequences expressed under the regulatory control of GH3 or AGL promoter sequences directing preferential or tissue specific expression of a downstream gene in the ovaries or developing fruit.

This invention provides libraries of expression constructs having different untranslated regions (UTR) from a genome. The expression constructs include transcription regulatory sequences operably linked with a reporter gene and a 5′ UTR, a 3′ UTR or both, wherein the translation of the reporter gene is under the regulatory control of control regions in the UTR sequence. The libraries of this invention are useful for determining the impact of regulatory sequences in the UTRs on translation of open reading frames under a variety of conditions, such as different cellular environments.

A system facilitates detecting an abnormal operation associated with a level regulatory control loop in a process plant. A model for modeling at least a portion of the level regulatory control loop may be utilized with respect to first and second signals associated with regulatory control of a level of material in a tank. The model may include a first regression model in a first range corresponding to a first operating region of the level regulatory control loop. The model may be capable of being subsequently configured to include at least a second regression model in at least a second respective range corresponding to at least a second respective operating region different than the first operating region. The model may generate a prediction of the second signal as a function of first signal. It may be determined whether the second signal significantly deviates from the prediction of the second signal generated by the model. If there is a significant deviation, this may indicate an abnormal operation associated with the level regulatory control loop.

The invention discloses a method and a system for monitoring of certificate information security. The method comprises the steps of acquiring a first label on a certificate, and acquiring a first information code; processing the first information code for reduction, and acquiring a location parameter, an accreditation verification parameter and a third digital signature value; verifying the legitimacy of the location parameter, the accreditation verification parameter and the third digital signature value; verifying whether information of the certificate is valid; acquiring biological features of a certificate holder, and matching and verifying the biological features of the certificate holder and biological features of a legal certificate holder stored in a certificate information database. By adopting the method, the authenticity of the certificate and the certificate holder can be verified by acquiring the first label of the certificate and the biological features of the certificate holder or query can be performed by acquiring a second label via an intelligent mobile terminal. Query and verification of certificate information can be performed, unified regulatory control can be realized, the anti-counterfeit effect is good, the security is high, and the method and the system can be widely applied in the field of certificate regulation.

Proviral plasmids contain a modular gene expression cassette with one or a combination of (i) a wildtype 5′ AAV2 ITR sequence flanked by unique restriction sites that permit ready removal or replacement of said ITR; (ii) a promoter flanked by unique restriction sites that permit ready removal or replacement of the entire promoter sequence; (iii) a polylinker sequence that permits insertion of a gene coding sequence without modification thereof, wherein the gene is operatively linked to, and under the regulatory control of, the aforementioned promoter; (iv) a bovine growth hormone polyadenylation sequence flanked by unique restriction sites that permit ready removal or replacement of said polyA sequence; and (v) a wildtype 3′ AAV2 ITR sequence flanked by unique restriction sites that permit ready removal or replacement of the 3′ ITR. These plasmids enable rapid manipulation of the components of the cassette, e.g., rapid mutation and/or replacement of any component, and thereby increase the efficiency of recombinant viral vector, e.g., rAAV, production.

The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one phytochelatin biosynthetic coding sequence under the regulatory control of the strong ACT2 constitutive promoter or the light-inducible SRS1 promoter and/or a plant-expressible arsenate reductase coding sequence. Optionally the plant expressing the at least one phytochelatin biosynthetic enzyme coding sequence can also express a mercuric ion reductase coding sequence. The transgenic plants are tolerant of heavy metal ions, e.g., cadmium, arsenate and/or mercury, and can accumulate those ions from a contaminated environment, to thus, effect phytoremediation of a contaminated soil or water environment which contains mercury, cadmium and/or arsenate ions.

Expression cassettes causing specific regulatory control of transgene expression in plants, wherein the expression cassettes include regulatory sequences from the MADS gene family for expression of recombinant gene products in the reproductive tissue of plants for the purpose of generating maize plants with altered lignin content of the cobs.

The present invention belongs to the field of life science. Said invention provides a regulatory controlling sequence expressed by zebra fish vitellogenin 1, (vtg 1) gene of sequence 1. Said invention utilizes the transgenic zebra fish experiment to show that said sequence has promoter activity in the zebra fish body interior, and can make downstream gene implement specific expression in liver under the induction of female sex hormone. By adopting the connection of zfvtg 1-1.7 sequence provided by said invention and reporter gene (fluorescin) the fluorescence can be observed in the living body interior of transgenic zebra fish so as to quickly and visually reflect expression of vtg 1.

