32 results about "Phytochelatin" patented technology

The invention provides phytochelatin iron supplement powder which contains iron and has an iron supplementation function and a preparation method of the phytochelatin iron supplementation powder and belongs to the technical field of health-care food. The preparation method includes the steps that fresh pig blood is centrifuged, lower-layer blood cells are obtained, wall breaking is conducted on blood cell homogeneity, hemoglobin is obtained, the hemoglobin is subjected to enzymolysis with alkaline protease, after enzyme deactivation is conducted, enzymatic hydrolysate is subjected to decoloration with a decolorising agent, and liquid supernatant is dried after decoloration, and porcine hemoglobin polypeptide is obtained; chelation with ferrous chloride is conducted, reactant is treated through an ethanol solution, sediment is obtained, suction filtration is conducted repeatedly till filter residues are not separated out any more, freezing and drying are conducted, powder is obtained, and porcine hemoglobin iron phytochelatin is obtained; then 0.2-0.4 part of porcine hemoglobin iron phytochelatin , 30-50 parts of red bean powder, 20-30 parts of jujube powder, 8-10 parts of glucose and 0.3-0.5 part of glucose are fully mixed to be uniform, and the phytochelatin iron supplement powder containing iron and with the iron supplementation function is obtained. Through in-vitro simulation digestion and absorption inspection, the iron absorption rate of a product to which the porcine hemoglobin iron phytochelatin is added is 14-31% higher than that of a product to which ferrous chloride is added, a high iron absorption rate is achieved, and a good iron supplementation function can be achieved.

The present disclosure provides methods, recombinant DNA molecules, recombinant host cells containing the DNA molecules, and transgenic plant cells, plant tissue and plants which contain and express at least one phytochelatin biosynthetic coding sequence under the regulatory control of the strong ACT2 constitutive promoter or the light-inducible SRS1 promoter and / or a plant-expressible arsenate reductase coding sequence. Optionally the plant expressing the at least one phytochelatin biosynthetic enzyme coding sequence can also express a mercuric ion reductase coding sequence. The transgenic plants are tolerant of heavy metal ions, e.g., cadmium, arsenate and / or mercury, and can accumulate those ions from a contaminated environment, to thus, effect phytoremediation of a contaminated soil or water environment which contains mercury, cadmium and / or arsenate ions.

The invention provides a calcium phytochelatin and a preparation method thereof. Schizochytrium limacinum meal protein is used as raw materials; alkaline protease and flavourzyme are used for stepped enzymolysis; then, separation and purification are performed to obtain the purified specific calcium-calcium phytochelatin; the amino acid whole sequence is yl. The prepared phytochelatin can be used for producing a calcium nutrition supplementing agent; the unique chelation mechanism and operation mechanism are realized; the absorption is easy; safety, nontoxicity and no side effect are realized; the price is low; the amino acid and the calcium ions can be supplemented at the same time; the calcium phytochelatin can certainly become the first choice of the calcium supplementing agent. The invention provides a fire-new idea for the application of the schizochytrium limacinum meal protein.

Transgenic plants exhibiting phytochelatin-based heavy metal tolerance and methods of use thereof for bioremediation are disclosed.

The invention belongs to the technical field of biological engineering, relates to a cadmium-enriched genetic engineering strain, and a construction and an application thereof, and in particular relates to the construction of the cadmium-enriched genetic engineering strain and a strain formed by construction of the cadmium-enriched genetic engineering strain, as well as representation of cadmium tolerance, cadmium absorption and other functions of the strain. The genetic engineering strain enriched with heavy cadmium, which is obtained by adopting the construction method disclosed by the invention, is classified and named as Pseudomonasputida KT2440-SpPCS, and the collection number is CCTCCNO: M 2012435. The construction process is mainly as follows: a molecular biology means is used for heterologous expression of Schizosaccharomycespombe phytochelatin in Pseudomonas putida KT2440 for synthesizing an enzyme gene SpPCS, and the genetic engineering strain obtained by the construction method disclosed by the invention can be used as a plant growth promoting bacteria so as to lay a foundation for follow-up research of repair of a heavy metal by coordination and synergy with a plant.

The present invention relates to the discovery of a gene in a bacterium expressing a protein that has an activity similar to plant phytochelatin synthetase. This gene was discovered in the marine γ-proteobacterium Microbulbifer degradans strain 2-40, and is cloned and expressed in the bacterium Escherichia coli. Bacteria expressing this gene are useful in remediation of contaminated solids and liquids and in the extraction of rare and / or valuable metals.

