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Method for preparing wheat germ polypeptides containing high-affinity calcium through electron beam irradiation in combination with enzyme method as well as separation and purification

A technology of electron beam irradiation and enzymatic separation, which is applied in the direction of peptides and fermentation, can solve the problems of limiting the comprehensive utilization of wheat germ protein, poor solubility, environmental pollution, etc., to shorten the time of enzymatic hydrolysis, improve the degree of hydrolysis, The effect of increasing the chelation rate

Inactive Publication Date: 2017-06-16
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the solubility of globulin, glutenin and gliadin is not good, which limits the comprehensive utilization of wheat germ protein to a certain extent, resulting in waste of resources and environmental pollution.

Method used

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  • Method for preparing wheat germ polypeptides containing high-affinity calcium through electron beam irradiation in combination with enzyme method as well as separation and purification
  • Method for preparing wheat germ polypeptides containing high-affinity calcium through electron beam irradiation in combination with enzyme method as well as separation and purification
  • Method for preparing wheat germ polypeptides containing high-affinity calcium through electron beam irradiation in combination with enzyme method as well as separation and purification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Extraction of wheat germ protein isolate: defatted wheat germ powder (purchased from Anyang Mantianxue Co., Ltd., Henan Province) was dispersed in deionized water at a material-to-liquid ratio of 1:10 (w / v), and the pH of the dispersion was kept constant at pH9. 5. Stir at a constant temperature of 40°C for 2 hours, centrifuge at 5000rpm for 10 minutes to obtain a supernatant, adjust the supernatant to pH 4.0 to precipitate protein, wash the protein twice with water and adjust the pH to neutral, freeze-dry to obtain wheat germ protein isolate.

[0024] (2) Wheat germ protein isolate powder and deionized water are mixed according to the material-to-liquid ratio of 40% (w / v), and lye is added to form a weakly alkaline suspension (pH8.0), which is sealed in a transparent In a sealed bag, the thickness is controlled at 1-2cm.

[0025] (3) The protein suspension is placed on the conveyor belt, and irradiated under the electron accelerator, the irradiation dose is 1kGy, a...

Embodiment 2

[0032] (1) Extraction of wheat germ protein isolate: defatted wheat germ powder (purchased from Anyang Mantianxue Co., Ltd., Henan Province) was dispersed in deionized water at a material-to-liquid ratio of 1:10 (w / v), and the pH of the dispersion was kept constant at pH9. 5. Stir at a constant temperature of 40°C for 2 hours, centrifuge at 5000rpm for 10 minutes to obtain a supernatant, adjust the supernatant to pH 4.0 to precipitate protein, wash the protein twice and adjust the pH to neutral, freeze-dry to obtain wheat germ protein isolate.

[0033] (2) Wheat germ protein isolate powder and deionized water are mixed according to the material-to-liquid ratio of 10% (w / v), and lye is added to form a weakly alkaline suspension (pH8.5), which is sealed in a transparent In a sealed bag, the thickness is controlled at 1-2cm.

[0034] (3) The protein suspension is placed on a conveyor belt and irradiated under an electron accelerator with a dose of 5 kGy and an irradiation conditi...

Embodiment 3

[0041] (1) Extraction of wheat germ protein isolate: defatted wheat germ powder (purchased from Anyang Mantianxue Co., Ltd., Henan Province) was dispersed in deionized water at a material-to-liquid ratio of 1:10 (w / v), and the pH of the dispersion was kept constant at pH9. 5. Stir at a constant temperature of 40°C for 2 hours, centrifuge at 5000rpm for 10 minutes to obtain a supernatant, adjust the supernatant to pH 4.0 to precipitate protein, wash the protein twice with water and adjust the pH to neutral, freeze-dry to obtain wheat germ protein isolate.

[0042] (2) Wheat germ protein isolate powder and deionized water are mixed according to the material-to-liquid ratio of 25% (w / v), and lye is added to form a weakly alkaline suspension (pH7.5), which is sealed in a transparent In a sealed bag, the thickness is controlled at 1-2cm.

[0043] (3) The protein suspension is placed on a conveyor belt and irradiated under an electron accelerator with a dose of 10 kGy and an irradia...

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Abstract

The invention relates to a method for preparing wheat germ polypeptides containing high-affinity calcium through electron beam irradiation in combination with an enzyme method as well as separation and purification. Wheat germ isolate protein is taken as a raw material, a weakly alkaline suspension liquid is prepared from protein and is subjected to enzymolysis with basic protein after being subjected to electron beam irradiation, the wheat germ polypeptides are obtained, a polypeptide mixture is sequentially treated through ultrafiltration, anion-exchange chromatography, gel filtration chromatography, RP-HPLC (reversed-phase high-performance liquid chromatography) and other purification steps, and the polypeptides Phe-Val-Asp-Val-Thr with the highest chelation characteristics are obtained. Obtained calcium phytochelatin is actively absorbed by small intestines in a complex form by the aid of a transportation and absorption mechanism of small peptides, and the calcium utilization rate can be remarkably increased.

Description

technical field [0001] The invention relates to the technical field of preparation of functional peptides and intensive processing of agricultural products, in particular to a method for calcium chelating peptides derived from wheat germ protein. Background technique [0002] Due to the high proportion of plant foods in my country's dietary structure, calcium deficiency often occurs, especially in children, pregnant women and the elderly. Therefore, calcium supplementation has become an important topic in the research of domestic scholars. At present, calcium supplements on the market are mainly inorganic calcium, organic calcium, and amino acid calcium. Inorganic calcium (such as calcium carbonate, calcium phosphate, calcium chloride, etc.), such calcium supplements have poor stability and solubility in the gastrointestinal tract, low absorption and utilization, and stimulate the stomach; organic calcium (such as calcium lactate, glucose calcium citrate, calcium citrate, e...

Claims

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Application Information

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IPC IPC(8): C12P21/06C07K7/06C07K1/36C07K1/34C07K1/18C07K1/16
CPCC07K7/06C12P21/06
Inventor 王莉陈正行丁媛媛
Owner JIANGNAN UNIV
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