A recombinant adeno-associated virus (AAV) having an AAV capsid and packaged therein a heterologous nucleic acid which expresses two functional antibody constructs in a cell is described. Also described are antibodies comprising a heavy chain and alight chain from a heterologous antibody. In one embodiment, the antibodies are co-expressed from a vector containing: a first expression cassette which encodes at least a first open reading frame (ORF) for a first immunoglobulin under the control of regulatory control sequences which direct expression thereof; and a second expression cassette which comprises a second ORF, a linker, and a third ORF under the control of regulatory control sequences which direct expression thereof, wherein the second and third ORF encode a second and third immunoglobulin construct. The vector co-expressing these two antibody constructs is in one embodiment an AAV, in which the 5′ and 3′ ITRs flank the expression cassettes and regulatory sequences.

Systems, devices, methods, and program products are provided for operating a medical system that is operable within at least two different medical device regulatory classes. A device control module receives an indicator for performing at least one procedure with a medical device. Based on the indicator, it selects a binary image for booting the control module from among multiple images including a controlled-type and a non-controlled type, which operate in different regulatory classes. A controlled-type image, which may be FDA PMA or other regulatory classes, is verified to be unaltered, and is then used to operate as a regulated medical device. The indicator can be automatic or manual. The indicator may result from connection of a specific medical device or peripheral device, or may be user input.

Human rabies monoclonal antibodies (rabies MAb^P) comprising rabies human MAb S057 heavy chain and light chain subunits are expressed and assembled in plants under the control of two strong constitutive promoters. Additionally, regulatory control elements such as alfalfa mosaic virus untranslated leader sequence and Lys-Asp-Glu-Leu (KDEL) endoplasmic reticulum retention signal were linked at the N- and C-terminus of the heavy chain of human rabies MAb^P, respectively to regulate expression of the rabies MAb^P. Rabies MAb^P was as effective at neutralizing the activity of the rabies virus as the mammalian-derived antibody (MAb^M) or human rabies immunoglobulin (HRIG).

Embodiments of the system, as described herein leverage artificial intelligence, machine-learning, and/or other complex, specific-use computer systems to provide a novel approach for identifying patterns in input data and determine and implement necessary changes to a regulated ML model within the bounds of a regulatory control structure. The system utilizes a collection of machine learning models, either individually or clustered, to process incoming data to determine if specific data should be flagged as irregular or part of the formation of an emerging pattern. The system may intelligently analyze such patterns to determine any regulatory implications that may arise from acting on or adapting to the perceived patterns.

The invention discloses a flight control information monitoring device, comprising a remote controller receiver, a supervisory control platform, a flight control monitor device, a flight controller and a power system, the output end of the remote controller receiver, and the output end of the supervisory control platform respectively connected to the input ends of the flight control monitoring device, the output end of the flight control monitoring device is connected to the input end of the flight controller and the output end of the flight controller is connected to the input end of the flight control monitoring device, the flight control The output end of the monitoring device is connected with the input end of the power system. A flight control information monitoring device provided by the present invention has a simple principle and is convenient to implement. It can not only record the current control information in real time, but also provide a certain basis for the analysis of the cause of the accident of the drone; It provides a third-party control interface and provides a monitoring interface for a unified supervision and control platform.

Although most contemporary and envisioned microfluidic systems are entirely passive or have limited control capabilities, future microfluidic systems require detailed sequenced control of valves and other elements. For this software-control of transport and processes for microfluidic systems under the control of temporal and/or event-driven scripts, script authoring and editing, functional libraries, and other development tools for process and configuration control are proposed with intent towards future standardization. Scripts can respond to external inputs and can comprise conditional-logic, temporal-logic, calculations, hierarchical structure, subroutines, macros, multi-thread and parallel execution operations, procedural calls, interrupts, real-time regulatory control calculations, and element resource allocation.

Scripts can be executed by a script reader, responsive to incoming signals and information, to produce temporal control sequences for operating microfluidic devices, emulation hardware, simulation software, process visualization, etc.

Script authoring/editing tools and readers can record and store information, and can interface to with commercial software, systems, languages, and protocols.

The invention provides a model of a reaction network integrated with regulatory controls related to the reactions. A method is provided for determining a systemic property of a reaction network using a model of the invention. Also provided is a method for modeling changes that occur in a reaction network at various time points due to regulatory events.