The invention provides a phytochelatin microbial agent and a preparation method thereof. The microbial agent comprises plant active peptides, bacillus natto bacterial liquid, pichia pastoris bacterial liquid, lactobacillus casei bacterial liquid, erycibe obtusifolia benth extract, ulva powder, gallnut powder, ferrous sulfate, potassium fulvic acid and a chelating agent component. In the bacillus natto bacterial liquid, the content of bacillus natto is 40*10<9>CPU / ml; in the pichia pastoris bacterial liquid, the content of pichia pastoris is 16*10<9>CPU / ml; in the lactobacillus casei bacterial liquid, the content of lactobacillus casei is 22*10<9>CPU / ml; in the chelating agent is a mixture consisting of EDTA and calcium superphosphate at a weight ratio of 3.5:1. The content of functional bacterium in the microbial agent is (4.8 to 5.2)*10<9> / g, the content of trace elements is 42 to 45 mg / g, and the infectious microbe rate is 4.5 to 5.2 percent.

The invention relates to a method for preparing an anti-corrosion coating of a fuel gas condenser, and belongs to the technical field of condenser coatings. Aiming at the problems that an existing anti-corrosion organic coating of the fuel condenser is usually poorly combined with a base material, and is high heat resistance and poor in durability, drying and extracting are carried out through aegiceras corniculatum leaves, phytochelatin in aegiceras corniculatum is collected and loaded into a coating, in the coating smearing and curing process, metal on the surface of a pipeline is subjected to complexation through the phytochelatin, the coating is smeared and combined tightly, and meanwhile the heat resistance of the coating is remarkably reduced. The prepared anti-corrosion coating of the fuel gas condenser is highly combined with a base and is high in corrosion resistance, the service life is prolonged by 10% to 25% compared with like products, the preparation steps are simple, and the method is environmentally friendly, safe and free of pollution.

The invention discloses wheat germ protein source zinc phytochelatin and a preparation method thereof. The zinc phytochelatin comprises an amino acid sequence as shown by SEQIDNo.1 and / or SEQIDNo.2. The preparation method comprises the following steps: with degreased wheat germ as a raw material, carrying out protein extraction and enzymatic hydrolysis to obtain zymolyte containing zinc phytochelatin; and then, screening and separating out sequentially to obtain the target product, namely the wheat germ protein source zinc phytochelatin through immobilized zinc ion affinity chromatography, macroporous adsorption resin and reversed-phase high-performance liquid chromatography. The zinc phytochelatin metal has strong chelation, can be used as a carrier for transporting trace elements such as zinc, and improves absorption of the trace elements; particularly, after the phytochelatin and zinc form peptide-zinc chelate, the bioavailability of the peptide-zinc chelate is higher than that of inorganic zinc, the peptide-zinc chelate is easier to digest and absorb, and meets the requirement of an organism on protein. The preparation method is simple and easy to realize large-scale implementation, and the raw materials are low in cost.

The invention discloses a temporary raising method for controlling heavy metal accumulation in mussels. The method comprises the steps of two-time temporary raising, wherein protein ferrous phytochelatin is added in the first temporary raising process, and rhodopseudomonas palustris and kelp are put and cooperate to remove or adsorb heavy metal; immunopotentiator is added in the second temporary raising process, and the immunity of the mussels is improved. According to the temporary raising method for controlling heavy metal accumulation in the mussels, the content of the heavy metal in the mussels is effectively lowered, the survival rate of the mussels is increased, and growth of the mussels is promoted.

The invention discloses a mineral water and GHK phytochelatin-based hair growing agent which comprises 0.00001-1 part of GHK phytochelatin, 0.2 part of preservative and 1-100 parts of mineral water, wherein the mineral water is natural spring water or water prepared from a corresponding ionic compound and water, and water contains active mineral ions; the GHK phytochelatin comprises GHK polypeptide and metal ions. The hair growing agent prepared by mixing the mineral water and the GHK phytochelatin has the advantage that the effect is increased from 50 percent to 100 percent in comparison with that of a hair growing agent singly using the GHK phytochelatin. The active mineral ions in the mineral water have a remarkable synergy to the GHK phytochelatin.

The invention relates to a method for preparing wheat germ polypeptides containing high-affinity calcium through electron beam irradiation in combination with an enzyme method as well as separation and purification. Wheat germ isolate protein is taken as a raw material, a weakly alkaline suspension liquid is prepared from protein and is subjected to enzymolysis with basic protein after being subjected to electron beam irradiation, the wheat germ polypeptides are obtained, a polypeptide mixture is sequentially treated through ultrafiltration, anion-exchange chromatography, gel filtration chromatography, RP-HPLC (reversed-phase high-performance liquid chromatography) and other purification steps, and the polypeptides Phe-Val-Asp-Val-Thr with the highest chelation characteristics are obtained. Obtained calcium phytochelatin is actively absorbed by small intestines in a complex form by the aid of a transportation and absorption mechanism of small peptides, and the calcium utilization rate can be remarkably increased.

The invention provides a preparation method of milk-source calcium phytochelatin. Specifically, cow milk casein is selected as a raw material, trypsin is adopted as a tool enzyme, a calcium-peptide chelate is prepared by adding a calcium salt into zymolyte, then the peptide-calcium chelate is decalcified through EDTA disodium, and thus polypeptide with the calcium chelating activity is obtained. The preparation method is efficient, operation is easy, very high social benefits are achieved, and the prepared milk-source protein calcium phytochelatin has the high calcium chelating activity, is stable in property, is a food-source peptide, is natural and safe and is an ideal functional health-care product additive.

The invention provides a preparation method for micromolecular astragalus phytochelatin. The preparation method comprises the following steps of crushing astragalus and performing decoction with water; then adding pectinase and alpha-L-rhamnosidase, performing stirring for enzymolysis; then adding grosvenor momordica protease, phytase and laccase, and performing continuous enzymolysis; and performing enzyme deactivation on an enzymatic hydrolysate, performing separation and purification, adding soybean peptide, rising the temperature to 45-50 DEG C, performing stirring for 30-45 min to obtainchelating liquid, and concentrating and drying the chelating liquid to obtain the micromolecular astragalus phytochelatin. For the astragalus phytochelatin product prepared with the preparation method, the purity is high, the content of peptide with the peptide molecular weight of 180-500 daltons can reach 99 percent or above; through enzymolysis of compound enzyme, an allergen in astragalus and substances which are difficultly digested and utilized by a human body in the astragalus are degraded, so that the problem that stomach burden is aggravated by taking theastragalus for a long time is effectively avoided, and the micromolecular astragalus phytochelatin can be widely applied to the fields of foods, health care products, drugs and the like.

The invention discloses applications of onion[gamma]-glutamylcysteine ligase AcGCL gene in improving heavy metal tolerance of plants. The sequence of the onion[gamma]-glutamylcysteine ligase AcGCL gene is shown as SEQ ID NO: 1, and the coding amino acid sequence is shown as SEQ ID NO: 2. Studies show that the AcGCL gene can improve the tolerance of Cd by enhancing the synthesis of glutathione (GSH) and phytochelatin (PC) in onions; and the heavy metal tolerance of plants can be improved by enhancing the expression level of the AcGCL gene in the plants, or the AcGCL gene is utilized to breed new heavy metal-tolerant transgenic plant varieties over-expressing the AcGCL gene.

Transgenic plants exhibiting phytochelatin-based heavy metal tolerance and methods of use thereof for bioremediation are disclosed.

The invention relates to the technical field of folium isatidis deep processing, in particular to a preparation method of small molecule folium isatidis phytochelatin. According to the method, foliumisatidis is taken as the primary raw material, and a small molecule phytochelatin product is obtained after steaming processing, neutral protease enzymolysis, solid liquid separation, purification, nanofiltration, polypeptide chelation and drying are conducted. The method has the advantages that the production cycle is short, the cost is low, the method has no pollution on the environment, the safety performance of a product is high, the purity is high, the molecular weight of the product is less, utilization rate of the effective ingredient is high, the effect comes up fast, and the small molecule folium isatidis phytochelatin can be applied to the fields of biological feed additives, drugs and the like.

The invention discloses a reed phytochelatin gene PaPCS, a plant expression vector of the gene PaPCS and application of the gene to the cultivation of heavy metal-resistant plants. A method for improving heavy metal accumulation and heavy metal resistance capacities of lawn grass, namely Festuca arundinacea Tall Fescue by using the trans-PaPCS gene can be widely used for cultivating plant varieties which have large heavy metal-resistant biomass and grow fast. Through the experiment, the capacity of heavy metal enrichment of the transgenic plant is improved compared with that of a non-transgenic plant.

The invention relates to a method for preparing thamnaconus modestus skin zinc phytochelatin. The method specifically comprises the following steps: by taking thamnaconus modestus skin as a raw material, removing non-collagen and minerals in the fish skin by using acid and alkali, extracting fish skin gelatin, performing enzymolysis on the fish skin gelatin by using neutral protease and trypsin, performing ultrafiltration, immobilized zinc ion affinity chromatography and reversed-phase high-performance liquid chromatography separation purification, thereby obtaining zinc phytochelatin Gly-Pro-Tyr-Gly-Pro-Phe-Gly-Pro-Trp-Gly (GPYGPFGPWG). ESI-MS testing shows that the molecular weight of thamnaconus modestus skin zinc phytochelatin is 1034.09Da.

A composition for transportation of phytochelatin including two DNA molecule encoding two members of ABCC (multidrug resistance-associated protein (MRP)) subfamily of ATP-binding cassette (ABC) transporter protein in a plant is provided. The composition for transportation of phytochelatin can be useful in accumulating and sequestering phytochelatin alone or in combination with a harmful substance by transporting the phytochelatin and / or harmful substance into the vacuole of a cell, thereby reducing a content of the toxic substance from cytosol, and also reducing it from translocation from the root to the shoot of a plant. Therefore, the DNA molecule can be useful to develop a crop in which the toxic substance present in edible parts, such as leaves, stems and fruits, of the plant is reduced in content. Furthermore, the DNA molecule can be useful to develop inedible plants for phytoremediation or phytoextraction due to an increase in accumulated amount of and resistance against arsenic, an arsenic compound and cadmium, thereby making it possible to achieve phytoremediation or phytoextraction in an environmentally friendly and economic manner.

The invention provides a method for improving tolerance of soybeans to petroleum and lead contaminated soil by applying photosynthetic bacteria, and relates to a method for improving soil tolerance. The method comprises the following steps of: before soybean seeds are sowed, spraying dilute bacterial agent to soil, diluting bacterial agent, soaking the seeds in the dilute bacterial agent and sowing the seeds; after the seeds are sowed, infiltrating dilute bacteria; and in the soybean growth process, monitoring the content of soybean chlorophyll every week from the seedling stage: selecting typical plants in a population, selecting fully-unfolded leaves at the topmost node positions, determining the content of the chlorophyll, or from the podding starting stage to the seed filling stage, respectively taking and spraying calcium chloride solution on leaf surfaces once, and then taking, diluting and spraying photosynthetic bacteria agent stock solution once. The method for improving the tolerance of the soybeans to the petroleum and lead contaminated soil by applying the photosynthetic bacteria has the advantages that the use cost is low, the process is safe, the environmental friendliness after use is good, the contents of the chlorophyll and carotenoid are increased very obviously, the plants are high, the stalks are thick, the dry weight and the fresh weight are greatly improved, the resistance indexes such as contents of proline, POD (peroxidase) and phytochelatin are improved, the content of malonaldehyde is reduced, the soybeans can tolerate the petroleum and lead contaminated soil, the physical and chemical properties of the soil can be remarkably improved and the soil fertility is improved.

The present disclosure relates to a method and system for the in-situ detection of cadmium chelates in a plant. The method includes: preparing cadmium chloride and phytochelatin (PC) 2 samples with different concentrations; using terahertz time-domain spectroscopy (THz-TDS) to determine a terahertz (THz) reflection image for the cadmium chloride and PC 2 samples; building a cadmium chloride content detection model according to first spectral data; building a PC 2 content detection model according to second spectral data; determining an optimal mixed sample according to the cadmium chloride content detection model and the PC 2 content detection model; building a Cd—PC2 content detection model according to the optimal mixed sample; and using the Cd—PC2 content detection model to determine a cadmium chelate content in a to-be-tested plant. The present disclosure can quickly and in-situ detect the heavy metal accumulation capacity of a plant.

The invention provides a phytochelatin microbial agent and a preparation method thereof. The microbial agent comprises plant active peptides, bacillus natto bacterial liquid, pichia pastoris bacterial liquid, lactobacillus casei bacterial liquid, erycibe obtusifolia benth extract, ulva powder, gallnut powder, ferrous sulfate, potassium fulvic acid and a chelating agent component. In the bacillus natto bacterial liquid, the content of bacillus natto is 40*10<9>CPU / ml; in the pichia pastoris bacterial liquid, the content of pichia pastoris is 16*10<9>CPU / ml; in the lactobacillus casei bacterial liquid, the content of lactobacillus casei is 22*10<9>CPU / ml; in the chelating agent is a mixture consisting of EDTA and calcium superphosphate at a weight ratio of 3.5:1. The content of functional bacterium in the microbial agent is (4.8 to 5.2)*10<9> / g, the content of trace elements is 42 to 45 mg / g, and the infectious microbe rate is 4.5 to 5.2 percent.

The invention relates to the technical field of folium isatidis deep processing, in particular to a preparation method of small molecule folium isatidis phytochelatin. According to the method, foliumisatidis is taken as the primary raw material, and a small molecule phytochelatin product is obtained after steaming processing, neutral protease enzymolysis, solid liquid separation, purification, nanofiltration, polypeptide chelation and drying are conducted. The method has the advantages that the production cycle is short, the cost is low, the method has no pollution on the environment, the safety performance of a product is high, the purity is high, the molecular weight of the product is less, utilization rate of the effective ingredient is high, the effect comes up fast, and the small molecule folium isatidis phytochelatin can be applied to the fields of biological feed additives, drugs and the like.

The present invention relates to a composition for transporting phytochelatins comprising two DNA molecules encoding two members of the ABCC (multidrug resistance-associated protein (MRP)) subfamily of ATP-binding cassette (ABC) transporters in plants. By transporting phytochelatins and / or noxious substances into cell vacuoles, the composition for transporting phytochelatins can be used alone or in combination with noxious substances to accumulate and sequester phytochelates, thereby reducing toxic substances in the cytoplasm. It also reduces the content of toxic substances that migrate from the roots to the stems of plants. Thus, said DNA molecules can be used to develop crops in which the content of toxic substances present in the edible parts of the plant, such as leaves, stems, fruits, is reduced. Furthermore, the DNA molecules can be used to develop inedible plants for phytoremediation or phytoextraction, and to increase the accumulation and resistance of plants to arsenic, arsenic compounds, and cadmium, possibly in an environmentally friendly and economically clean manner Phytoremediation or Phytoextraction.

The present disclosure relates to a method and system for the in-situ detection of cadmium chelates in a plant. The method includes: preparing cadmium chloride and phytochelatin (PC) 2 samples with different concentrations; using terahertz time-domain spectroscopy (THz-TDS) to determine a terahertz (THz) reflection image for the cadmium chloride and PC 2 samples; building a cadmium chloride content detection model according to first spectral data; building a PC 2 content detection model according to second spectral data; determining an optimal mixed sample according to the cadmium chloride content detection model and the PC 2 content detection model; building a Cd—PC2 content detection model according to the optimal mixed sample; and using the Cd—PC2 content detection model to determine a cadmium chelate content in a to-be-tested plant. The present disclosure can quickly and in-situ detect the heavy metal accumulation capacity of a plant.

The invention relates to the technical field of deep processing of garlics, in particular to a preparation method for micromolecule garlic phytochelatin. Garlics are decocted and enzymed by amylase and a saccharifying enzyme, glutathione is added, then aspergillus oryzae neutral protease and kiwifruit protease are added for enzyming, and after enzymatic hydrolysate is inactivated, separation purification and nanofiltration are conducted; finally, soybean oligopeptide is added for chelating, and the micromolecule garlic phytochelatin is obtained. Compared with the prior art, protein in the garlics can be fully degraded, the rate of extracting micromolecule peptide is increased, other active substances and the peptide are subjected to efficient chelating, and the bioavailability of the micromolecule garlic phytochelatin is improved.

The present disclosure provides a method of producing transgenic plants which are resistant to at least one metal ion by transforming the plant with a recombinant DNA comprising a nucleic acid encoding a bacterial arsenic reductase under the control of a plant expressible promoter, and a nucleic acid encoding a nucleotide sequence encoding a phytochelatin biosynthetic enzyme under the control of a plant expressible promoter. The invention also relates a method of phytoremediation of a contaminated site by growing in the site a transgenic plant expressing a nucleic acid encoding a bacterial arsenate reductase and a nucleic acid encoding a phytochelatin biosynthetic enzyme.